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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Rekayasa Proses JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Jurnal Ilmu Lingkungan Journal of Marine Research Microbiology Indonesia OSEATEK DIPOIPTEKS: Jurnal Ilmiah Mahasiswa Undip Jurnal Matematika & Sains Prosiding Indonesian Journal of Pharmaceutical Science and Technology Proceeding Seminar LPPM UMP Tahun 2014 Indonesian Journal of Biotechnology Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Marine Research in Indonesia Biosaintifika: Journal of Biology & Biology Education Jurnal Rekayasa Proses Indonesian Journal of Energy Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding University Research Colloquium Prosiding Konferensi Nasional Penelitian Matematika dan Pembelajarannya

Ocky Karna Radjasa

Badan Riset Dan Inovasi Nasional

Author-ID : 295934

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences Other

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Maggot Meal (Hermetia illucens) Substitution on Fish Meal as Source of Animal Protein to Growth, Feed Utilization Efficiency, and Survival Rate of Milkfish (Chanos chanos) Herawati, Vivi Endar; Pinandoyo, Pinandoyo; Windarto, Seto; Hariyadi, Putut; Hutabarat, Johannes; Darmanto, YS; Rismaningsih, Nurmanita; Prayitno, Slamet Budi; Radjasa, Ocky Karna
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

High protein content in maggot is one of the advantages for increasing fish growth through artificial feed. This study aimed to find the best optimization and feed formulation for fish meal substitution with maggot meal on growth, feed utilization efficiency, and survival rate of milkfish (C. chanos). Milkfish (C. chanos) with an average weight of 0.62Â±0.01 gram/fish was used as test fish. Feeding is carried out at 07.00 a.m., 12.00 p.m., and 17:00 p.m. through fixed feeding rate method. The tested fish was kept for 42 days with a stocking density of 1 fish/l. The experimental design used was a completely randomized design with five treatments and three replications. The treatments which had been done were fish meal substitution with maggot meal as follows: A (0%), B (25%), C (50%), D (75%), and E (100%). The research parameters included total feed consumption (TFC), feed utilization efficiency (FUE), protein efficiency ratio (PER), relative growth rate (RGR), survival rate (SR), and water quality. The results showed that the fish meal substitution with maggot meal had a significant effect (p<0.05) on FUE, PER, RGR and had no significant effect (p>0.05) on TFC and SR. The best treatment of each treatment is in treatment C with a composition of 50% maggot meal substitution on fish meal which resulted in a TFC value of 40.17Â±4.58, FUE of 27.51Â±0.77, PER of 0.83Â±0.03%, and RGR of 2.34Â±0.10.

Antibacterial Activities of the Extracts of Sponge-Associated Fungus Trichoderma longibrachiatum against Pathogenic Bacteria Sedjati, Sri; Ambariyanto, Ambariyanto; Trianto, Agus; Supriyantini, Endang; Ridlo, Ali; Bahry, Muhammad Syaifudien; Wismayanti, Gita; Radjasa, ocky Karna; McCauley, Erin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 15, No 2 (2020): August 2020
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v15i2.438

This study aims to explore the antibacterial potential of a sponge-associated fungus Trichoderma longibrachiatum isolated from Ternate waters, North Maluku, Eastern Indonesia. Various culture media were used to stimulate the production of secondary metabolites in T. longibrachiatum. The isolate was cultured in various media for 6-9 days. Then, the antibacterial activities of the ethyl acetate extracts were assayed against pathogenic bacteria of Multi-Drug Resistant (MDR) strain (Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, and Bacillus cereus). The results showed that all extracts had similar profiles on the thin layer chromatography. However, two of the most potent extracts were produced from the PCA and MEA media for 9 days. These extracts inhibited methicillin-resistant S. aureus (MRSA) (12.48 mm and 12.27 mm); B. cereus (12.11 mm and 12.12 mm); K. pneumoniae (12.40 mm and 10.76 mm); and P. aeruginosa (11.59 mm and 8.69 mm) at concentrations 500 mg/disc. In conclusion, the fungus T. longibrachiatum that was cultured in PCA and MEA media had the potential to produce antibacterial compounds against MDR pathogens and both had similar compounds.Â Meanwhile, theÂ ethyl acetate extracts from fungus cultured in the TPA and TA media were inactive against all tested bacteria

Antibacterial Property of a Coral-Associated Bacterium Pseudoalteromonas luteoviolacea Against Shrimp Pathogenic Vibrio harveyi (In Vitro Study) OCKY KARNA RADJASA; TORBEN MARTENS; HANS- PETER GROSSART; AGUS SABDONO; MEINHARD SIMON; TONNY BACHTIAR
HAYATI Journal of Biosciences Vol. 12 No. 2 (2005): June 2005
Publisher : Bogor Agricultural University, Indonesia

A coral-associated bacterium was successfully screened for secondary metabolites production based on PCR amplification of the nonribosomal peptide synthetase gene and was identified as closely related to Pseudoalteromonas luteoviolacea based on its 16S rDNA.The bacterium was found to inhibit the growth of shrimp pathogenic bacterium tested, Vibrio harveyi. To characterize the inhibiting metabolite, a 279 bp long DNA fragment was obtained and the deduced amino acid sequence showed conserved signature regions for peptide synthetases and revealed a high similarity to NosD (40% identity), a multifunctional peptide synthetase from Nostoc sp. GSV224, and NdaB (44% identity), a peptide synthetase module of Nodularia spumigena.

Nutritional Content of Artemia sp. Fed with Chaetoceros calcitrans and Skeletonema costatum VIVI ENDAR HERAWATI; JOHANNES HUTABARAT; OCKY KARNA RADJASA
HAYATI Journal of Biosciences Vol. 21 No. 4 (2014): December 2014
Publisher : Bogor Agricultural University, Indonesia

Artemia sp. is a natural food with high protein content, especially amino acid. Nowadays, Indonesia still relies on import for its supply. Hence, the utilization of local Artemia sp. as an alternative to the imported product is recommended as it contains more protein and less expensive. The advantages of local Artemia sp. is its better crystal quality as it is still fresh. It also provides better income for salt farmers because waste water from salt farm can be used to culture Artemia sp.. This research is aimed at determining the quality of locally-produced Artemia sp., by evaluation of its essential amino acid and fatty acid profiles after treatments. Our results indicated that Artemia sp. cysts with good quality were produced after 8 hours and Artemia sp. reaches a hatching rate of 1,320,000 cysts (95%) after 27 hours. We also found an indication that the best feed concentration was a mix of 60% Chaetoceros calcitrans with 40% Skeletonema costatum. Fatty acid profile analyses showed that the highest SAFA (12.86%) and PUFA (29.91%) were gained after feeding with Chaetoceros calcitrans, whereas the highest HUFA (4.93%) was gained after feeding with Skeletonemacostatum. Essential amino acid profile analyses revealed the highest content of amino acid (18912.62 ppm) was after feeding with a combination of Chaetoceros calcitrans and Skeletonema costatum. Finally, the proper water quality during research was at 25-30 oC of temperature, 30-31 ppt of salinity, pH 7.8-8.9, and DO was at 3.0-4.4 mg/L.

Growth and Survival Rate of Tilapia (Oreochromis niloticus) Larvae Fed by Daphnia magna Cultured With Organic Fertilizer Resulted From Probiotic Bacteria Fermentation Vivi Endar Herawati; Johannes Hutabarat; . Pinandoyo; Ocky Karna Radjasa
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

Daphnia magna is a potential feed for fish. The aim of this research was to find the best treatment and effect of D. magna culture addition from fermented organic fertilizer, to growth and survival rate of Oreochromis niloticus larvae. There were five treatments, each with three repetitions used in the study. All treatments used chicken dung, and different combinations of rice bran, coconut oilcake waste and tilapia larvae. Feeding on tilapia was given by ad libitum method for five times a day until 14 days. Water quality during the research was maintained at temperature 28–29°C, DO 0.3 ppm and pH 8.1–8.2. Observed variables include relative growth rate, survival rate, food consumption rate and water quality. Our results showed that D. magna cultured by fermented organic fertilizer for tilapia larvae (O. niloticus) had high significant effect (p < 0.01) on the relative growth rate and survival rate. Treatment of D. magna cultured by 1.2 g/L chicken manure, 0.9 g/L rice bran and 0.3 g/L coconut oilcake showed the highest value on the relative growth rate (10.86%); survival rate (98.46%) and food consumption at first week (106.43%) and second week (152.76%).

Maggot Meal (Hermetia illucens) Substitution on Fish Meal as Source of Animal Protein to Growth, Feed Utilization Efficiency, and Survival Rate of Milkfish (Chanos chanos) Vivi Endar Herawati; Pinandoyo Pinandoyo; Seto Windarto; Putut Hariyadi; Johannes Hutabarat; YS Darmanto; Nurmanita Rismaningsih; Slamet Budi Prayitno; Ocky Karna Radjasa
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

High protein content in maggot is one of the advantages for increasing fish growth through artificial feed. This study aimed to find the best optimization and feed formulation for fish meal substitution with maggot meal on growth, feed utilization efficiency, and survival rate of milkfish (C. chanos). Milkfish (C. chanos) with an average weight of 0.62±0.01 gram/fish was used as test fish. Feeding is carried out at 07.00 a.m., 12.00 p.m., and 17:00 p.m. through fixed feeding rate method. The tested fish was kept for 42 days with a stocking density of 1 fish/l. The experimental design used was a completely randomized design with five treatments and three replications. The treatments which had been done were fish meal substitution with maggot meal as follows: A (0%), B (25%), C (50%), D (75%), and E (100%). The research parameters included total feed consumption (TFC), feed utilization efficiency (FUE), protein efficiency ratio (PER), relative growth rate (RGR), survival rate (SR), and water quality. The results showed that the fish meal substitution with maggot meal had a significant effect (p<0.05) on FUE, PER, RGR and had no significant effect (p>0.05) on TFC and SR. The best treatment of each treatment is in treatment C with a composition of 50% maggot meal substitution on fish meal which resulted in a TFC value of 40.17±4.58, FUE of 27.51±0.77, PER of 0.83±0.03%, and RGR of 2.34±0.10.

Uji Aktivitas Antibakteri Pigmen Karotenoid Dari Bakteri Simbion Karang Lunak Sarcophyton sp. Terhadap Pertumbuhan Bakteri Patogen Staphylococcus aureus ATCC 25923 Awang Surya Wiguna; Lia Kusmita; Ocky Karna Radjasa
Indonesian Journal of Pharmaceutical Science and Technology Vol 3, No 3 (2016)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Mikroba penghasil antibiotik dapat berupa fungi maupun bakteri yang bersimbiosis pada organisme lain. Pigmen karotenoid dari bakteri simbion karang lunak merupakan mikroorganisme yang berpotensi sebagai antibakteri. Tujuan penelitian ini untuk mengetahui aktivitas antibakteri ekstrak pigmen karotenoid dari bakteri simbion karang lunak Sarcophyton sp. terhadap pertumbuhan bakteri patogen Staphylococcus aureus ATCC 25923 serta mengetahui perbedaan aktivitas antibakteri ekstrak pigmen karotenoid pada konsentrasi 0,5 %, 0,75 %, dan 1 % dengan metode sumuran. Bakteri yang menghasilkan pigmen karotenoid dibiakkan dan diidentifikasi dengan mengisolasi DNA untuk dilakukan PCR dan mensequen hasilnya. Hasil penelitian aktivitas antibakteri dengan rata – rata diameter zona hambat pada konsentrasi 0,5 % sebesar 0,678 cm, konsentrasi 0,75 % sebesar 0,978 cm, konsentrasi 1 % sebesar 1,416 cm serta diameter zona hambat kontrol positif amoksisilin trihidrat sebesar 1,875 cm.

Isolation and Screening of Antimicrobial Producing-Actinomycetes Symbionts in Nudibranch R. Riyanti; Jaka Widada; Ocky Karna Rajasa
Indonesian Journal of Biotechnology Vol 14, No 1 (2009)
Publisher : Universitas Gadjah Mada

The aims of this study were to isolate and to screen actinomycetes associated with sea slug which have the ability to produce antimicrobial compound, especially against MDR strains. Actinomycetes were isolated from nudibranchs collected from Bandengan coastal waters and the Panjang island, Jepara, Central Java. Actinomycete isolates were assayed for their antimicrobial activity against MDR strains (MDR 6 E. coli, MDR 7 Enterobacter sp., MDR 13 Proteus sp., MDR 14 Staphylococcus sp.). The genetic diversity of the active isolates was analyzed by using repetitive DNA fingerprinting. Antimicrobial activity was also performed on the ethyl acetate bacterial extract. The amplification of Polyketide Synthase-I (PKS-I) and Non-Ribosomal Peptide Synthetase (NRPS) genes was carried out to estimate the genetic potency of actinomycetes. The most active actinomycete isolate was sequenced based on 16S rDNA approach. General profile of antimicrobial substances was analyzed by using Thin Layer Chromatography (TLC). A total 27 isolates were obtained from nudibranchs Jorunna sp. and 12 isolates from Chromodoris sp. Ten isolates exhibited antimicrobial activity. Five representative isolates were selected based on rep-PCR analysis. Three ethyl acetate extracts exhibited antimicrobial activity against MDR 7, MDR 13, and MDR 14, except MDR 6. NPC 8 isolates significantly inhibited the growth of the tested strain and amplified NRPS gene fragment. Molecular identification revealed that isolate NPC 8 closely related to Streptomyces sp with a high homology of 96%.

Evaluation of antimicrobial activity and identification of yellow pigmented marine sponge-associated fungi from Teluk Awur, Jepara, Central Java Mada Triandala Sibero; Desy Wulan Triningsih; Ocky Karna Radjasa; Agus Sabdono; Agus Trianto
Indonesian Journal of Biotechnology Vol 21, No 1 (2016)
Publisher : Universitas Gadjah Mada

Marine sponge associated fungi are known as potential source of metabolites with various biological activities. Natural pigment is one of metabolite which produced by microorgisms. Several researches reported the antimicrobial activity from natural pigment. Unfortunatelly there are lack of information about marine fungi natural pigment and its producer. The aims of this research were to identify yellow pigmented Indonesian marine sponge-associated fungi, to extract the pigment, and to study the antimicrobial activity of the pigment against clinical MDR bacteria and clinical pathogenic fungi. Sponge associated-fungus isolate MT23 was successfully identified as Trichoderma parareesei. The fungal pigment could be extracted only in methanol with yield 6,22±0,29%. The pigment could inhibitted S. typhi and E. coli MDR strains. The biggest antibacterial activity was shown by concentration 1000µg/mL against S. typhi with inhibition zone was 4.03±0.06 mm.

COMPOSITION AND ANTIBACTERIAL ACTIVITIES OF MARINE SPONGES FROM DIFFERENT SEAGRASS ECOSYSTEMS IN KEPULAUAN SERIBU WATERS, JAKARTA Meutia Samira Ismet; Dietriech G. Bengen; Ocky Karna Radjasa; Mujizat Kawaroe
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 2 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

A seagrass ecosystem has high productivity that supports many species of associated invertebrates, including sponges. However, seagrass beds with different habitat condition, including seagrass species composition and architectural morphotypes could affect the associative sponge in the ecosystem. This study is aimed to find out about sponge species composition in different seagrass beds. The observation was conducted in two seagrass ecosystems at the east (site 1) and at the southeast (site 2) of Pramuka Island, Kepulauan Seribu, near Jakarta Bay, Indonesia. The Belt Transect was used to assess the density of sponges on the seagrass ecosystem, which was placed parallel to the coastline. The sponges species has higher number at the east than the southeast, with both sites was dominated by Spirastrella sp. and Agelas conifera. Correspondence Analysis (CA) results showed that sponges community has close interaction with seagrass abundance especially Cymodocea rotundata and Enhalus acoroides and architectural characteristic (patchily or continues meadows). Antibacterial assay of sponges tissue showed that only 7 sponge species has activity against targeting bacteria. The CA results also showed that sponge antibacterial activity was not correlated with seagrass species, with low bactericide and bacteriastatic activities. The implication of this result showed that sponges community can grow up at seagrass ecosystem eventhough their potential secondary metabolite activities is very low probably due to lack of stimulus mechanisms in the environment.

Co-Authors A. Sabdono ADI SANTOSO Agung Dhamar Syakti Agus Hardiyanto Agus Sabdono Agus Sabdono Agus Trianto Ali Ridlo Ambariyanto , Aninditia Sabdaningsih Antonius Budi Susanto Awang Surya Wiguna Awang Surya Wiguna, Awang Surya Bahry, Muhammad Syaifudien Bambang Yulianto Bayu Munandar Bintang Marhaeni Boedi Hendrarto Busaeri, Nundang Delianis Pringgenies Delianis Pringgenies Desy Wulan Triningsih Dietrich G Bengen Dietriech G. Bengen Dietriech Geoffrey Bengen Duhita Sinidhikaraning Kencana Dwi Haryo Ismunarti Elena Zocchi Endang Sri Lestari Endang Supriyantini Endang Supriyantini Fauziah Shahul Hamid Fera Nur Idawati Sahara Ferdy S. Rondonuwu Ferdy Semuel Rondonuwu Ferdy Semuel Rondonuwu Ferry Fredy Karwur Gede Iwan Setiabudi Gede Iwan Setiabudi Haeruddin Haeruddin Handung Nuryadi HANS- PETER GROSSART Hariyadi, Putut Hefni Effendi Hendi Perdian Yunianto Hendi Perdian Yunianto Herawati Sudoyo Hidetoshi Urakawa Hidetoshi Urakawal Ika Wulan Santi Ika Wulan Santi Iqna Kamila Abfa Irfan Dwidya Prijambada Isai Yusidharta Ita Riniatsih Ita Widowati JAKA WIDADA JOEDORO SOEDARSONO Johannes Hutabarat Kouichi Ohwada Kouichi Ohwadai Kumiko Kita-Tsukamoto Kumiko Kita-Tsukamotoz Leenawaty Limantara Lia Kusmita Lia Kusmita Mada Triandala Sibero Mada Triandala Sibero McCauley, Erin Meinhard Simon Meris Rahmawati Meutia Samira Ismet Miftahuddin M Khoeri Miftahuddin Majid Khoeri Misbakul Munir Muhammad Syaifudien Bahry Muhammad Zainuddin Muhammad Zainuddin Mujizat Kawaroe Mukti K. Diana Putri Mutiara, Frista Nestin E.W. Ningrum Ni Komang Tri Utami Norma Afiati Nuning Vita Hidayati Nurhayati Nurhayati Nurkhasanah, Wulan Nurmanita Rismaningsih Nurul Hiron, Nurul Nurul Ria Arlita Paulus Damar Bayu Murti Pinandoyo Pinandoyo Prastyo Abi Widyananto Putut Har Riyadi R. Riyanti Rachmat Afriyanto Richardus F Kaswadji Rismaningsih, Nurmanita Riyanti Riyanti . Riyanti Riyanti Riyanti Riyanti Riyanti, Rory Anthony Hutagalung Rr Citra Permata Rr Citra Permata Rr. Citra Permata Rr. Citra Permata Rudhi Pribadi Rudiger Stöhr S arjito S. Sulistiyani Saefuddin Aziz Sahala Hutabarat Sakti Imam Muchlisin Sakti Imam Muchlissin Sakti Imam Muchlissin Sarjito - Sekar Widyaningsih Septhy Kusuma Radjasa Seto Windarto Slamet B Prayitno slamet budi prayitno Slamet Budi Prayitno Sri Achadi Nugraheni Sri Sedjati Sri Yulina Wulandari Subagiyo Subagiyo Suharsono . Suhartono, Suhartono Supriharyono ,, Supriharyono Suyono ,, Suyono Suyono Suyono Tedi Septiadi Tonny Bachtiar Tonny Bachtiar TONNY BACHTIAR Tony Bachtiar Tony Bachtiar TORBEN MARTENS Tri Yuni Atmojo Tri Yuni Atmojo Uswatun Khasanah Vita Meylani Vivi Endar Herawati VIVI ENDAR HERAWATI Windarto, Seto Wiratno Wiratno Wismayanti, Gita Wittriansyah, Khoeruddin Wiwik Astuti Wiwik Astuti Wulan Nurkhasanah YS Darmanto Yustin Widyastuti

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