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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Rekayasa Proses JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Jurnal Ilmu Lingkungan Journal of Marine Research Microbiology Indonesia OSEATEK DIPOIPTEKS: Jurnal Ilmiah Mahasiswa Undip Jurnal Matematika & Sains Prosiding Indonesian Journal of Pharmaceutical Science and Technology Proceeding Seminar LPPM UMP Tahun 2014 Indonesian Journal of Biotechnology Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Marine Research in Indonesia Biosaintifika: Journal of Biology & Biology Education Jurnal Rekayasa Proses Indonesian Journal of Energy Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding University Research Colloquium Prosiding Konferensi Nasional Penelitian Matematika dan Pembelajarannya
Ocky Karna Radjasa
Badan Riset Dan Inovasi Nasional
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences Other

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Seleksi Isolat Bakteri Amilolitik dari Rhizosfer Canna edulis, Kerr. untuk Produksi Poli Hidroksi Alkanoat dari Limbah Cair Tapioka Nurhayati Nurhayati; Ocky Karna Radjasa; Irfan Dwidya Prijambada
Jurnal Rekayasa Proses Vol 11, No 2 (2017)
Publisher : Departemen Teknik Kimia Fakultas Teknik Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.8 KB) | DOI: 10.22146/jrekpros.33194

Abstract

Petrochemical-based plastic waste accumulated in landfills have been posing serious threat to the environment as this kind of plastics are non-biodegradable. Replacing petrochemical-based plastics with biodegradable plastics constitutes a challenging solution both in terms of mechanical design of the process and most importantly the availability of powerful local microorganism for the process. Therefore, the current study was searching for appropriate local microorganisms for poly hydroxyl alkanoate (PHA) production from starch waste, which was considered as one of cheap carbon sources. Waste water of cassava industry is a good resource of such starch waste water. The microbes were isolated from Canna edulis, Kerr. rhizosphere from Cangkringan.  The expected isolates were the bacteria enable the coupling of carbon catabolic pathways with PHA anabolic pathways. It was found that ten isolates were able to use waste water of cassava flour industry as carbon source. The PHA quantitative analysis by spectrophotometer showed that the isolate of Bacillus sp. C8 produced the highest PHA of 2,095 g/L. Further FTIR analysis showed specific bands near 1363,67 cm-1, 1641,42 cm-1, 2929,87 cm-1, 3408,22 cm-1 wavelengths which revealed the presence of CH3, ester carbonyl group (C=O), C-H and terminal OH group of PHA. ABSTRAKAkumulasi sampah plastik berbasis petrokimia di tempat pembuangan sampah mengganggu lingkungan karena plastik sifatnya tidak mudah didegradasi secara biologi dan sangat tahan di lingkungan. Penggantian plastik yang berasal dari bahan petrokimia dengan bahan plastik yang mudah terdegradasi secara biologi merupakan tantangan tersendiri, baik dari sisi perancangan proses maupun ketersediaan mikrobia lokal yang sesuai untuk proses tersebut. Oleh karena itu, penelitian ini dilakukan sebagai upaya untuk mendapatkan bakteri lokal penghasil PHA yang mampu mengkonsumsi substrat berupa pati. Substrat pati dipilih karena ketersediaan limbat industri tapioka sebagai bahan baku potensial dan murah untuk produksi PHA. Bakteri amilolitik untuk produksi PHA telah berhasil diisolasi dari rhizosfer Canna edulis, Kerr. di Cangkringan, Sleman, Yogyakarta, Indonesia. Bakteri yang diisolasi merupakan bakteri dengan kemampuan memproduksi PHA dan memiliki kemampuan melakukan rangkaian reaksi pada limbah cair industri tapioka dan rangkaian reaksi pembentukan PHA. Telah berhasil didapatkan 10 bakteri yang memiliki aktivitas amilolitik dan dapat menghasilkan PHA  menggunakan limbah cair industri tapioka. Analisis kuantitatif PHA menggunakan spektrofotometer menunjukkan bahwa isolat Bacillus sp. C8 menghasilkan PHA paling tinggi sebesar 2,095 mg/mL. Analisis hasil metabolism isolat C8 menggunakan FTIR memperlihatkan puncak spesifik 1363,67 cm-1, 1641,42 cm-1, 2929,87 cm-1, 3408,22 cm-1 adalah verifikasi adanya CH3, C=O, C-H dan OH dari PHA.
ANTIFOULING ACTIVITY OF BACTERIA ASSOCIATED WITH SOFT CORAL Sarcophyton sp. AGAINST MARINE BIOFILM-FORMING BACTERIA Agus Sabdono; Ocky Karna Radjasa
JOURNAL OF COASTAL DEVELOPMENT Vol 10, No 1 (2006): Volume 10, Number 1, Year 2006
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Marine bacteria associated with soft coral Sarcophyton sp collected from vicinity of Peucang island, Ujung Kulon, West Java, were successfully screened for antifouling activity against marine biofilm-forming bacteria isolated from the surrounding colonies of Sarcophyton sp. Six bacterial isolates were found to inhibit the growth of at least one of 7 biofilm-forming isolates.  The most active strain USP3.37 was identified as Pelagiobacter variabilis by using 16S rDNA gene sequence analysis. Similarly, the active strains USP3.3, USP8.43, USP3.12, USP3.16 and USP8.6 were identified as Arthrobacter nicotianae,  Shewanella alga, Pseudomonas synxantha, Pseudomonas falgida, Pseudovibrio denitrificans and Bacillus aquamaris, respectively. USP3.37 strain was found to amplify gene fragments of non-ribosomal peptide synthetase (NRPS).  This raises the possibility the use of softcoral bacteria as the source of antibacterial compounds for controlling the antifouling in the sea. Therefore,  this bacterium would be better to select eco-friendly antifouling compounds than the other antibacterial activities.
PHYLOGENETIC CHARACTERIZATION OF PSYCHROPHILIC ° BACTERIA ISOLATED FROM DEEP-SEA WATER OF THE NANKAI TROUGH Ocky Karna Radjasa; Kumiko Kita-Tsukamotoz; Hidetoshi Urakawal; Kouichi Ohwadai
JOURNAL OF COASTAL DEVELOPMENT Vol 4, No 3 (2001): Volume 4, Number 3, Year 2001
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

A screening among 4"C-culturable isolates obtainedfom dcep~sea water of Nankai Trough from a depth of approximately 4,000 m resulted in a total of 5 psychrophilic bacteria showing no growth at 20° C. Phylogenetic characterization based on l6S ribosomal DNA sequence analysis showed that the isolates belonged to the genus Colwellia, Moritella, and CNPT-3, which could be assigned to Psychromonas.
ANTIBACTERIAL ACTIVITY OF BACTERIAL SYMBIONT OF SOFT CORAL Lobophytum sp. AGAINST MDR BACTERIA Escherichia coli and Staphyllococcus aureus Paulus Damar Bayu Murti; Ocky Karna Radjasa
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 3 (2012): Volume 15, Number 3, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

The improper and uncontrolled uses of antibiotics againts pathogenic bacteria have resulted in the occurrence of Multi Drug Resistant (MDR) strains. There is now an urgency to find alternative antibiotics to combat the MDR strains especially Escherichia coli and Staphyllococcus aureus. Soft coral associated microorganisms are among the most interesting and promising marine natural product sources which produce polyketide and non ribosomal peptide products with various biological activities. In this study, marine bacteria were isolated from soft coral Lobophytum sp. collected from North Java Sea, and were screened for antibacterial activity against MDR strains. One out of 13 bacterial isolates were succesfully screened and were found to be active against both MDR strains, in which isolate LBTGA2 was active against resistant strains E. coli and against resistant S. aureus, respectively. The active isolate also amplified PKS (Polyketide Synthases) gene fragments necessary for the biosynthesis  of polyketides. The molecular identification based on partial 16S DNA nukleotide sequences indicates that the active isolate was closely related to Paenibacillus campinasensis.
THE GROWTH INHIBITION OF MARINE BIOFILM-FORMING BACTERIA BY THE CRUDE EXTRACT OF SOFT CORAL Sinularia sp. Ocky Karna Radjasa; A. Sabdono; Suharsono .
JOURNAL OF COASTAL DEVELOPMENT Vol 2, No 2 (1999): Volume 2, Number 2, Year 1999
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Marine biofouling has been recognized as a big problem faced by marine technology, and has caused huge economic losses to marine industries. Until recently, marine biofouling has been controlled by the use of metal-based coating which has become another problem because of their negative impacts on marine environments. Soft corals have been known to produce secondary metabolites, some of which may inhibit the fouling processes. The objective of this research was to examine the antimicrobial properties of soft coral Sinularia sp against marine biofilm-forming bacteria The results showed that the soft coral tissues of Sinularia sp. had the antibacterial potency. The crude extracts of Sinularia sp affected significantly on the growth of bacteria tested. The optimal concentration of crude extracts needed to inhibit the growth of bacteria was 150 µg/ml. There were no significantly different among bacteria isolated from fiber, wood and iron steel on diameter of inhibitory zone of the bacterial growth. It is concluded thet the search of bioactive substances produced by soft corals is great possibility to find alternatives for metal-based coatings. Yet, a series of researchs must be undertaken in order to find the secondary metabolites which may be used as antifoulant.
ANTIBACTERIAL ACTIVITIES OF BACTERIAL SYMBIONTS OF SOFT CORAL Sinularia sp. AGAINST TUBERCULOSIS BACTERIA S. Sulistiyani; Sri Achadi Nugraheni; Ocky Karna Radjasa; Agus Sabdono; Miftahuddin Majid Khoeri
JOURNAL OF COASTAL DEVELOPMENT Vol 14, No 1 (2010): Volume 14, Number 1, Year 2010
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Tuberculosis (TB) is caused by Mycobacterium tuberculosis. Although TB is a curable disease, it continues tobe one of the most important infectious causes of death worldwide. Indonesia ranks 3rd on the list of TB highburden countries in the world with 86,000 cases deaths and the Multi Drug Resistant Tuberculosis (MDR TB)estimated cases in Indonesia is 10,000. This research was aimed to isolate and characterize of soft coralSinularia sp.-associated bacteria having antibacterial activity against Tuberculosis bacteria. There were109 isolates collected from Sinularia sp. Two isolates from Sinularia sp.-associated bacteria, SC4TGZ3 andSC4TGZ4 were successfully screened for antibacterial against Tuberculosis bacteria. SC4TGZ3 was found toinhibit the growth of MDR TB strain HE, MDR TB strain SR and H37Rv. Whereas, SC4TGZ4 was found toinhibit the growth of MDR TB strain HE. Based on PCR amplification 16S rDNA softcoral bacateria wereidentified as follows: SC4TGZ3 was closely related to Pseudovibrio sp. and SC4TGZ4 was closely related toAlpha proteobacterium sp.
SCREENING OF BACTERIAL SYMBIONTS OF SEAGRASS Enhalus sp. AGAINST BIOFILM-FORMING BACTERIA Bintang Marhaeni; Ocky Karna Radjasa; Dietrich G Bengen; Richardus F Kaswadji
JOURNAL OF COASTAL DEVELOPMENT Vol 13, No 2 (2010): Volume 13, number 2, Year 2010
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Seagrasses have been known to produce secondary metabolites that have important ecological roles, including preventing from pathogen infections and fouling organisms. A research aimed at screening the potential of bacterial symbionts of seagrass Enhalus sp. was performed. Bacterial symbionts including endophytes and epiphytes were isolated from the seagrass, and marine biofilm-forming bacteria were isolated from the fiber and wooden panels from the surrounding colonies. A total of 17 epiphyte and 6 endophyte isolates were obtained, however more biological activity was found among endophytes (100%) compared to epiphytes (47%) against biofilm-forming bacteria. In addition, bacterial endophytes inhibited more biofilm-forming bacteria than epiphytes. Interestingly more isolates were obtained from rough surfaces both from fiber and wooden panels than smoothe surfaces. Bacterial symbionts of seagrass Enhalus sp., in particular its endophytes show potential source as  natural marine antifoulants.       
CHARACTERIZATION AND IDENTIFICATION OF STRAIN KM221, A NOVEL MCPA HERBICIDE-DEGRADING BACTERIUM ISOLATED FROM CORAL SURFACE, MENJANGAN KECIL ISLAND, KARIMUNJAWA Agus Sabdono; Ocky Karna Radjasa; Joedoro Soedarsono
JOURNAL OF COASTAL DEVELOPMENT Vol 6, No 3 (2003): Volume 6, Number 3, Year 2003
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

In this study, bacterial strain KM221 was isolated from coral   tissue in Menjangan Kecil Island, Karimunjawa, Indonesia. This strain is  facultative anaerobic  with MCPA (2-methyl-4-chlorophenoxy acetic acid) serving as the only known energy sources.  Microscopy  of isolate revealed that strain KM221 is gram-positive, catalase-positive,  rod, spore-forming bacterium, motile,  opaque, hair-like outgrowth and unpigmented colonies. The bacterium could not be identified on the basis of its carbon-source-utilization pattern, but a partial sequencing of the 16S rDNA analysis suggest that this strain is closely related to Bacillus iodinum.The ability to degrade MCPA herbicide was examined qualitatively in EMBA indicator medium.  This bacterium grew exponentially with MCPA as  the sole source of energy and carbon. The maximum growth rate (m max)  and the  saturated concentration on MCPA (Cs) were determined to be  0.8024 h-1 and  5.10 mg/l MCPA, respectively.
ANTIBACTERIAL ACTIVITY OF A PIGMENT PRODUCING-BACTERIUM ASSOCIATD WITH Halimeda sp. FROM ELAND-LOCKED MARINE LAKE KAKABAN, INDONESIA Ocky Karna Radjasa; Leenawaty Limantara; Agus Sabdono
JOURNAL OF COASTAL DEVELOPMENT Vol 12, No 2 (2009): Volume 12, Number 2, Year 2009
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

A pigment producing-bacterium associated with green alga Halimeda sp., was successfully isolated from a land-locked marine lake Kakaban, East Borneo, Indonesia and screened for an antibacterial activity against pathogenic Staphylococcus aureus. The bacterium was identified as Pseudoalteromonas piscicida based on its 16S rDNA and was found to produce xanthophyll pigments and to amplify gene fragments of Non-ribosomal peptide synthetase (NRPS).
CHARACTERIZATION OF CAROTENOID PIGMENTS FROM BACTERIAL SYMBIONTS OF SEAGRASS Thalassia hemprichii Sri Achadi Nugraheni; Miftahuddin M Khoeri; Lia Kusmita; Yustin Widyastuti; Ocky Karna Radjasa
JOURNAL OF COASTAL DEVELOPMENT Vol 14, No 1 (2010): Volume 14, Number 1, Year 2010
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Carotenoids are pigments that can be used in various applications including cosmetics and precursor ofvitamins A. Carotenoids are mostly found in higher plant leaves, fruit, and bacteria. Marine bacteriaassociated with seagrass Thalassia hemprichii collected from Menjangan Kecil Waters, KarimunjawaIslands were screened to produce the pigment and has allowed the use of these microrganism as anenvironmental friendly alternative source of new natural pigment. The isolation of bacterial symbionts onZobell 2216E medium from seagrass Thalassia hemprichii resulted in 20 isolates of which 8 bacterialsymbionts have produced pigments but only one bacterium positively synthesize carotenoids. Initial analysiswith atomic absorption spectrophotometric method revealed that the wave lengt of bacterial pigment were inthe range of 300-600 nm, which are categorized that within the group of carotenoid pigments. From theresults of molecular identification by 16S rDNA method, it was shown that bacterium TH8 was closelyrelated to Bacillus licheniformis with 98% homology value.
Co-Authors A. Sabdono ADI SANTOSO Agung Dhamar Syakti Agus Hardiyanto Agus Sabdono Agus Sabdono Agus Trianto Ali Ridlo Ambariyanto , Aninditia Sabdaningsih Antonius Budi Susanto Awang Surya Wiguna Awang Surya Wiguna, Awang Surya Bahry, Muhammad Syaifudien Bambang Yulianto Bayu Munandar Bintang Marhaeni Boedi Hendrarto Busaeri, Nundang Delianis Pringgenies Delianis Pringgenies Desy Wulan Triningsih Dietrich G Bengen Dietriech G. Bengen Dietriech Geoffrey Bengen Duhita Sinidhikaraning Kencana Dwi Haryo Ismunarti Elena Zocchi Endang Sri Lestari Endang Supriyantini Endang Supriyantini Fauziah Shahul Hamid Fera Nur Idawati Sahara Ferdy S. Rondonuwu Ferdy Semuel Rondonuwu Ferdy Semuel Rondonuwu Ferry Fredy Karwur Gede Iwan Setiabudi Gede Iwan Setiabudi Haeruddin Haeruddin Handung Nuryadi HANS- PETER GROSSART Hariyadi, Putut Hefni Effendi Hendi Perdian Yunianto Hendi Perdian Yunianto Herawati Sudoyo Hidetoshi Urakawa Hidetoshi Urakawal Ika Wulan Santi Ika Wulan Santi Iqna Kamila Abfa Irfan Dwidya Prijambada Isai Yusidharta Ita Riniatsih Ita Widowati JAKA WIDADA JOEDORO SOEDARSONO Johannes Hutabarat Kouichi Ohwada Kouichi Ohwadai Kumiko Kita-Tsukamoto Kumiko Kita-Tsukamotoz Leenawaty Limantara Lia Kusmita Lia Kusmita Mada Triandala Sibero Mada Triandala Sibero McCauley, Erin Meinhard Simon Meris Rahmawati Meutia Samira Ismet Miftahuddin M Khoeri Miftahuddin Majid Khoeri Misbakul Munir Muhammad Syaifudien Bahry Muhammad Zainuddin Muhammad Zainuddin Mujizat Kawaroe Mukti K. Diana Putri Mutiara, Frista Nestin E.W. Ningrum Ni Komang Tri Utami Norma Afiati Nuning Vita Hidayati Nurhayati Nurhayati Nurkhasanah, Wulan Nurmanita Rismaningsih Nurul Hiron, Nurul Nurul Ria Arlita Paulus Damar Bayu Murti Pinandoyo Pinandoyo Prastyo Abi Widyananto Putut Har Riyadi R. Riyanti Rachmat Afriyanto Richardus F Kaswadji Rismaningsih, Nurmanita Riyanti Riyanti . Riyanti Riyanti Riyanti Riyanti Riyanti, Rory Anthony Hutagalung Rr Citra Permata Rr Citra Permata Rr. Citra Permata Rr. Citra Permata Rudhi Pribadi Rudiger Stöhr S arjito S. Sulistiyani Saefuddin Aziz Sahala Hutabarat Sakti Imam Muchlisin Sakti Imam Muchlissin Sakti Imam Muchlissin Sarjito - Sekar Widyaningsih Septhy Kusuma Radjasa Seto Windarto Slamet B Prayitno slamet budi prayitno Slamet Budi Prayitno Sri Achadi Nugraheni Sri Sedjati Sri Yulina Wulandari Subagiyo Subagiyo Suharsono . Suhartono, Suhartono Supriharyono ,, Supriharyono Suyono ,, Suyono Suyono Suyono Tedi Septiadi Tonny Bachtiar TONNY BACHTIAR Tonny Bachtiar Tony Bachtiar Tony Bachtiar TORBEN MARTENS Tri Yuni Atmojo Tri Yuni Atmojo Uswatun Khasanah Vita Meylani Vivi Endar Herawati VIVI ENDAR HERAWATI Windarto, Seto Wiratno Wiratno Wismayanti, Gita Wittriansyah, Khoeruddin Wiwik Astuti Wiwik Astuti Wulan Nurkhasanah YS Darmanto Yustin Widyastuti