Article Per Year (5 Year)
p-Index From 2020 - 2025
1.756
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Rekayasa Proses JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Jurnal Ilmu Lingkungan Journal of Marine Research Microbiology Indonesia OSEATEK DIPOIPTEKS: Jurnal Ilmiah Mahasiswa Undip Jurnal Matematika & Sains Prosiding Indonesian Journal of Pharmaceutical Science and Technology Proceeding Seminar LPPM UMP Tahun 2014 Indonesian Journal of Biotechnology Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Marine Research in Indonesia Biosaintifika: Journal of Biology & Biology Education Jurnal Rekayasa Proses Indonesian Journal of Energy Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding University Research Colloquium Prosiding Konferensi Nasional Penelitian Matematika dan Pembelajarannya
Ocky Karna Radjasa
Badan Riset Dan Inovasi Nasional
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences Other

Published : 81 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 81 Documents
Search

 3   4   5   6   7 
GROWTH INHIBITION OF MEDICALLY ANTIBIOTIC RESISTANT BACTERIA BY SPONGE-ASSOCIATED BACTERIA Ocky Karna Radjasa
JOURNAL OF COASTAL DEVELOPMENT Vol 11, No 2 (2008): Volume 11, Number 2, Year 2008
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.192 KB)

Abstract

The improper and uncontrolled uses of antibiotics against pathogenic bacteria have resulted in theoccurrence of Multi Drugs Resistant bacteria. There is now an urgency to find alternative antibioticsto combat these bacteria. The metabolites from microorganisms are a rapidly growing field, due, atleast in part, to the suspicion that a number of metabolites obtained from algae and invertebrates maybe produced by associated microorganisms. Therefore, there is a shift in the search for secondarymetabolites from terrestrial to marine environment. Sponge-associated microorganisms are among ofthe most interesting and promising marine natural product sources, which produce polyketide and nonribosomal peptide products with various biological activities. In this study, marine bacteria wereisolated from sponge Haliclona sp. collected from North Java Sea, and were screened for antibacterialactivity against MDR strains. One out of 32 bacterial isolates were successfully screened and werefound to be active against MDR strains, strain Escherichia coli and strain Proteus sp., respectively.These active isolates were also capable of amplifying NRPS gene fragments necessary for thebiosynthesis of non ribosomal peptides. The identification results revealed that the active isolates areArthrobacter sp.
APPLICATION OF REPETITIVE SEQUENCE-BASED PCR ON THE RICHNESS OF VIBRIO ON THE TIGER SHRIMP (Penaeus monodon Fab.) S arjito; Nestin E.W. Ningrum; Ocky Karna Radjasa; Slamet Budi Prayitno
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 3 (2012): Volume 15, Number 3, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186 KB)

Abstract

A molecular-based study was conducted to estimate the richness of the Vibrio on  tiger shrimp (Penaeus monodon Fab.) from brackish water pond of Mororejo, Kendal. Tiger shrimps were collected from the extensive brackish water pond and a total of 22 isolates were obtained from hepatopancreas and telson of tiger shrimp. Based on the repetitive sequence-based polymerase chain reaction (rep-PCR), it was found that two groups of Vibrio. To investige the efectiveness of rep-PCR in estimating the richness of Vibrio on tiger shrimps, three isolates  (JTW 01, JTW 03 and JTW 06) were chosen for further investigation. On the basis of sequence analysis, the result showed  that the JTW 01, JTW 03 and JTW 06 were closely related to Vibrio sp. Absa7 clone 423.1, Vibrio splendidus and Vibrio splendidus, respectively.  The result proved that two assosiated of Vibrio on tiger shrimp were Vibrio sp. Absa7 clone 423.1 and Vibrio splendidus. Therefore  the present study highlights the effectiveness of rep-PCR in rapid grouping and estimating the richness of Vibrio on  tiger shrimp.
PHYLOGENETIC RELATIONSHIP OF BAROPHILIC BACTERIA FROM NORTHWESTERN PACIFIC OCEAN Ocky Karna Radjasa
JOURNAL OF COASTAL DEVELOPMENT Vol 5, No 1 (2001): Volume 5, Number 1, Year 2001
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.524 KB)

Abstract

In order to elucidate the role of hydrostatic pressure on the distribution of marine microorganisms, deep-sea water samples from north-western Pacific Ocean were collected and used as the source of barophilic bacteria. One isolate, JTW-863 was obtained from a depth of 6000 m, while two isolates, MTW-1 and MTW-13, were obtained from a depth of 10,500 m. Phylogenetic analysis based on PCR-amplified 16S rDNA revealed that JTW-863 belonged to Moritella, while MTW-1 was mostly related to Shewanella. On the other hand, MTW-13 was affiliated with the uncultured clone NB1-d from deep environment.
DEEP- SEA BACTERIA AND THEIR BIOTECHNOLOGICAL POTENTIALS Ocky Karna radjasa
JOURNAL OF COASTAL DEVELOPMENT Vol 7, No 3 (2004): Volume 7, Number 3, Year 2004
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.622 KB)

Abstract

Deep-sea environment characterized by its low temperature, high hydrostatic pressure, limited nutrient contents, and constant darkness has been a hostile environment for most marine microorganisms. Therefore it is a home for low temperature and high pressure-loving bacteria believed to functionally over shallow water intruders. Deep-sea has been regarded as rainforests in terms of their microbial diversity. Thus, the deep-sea should commend itself to microbiologists and biotechnologists alike as source of novel microorganisms and exploitable properties. Near Indonesia several deep-sea trenches exist with diverse environmental conditions. However, up to now there has been no effort to study the microbial communities of those deep-sea environments by Indonesian scientists. This is mainly because of the lack of knowledge in the field of deep-sea microbiology in Indonesia.
RAPID GROUPING OF MARINE PSYCHROTROPIC BACTERIA USING RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF PCR-AMPLIFIED 16S Rdna Ocky Karna Radjasa; Hidetoshi Urakawa; Kumiko Kita-Tsukamoto; Kouichi Ohwada
JOURNAL OF COASTAL DEVELOPMENT Vol 3, No 1 (1999): Volume 3 Number 1 Year 1999
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2079.219 KB)

Abstract

Grouping of 78 psychrotrophic bacteria isolated from surface and deep-sea waters of the north-western Pacific Ocean was carried out by RFLP analysis on the basis of PCR-amplified 16S rDNA. Six RFLP patterns from surface water and eight RFLP patterns from deep-sea water were obtained by HhaI digestion. F pattern was only found in the surface water, while g, h, and I patterns exclusively exited in the deep-sea water. It was clearly shown that RFLP patterns of HhaI digestion were clear and distinguishable. This method is very simple and promises a rapid tool to group numerous isolated from natural environments.
SCREENING OF SECONDARY METABOLITE-PRODUCING BACTERIA ASSOCIATED WITH CORALS USING 16S rDNA-BASED APPROACH Ocky Karna Radjasa; Agus Sabdono
JOURNAL OF COASTAL DEVELOPMENT Vol 7, No 1 (2003): Volume 7, Number 1, Year 2003
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.213 KB)

Abstract

A total of 42 coral-associated bacteria were screened for their ability to produce secondary metabolites by using 16S rDNA-based approach. A specific oligonucleotides primer was used to amplify DNA non-ribosomal peptide synthetase (NRPS). Five strains were found to carry the NRPS gene and successfully inhibit the growth of other coral-associated and pathogenic bacteria. The phylogenetic characterization revealed that four strains belonged to currently known species Pseudoalteromonas luteoviolacea, Vibrio tubiashi, Vibrio carchiariae and Bacillus iodinum. On the other hand, one strain was apparently closely related with an unknown group of the gamma Proteobacteria, which was only recognized as uncultured clone 141H2.
PHYLOGENETIC DIVERSITY OF SECONDARY METABOLITE PRODUCING-BACTERIA ASSOCIATED WITH SPONGES FROM BANDENGAN WATERS, JEPARA Ocky Karna Radjasa; Agus Sabdono
JOURNAL OF COASTAL DEVELOPMENT Vol 10, No 1 (2006): Volume 10, Number 1, Year 2006
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.415 KB)

Abstract

A total of 13 bacterial isolates associated with sponges collected from Bandengan water, Jepara, North Java Sea, Indonesia, was successfully screened for antibacterial activity against pathogenic bacteria Vibrio parahaemolyticus, Aeromonas hydrophila  and Staphylococcus aureus. Active bacterial isolates were rapidly grouped by using rep-PCR and a dendrogram was constructed. Six isolates were further selected based on the constructed dendrogram for subsequent DNA sequencings, resulted in the richness of secondary metabolite-producing bacteria associated with sponges from Bandengan having closest similarity to Pseudoalteromonas, Brachybacterium, Vibrio, alpha proteobacterium and uncultured bacterium clones.
ANTIBACTERIAL ACTIVITY OF SPONGE ASSOCIATED-BACTERIA ISOLATED FROM NORTH JAVA SEA Ocky Karna Radjasa
JOURNAL OF COASTAL DEVELOPMENT Vol 10, No 3 (2007): Volume 10, Number 3, Year 2007
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.25 KB)

Abstract

A total of 90 bacterial isolates were obtained from association with sponges collected from Bandengan water, Jepara and Karimun island of Karimunjawa islands in the north java sea. Antibacterial screening by using a pathogenic Escherichia coli indicated that two isolates BSP.12 and MKSP.5 inhihited the growth of tested strain. Molecular identification based on 16S rDNA approach revealed that isolate BSP.12 was closely related to Vibrio harveyi with a homology of 100% and isolate MKSP.5 showed highest similarity to Brachybacterium rhamnosum (99%), respectively.A PCR-based approach by using specific primers targeting the occurrence of gene fragments necessary for the biosynthesis of major natural products, namely Non-ribosomal peptide synthetases (NRPS) and Polyketide synthases (PKS) was applied to estimate the genetic potential of these active strains. Both isolates were capable of amplifying the NRPS gene fragments but not the PKS gene fragments.
DIVERSITY OF CULTURABLE BACTERIAL COMMUNITY ASSOCIATED WITH THE CORAL Galaxea fascicularis FROM UJUNG KULON, INDONESIA Agus Sabdono; Ocky Karna Radjasa; Rudiger Stöhr; Elena Zocchi
JOURNAL OF COASTAL DEVELOPMENT Vol 9, No 1 (2005): Volume 9, Number 1, Year 2005
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.913 KB)

Abstract

Coral reefs are the most diverse marine ecosystems; however, little is known about their microbialdiversity in these ecosystems. The present study is aimed at investigating the general insights into thediversity of the bacterial community associated with the coral Galaxea fascicularis. A culture collectionof 45 bacteria associated with coral G. fascicularis from Ujung Kulon, Indonesia was established byplating on Zobell’s 2214E. Isolates were screened by means of RLFP and sequencing of representative16S rDNAs. Using the restriction enzyme HaeIII, isolates were classified into 8 pattern group. Thesequence results indicated that a high diversity of bacterial phylotypes was present within the coralGalaxea fascicularis. In general, there are three major groups of bacteria: (i) members of the division Firmicutes , (ii) Actinobacteria, and (iii) γ-proteobacteria. Phylogenetic data on microbial communitycomposition in coral G. fascicularis will help in the rational selection of culture conditions to improvethe diversity of bacteria and the knowledge on the physiological, biochemical, genetic, and molecularproperties of coral bacteria.
BIOPROSPECTING OF BACTERIAL SYMBIONT OF Tunicate Didemnum molle FROM SAMBANGAN, KARIMUNJAWA ISLANDS Miftahuddin Majid Khoeri; Ocky Karna Radjasa; Agus Sabdono; Herawati Sudoyo
JOURNAL OF COASTAL DEVELOPMENT Vol 14, No 3 (2011): Volume 14, Number 3, Year 2011
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.384 KB)

Abstract

Coral reef is a productive ecosystem with high biodiversity in the sea and being targeted to find a useful bioactive compound. However, the serious problem in development of bioactive compounds from marine invertebrate is the supply problem, because to get a small amounts of active compounds a massive numbers of sea organisms are needed. Tunicate is an animal in coral reef ecosystem that produces many bioactive compounds with pharmacological activities, such as, antibacterial, antitumor, and anticancer compounds. It has been reported that bacterial symbionts of coral reef invertebrates may synthesize the same compounds as the host. The purposes of this research are to isolate and to identify microbes which have antibacterial activity against MDR bacteria based PCR 16S rRNA and to detect the existence of  PKS and NRPS biosynthetic gene fragments from tunicate bacteria of Didemnum molle. Out of 15 bacterial isolates, one isolate showed antibacterial potential against Escherichia coli and Staphylococcus sp. Molecular identification result showed that TS2A5 bacterium has a homology of 99 % with Virgibacillus sp. strain GSP17 16S ribosomal RNA gene. This isolate was also capable of amplifying  NRPS gene fragment.
Co-Authors A. Sabdono ADI SANTOSO Agung Dhamar Syakti Agus Hardiyanto Agus Sabdono Agus Sabdono Agus Trianto Ali Ridlo Ambariyanto , Aninditia Sabdaningsih Antonius Budi Susanto Awang Surya Wiguna Awang Surya Wiguna, Awang Surya Bahry, Muhammad Syaifudien Bambang Yulianto Bayu Munandar Bintang Marhaeni Boedi Hendrarto Busaeri, Nundang Delianis Pringgenies Delianis Pringgenies Desy Wulan Triningsih Dietrich G Bengen Dietriech G. Bengen Dietriech Geoffrey Bengen Duhita Sinidhikaraning Kencana Dwi Haryo Ismunarti Elena Zocchi Endang Sri Lestari Endang Supriyantini Endang Supriyantini Fauziah Shahul Hamid Fera Nur Idawati Sahara Ferdy S. Rondonuwu Ferdy Semuel Rondonuwu Ferdy Semuel Rondonuwu Ferry Fredy Karwur Gede Iwan Setiabudi Gede Iwan Setiabudi Haeruddin Haeruddin Handung Nuryadi HANS- PETER GROSSART Hariyadi, Putut Hefni Effendi Hendi Perdian Yunianto Hendi Perdian Yunianto Herawati Sudoyo Hidetoshi Urakawa Hidetoshi Urakawal Ika Wulan Santi Ika Wulan Santi Iqna Kamila Abfa Irfan Dwidya Prijambada Isai Yusidharta Ita Riniatsih Ita Widowati JAKA WIDADA JOEDORO SOEDARSONO Johannes Hutabarat Kouichi Ohwada Kouichi Ohwadai Kumiko Kita-Tsukamoto Kumiko Kita-Tsukamotoz Leenawaty Limantara Lia Kusmita Lia Kusmita Mada Triandala Sibero Mada Triandala Sibero McCauley, Erin Meinhard Simon Meris Rahmawati Meutia Samira Ismet Miftahuddin M Khoeri Miftahuddin Majid Khoeri Misbakul Munir Muhammad Syaifudien Bahry Muhammad Zainuddin Muhammad Zainuddin Mujizat Kawaroe Mukti K. Diana Putri Mutiara, Frista Nestin E.W. Ningrum Ni Komang Tri Utami Norma Afiati Nuning Vita Hidayati Nurhayati Nurhayati Nurkhasanah, Wulan Nurmanita Rismaningsih Nurul Hiron, Nurul Nurul Ria Arlita Paulus Damar Bayu Murti Pinandoyo Pinandoyo Prastyo Abi Widyananto Putut Har Riyadi R. Riyanti Rachmat Afriyanto Richardus F Kaswadji Rismaningsih, Nurmanita Riyanti Riyanti . Riyanti Riyanti Riyanti Riyanti Riyanti, Rory Anthony Hutagalung Rr Citra Permata Rr Citra Permata Rr. Citra Permata Rr. Citra Permata Rudhi Pribadi Rudiger Stöhr S arjito S. Sulistiyani Saefuddin Aziz Sahala Hutabarat Sakti Imam Muchlisin Sakti Imam Muchlissin Sakti Imam Muchlissin Sarjito - Sekar Widyaningsih Septhy Kusuma Radjasa Seto Windarto Slamet B Prayitno slamet budi prayitno Slamet Budi Prayitno Sri Achadi Nugraheni Sri Sedjati Sri Yulina Wulandari Subagiyo Subagiyo Suharsono . Suhartono, Suhartono Supriharyono ,, Supriharyono Suyono ,, Suyono Suyono Suyono Tedi Septiadi TONNY BACHTIAR Tonny Bachtiar Tonny Bachtiar Tony Bachtiar Tony Bachtiar TORBEN MARTENS Tri Yuni Atmojo Tri Yuni Atmojo Uswatun Khasanah Vita Meylani Vivi Endar Herawati VIVI ENDAR HERAWATI Windarto, Seto Wiratno Wiratno Wismayanti, Gita Wittriansyah, Khoeruddin Wiwik Astuti Wiwik Astuti Wulan Nurkhasanah YS Darmanto Yustin Widyastuti