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Hiola, Faramita
Universitas Negeri Gorontalo
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ANTIOXIDANT ACTIVITY EVALUATION OF CATFISH (CLARIAS SP.) COLLAGEN EXTRACT USING DPPH AND ABTS METHODS Hiola, Faramita; Zuriati Uno, Wiwit; Sy. Pakaya, Mahdalena; Hutuba, Ariani
Jurnal Multidisipliner Kapalamada Vol. 4 No. 04 (2025): JURNAL MULTIDISIPLINER KAPALAMADA
Publisher : Pusat Studi Ekonomi, Publikasi Ilmiah dan Pengembangan SDM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62668/kapalamada.v4i04.1964

Abstract

This study aimed to evaluate the antioxidant activity of collagen extracted from catfish (Clarias sp.) using DPPH and ABTS assays. The research procedures included sample preparation, collagen extraction using an acid-based method, and antioxidant analysis at concentrations ranging from 20–100 ppm. The degree of hydrolysis (DH) was measured to determine the effectiveness of Alkalase in producing bioactive peptides. The results showed that higher enzyme concentrations led to increased DH, with the highest value of 62.42% obtained at 3% Alkalase. Antioxidant activity increased with sample concentration, with maximum inhibition values of 78.57% (DPPH) and 85.00% (ABTS) at 100 ppm. The ABTS assay exhibited higher sensitivity than DPPH. These findings indicate that catfish collagen has strong potential as a safe and natural antioxidant source suitable for further development in food, pharmaceutical, and cosmetic applications.
Co-Authors A. Mu’thi Andy Suryadi Akuba, Juliyanti Andy Suryadi, A. Mu’thi Anggai, Rifka Anggraini Ariani H. Hutuba Ayu Maloho, Sity Nur Rahayu Maloho Dewa Ayu Puspita Divia Handayani Mudjidu Dr. Apt. Hamsidar Hasan S.Si.,M.Si Endah Nurrohwinta Djuwarno Fika Nuzul Ramadhani Gani, Athira Sri Wahyuni Goni, Latifa Gunawan, Selvi Ayu Setiaarini Halada, Ichlasul Oktofandi Hamsidar Hasan Hamzah, Alifiah Ramawaty Hasrita Samudi Hutuba, Ariani Hutuba, Ariani H Iman, Devansyah Ishak Isa Ismail, Delvita Ismail, Ulfatul Magfirah M. Julianty Akuba Kahar, Putri Kartika Adhilia Katili, Ayub H. Lahay, Astiara Lia Amalia LIA AMALIA Liputo, Kartika Vany Madania, Madania Madania, Madania Mahdalena Sy Pakaya mahdalena sy. pakaya Makkulawu, Andi Mangulu, Anindia Mohamad Aprianto Paneo Muhammad Taupik Muhammad Taupik Nanang R. Paramata Nanang Roswita Paramata Nur Ain Thomas Nur Rasdianah Pakaya, Meri Isriani Papeo, Dizky Ramadani Putri Paputungan, Jihan Utari Puluhulawa, Lisa Efriani Putri Ayuningtias Mahdang Putri Ayuningtias Mahdang Rannu, Jumriani Robert Tungadi Salamanya, Ahmad Hayun Samsi, Khairun nisha Selong, Siti Ruwaida D. Sri Nur'ain E. Ahmad Suleman, Ahmad Teti Sutriyati Tuloli Widy Susanti Abdulkadir Widy Susanti Abdulkadir Widy Susanti Abdulkadir Yasir Mokodompis Zuriati Uno, Wiwit