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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Penyuluhan Jurnal Manajemen dan Agribisnis Media Veteriner Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology TELKOMNIKA (Telecommunication Computing Electronics and Control) WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Standardisasi

Etih Sudarnika

Divisi Kesehatan Masyarakat Veteriner, Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jl. Agatis Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia 16680

Author-ID : 3033210

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Tingkat Insidensi Malaria di Wilayah Pemanasan Kelambu Berinsektisida Tahan Lama dan Wilayah Kontrol (MALARIA INCIDENCE RATE OF HEAT ASSISTED REGENERATION LONG LASTING INSECTICIDAL NETS AREA AND CONTROL) Etih Sudarnika; Mirnawati Sudarwanto; Asep Saefuddin; Umi Cahyaningsih; Upik Kesumawati Hadi; Rita Kusriastuti; Jodi Vanden Eng; Daowen Zhang; William A. Hawley
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Long lasting insecticidal nets (LLIN) is one effective way to prevent malaria. Permethrin treatedLLIN is one type of LLIN which is recommended by WHO. Several studies have shown that these types ofLLIN requiring heat assisted regeneration after washing to enhance the biological activity of insecticidethat contained in the LLIN fibers. This study aimed to compare the incidence rates of malaria in childrenunder five years old who live in the intervention area (where the heat assisted regeneration on LLIN afterwashing was applied) and control area (where the heat assisted regeneration on LLIN after washing wasnot applied). Data of malaria cases was collected from laboratory log book at all health centers in BangkaDistrict, in the period of June June 2007 until July 2008. Data were analyzed with Poisson regressionmodels. The results showed that the incidence rate of malaria in children under five years old was notsignificantly different between the treatment and control areas.

Resistansi Escherichia coli terhadap Kolistin dan Deteksi Gen Mobilized Colistin Resistance-1 pada Ayam Pedaging Akibat Pemberian Kolistin Sulfat Maria Fatima Palupi; Hera Maheshwari; Huda Salahuddin Darusman; Etih Sudarnika; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Colistin sulphate is the ultimate antimicrobial choice for the treatment of multidrug resistance gram negative bacteria infections with in human. The purposes of this study were to detect the presence of colistin resistant E. coli and mcr-1 gene in broiler and to transfer the mcr-1 gene to Salmonella enteritidis ATCC 13076. A total of 54 one day old broilers were divided into three groups that consists of 18 chicks broiler per group and raised up to 40 days old. The first group was used as control. The first treatment group was given colistin sulphate 5 ìg/g feed for 40 days and broilers in second treatment group was given 80.000 IU/kg body weight for first three days. Swab cloaca samples were taken every 10 days from each broiler. At age 40 days all chickens were slaughtered and meat samples were collected. Samples of cloacal swabs, fresh and cooked meat were examined for the presence of colistin resistant E. coli and mcr-1 gene. Susceptibility to colistin sulfate was conducted by agar dilution method, and detection of mcr-1 gene was conducted using polymerase chain reaction. The results showed that no colistin resistant E. coli was detected in the control group. Colistin resistant E. coli (27.78%) and mcr-1 gene (20.00%) were detected in animals in the first treatment group, respectively. Whilst 11.11% colistin resistant E. coli and 5.56% were carriying mcr-1 gene in the second treatment group. Colistin resistant E. coli were found 5.56% from raw meat samples and 3.70% had mcr-1 gene. Transfer of mcr-1 gene from colistin resistant E. coli to Salmonella enteritidis ATCC 13076 was success. These results showed the necessity of limitation usage of colistin sulphate in food animal.

Pengetahuan, Sikap, dan Praktik Pemilik Anjing Terkait Rabies di Kabupaten Sukabumi, Jawa Barat (KNOWLEDGE, ATTITUDE, AND PRACTICE STUDY OF DOG OWNERS RELATED TO RABIES IN SUKABUMI DISTRIC, WEST JAVA) Ardilasunu Wicaksono; Abdul Zahid Ilyas; Etih Sudarnika; Denny Widaya Lukman; Yusuf Ridwan
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Sukabumi District is one of the districts in West Java Province that has not been rabies-free since there are still reported rabies dog bites and rabies-positive cases in humans. The aim of the study was to measure the knowledge, attitude and practice of dog’s owner towards rabies which will then be useful for designing the appropriate rabies control programs. A total of 141 dog owners were selected as samples/ respondents. Respondents were taken from two sub-districts in Sukabumi District, which were 66 people from Jampang Tengah Sub-district and 75 people from Cisolok Sub-district. The data were taken using structured questionnaire. The result showed that most of respondent’s level of knowledge (51.1%) was in moderate category, meanwhile the respondent’s level of attitude related to rabies control program mostly (69.5%) was in positive category. But the positive attitude was not reflected with their practice towards rabies control program which many of them (63.1%) was in bad category of rabies control practices. From analytical result showed that only one characteristic variablewhich was the information access about rabies that had significant correlation (c2=9.959, p=0.006) towards respondent’s level of knowledge. Furthermore, another result showed that there was a significant relationship between knowledge variable with respondent’s attitude level (p= 0.000, r= 0.275).

SEKUENSING GEN MCR-1 DARI ESCHERICHIA COLI DAN SALMONELLA ENTERITIDIS RESISTAN KOLISTIN Maria Fatima Palupi; Ernes Andhesfa; Hera Maheshwari; Huda Salahuddin Darusman; Etih Sudarnika; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Gen mobilized colistin resistance (mcr) merupakan gen resistan kolistin sulfat yang bisa dipindahkan melalui materi genetik bergerak misalnya plasmid. Tujuan dari penelitian ini adalah mengetahui jenis plasmid gen mcr-1 yang berhasil dipindahkan dari satu isolat Escherichia coli resistan kolistin ke Salmonella Enteritidis ATCC 13076 (dua isolat), dan satu isolat E. coli O157:H7 resistan kolistin positif gen mcr-1. Sekuensing gen mcr-1 kemudian dianalisis di unit Biotek Balai Besar Pengujian Mutu dan Sertifikasi Obat Hewan dengan menggunakan basic local alignment search tools (BLAST) dan software MEGA7. Hasil sekuensing gen mcr-1 dengan panjang nukleotida 309 memiliki homologi tinggi dengan data referen dari Genbank pada gen mcr-1 yang terdapat dalam plasmid. Hasil sekuensing DNA gen mcr-1 sampel lebih cenderung termasuk pada tipe plasmid IncI2 atau IncHI2. Hal ini menunjukkan bahwa resistansi kolistin merupakan ancaman yang nyata karena gen resistan mcr-1 yang ada di Indonesia benar berada di plasmid sehingga mudah disebarkan ke bakteri lain. Kata kunci: mcr-1, plasmid, resistansi ABSTRACT The mobilized colistin resistance (mcr) gene is a colistin sulfate resistance gene that can be transferred through mobile genetic material such as plasmids. The purpose of this study was to determine the type of plasmid mcr-1 gene that was successfully transferred from an isolate of colistin-resistant Escherichia coli to Salmonella Enteritidis ATCC 13076 (two isolates), and one colistin-resistant E. coli O157: H7 that positive mcr-1 gene. The sequencing of the mcr-1 gene was then analyzed in the Biotek unit at National Veterinary Drug Assay Laboratory using basic local alignment search tools (BLAST) and MEGA7 software. The sequencing of the mcr-1 gene with nucleotide length 309 has a high homology with the reference data from Genbank on the mcr-1 gene contained in the plasmid. DNA sequencing of mcr-1 gene samples is more likely to belong to the IncI2 or IncHI2 plasmid types. This shows that colistine resistance is a real threat because the mcr-1 resistance gene in Indonesia is actually in the plasmid so it is easily spread to other bacteria. Keywords: mcr-1, plasmid, resistance

Faktor Risiko Terkait Manajemen Kesehatan Unggas terhadap Infeksi Virus Flu Burung di Tempat Penampungan Ayam (THE RISK FACTOR OF POULTRY HEALTH MANAGEMENT TO THE INFECTION OF AVIAN INFLUENZA VIRUS IN POULTRY COLLECTING FACILITIES) Chaerul Basri; Zudanang .; Sunandar .; Etih Sudarnika
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of the study was to determine the association between the implementation of poultryhealth management and the spread of avian influenza virus in Poultry Collecting Facilities (PCFs). Thestudy was performed by maintaining 7-8 sentinel chickens in 39 PCFs in Jakarta for three months. Thevariables evaluated for poultry health management were health certificate, health inspection, healthinspector, health inspection method, and handling of sick and dead birds. Data on the variables werecollected by interview with supervisor of PCFs. The AIV infection were detected by rt-PCR from the cloacaland tracheal swab of the dead birds with. The results showed that the methodes of handling of sick birdswere significantly associated with infection of AIV (RR=2,00 ; 95% CI = 1,31-3,05). The other variables didnot show significance association. The risk of AIV infection was twice higher the sick keeping side birdsalive, or by separating, or treating the birds in the same cage than by slaughtering them. Poultry healthmanagement in PCFs need to be improved in order to prevent and control the spreading of AIV in Indonesia.

SeroprevalensiTrichinellosispadaBabi di Kota Kupang, Provinsi Nusa Tenggara Timur (SEROPREVALENCE OF TRICHINELLOSIS ON PIG AT KUPANG CITY, PROVINCE OF NUSA TENGGARA TIMUR) Andrijanto Hauferson Angi; Fadjar Satrija; Denny Widaya Lukman; Mirnawati Sudarwanto; Etih Sudarnika
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Trichinellosisis is a zoonotic disease caused by infection of Trichinella spp. and is found world-wide.The seroprevalence of trichinellosis in pig population has never been reported in East Nusa TenggaraProvince particularly Kupang City. The aim of this study was to determine the seroprevalence oftrichinellosis in pig at Kupang City, East Nusa Tenggara. A cross-sectional study was conducted byexamining 376 blood samples of pigs collected from slaughterhouses in Oeba, Kupang using simplerandom sampling method. Blood samples were tested by indirect enzyme linked immunosorbent assay(ELISA) using Screen®Trichinella indirect multi-species ELISA kit. Test results showed that 3 (0.8%)serum samples were positive of trichinella antibody. In conclusion ,some pigs in the city of Kupang hasbeen infected by Trichinella sp. and could be a threat for human health.

Pengetahuan, Sikap, dan Praktik Pemilik Breeding Kennel terhadap Pencegahan dan Pengendalian Bruselosis pada Anjing Impor (KNOWLEDGE, ATTITUDE, AND PRACTISES OF BREEDING KENNEL OWNER REGARDING CANINE BRUCELLOSIS PREVENTION AND CONTROLLING ON IMPORTED DOGS Citra Noviana; I Wayan Teguh Wibawan; Etih Sudarnika
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This study was conducted to determine the seroprevalence of Brucella canis of imported dog for breederthrough Soekarno Hatta International Airport, Cengkareng Tanggerang, Indonesia. A total of 64 serumsamples were obtained from breeder dogs imported to Indonesia between Mei–September 2014. Theserums were examined by Immunochromatographic Assay. None of the serum samples in this studytested positive. Result of this study indicated that antibodies of B. canis were not detected in 64 dogsimported to Indonesia during the research period. A Knowledge, Attitude and Practises (KAP) Survey wasalso performed. This study used 32 respondents and data were analyzed by using path analysis. Therespondents were breeding kennel owners (breeder) who imported dogs through Soekarno HattaInternational Airport during the research period. The study concluded that the knowledge significantlyinfluenced the attitude of the breeder, and also the attitude significantly influenced the practises ofpreventing and controlling brucellosis. The annually income was also identified as a variable thatsignificantly influenced the practises. Practice prevention and control of canine brucellosis can be improvedby increasing the knowledge of the breeding kennel owner.

Pengetahuan, Sikap, dan Praktik Pemilik Anjing Terhadap Kejadian Rabies di Kabupaten 50 Kota, Sumatera Barat Mardi Fadillah; Etih Sudarnika; Mirnawati Bachrum Sudarwanto
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Rabies merupakan penyakit hewan menular strategis prioritas di Indonesia karena berdampak terhadap sosial ekonomi dan kesehatan masyarakat. Berdasarkan laporan kejadian rabies di Kabupaten 50 Kota, Sumatera Barat, beberapa kecamatan menunjukkan angka kejadian yang cukup tinggi setiap tahunnya. Penelitian ini bertujuan untuk mengukur tingkat pengetahuan, sikap, dan praktik pemilik anjing terhadap rabies. Sebanyak 96 responden dipilih sebagai sampel yang diambil dari delapan kecamatan dari 13 kecamatan yang ada di Kabupaten 50 Kota. Metode pengambilan data menggunakan kuesioner yang telah disusun secara terstruktur dan selanjutnya diolah secara deskriptif dengan analisis korelasi menggunakan Uji Chi-Square dan Korelasi Spearman. Hasil penelitian menunjukkan tingkat pengetahuan dan sikap pemilik anjing terhadap rabies berada dalam kategori baik (87,5% dan 94,8%). Hal tersebut berbeda dengan praktik pemilik anjing yang berada dalam kategori buruk (63,5%). Hasil analisis lain menunjukkan bahwa ada peubah karakteristik yang memengaruhi praktik pemilik anjing yaitu jumlah dan jenis anjing yang dipelihara. Semakin banyak anjing yang dipelihara maka semakin baik praktik responden. Responden yang memiliki jenis anjing campuran/ras, membuat praktik responden semakin baik. Pengetahuan akan memengaruhi sikap, namun pengetahuan dan sikap tidak berpengaruh terhadap praktik pemilik anjing. Praktik responden mencerminkan kurangnya kegiatan sosialisasi maupun komunikasi, informasi dan edukasi (KIE), diperlukan penekanan program penyuluhan rabies lebih lanjut terhadap pemilik anjing yang berfokus pada tanggung jawab terhadap kepemilikan anjing. Dengan demikian, untuk meningkatkan tingkat praktik responden tidak cukup dengan pengetahuan dan sikap saja, diperlukan regulasi hukum dan sanksi yang jelas serta prosedur operasional baku mengenai pengendalian rabies dari pemerintah yang sangat penting untuk mengurangi infeksi rabies di Kabupaten 50 Kota.

Pengembangan Media Padat untuk Menumbuhkan Mycobacterium bovis (DEVELOPMENT OF SOLID MEDIUM FOR MYCOBACTERIUM BOVIS CULTIVATION) Mazdani Ulfah Daulay; Mirnawati Sudarwanto; Widagdo Sri Nugroho; Etih Sudarnika
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Mycobacterial culture provides definitive diagnosis of tuberculosis (TB), but commercially readyto-use culture media for Mycobacterium bovis are rarely available. The aims of this study were todevelop and to evaluate the ability of M. Bovis to grow in Modified Ogawa Agar (MOA) in comparisonwith the available culture media, such as Löwenstein Jensen (LJ) and Modified Ogawa (MO). Eachmedia were inculation with 0.1 ml suspension of 105 CFU/mL M. bovis and M. phlei in PhosphateBuffer Saline (PBS) and each media was replicated in five tubes. Mycobacterium phlei grew in everymedium since day 4. M. bovis grew in media LJ and MO since day 17, but failed to grow in mediumMOA. The recovery rate of M. phlei in LJ and MOA were significantly different. The ability of MOA tocultivate M. phlei was different from LJ. Colonies of M. phlei in MOA were easier to be harvested, muchsimpler to prepare, and more feasible than medium LJ. The recovery rate of M. bovis in media LJ andMO were not significantly different, but medium MO were much simpler to prepare and more feasiblethan medium LJ. Media MOA were able to cultivate M. phlei, but proven unable to cultivate M. bovisin this research.

Judul Seroprevalensi Trichinellosis pada Ternak Babi di Wilayah Kabupaten Tangerang, Propinsi Banten (SEROPREVALENCE OF TRICHINELLOSIS IN PIGS IN THE TANGERANG DISTRICT PROVINCE OF BANTEN) Evie Setyani; Fadjar Satrija; Etih Sudarnika
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Trichinellosis is a zoonotic disease caused by parasitic roundworms of the nematode class Trichinella spp, which is distributed worldwide. Indonesia is one of the countries with trichinellosis cases. This study aims to determine the seroprevalence of Trichinellosis in pigs in Tangerang Village, Banten Province. The sample size is determined with 95% confidence level, the expected prevalence is 5%, and the error rate is 5%. A total of 80 sera were collected from farm (27 sera) and from the sample banks of Disease Investigation Centre Subang (DIC Subang(53 sera)). The sample collection was examined serologically by the ELISA method for the examination of anti-Trichinella antibodies. The results showed that seroprevalence of trichinellosis in this study was 1.25% (confidence interval (CI) 95%; 0,22-6,75%). This case can be a threat to human health. Pigs that show seropositive results were the pigs with traditional husbandry practices with ground flooring and without fences. The results of the questionnaires that distributed to the farmers indicated that all farmers did not know about trichinellosis, this may affect the presence of Trichinella in pigs.

Co-Authors . Sumitro Abdul Zahid Adi Winarto Afiff , Usamah Agus Wijaya Amiruddin Saleh Andi Eka Putra Andi Eka Putra Andrijanto Hauferson Angi Anggraini, Raden Isma Annisa Madyanti Geminastiti Parampasi Apriana, Cut Desna Ardilasunu Wicaksono Asah Hilaliah ASEP SAEFUDDIN Bambang Sumiarto Bambang Sumiarto Chaerul Basri Citra Noviana Danang Dwi Cahyadi Daowen Zhang Denny Lukman Denny Widaya Lukman Denny Widya Lukman Dinar Munggaran Akhmad Diyan Cahyaningsari Dwida Agustina Suherman Edi Sukmawinata Ernes Andhesfa Evie Setyani F .X. Koesharto Fitri Hidayati Hadri Latif Hadzash Peppyrhanggasidhi Shatyaayyupranathasari Hasya, Karin katina HERA MAHESHWARI Heris Kustiningsih Heru Setijanto Herwin Pisestyani Hidayah, Dinda Nur Huda Salahuddin Darusman Huda Shalahudin Darusman I wayan Teguh Wibawan Iccha Pradipta Patsiwi Isrok Malikus Sufi Jodi Vanden Eng Katyusa Nandini Dyah Pitaloka koekoeh santoso Lymbran Tina Manuel da Costa Mardi Fadillah Maria Fatima Palupi Maria Fatima Palupi Maria Fatima Palupi Maria Fatima Palupi Mazdani Ulfah Daulay Mirnawati B Sudarwanto Mirnawati B Sudarwanto1 Mirnawati B. Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto Mirnawati Sudarwanto MIRNAWATI SUDARWANTO Mirnawati Sudarwanto Mirnawati Sudarwanto Muhammad Pauzi Lubis Mujiatun Mujiatun Murniati . Murniati Murniati Nararya Adinata Nisa’, Chairun Nugraha, Arifin Budiman Nuraini , Henny Nurhidayat Nyayu Lathifah Tirdasari Okti Nadia Poetri Pramuwidyatama, M. Gumilang Prima Mei Widiyanti prima mei widiyanti, prima mei R. Dikky Indrawan Rachmi Ramadhanita Raden Dikky Indrawan Rakhmat, Asep Raphaella Widiastuti raphaella widiastuti Retno Damajanti Soejoedono Retno Damayanti Soejoedono Risma Juniarti Silitonga Risqika Akla Velayati Rita Kusriastuti Ronald Tarigan Rosmanah, Lis Rotinsulu, Dordia Anindita Roza Azizah Primatika Roza Azizah Primatika S Dwidzuriputra Santi Lestari Satriyo Setyo Utomo Saukhan, Falih Prenata Savitri Novelina Septa Walyani Siti Istiqomah sri murtini . Srihadi Agungpriyono Sudarwanto, Mirnawati B Sudarwanto, Mirnawati Bachrum Sufi, Isrok Malikus Sunandar . Supratikno Susan Maphilindawati Noor Susi Soviana Taufiqqurrahman, Mas Trioso Purnawarman Umi Cahyaningsih Upik Kesumawati Hadi UUS SAEPULOH Wasmen Manalu Widagdo Sri Nugroho widiastuti, raphaella Widiyanti, Prima Mei Widyadhari, Atsmarina William A. Hawley Yusuf Ridwan Zudanang .

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