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All Journal Jurnal Ilmu Pertanian Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Kesehatan Andalas agriTECH Jurnal Teknik Sipil BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) ALCHEMY Jurnal Penelitian Kimia Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Kimia Terapan Indonesia ANNALES BOGORIENSES OLDI (Oseanologi dan Limnologi di Indonesia) Marine Research in Indonesia Jurnal Riset Biologi dan Aplikasinya Pharmaceutical Sciences and Research (PSR) Makara Journal of Science Jurnal Bioteknologi & Biosains Indonesia (JBBI) Annales Bogorienses Jurnal Bioteknologi & Biosains Indonesia
PUSPITA LISDIYANTI
Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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UTILIZATION OF INDONESIAN STRAIN ACTINOBACTERIA TO FACE THE THREAT OF BIOFILM AS THE MAIN CAUSE OF HEALTH CARE ASSOCIATED INFECTION Cahyo, Bian Dwi; Retnowati, Dwi; Lisdiyanti, Puspita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13111

Abstract

Health Care Associated Infection (HCAI) is one of the global infectious diseases that is expected to cause around 10 million deaths by 2050. One of the main causes is biofilm, an exopolysaccharide layer formed by bacteria, often found on medical equipment such as catheters, and has high resistance to antibiotics. Prevention efforts can be made through the search for antibiofilm compounds. Actinobacteria are known to produce potential bioactive compounds. This study utilized Actinobacteria strains from the waters of Bitung, North Sulawesi (code BT-023-026) to explore their antibiofilm potential through isolation, laboratory tests, and genetic analysis, followed by expert confirmation in the fields of intelligence, BPOM, and microbiology. The results show the great potential of BT-023-026 as an antibiofilm agent to prevent antibiotic resistance due to HCAI, while playing a strategic role in biodefense and strengthening health security through early warning and early detection.
UTILIZATION OF INDONESIAN STRAIN ACTINOBACTERIA TO FACE THE THREAT OF BIOFILM AS THE MAIN CAUSE OF HEALTH CARE ASSOCIATED INFECTION Cahyo, Bian Dwi; Retnowati, Dwi; Lisdiyanti, Puspita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13111

Abstract

Health Care Associated Infection (HCAI) is one of the global infectious diseases that is expected to cause around 10 million deaths by 2050. One of the main causes is biofilm, an exopolysaccharide layer formed by bacteria, often found on medical equipment such as catheters, and has high resistance to antibiotics. Prevention efforts can be made through the search for antibiofilm compounds. Actinobacteria are known to produce potential bioactive compounds. This study utilized Actinobacteria strains from the waters of Bitung, North Sulawesi (code BT-023-026) to explore their antibiofilm potential through isolation, laboratory tests, and genetic analysis, followed by expert confirmation in the fields of intelligence, BPOM, and microbiology. The results show the great potential of BT-023-026 as an antibiofilm agent to prevent antibiotic resistance due to HCAI, while playing a strategic role in biodefense and strengthening health security through early warning and early detection.
Selection of Proteolytic Lactic Acid Bacteria and the Potential as α-Glucosidase Inhibitor Activity During Milk Fermentation Miftakhussolikhah, Miftakhussolikhah; Utami, Tyas; Lisdiyanti, Puspita; Rahayu, Endang Sutriwati
agriTECH Vol 46, No 1 (2026)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.100617

Abstract

Diabetes mellitus (DM) is a degenerative condition increasing globally, including in Indonesia, with a corresponding rise in death rates. One of the therapy methods for DM is the use of α-glucosidase inhibitor (AGI), with peptides identified as potential AGI-based food ingredients. Therefore, this study aimed to develop fermented milk products enhanced with AGI peptides by screening and identifying lactic acid bacteria (LAB) strains with proteolytic capabilities. The experiment was conducted in three main stages: (1) screening 32 LAB isolates for their proteolytic activity, (2) determining the optimal fermentation time for AGI production using selected proteolytic LAB strains, and (3) molecular identification of the selected LAB strains through 16S rRNA gene sequencing. The results showed that two isolates, SR17B and L23, identified as Lactiplantibacillus plantarum-pentosus SR17B and Lactiplantibacillus plantarum-pentosus L23, had high proteolytic activity and were capable of producing fermented milk with significant AGI activity of 35.94% and 35.15%, respectively. AGI activity progressively intensified during fermentation, peaking at 12 hours in both strains, indicating that this period of time was ideal for fermentation in order to have the largest inhibitory effect. These results suggested that selected LAB strains, particularly L. plantarum-pentosus SR17B and L23, could serve as functional starter cultures for development of fermented dairy products with antidiabetic potential.
Fermentation Effect of Cacao Beans Originate from Jember on Polyphenol-Flavonoid Content and Radical Scavenging Activity Agustriana, Eva; Valentino, Herly Angga; Rahmani, Nanik; Nuryati, Nuryati; Firmanto, Hendy; Rachmayati, Rike; Yulianti, Siti Eka; Nuryana, Isa; Yopi, Yopi; Lisdiyanti, Puspita
ALCHEMY Jurnal Penelitian Kimia Vol 19, No 1 (2023): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.19.1.60831.23-31

Abstract

Cacao is attractive for its flavor and taste and its beneficial effects. Therefore, this commodity is promising to be developed as a functional food. Many studies reported the bioactive compounds in cacao beans and their health benefits. However, to attain desirable flavor and taste, the curing process of cacao beans is a common practice, in which fermentation is one of the processes. Despite its significance, fermentation also alters the bioactive components in cacao beans. To develop a functional food rich in bioactive compounds, measuring the bioactive contents of treated and untreated cacao beans is desired. For that reason, in this study, the analysis of total polyphenol, flavonoid, catechin, and epicatechin, as well as the radical scavenging activity of fermented cacao beans, was performed. The study revealed that fermentation negatively affected all parameters. Cacao beans fermentation up to 96 h resulted in the loss of 54% of total polyphenol, 77% of total flavonoid, and 56% of radical scavenging activity. Determination of the flavan-3-ol components, catechin, and epicatechin,  revealed that they were reduced as the fermentation occurred.
Co-Authors - Yopi Abd. Rasyid Syamsuri ADE ANDRIANI Adi Santoso Agustinus Robert Uria Ahmad Thontowi Akhmad Rivai Andi Utama ANDI UTAMA ANDO KATSUHIKO Apriliana, Pamella Ariani Hatmanti Ario Betha Juanssilfero Ashadi Sasongko Atikana, Akhirta Cahyo, Bian Dwi Caniago, Asnany Caniago, Asnany Desriani Desriani Desriani Desriani Dewi Seswita Zilda dinihari indah kusumawati Dwi Retnowati Dwi Wulandari Elvi Yetti Endang Saepudin, Endang Endang Sukara Erdiansyah Rezamela, Erdiansyah Eva Agustriana Evi Triana Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Firmanto, Hendy GINA KARTINA Hastuti, Ratih Dwi Herman Suryadi JAKA WIDADA KATSUHIKO ANDO Krause, Janina Kusharyoto, Wien Kusharyoto, Wien Kusumawardhani, Dinihari Indah Kusumawati, Dinihari Indah Kusumawati, Dinihari Indah Lina Elfita Lina Elfita LINDA SUKMARINI M. Iqbal Prawira-Atmaja, M. Iqbal Mangunwidjaja, Wibowo MARIA BINTANG Maulidia, Faiqoh Nur Meliah, Siti Meliah, Siti Mia Kusmiati Miftakhussolikhah, Miftakhussolikhah Miranti Nurindah Sari, Miranti Nurindah MISA OTOGURO Mochammad Imron Awalludin Mulyorini Rahayuningsih Nadhifah, Hana NANIK RAHMANI Nanik Rahmani Neneng Hasanah Neng Herawati, Neng Ni Luh Putu Vidya Paramita NITA ROSALINDA PRAYITNO Nurul Fitri Sari Nuryana, Isa Nuryati Nuryati Prasetyoputri, Anggia Pratama, Rendy Eka Prayoga, Muhammad Bagas Putra, Filemon Jalu Nusantara Putra, Masteria Yunovilsa Putri, Ade Lia Putri, Ade Lia Rachmayati, Rike Rahayu, Endang Sutriwati Restu Wulansari, Restu Riyona Desvy Pratiwi, Riyona Desvy ROHMATUSSOLIHAT ROHMATUSSOLIHAT RONI RIDWAN Seprianto SHANTI RATNAKOMALA Shanti Ratnakomala Shanti Ratnakomala Subagus Wahyuono Suharsono Suharsono Sukma Nuswantara Susanti, Agustina Eko Syafriana, Vilya Tisnadjaja, Djadjat Tisnadjaja, Djadjat Titik Nuryastuti TOMOHIKO TAMURA Toto Iswanto TRI RATNA SULISTIYANI TRI RATNA SULISTIYANI Triana Hertiani Trisanti Anindyawati, Trisanti Tutik Murniasih Tyas Utami Ukhradia Maharaniq Safira Untari, Febriana Valentino, Herly Angga WIBOWO MANGUNWARDOYO Yana Rubiyana, Yana Yantyati Widyastuti Yopi Y YOPI YOPI Yopi Yopi Yopi, Yulianti, Siti Eka YULIN LESTARI Yusro Nuri Fawzya