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PENGARUH DIKLOFENAK PADA HEPAR TIKUS (Rattus norvegicus) TERHADAP LAJU PERTUMBUHAN LARVA Chrysomya megacephala dalam PENENTUAN POST MORTEM INTERVAL Farah Aini Adiba; Ida Kinasih; Ucu Julita
Gunung Djati Conference Series Vol. 47 (2024): Seminar Nasional Biologi (SEMABIO) ke-9 Tahun 2024
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pengaruh toksik yang dialami Calliphoridae menjadi isu utama dalam entomologi forensik sebagai bukti kualitatif untuk menentukan obat atau toksik yang dapat memberikan petunjuk dalam penyelidikan forensik, seperti estimasi Post Mortem Interval (PMI). Tujuan dari penelitian ini adalah mengetahui pengaruh diklofenak terhadap laju pertumbuhan larva Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae). Media perkembangan Chrysomya megacephala menggunakan hepar tikus strain Wistar dengan proporsi 9 gram yang telah dicampur dengan campuran diklofenak. Diklofenak digunakan dalam tiga konsentrasi: 25 mg/9 gram untuk dosis 1, 50 mg/9 gram untuk dosis 2, dan 75 mg/9 gram untuk dosis 3. Untuk membandingkan hasil, sampel kontrol menggunakan hepar (9 gram) tanpa campuran diklofenak. Tiap perlakuan dilakukan sebanyak 3 kali pengulangan. Bobot dan panjang larva Chrysomya megacephala diukur sebanyak 5 kali setiap 3 hari sekali selama percobaan. Pengamatan durasi siklus Chrysomya megacephala diamati tiap hari. Penelitian menemukan bahwa larva pada tiap perlakuan dosis lebih pendek ukurannya dibandingkan dengan kontrol. Semua perlakuan dosis mengalami penurunan bobot larva Chrysomya megacephala secara signifikan. Semua perlakuan dosis diklofenak menyatakan perpanjangan masa perkembangan Chrysomya megacephala. Sampel kontrol menyelesaikan perkembangannya dalam waktu 267,7 jam, lebih singkat dibandingkan dengan perlakuan dosis terendah hingga tertinggi: 384,7 jam (P1), 408 jam (P2), dan 456 jam (P3). Mortalitas larva yang terjadi signifikan pada semua perlakuan dosis. Temuan ini menunjukkan diklofenak menghambat perkembangan Chrysomya megacephala hingga 117 jam lamanya, semakin tinggi dosis diklofenak maka semakin memperpanjang siklus perkembangan dan menghambat pertumbuhan larva sehingga dapat berdampak pada estimasi Post Mortem Interval (PMI).
The Digestibility of Banana Peel and Testa coconut and Its Effects on the Growth and Mortality of Black Soldier Fly Larvae (Hermetia illucens) at Constant Feeding Rates Putra, Ramadhani Eka; Margareta, Alfariana; Kinasih, Ida
Biosfer: Jurnal Tadris Biologi Vol 11 No 1 (2020): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v11i1.6450

Abstract

Organic waste produced by economic activities may create health, aesthetic, and economic problems. One of the approaches applied to solve this problem is the utilization of decomposer macrofauna to decompose the waste. One of the decomposers with great potential is Black Soldier Fly larvae (Hermetia illucens) that can consume various types of organic wastes and converts it into biomass with high protein and lipid content. In this study, banana peels and coconut testa had been fed to the larvae at 200 mg/larvae/day as the objects that represented organic wastes with low fiber content and high fiber content respectively. The purpose of this study was to observe the growth and efficiency of BSF larvae in decomposing those wastes. The analysis was conducted on some parameters such as the growth and consumption rate, the efficiency of conversion of digested (ECD), waste reduction index, and mortality rate. The results showed that BSF larvae THAT consumed banana peel had a higher final weight (58.24 mg), growth rate, and consumption rate while the mortality rate was lower than BSF larvae that consumed coconut testa. The ECD of the larvae group that consumed banana peel was higher than the larvae group that consumed coconut testa. The waste reduction index of banana peel was higher than coconut testa (1.5 and 1.4, respectively). The larvae that consumed coconut testa had a longer pupation period (9±1,75 days) compared to the larvae that consumed banana peel. Based on this result, it can be concluded that the fiber content of organic waste affected the decomposition rate and growth of BSF larvae.
Efisiensi Biokonversi dan Produktivitas Black Soldier Fly pada Medium Sampah Organik Sisa Makanan: Kontribusi terhadap Pengembangan Pakan Ternak Lokal Julita, Ucu; Kinasih, Ida; Rasyid, Sayyidah
Jurnal Teknologi Hasil Peternakan Vol 6, No 2 (2025): September
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jthp.v6i2.61004

Abstract

Pengelolaan sampah organik, terutama sisa makanan, merupakan tantangan besar di Indonesia. Larva lalat tentara hitam (Black Soldier Fly/BSF) menawarkan solusi dengan kemampuannya mereduksi limbah organik dan menghasilkan biomassa bernutrisi tinggi yang berguna sebagai pakan ternak alternatif. Penelitian ini bertujuan untuk mengevaluasi pengaruh pemberian sampah organik sisa makanan terhadap efisiensi biokonversi dan reproduksi BSF. Tiga perlakuan diuji, yaitu kontrol (pakan ayam), sampah organik non-fermentasi, dan sampah organik fermentasi, dengan tiga pengulangan. Pengamatan meliputi pertumbuhan, proporsi konversi pakan, indeks pengurangan limbah (Waste Reduction Index/WRI), efisiensi konversi pakan yang dicerna (Efficiency Conversion of Digestive Feed/ECD) dan tingkat fekunditas. Hasil penelitian menunjukkan biomassa tertinggi pada sampah fermentasi (F100) sebesar 24,225 mg dan nilai ECD tertinggi pada kontrol (38,59%). WRI tertinggi (4,65%) terdapat pada sampah organik fermentasi, fekunditas dan fertilitas tertinggi masing-masing ditemukan pada perlakuan K150 dan F100. Sampah organik fermentasi secara signifikan meningkatkan efisiensi biokonversi, pertumbuhan, dan reproduksi BSF dibandingkan sampah organik non-fermentasi. Temuan ini mendukung penggunaan larva BSF untuk pengelolaan limbah organik yang lebih efisien sekaligus mendukung produksi pakan ternak lokal yang ramah lingkungan. Potensi ini memberikan peluang besar untuk pengembangan peternakan berkelanjutan di berbagai daerah. 
Efisiensi Penyerbukan Tetragonula laeviceps Pada Budi Daya Tumpang Sari Tomat Dan Mentimun Di Greenhouse Putra, Ramadhani Eka; Novitasari, Novitasari; Rosmiati, Mia; Husna, Inayati Zakiyatun; Setiyarni, Eka; Kinasih, Ida
Al-Kauniyah: Jurnal Biologi Vol. 17 No. 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.29769

Abstract

AbstrakPenyerbukan oleh serangga liar merupakan salah satu servis ekosistem yang tidak dapat diperoleh oleh sistem pertanian tertutup. Hal tersebut mungkin dapat digantikan oleh lebah yang didomestikasi, seperti lebah tidak bersengat (Tetragonula laeviceps). Pada penelitian ini, koloni T. laeviceps diuji coba sebagai sistem tumpang sari tomat (Lycopersicon esculentum L.) dan mentimun (Cucumis sativus L.)  yang ditanam pada greenhouse. Kesuksesan penyerbukan diamati pada 100 tangkai bunga tomat dan mentimun oleh koloni T. laeviceps yang ditempatkan pada greenhouse, kemudian dibandingkan dengan kesuksesan penyerbukan pada sistem budi daya yang terdapat di luar greenhouse (penyerbukan terbuka). Untuk dapat menjelaskan peran dari T. laeviceps sebagai penyerbuk maka dilakukan juga pengamatan pada tingkat kunjungan lebah, laju kunjungan, dan waktu yang dihabiskan pada bunga. Efek lanjutan dari proses penyerbukan seperti diameter, panjang, bobot, dan jumlah biji juga diamati dan dibandingkan antara kelompok yang dibudidayakan di dalam dan di luar greenhouse. Efisiensi penyerbukan dan kualitas buah tomat yang dihasilkan di luar greenhouse secara signifikan lebih baik dibandingkan dengan aplikasi lebah tidak bersengat di dalam greenhouse. Efisiensi penyerbukan mentimun dengan T. laeviceps (14%) sangat rendah dibandingkan dengan penyerbukan terbuka (73%), namun kualitas buah yang dihasilkan lebih tinggi walaupun tidak terdapat perbedaan yang signifikan. Di sisi lain, efisiensi penyerbukan tomat dengan T. laeviceps (45%) sangat rendah dibandingkan dengan penyerbukan terbuka (80%), dengan kualitas buah jauh lebih rendah. Berdasarkan hasil dapat disimpulkan bahwa T. laeviceps dapat diaplikasikan sebagai agen penyerbuk pada sistem greenhouse, namun belum dapat menggantikan keuntungan dari variasi dari agen penyerbuk (biotik dan abiotik) yang terdapat pada sistem budi daya di sistem terbuka. Hasil penelitian ini dapat dijadikan dasar untuk mendesain sistem produksi buah di dalam greenhouse yang berkesinambungan melalui aplikasi servis penyerbukan.AbstractClosed system farming system is lack of natural pollination service which might solve the by application of domesticated bees, such as stingless bees (Tetragonula laeviceps) as pollination agent. This hypothesis was tested in this study in which T. laeviceps was applied as pollination agent of tomato (Lycopersicon esculentum L.) and cucumber (Cucumis sativus L.) cultivated as companion plants inside the greenhouse. During this study, pollination efficiency and its effect on fruit quality (such as dimension, weight, and seed numbers) of application of T. laeviceps as a pollination agent were compared to natural pollination systems outside the greenhouse (open pollination). The variables were observed on 100 flowers of both tomato and cucumber cultivated inside and outside greenhouse. The efficiency of pollination by T. laeviceps (14%) on cucumber was significantly lower than open pollination (73%) and the quality of the fruit produced was lower although insignificant. On the other hand, the efficiency of pollination by T. laeviceps (45%) on tomato was also significantly lower than open pollination (80%) and the quality of the fruit produced was significantly lower. Based on this study, although stingless bees have great potency to be applied as pollinators for cultivated crops inside a greenhouse, they still do not completely replace the benefit of pollinator diversity (biotic and abiotic). Furtherly, this study could be applied as a foundation to design a sustainable fruit production inside greenhouse by applying the pollination services. 
Keanekaragaman beserta karakteristik habitat Famili Panorpidae (Ordo: Mecoptera) di Taman Hutan Raya Ir. H. Djuanda Bandung: Diversity and habitat characteristic of Panorpidae family (Order: Mecoptera) in Taman Hutan Raya Ir. H. Djuanda Bandung Ardyanty, Putry; Widiana, Ana; Kinasih, Ida
Jurnal Entomologi Indonesia Vol 20 No 3 (2023): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.19.3.213

Abstract

Mecoptera is a primitive and minor order of insects with nine families, including Panorpidae. Research on Panorpidae in Indonesia is lacking, especially regarding its habitat. Thus, the purpose of this study was to observe the diversity and habitat characteristics of Panorpidae. The study was conducted at Taman Hutan Raya Ir. H. Djuanda Bandung from March to April 2023. The survey method was conducted at plots in three area blocks: the utilization block, the protection block, and the collection block. Panorpidae were collected by hand and sweeping net and then identified based on morphological characteristics. Temperature, humidity, light intensity, and vegetation where Mecoptera are found are identified and recorded. This study collected one genus (Neopanorpa) and five species found in the protection block (there were five species) and the utilization block (there were three species) while none in the collection block. The Penorpidae population was higher at the Protection block than at the Utilization block. Generally, Panorpidae was found in broad-leaf vegetation such as Clidemia sp. and Datura sp., with a canopy that is not too dense. The level of diversity and evenness of both areas was medium. Humidity positively affects the diversity and abundance of Panorpidae, while temperature and light intensity have negative effects. This research shows that Panorpidae likes damp places protected by bush vegetation and under not too dense canopy.
GROWTH OF BLACK SOLDIER FLY LARVAE ( Hermetia illucens ) FED WITH PAK CHOI ( Brassica chinensis ) AND CARP ( Cyprinus carpio ) RESIDUES Permana, Agus Dana; Putra, Ramadhani Eka; Nurulfah, Auliya; Rosmiati, Mia; Kinasih, Ida
BIOTROPIA Vol. 28 No. 2 (2021): BIOTROPIA Vol.28 No.2, Agustus 2021
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2021.28.2.1078

Abstract

One main drawback of the local animal industry is the inavailability of affordable and sustainable protein supply for the livestock. Insect larvae, such as the Black Soldier Fly (Hermetia illucens) larvae (BSFL), have been considered as a protein source which can be produced at a large scale using low cost organic wastes as feeding material. This study was designed to determine the response of BSFL to various waste combinations of vegetable and animal remains, Pak Choi (Brassica chinensis) residues (S) and carp (Cyprinus caprio) fish offal (I)). A total of 540 BSFL were fed with 100 mg/larvae/day combination of vegetable wastes: animal waste 70%: 30% (S > I), 50%: 50% (S = I), and 30%: 70% (S < I). Among the feed combinations, the S < I group showed the best results as it produced the significantly highest weight of BSFL at 122.8 mg/larvae and approximate digestibility of 62.01%, with the least pupae mortality rate at 4.29%.
TRUE SHALLOT (Allium cepa var ascalonicum) SEED PRODUCTION DURING OFF SEASON Putra, Ramadhani Eka; Ramadan, D Beta; Adin, Adriyanita; Kinasih, Ida; Rosmiati, Mia; Oktaviani, Indah; Leksikowati, Sovia Santi
BIOTROPIA Vol. 28 No. 2 (2021): BIOTROPIA Vol.28 No.2, Agustus 2021
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.39 KB) | DOI: 10.11598/btb.2021.28.2.1079

Abstract

The application of seed for true shallot cultivation is an alternative of the more common cultivation practice, in which 30% of harvested tubers used for cultivation purposes. The seed production of this temperate tuber, in the tropical region, is quite challenging due to low flowers and seed formation. Several studies showed that vernalization (cold induction) and application of Benzil Amino Purin (BAP) could be applied to improve flowering and seed production. However, such studies were conducted during the best cultivation period for about 3 months and thus, limit the production period of seeds. This study was conducted to observe the effect of both methods outside cultivation periods to flower and capsule numbers, fruit set, and weight of 100 seeds compared with common cultivation. In this study, bulbs of onion vernalized at 10oC for 30 days then became subjected to synthetic hormone (BAP) prior planted while control group The results showed that BAP treated shallot group has the lowest values for all observed parameters (1552.67, 312.11, 22.5%, 0.2244 gram) compared to those vernalization treated group (1592.44, 623, 30.5%; 0.2261 gram) and control group (6774.67; 3898.44; 57.06%; 0.3304 gram). Based on this study, it could be concluded that common cultivation is a better method to produce true shallot seeds during the offseason.
OMEGA-3 CONTENT OF BLACK SOLDIER FLY PREPUPA (Hermetia illucens) FED WITH MARINE FISH OFFAL AND TOFU DREG Putra, Ramadhani Eka; Fatmalasari, Yenyen; Permana, Agus Dana; Kinasih, Ida; Rosmiati, Mia
BIOTROPIA Vol. 28 No. 1 (2021): BIOTROPIA Vol. 28 No. 1 April 2021
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2021.28.1.1082

Abstract

One of the materials with great potency for future nutrition source for animal feed is prepupae of black soldier fly larvae (BSFP) (Hermetia illucens) which is fed on organic wastes. This study was designed to observe the accumulation of specific substance (omega-3) of organic wastes (marine fish offal and tofu dregs) inside harvested biomass of BSFP. A total of 150 gram food consist of marine fish offal and tofu dreg with different proportion (10:90, 25:75, 50:50, and 100% tofu dregs) was fed to black soldier fly larvae (BSFL) for 21 days.At the end of feeding trial, all BSFL were harvested, weighed, and then analyzed for omega-3 fatty acids content. Fatty acid content was analyzed using Gas Cromatography with Flame Ionization Detector (GC-FID). Result of the experiment showed BSFL reared with 25% marine fish offal produced highest biomass (8.1 g / 50 larvae) with least development time (19 days). The total of omega-3 recorded from application of 0%, 10%, 25%, and 50% of marine fish offal was 0.02%, 0.87%, 2.16% and 2.61% in 100 g of dry weight, respectively. This result showed the possibility of transferring specific nutrient from organic wastes biomass of BSFP which provides base knowledgement for further application in design of specific animal feed from BSFP.
Co-Authors Abdul Rosyad Achadiyani Adawiyah, Ayuni Adin, Adriyanita Adisty Virakawugi Darniwa Afriansyah Fadillah Agus Dana Permana Agus Dana Permana Ahmad Sopian, Ahmad Alfariana Margareta Ana Widiana Ana Widiana Anggita Rahmi Hafsari, Anggita Rahmi ANJARNISA RUSTAM, FAJRINA Ardyanty, Putry Asep Komarudin Ateng Supriyatna Ateng Supriyatna Ateng Supriyatna Ateng Supriyatna, Ateng Azkia, Syifa Raisatul Damanik, Naomi Florenata Dewangga, Rezha Tanu Farah Aini Adiba Fatmalasari, Yenyen Finsa Firlana Gusmara Fitria Nur Aisyah Gunawan, Fikri Iqlilah Hani Hidayah Hermawan, Endang Hery Purnobasuki Hilda Ayu Emalia Hufaizah, Pajar Husna, Inayati Zakiyatun Intan Josefin Purba Julio Subagio Kamilah, Mutiara Mila Kurniawan, Isma Dwi Latama, Zahra Nabila Leksikowati, Sovia Santi Lia Faridah Listia Wati Sophia Margareta, Alfariana Mohamad Agus Salim Mohamad Agus Salim Muhammad Efendi, Muhammad Muhammad Yusuf Nurhadi Nida Hidayaturrohmah Novitasari Novitasari Novitasari Nurmina Arofah Nurul Aulia Fitri Nurulfah, Auliya Oktaviani, Indah Putra, Ramadhani R.C. Hidayat Soesilohadi Raeka Okata Soebakti Rahmat Taufik MA Rahmawati, Tiara Ramadan, D Beta Ramadhani Eka Putra Ramadini Aini Anitasari Rasyid, Sayyidah RIKA RAFFIUDIN Risda Arba Ulfa Risda Arba Ulfa Rizal Maulana Hasby, Rizal Maulana Roma Nugraha Rusputa Rosmiati, Mia Rusdy Syachrul Nugraha Samsul Sulaeman Sari, Anggraeni Arum Sari, Dian Anggria Savira Ekawardhani Setiyarni, Eka SETYARNI, EKA Tri Cahyanto Tri Cahyanto Ucu Julita, Ucu Yani Suryani Yani Suryani Yeyet Setiawati Yuni Kulsum ZAKIYATUN USNA, INAYATI Zhia Rizki Ardian