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Studi Akumulasi Pigmen β-Cryptoxanthin untuk Membentuk Warna Jingga Buah Jeruk di Daerah Tropika Sumiasih, Inanpi Hidayati; Arzam, Taruna Shafa; Poerwanto, Roedhy; Efendi, Darda; Agusta, Andria; Yuliani, Sri
Jurnal Hortikultura Indonesia (JHI) Vol. 9 No. 2 (2018): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (934.285 KB) | DOI: 10.29244/jhi.9.2.73-83

Abstract

ABSTRACTDegreening is a transformation process on peel which enables it to change color from green to orange on citrus fruits. The orange color of the peel comes from the mixture of carotenoid pigments, such as β-cryptoxanthin and β-citraurin. The pigments contributed in the formation of β-citraurin are β-cryptoxanthin and zeaxanthin. The objectives of this study were (1) to obtain proper degreening temperature in the orange color formation of several citrus varieties, and (2) to identify and determine pigments of β-cryptoxanthin pigment and total chlorophyll content in citrus peel after degreening. This study was conducted at PKHT IPB and LIPI Cibinong from July 2013 to December 2013, and from February 2016 to May 2017. About 100 ppm of ethylene gas was injected into a citrus-containing box using 5 ml syringe, then the box was placed in cool storage at 15 0C, 20 0C and room temperature, for 72 hours. The results showed that the best colors of Keprok Selayar and Keprok Tejakula were obtained by the degreening at 15 0C, in Siam Kintamani it was obtained by degreening at 20 0C. Degreening significantly reduced the total chlorophyll content, and increased β-cryptoxanthin content. The content of β-cryptoxanthin after degreening was 3 folds higher on highland Citrus reticulata than lowland citrus.Keywords: citrus color index, chlorophill, degreening, ethylene, tropical citrusABSTRAKDegreening adalah proses perombakan warna hijau pada kulit jeruk diikuti dengan proses pembentukan warna jingga. Warna jingga adalah campuran antara β-cryptoxanthin dengan β-citraurin. Pigmen yang berkontribusi dalam pembentukan β-citraurin adalah β-cryptoxanthin dan zeaxanthin. Tujuan penelitian ini ialah (1) Mendapatkan suhu degreening yang tepat dalam pembentukan warna jingga pada beberapa varietas jeruk, (2) Identifikasi dan penentuan kadar pigmen β-cryptoxanthin dan kandungan total klorofil pada kulit jeruk setelah degreening. Penelitian ini dilakukan di PKHT IPB dan LIPI Cibinong pada bulan Juli 2013 sampai Desember 2013, dan bulan Februari 2016 sampai Mei 2017. Degreening dilakukan dengan menginjeksikan gas etilen konsentrasi 100 ppm ke dalam wadah tertutup yang berisi jeruk menggunakan syringe 5 ml, kemudian disimpan pada suhu 15 0C, 20 0C dan suhu ruang, selama 72 jam. Hasil penelitian menunjukkan bahwa warna terbaik jeruk Keprok Selayar dan Tejakula diperoleh dengan degreening pada suhu 15 0C, Siam Kintamani diperoleh dengan degreening pada suhu 20 0C. Degreening dapat menurunkan kandungan total klorofil secara tajam, dan terbukti meningkatkan kandungan pigmen β-cryptoxanthin. Kandungan pigmen β-cryptoxanthin setelah degreening 3 kali lebih tinggi pada jeruk keprok dataran tinggi dibandingkan dengan dataran rendah.Kata kunci: citrus color index, degreening, etilen, jeruk tropika, klorofil
Appropriate Duration of Drought Stress for Madura Tangerine Flower Induction: Appropriate Duration of Drought Stress for Madura Tangerine Flower Induction Rahayu, Resa Sri; Poerwanto, Roedhy; Efendi, Darda; Widodo, Winarso Drajad
Jurnal Hortikultura Indonesia (JHI) Vol. 11 No. 2 (2020): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.11.2.82-90

Abstract

Cekaman kekeringan merupakan salah satu teknik yang dilakukan untuk menginduksi bunga jeruk keprok di luar musim dalam upaya memenuhi ketersediaan buah jeruk keprok sepanjang tahun. Durasi cekaman kekeringan yang tepat penting dipelajari untuk menghindari cekaman berat dan mendapatkan hasil bunga yang optimum. Penelitian ini bertujuan menentukan durasi cekaman kekeringan yang tepat untuk menginduksi bunga jeruk keprok dataran rendah varietas Madura. Penelitian dilaksanakan di Kebun Percobaan PKHT-IPB dengan ketinggian ± 300 mdpl dari bulan April-Mei 2019. Percobaan dirancang dengan RAK (Rancangan Acak Kelompok) dengan satu faktor perlakuan yaitu durasi cekaman kekeringan dengan lima taraf: tanpa cekaman kekeringan sebagai kontrol, durasi cekaman kekeringan satu minggu, durasi cekaman kekeringan dua minggu, durasi cekaman kekeringan tiga minggu dan durasi cekaman kekeringan empat minggu. Hasil penelitian menunjukkan bahwa durasi cekaman kekeringan mempengaruhi keberhasilan induksi bunga jeruk keprok Madura. Durasi cekaman kekeringan selama 2, 3 dan 4 minggu dengan nilai kadar air secara berturut-turut 81.81%, 65.21% dan 55.39% dari kapasitas lapang berhasil menginduksi bunga. Bunga muncul pada 3, 2 dan 1 minggu setelah rewatering dan pengairan rutin secara berturut-turut pada perlakuan durasi cekaman 2, 3 dan 4 minggu. Tiga minggu tanpa irigasi merupakan durasi cekaman kekeringan optimum untuk induksi bunga jeruk keprok Madura.
Respons fisiologi dan profile metabolit sekunder Lima Kultivar Rambutan (Nephelium lappaceum L.) terhadap Penambahan Cahaya Buatan LED (Light Emitting Diode): Physiology and Secondary Metabolite Profiles Responses of Five Rambutan Varieties (Nephelium lappaceum L.) through Addition of Artificial Light LED (Light Emitting Diode) Irsyad Maulana; Matra, Deden Derajat; Roedhy Poerwanto
Jurnal Hortikultura Indonesia (JHI) Vol. 13 No. 3 (2022): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.13.3.180-186

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Rambutan (Nephelium lappaceum L.) is a fruit that is suitable for growing in the tropics area. However, information on physiological responses and secondary metabolite profiles on artificial light treatment has not been reported. This study aims to study the response of rambutan plants to the application of artificial light from LED (Light Emitting Diode). The study was conducted in the screenhouse nursery using a two-factor nested plot design, namely LED as light source and cultivar. The LED light sources used red, blue, and white with application duration for 21 hours while the cultivars used Sikoneng, Parakan, Cilebak, Gravel, and Garuda cultivars. The results showed that rambutan plants that were given additional blue LEDs contained higher chlorophyll and leaf as glucose and fructose compared to another treatments. The results of the secondary metabolite profiles showed the largest Neophytadiene compound found in the Parakan cultivar under LED red treatment. Meanwhile, the compound that has the greatest abundance in white LEDs is 1-Propene, 3-[(4-nitrobutyl)thio]. Keywords: chlorophyll, photosynthesis, glucose, light spectrum, secondary metabolite
Sensory Evaluation of the Quality of Kaffir Lime (Citrus hystrix DC.) Leaves Exposed to Different Postharvest Treatments Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efedi, Darda; Agusta, Andria
Journal of Tropical Crop Science Vol. 8 No. 02 (2021): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.8.02.71-79

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This study aimed to evaluate the sensory attributes such as aroma, color and texture of kaffir lime leaves in response to various post-harvest treatment. The hedonic test approach was conducted by inviting 70 untrained panelists on seven post-harvest treatments, i.e. (D1) post-sortation fresh leaves, (D2) pre-sortation fresh leaves, (D3) cold-storage leaves, (D4) low temperature-storage leaves, (D5) brown dry leaves, (D6) fresh leaf-flour, and (D7) brown dry leaf-flour. The result showed that most of panelist agreed that aroma was the most important quality attribute that determined the level of preference in kaffir lime leaf products. Among seven tested products, the aroma, color, texture and overall impression of D1 was the most favorite one, while D4, D5, and D7 were assessed as low preference products. This work showed the importance of sortation and cold storage to maintain consumer likeliness. The criteria for sortation were green, clean, pest-disease free, scar free, high uniformity and fresh condition of leaves. Cold-storage at -20oC maintained the color and texture of kaffir lime leaves better than low temperature storage (5oC). To make leaf flour, the use of fresh green leaves was significantly better than brown dry ones. This finding might become the baseline data for the development of kaffir lime leaf product in the future.
The Effects of Preharvest Mild Shading on the Quality and Production of Essential Oil from Kaffir Lime Leaves (Citrus hystrix) Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efendi, Darda; Agusta, Andria
Journal of Tropical Crop Science Vol. 9 No. 01 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.01.15-21

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Kaffir lime (Citrus hystrix DC) is a less popular citrus species commonly used as a food spice and a source of essential oil. Early studies report the success of preharvest mild shading to increase leaf yield, although there is still limited information on the effect of preharvest shading on the quality of essential oil produced. The aim of this current study is to evaluate the effect of preharvest mild shading factors on the yield, physical characteristics, and metabolite fingerprinting of kaffir lime leaves essential oil (KLLEO). One-year-old kaffir lime trees were sampled in two preharvest treatments, i.e., open sun and mild shading (24% light reduction) at Pasir Kuda experimental field, Bogor, Indonesia. Statistical analysis showed that there was no significant effect of preharvest treatment on yield and physical characteristics (color, specific gravity, and refractive index) of KLLEO. In contrast, there was a metabolite fingerprinting variation of KLLEO as an effect of mild shading. The relative percentage of bergamol, citronellol, caryophyllene oxide, citronellic acid, isopulegol, isopulegyl formate, limonene, linalool, and linalool oxide was increased by mild shading. On the other hand, the main metabolite (citronellal) was significantly reduced by about 10% in shading treatment, as compared to the open-sun ones.
Yield and Physicochemical Characteristics of Kaffir Lime Leaf Essential Oils Subjected to Different Post-Harvest Treatment Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efendi, Darda; Agusta, Andria; Rofiq, Muhamad Abdul
Journal of Tropical Crop Science Vol. 11 No. 02 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.02.97-104

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The importance of kaffir lime leaf as essential oils (EOs) raw material is starting to get attention because of its commercial value; however, there is no quality reference for kaffir lime leaf EOs, especially in response to various post-harvest handlings. This study aimed to describe the physicochemical characteristics and yield of kaffir lime EOs subjected to different post-harvest. Bogor originated-kaffir lime leaf was prepared to be subjected to several post-harvest treatments, i.e., control/fresh green leaves (P1); milling to produce green leaf flour (P2); drying to produce dry brown leaf (P3), and milling and drying to produce brown leaf flour (P4). The result showed that post-harvest treatment generally decreases an oil yield and increases darkness color, specific gravity and refractive index of tested EOs. Additionally, post-harvest treatment also changes metabolite profile revealed by GCMS analysis. The relative percentage of caryophyllene and citronellol tends to increase, while the linalool and citronellal levels decrease due to tested post-harvest treatment. It was implied that for the benefit of the fragrance industry with a high citronellal requirement, EOs should be made from fresh green leaves and the leaves should be avoided from drying and powdering treatment.
Pertumbuhan Bibit Tanaman Manggis (Garcinia mangostana L.) Setelah Inokulasi dengan Berbagai Galur Agrobacterium rhizogenes1 Lizawati, ,; Poerwanto, Roedhy; Sobir, ,; Rusmana, Iman; Ermayanti, Tri Muji
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 2 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.098 KB) | DOI: 10.24831/jai.v35i2.1321

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Growth of mangosteen essentially depends on its root system.  Therefore, it needs technology to obtain stringer mangosteen root system.  The use of Agrobacterium rhizogenes bacterium is an alternative.  The objectives of this experiment were : 1) to find the effective strain of A. rhizogenes bacterium for inoculation of mangosteen seedling root, 2) to find the best inoculation method for inducing mangosteen seedling root.  The materials used in this experiment were ; mangosteen fruit and A. rhizogenes collection from Puslit Biotechnology LIPI Cibinong-Bogor.  The experiment was arranged in completely randomized design with two factorial treatments.  The first factor : 11 strains A. rhizogenes (ATCC-15834, ATCC-8196, R-1000, 07-20001, A4, A4-J, 509, 510, 511, MAFF 01-1724, and control), the second factor : 2 inoculation methods (cutting and dipping).  The results showed that A. rhizogenes  of ATCC-15834, 509, 07-20001, A4, and R-1000 increased stem diameter, plant height, leaf number, lateral and tertiary root number, better than ATCC-8196, MAFF 01-1724, 510, 511, A4-J, and control.  Cutting root method of inoculation resulted in higher live plant percentage compared to dipping root method.   Key words :  Agrobacterium rhizogenes, Garcinia mangostana, inoculation
Aktivitas Kitinase dan Peroksidase dari Ekstrak Protein Daun, Akar, Kalus dan Tunas In Vitro Trichosanthes tricuspidata Lour. Sukma, Dewi; Poerwanto, Roedhy; Sudarsono, ,; Khumaida, Nurul; Wiyono, Suryo; Artika, I Made
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 1 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.991 KB) | DOI: 10.24831/jai.v36i1.1347

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A number of Trichosanthes species has been reported as a source of bioactive protein associated with defense mechanisms such as chitinase. Chitinase and peroxidase of crude protein extracted from leaves, roots, in vitro calli and shoots of T. tricuspidata had been analysed. Calli were induced on MS medium containing combinations of 1 µM NAA + 1 µM BA (K1), 2 µM NAA + 2 µM BA (K2), 3 µM NAA + 3 µM BA (K3), or 4 µM NAA + 4 µM BA (K4). Shoots were cultured in MS with 1 mg/l of BA, while leaves and roots were harvested from six-month old plants grown on the field. Results of the experiment suggested that K1-K4 medium could be used to induce calli although weight of calli from all medium composition was not significantly different (0.19-0.31 g/explant/4 weeks). Calli from K1 medium had the highest of total crude protein content (3.24 mg/ml). The highest of chitinase activity was found in in vitro shoots (6.51 mM pNP/hour/mg protein) and the highest peroxidase activity was in the plant roots (0.25 ∆ 420/minute/mg protein). Key words: in vitro calli, shoots, crude protein, chitinase and peroxidase activities
Pola Kerontokan Buah Tiga Kultivar Mangga Sakhidin, ,; Purwoko, Bambang S .; Poerwanto, Roedhy; Susanto, Slamet; Yahya, Sudirman; Abidin, Ahmad S.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 32 No. 2 (2004): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1018.035 KB) | DOI: 10.24831/jai.v32i2.1436

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Information on the pattern of fruit drop of mango is required to determine the appropriate method and time in reducing fruit drop. By this methodf, fruit retention or number of harvested fruit of mango can be increased.The aim of this research was to determine the pattern of fruit drop of Gadung 21, Manalagi 69 and Golek 3 I . The results of this researchs howedt hat the pattern of fruit drop of Gadung2 1, Golek 31 and Manalagi 69 was similar. All cultivars I showed that there was one peak (the highest number of fruit drop) in fruit drop. It occurred at 6 days after anthesis (DAA). After 24 DAA, the number offruit drop was constant, namely near to zero. It occurred until harvest.Key words: Mango, Fruit drop, Fruit set
Studies on Dormancy Periods and Growth Rhythm of Shoot and Root of Mangosteen (Garcinia mangostana L.) Hidayat, Ramdan; Surkati, Achmad; Poerwanto, Roedhy; Darusman, Latifah K.; Purwoko, Bambang S.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 2 (2005): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.163 KB) | DOI: 10.24831/jai.v33i2.1516

Abstract

Mangosteen has a good prospectus for international market. Many people in the world like this fruit because it is delicious, has high nutrient contents, and it can be consumed as a fresh fruit. The growth of mangosteen is very slow with a very long juvenile period because of its long dormancy period. The objective of this research was to study the dormancy periods and growth rhytm of shoot and root of young and adult mangosteen seedlings. The research used completely randomized design and consisted of two experiments. The first experiment was to study the dormancy and flushing periods. The second experiment was to study the growth patterns of shoot and root foom several ages of mangosteen seedlings. This research was conducted at Mekarsari Fruit Park, Cileungsi, Bogor. The result of the experiment showed that two years old of mangosteen seedling had 5 flushes per year, four years old of mangosteen had 3-4 flushes per year, and eight years old of mangosteen only had 2 flushes. Dormancy periods were significantly different between 2,4 and 8 year of mangosteen seedling. After the seedlings have branches, the dormancy periods become twice as many as that before branching. The roots grew rapidly two weeks before flush and slower after flush occurred.   Key words: Garcinia mangostana, dormancy, flush, seedling
Co-Authors , Dorly , Sakhidin , Yudiwanti . DARMAWAN . SAMANHUDI . SUHARSONO Abdul Munif Achmad Surkati Ade Wachjar Agus Purwito Ahmad Ghozi Manshuri Ahmad Ghozi Mansyuri Ahmad S. Abidin Ahmad Sutopo Akmal, Ajmir ALI NURMANSYAH Alifiya Herwitarahman Anas D Susila Anas Dinurrohman Susila Andria Agusta ANDRIA AGUSTA Ansyori, Ansyori Aris Purwanto Asmini Budiani Bambang S . Purwoko Bambang S. Purwoko Bambang Sapta Purwoko Cenra Intan Hartuti Tuharea CICIK SURIANI D Fatria D Fatria Deden Derajat Matra Defitrianida, Asyhuriyah Wardah Dewi Sukma Dhika Prita Hapsari Dian Fahrianty Didy Sopandie Djoko Santoso Dorly Dorly E Efendi Edi Santosa Edi Santosa Edi Santosa Efendi, Darda EKO SETIAWAN Eko Setiawan Endah Retno Palupi Endang Gunawan Faqih Udin Fauziyah Harahap Fumio Fukuda HAJRIAL ASWIDINNOOR Hanifah Muthmainnah Hartrisari Hardjomidjojo Hiroshi Inoue I Hidayati I MADE ARTIKA I NYOMAN RAI Ilmi, Nadhirah Karimatul Iman Rusmana Inanpi Hidayati Sumiasih, Inanpi Hidayati Indah Wulandari INDAH WULANDARI Irsyad Maulana Iskandar Lubis Jawal Muhammad Anwarudinsyah Juanasri Juanasri JULIARNI JULIARNI Kasutjianingati . Ketty Suketi Kuniyuki Saitoh La Ode Safuan Laksono Trisnantoro Latifah K. Darusman Latifah Kosim Darusman Lizawati . Lubis, Wahyu Muhammad Yuha Lukman Liferdi Machfud Machfud Marimin Marimin Matra, Deden Derajat Maulana, Mohamad Akhbar Memen Surahman Mohamad Akhbar Maulana Mohamad Rahmad Suhartanto Muhamad Noor Azizu, Muhamad Noor Muhammad Arif Nasution Muhammad Darmawan Musdalifah, Nuzlul Naohiro Kubota Nian Rimayanti H. Nobuo Sugiyama Nono Sutrisno Nono Sutrisno Nur Wahyu Sariningtias Nurfitri Ramadhani Nurul Khumaida Odit Ferry Kurniadinata Qadir, Abdul Rahayu, Resa Sri Rahmat Budiarto Ramdan Hidayat Rd. Selvy Handayani Resa Sri Rahayu Resa Sri Rahayu Retno Astuti Riana Jumawati Rofiq, Muhamad Abdul Roza Yunita S Susanto Santun R.P. Sitorus Septirosya, Tiara Slamet Susanto Soaloon Sinaga Sobir Sobir SOEKISMAN TJITROSEMITO Sri Astuti Rais Sri Yuliani Sri Yuliani Sriani Sujiprihati SRIANI SUJIPRIHATI Suci Rahayu Sudarsono Sudirman Yahya Suryo Wiyono Sutrisno, Sutrisno T Purnama Tanari, Yulinda Taruna Shafa Arzam Taruna Shafa Arzam, Taruna Shafa TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tetty CHAIDAMSARI Tiara, Dede Titin Purnama Tri Muji Ermayanti Trikoesoemaningtyas Vandra Kurniawan Wa Ode Muliastuty Widya Sari Winarso D. Widodo Y A Purwanto Y A Purwanto Yandra Arkeman Yundari, Yundari Yunus, Ismadi