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Studi Akumulasi Pigmen β-Cryptoxanthin untuk Membentuk Warna Jingga Buah Jeruk di Daerah Tropika Sumiasih, Inanpi Hidayati; Arzam, Taruna Shafa; Poerwanto, Roedhy; Efendi, Darda; Agusta, Andria; Yuliani, Sri
Jurnal Hortikultura Indonesia (JHI) Vol. 9 No. 2 (2018): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (934.285 KB) | DOI: 10.29244/jhi.9.2.73-83

Abstract

ABSTRACTDegreening is a transformation process on peel which enables it to change color from green to orange on citrus fruits. The orange color of the peel comes from the mixture of carotenoid pigments, such as β-cryptoxanthin and β-citraurin. The pigments contributed in the formation of β-citraurin are β-cryptoxanthin and zeaxanthin. The objectives of this study were (1) to obtain proper degreening temperature in the orange color formation of several citrus varieties, and (2) to identify and determine pigments of β-cryptoxanthin pigment and total chlorophyll content in citrus peel after degreening. This study was conducted at PKHT IPB and LIPI Cibinong from July 2013 to December 2013, and from February 2016 to May 2017. About 100 ppm of ethylene gas was injected into a citrus-containing box using 5 ml syringe, then the box was placed in cool storage at 15 0C, 20 0C and room temperature, for 72 hours. The results showed that the best colors of Keprok Selayar and Keprok Tejakula were obtained by the degreening at 15 0C, in Siam Kintamani it was obtained by degreening at 20 0C. Degreening significantly reduced the total chlorophyll content, and increased β-cryptoxanthin content. The content of β-cryptoxanthin after degreening was 3 folds higher on highland Citrus reticulata than lowland citrus.Keywords: citrus color index, chlorophill, degreening, ethylene, tropical citrusABSTRAKDegreening adalah proses perombakan warna hijau pada kulit jeruk diikuti dengan proses pembentukan warna jingga. Warna jingga adalah campuran antara β-cryptoxanthin dengan β-citraurin. Pigmen yang berkontribusi dalam pembentukan β-citraurin adalah β-cryptoxanthin dan zeaxanthin. Tujuan penelitian ini ialah (1) Mendapatkan suhu degreening yang tepat dalam pembentukan warna jingga pada beberapa varietas jeruk, (2) Identifikasi dan penentuan kadar pigmen β-cryptoxanthin dan kandungan total klorofil pada kulit jeruk setelah degreening. Penelitian ini dilakukan di PKHT IPB dan LIPI Cibinong pada bulan Juli 2013 sampai Desember 2013, dan bulan Februari 2016 sampai Mei 2017. Degreening dilakukan dengan menginjeksikan gas etilen konsentrasi 100 ppm ke dalam wadah tertutup yang berisi jeruk menggunakan syringe 5 ml, kemudian disimpan pada suhu 15 0C, 20 0C dan suhu ruang, selama 72 jam. Hasil penelitian menunjukkan bahwa warna terbaik jeruk Keprok Selayar dan Tejakula diperoleh dengan degreening pada suhu 15 0C, Siam Kintamani diperoleh dengan degreening pada suhu 20 0C. Degreening dapat menurunkan kandungan total klorofil secara tajam, dan terbukti meningkatkan kandungan pigmen β-cryptoxanthin. Kandungan pigmen β-cryptoxanthin setelah degreening 3 kali lebih tinggi pada jeruk keprok dataran tinggi dibandingkan dengan dataran rendah.Kata kunci: citrus color index, degreening, etilen, jeruk tropika, klorofil
Appropriate Duration of Drought Stress for Madura Tangerine Flower Induction: Appropriate Duration of Drought Stress for Madura Tangerine Flower Induction Rahayu, Resa Sri; Poerwanto, Roedhy; Efendi, Darda; Widodo, Winarso Drajad
Jurnal Hortikultura Indonesia (JHI) Vol. 11 No. 2 (2020): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.11.2.82-90

Abstract

Cekaman kekeringan merupakan salah satu teknik yang dilakukan untuk menginduksi bunga jeruk keprok di luar musim dalam upaya memenuhi ketersediaan buah jeruk keprok sepanjang tahun. Durasi cekaman kekeringan yang tepat penting dipelajari untuk menghindari cekaman berat dan mendapatkan hasil bunga yang optimum. Penelitian ini bertujuan menentukan durasi cekaman kekeringan yang tepat untuk menginduksi bunga jeruk keprok dataran rendah varietas Madura. Penelitian dilaksanakan di Kebun Percobaan PKHT-IPB dengan ketinggian ± 300 mdpl dari bulan April-Mei 2019. Percobaan dirancang dengan RAK (Rancangan Acak Kelompok) dengan satu faktor perlakuan yaitu durasi cekaman kekeringan dengan lima taraf: tanpa cekaman kekeringan sebagai kontrol, durasi cekaman kekeringan satu minggu, durasi cekaman kekeringan dua minggu, durasi cekaman kekeringan tiga minggu dan durasi cekaman kekeringan empat minggu. Hasil penelitian menunjukkan bahwa durasi cekaman kekeringan mempengaruhi keberhasilan induksi bunga jeruk keprok Madura. Durasi cekaman kekeringan selama 2, 3 dan 4 minggu dengan nilai kadar air secara berturut-turut 81.81%, 65.21% dan 55.39% dari kapasitas lapang berhasil menginduksi bunga. Bunga muncul pada 3, 2 dan 1 minggu setelah rewatering dan pengairan rutin secara berturut-turut pada perlakuan durasi cekaman 2, 3 dan 4 minggu. Tiga minggu tanpa irigasi merupakan durasi cekaman kekeringan optimum untuk induksi bunga jeruk keprok Madura.
Respons fisiologi dan profile metabolit sekunder Lima Kultivar Rambutan (Nephelium lappaceum L.) terhadap Penambahan Cahaya Buatan LED (Light Emitting Diode): Physiology and Secondary Metabolite Profiles Responses of Five Rambutan Varieties (Nephelium lappaceum L.) through Addition of Artificial Light LED (Light Emitting Diode) Irsyad Maulana; Matra, Deden Derajat; Roedhy Poerwanto
Jurnal Hortikultura Indonesia (JHI) Vol. 13 No. 3 (2022): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.13.3.180-186

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Rambutan (Nephelium lappaceum L.) is a fruit that is suitable for growing in the tropics area. However, information on physiological responses and secondary metabolite profiles on artificial light treatment has not been reported. This study aims to study the response of rambutan plants to the application of artificial light from LED (Light Emitting Diode). The study was conducted in the screenhouse nursery using a two-factor nested plot design, namely LED as light source and cultivar. The LED light sources used red, blue, and white with application duration for 21 hours while the cultivars used Sikoneng, Parakan, Cilebak, Gravel, and Garuda cultivars. The results showed that rambutan plants that were given additional blue LEDs contained higher chlorophyll and leaf as glucose and fructose compared to another treatments. The results of the secondary metabolite profiles showed the largest Neophytadiene compound found in the Parakan cultivar under LED red treatment. Meanwhile, the compound that has the greatest abundance in white LEDs is 1-Propene, 3-[(4-nitrobutyl)thio]. Keywords: chlorophyll, photosynthesis, glucose, light spectrum, secondary metabolite
Sensory Evaluation of the Quality of Kaffir Lime (Citrus hystrix DC.) Leaves Exposed to Different Postharvest Treatments Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efedi, Darda; Agusta, Andria
Journal of Tropical Crop Science Vol. 8 No. 02 (2021): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.8.02.71-79

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This study aimed to evaluate the sensory attributes such as aroma, color and texture of kaffir lime leaves in response to various post-harvest treatment. The hedonic test approach was conducted by inviting 70 untrained panelists on seven post-harvest treatments, i.e. (D1) post-sortation fresh leaves, (D2) pre-sortation fresh leaves, (D3) cold-storage leaves, (D4) low temperature-storage leaves, (D5) brown dry leaves, (D6) fresh leaf-flour, and (D7) brown dry leaf-flour. The result showed that most of panelist agreed that aroma was the most important quality attribute that determined the level of preference in kaffir lime leaf products. Among seven tested products, the aroma, color, texture and overall impression of D1 was the most favorite one, while D4, D5, and D7 were assessed as low preference products. This work showed the importance of sortation and cold storage to maintain consumer likeliness. The criteria for sortation were green, clean, pest-disease free, scar free, high uniformity and fresh condition of leaves. Cold-storage at -20oC maintained the color and texture of kaffir lime leaves better than low temperature storage (5oC). To make leaf flour, the use of fresh green leaves was significantly better than brown dry ones. This finding might become the baseline data for the development of kaffir lime leaf product in the future.
The Effects of Preharvest Mild Shading on the Quality and Production of Essential Oil from Kaffir Lime Leaves (Citrus hystrix) Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efendi, Darda; Agusta, Andria
Journal of Tropical Crop Science Vol. 9 No. 01 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.01.15-21

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Kaffir lime (Citrus hystrix DC) is a less popular citrus species commonly used as a food spice and a source of essential oil. Early studies report the success of preharvest mild shading to increase leaf yield, although there is still limited information on the effect of preharvest shading on the quality of essential oil produced. The aim of this current study is to evaluate the effect of preharvest mild shading factors on the yield, physical characteristics, and metabolite fingerprinting of kaffir lime leaves essential oil (KLLEO). One-year-old kaffir lime trees were sampled in two preharvest treatments, i.e., open sun and mild shading (24% light reduction) at Pasir Kuda experimental field, Bogor, Indonesia. Statistical analysis showed that there was no significant effect of preharvest treatment on yield and physical characteristics (color, specific gravity, and refractive index) of KLLEO. In contrast, there was a metabolite fingerprinting variation of KLLEO as an effect of mild shading. The relative percentage of bergamol, citronellol, caryophyllene oxide, citronellic acid, isopulegol, isopulegyl formate, limonene, linalool, and linalool oxide was increased by mild shading. On the other hand, the main metabolite (citronellal) was significantly reduced by about 10% in shading treatment, as compared to the open-sun ones.
Yield and Physicochemical Characteristics of Kaffir Lime Leaf Essential Oils Subjected to Different Post-Harvest Treatment Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efendi, Darda; Agusta, Andria; Rofiq, Muhamad Abdul
Journal of Tropical Crop Science Vol. 11 No. 02 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.02.97-104

Abstract

The importance of kaffir lime leaf as essential oils (EOs) raw material is starting to get attention because of its commercial value; however, there is no quality reference for kaffir lime leaf EOs, especially in response to various post-harvest handlings. This study aimed to describe the physicochemical characteristics and yield of kaffir lime EOs subjected to different post-harvest. Bogor originated-kaffir lime leaf was prepared to be subjected to several post-harvest treatments, i.e., control/fresh green leaves (P1); milling to produce green leaf flour (P2); drying to produce dry brown leaf (P3), and milling and drying to produce brown leaf flour (P4). The result showed that post-harvest treatment generally decreases an oil yield and increases darkness color, specific gravity and refractive index of tested EOs. Additionally, post-harvest treatment also changes metabolite profile revealed by GCMS analysis. The relative percentage of caryophyllene and citronellol tends to increase, while the linalool and citronellal levels decrease due to tested post-harvest treatment. It was implied that for the benefit of the fragrance industry with a high citronellal requirement, EOs should be made from fresh green leaves and the leaves should be avoided from drying and powdering treatment.
We investigated the effect of sector (position in canopy) on translocation and distribution of 13C-photosynthates in  mangosteen trees and related the findings to previous analyses of fruit quality. Our experiment was conducted on three 25-year-old mangosteen trees. Tree canopies were divided into 9 sectors based on height (bottom, middle, top) and width (inner, center, outer). One branch from each sector was labeled with 13CO2 in December 2003. Immediately after labeling, 13C concentration in l Setiawan, Eko; Poerwanto, Roedhy; Fukuda, Fumio; Sugiyama, Nobuo; Saitoh, Kuniyuki; Kubota, Naohiro
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 40 No. 2 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v40i2.6375

Abstract

We investigated the effect of sector (position in canopy) on translocation and distribution of 13C-photosynthates in  mangosteen trees and related the findings to previous analyses of fruit quality. Our experiment was conducted on three 25-year-old mangosteen trees. Tree canopies were divided into 9 sectors based on height (bottom, middle, top) and width (inner, center, outer). One branch from each sector was labeled with 13CO2 in December 2003. Immediately after labeling, 13C concentration in leaves from middle sectors was higher than that in leaves from other positions. 13C concentration in all leaves decreased rapidly for 24 h after 13C feeding, followed by a gradual decrease. In contrast, 13C concentration increased over time in the pericarp and aril of fruits. Translocation of 13C-photosynthates into fruit was high in Sectors 4 and 5, and in top positions (Sectors 7 to 9). At 96 h after 13C feeding, the highest distribution ratio of 13C-photosynthates was observed in stems, followed in descending order by pericarp, leaf, and aril. 13C distribution ratio in the aril was generally highest in fruits from inner and center positions. The relationship between partitioning of photosynthates and quality of mangosteen fruit, which differs among sectors, has been discussed. Keywords: fruiting positions, Garcinia mangostanaL., photosynthate partitioning, 13C, tree branches
Aktivitas Kitinase dan Peroksidase dari Ekstrak Kasar Protein Asal Kalus dan Berbagai Jaringan Tanaman Trichosanthes cucumerina var. anguina Sukma, Dewi; Poerwanto, Roedhy; Sudarsono, ,; Khumaida, Nurul; Artika, I Made; Wiyono, Suryo
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 40 No. 3 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.045 KB) | DOI: 10.24831/jai.v40i3.6830

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Chitinase and peroxydase are important bioactive proteins or are specific enzymes that are related to plant resistance to pathogens. The aims of the research were to analyze the chitinase and peroxidase activities of crude protein extract from calli, stem, leaves and roots of T. cucumerinavar. anguina. In the first experiment, chitinase and peroxydase activities were analyzed from in vitro calli, leaves and roots obtained from 2-month-old of field grown plants. The media for calli induction were Murashige and Skoog medium with addition of 1 µM NAA + 1 µM BA, 2 µM NAA + 2 µM BA, 3 µM NAA + 3 µM BA, or 4 µM NAA + 4 µM BA. In the second experiment, the chitinase and peroxydase activities from crude protein extract of roots, stems and leaves were analyzed. The extracts were from 3-week-old seedling (less than a month), 1-month and 2-month-old plants. The first and the second experiment results showed that crude protein extracts of plant roots from the field grown plants had the highest chitinase and peroxidase activities. Stem of field grown plants had the similar level of chitinase activities with the plant roots. Chitinase activities of in vitro calli were not significantly different from those of plant roots so that it could be used as an alternative for plant roots in studying chitinase from T. cucumerinavar. anguina. Chitinase activities in crude protein extracts of roots appeared constant whereas peroxidase tend to increase with plant age. Keywords: calli, enzyme activities, leaves, roots, stem
Pengaruh Batang Bawah dan Jenis Tunas pada Mikrografting Manggis (Garcinia mangostana) secara In Vitro Handayani, Rd. Selvy; Poerwanto, Roedhy; Sobir, ,; Purwito, Agus; Ermayanti, Tri Muji
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 1 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.152 KB) | DOI: 10.24831/jai.v41i1.7076

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The aim of this study was to investigate the effect of rootstock and shoot types on in vitro mangosteen micrografting.The experiment was arranged in completely randomized design (CRD) with two factors. The first factor was the rootstocktype, i.e. rooted planlet from the germination of quartered seed, and rooted planlet from the germination of undivided seeds.The second factor was the developmental phase of scion, i.e. dormant buds, and flush (had new leaf more than 2-4 mm). Theresults showed that rootstock derived from the germination of undivided seed had a higher success rate than other treatmentson all variables, except for number of new leaves. The use of flush as scion was better than dormant buds; flush resulted in ahigher percentage of successful micrograft and longer shoots. In vitro micrografting had a better growth rate than grafting at the same age. The results of anatomical observation conducted at four months after micrografting demonstrated that there was a good graft union, indicated by excellent fusion between rootstock and scion xylem tissues.Keywords: flush, in vitro, micrografting, rootstock, scion
Respon Fisiologi pada Pembibitan Mangga Kasturi (Mangifera casturi Kosterm) terhadap Aplikasi Cahaya LED (Light Emitting Diode) Herwitarahman, Alifiya; Poerwanto, Roedhy; Sopandie, Didy; Matra, Deden Derajat
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 49 No. 3 (2021): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.939 KB) | DOI: 10.24831/jai.v49i3.38250

Abstract

Mangga Kasturi (Mangifera casturi Kosterm) merupakan mangga endemik Kalimantan Selatan dengan status langka di alamnya berdasarkan IUCN-red list. Kasturi dengan masa juvenil yang panjang sehingga menyebabkan awal pembuahan yang lama. Penggunaan cahaya buatan dengan lampu LED-light emmiting diode diketahui dapat memperpendek juvenilitas karena meningkatkan efisiensi fotosintesis. Penelitian ini bertujuan untuk mempelajari perbedaan respon fisiologi dari bibit mangga Kasturi menggunakan lampu LED. Penelitian ini menggunakan rancangan petak tersarang dengan faktor utama lampu LED dan faktor kedua aksesi sebagai perlakuan yang disarangkan. Faktor utama terdiri dari tiga taraf yaitu LED merah, putih dan biru, sedangkan faktor kedua terdapat tiga taraf yaitu aksesi Cuban, Pelipisan, dan Kasturi. Penggunaan LED memberikan beberapa perbedaan respon terhadap kandungan klorofil, LED merah menunjukan nilai paling tinggi pada kandungan klorofil a, tetapi tidak ada pengaruh pada klorofil b dan rasio klorofil. Penyinaran LED biru menunjukkan nilai absorsi cahaya, konduktansi stomata, transpirasi, dan laju fotosintesis yang lebih tinggi pada aksesi Kasturi. Lampu LED biru meningkatkan kandungan glukosa, sedangkan lampu LED putih membuat kandungan fruktosa yang lebih tinggi dibandingkan lampu lain dan mempengaruhi ketebalan daun yang lebih tebal. Berdasarkan penelitian ini monokromatik LED memberikan pengaruh berbeda pada setiap karakter fisiologis, yang berpengaruh pada percepatan masa juvenil. Kata kunci: fotosintesis, fruktosa, glukosa, rasio klorofil
Co-Authors , Sakhidin , Yudiwanti , Yudiwanti . DARMAWAN . SAMANHUDI . SUHARSONO Abdul Munif Achmad Surkati Ade Wachjar Agus Purwito Ahmad Ghozi Manshuri Ahmad Ghozi Mansyuri Ahmad S. Abidin Ahmad Sutopo Akmal, Ajmir ALI NURMANSYAH Alifiya Herwitarahman Anas D Susila Anas Dinurrohman Susila Andria Agusta ANDRIA AGUSTA Ansyori, Ansyori Aris Purwanto Asmini Budiani Bambang S . Purwoko Bambang S. Purwoko Bambang Sapta Purwoko Cenra Intan Hartuti Tuharea CICIK SURIANI D Fatria D Fatria Deden Derajat Matra Defitrianida, Asyhuriyah Wardah Dewi Sukma Dhika Prita Hapsari Dian Fahrianty Didy Sopandie Djoko Santoso Dorly Dorly E Efendi Edi Santosa Edi Santosa Edi Santosa Efendi, Darda EKO SETIAWAN Eko Setiawan Endah Retno Palupi Endang Gunawan Faqih Udin Fauziyah Harahap Fumio Fukuda HAJRIAL ASWIDINNOOR Hanifah Muthmainnah Hartrisari Hardjomidjojo Hiroshi Inoue I Hidayati I MADE ARTIKA I NYOMAN RAI Ilmi, Nadhirah Karimatul Iman Rusmana Inanpi Hidayati Sumiasih, Inanpi Hidayati Indah Wulandari INDAH WULANDARI Irsyad Maulana Iskandar Lubis Jawal Muhammad Anwarudinsyah Juanasri Juanasri JULIARNI JULIARNI Kasutjianingati . Ketty Suketi Kuniyuki Saitoh La Ode Safuan La Ode Safuan Laksono Trisnantoro Latifah K. Darusman Latifah Kosim Darusman Lizawati . Lubis, Wahyu Muhammad Yuha Lukman Liferdi Machfud Machfud Marimin Marimin Matra, Deden Derajat Maulana, Mohamad Akhbar Memen Surahman Mohamad Akhbar Maulana Mohamad Rahmad Suhartanto Muhamad Noor Azizu, Muhamad Noor Muhammad Arif Nasution Muhammad Darmawan Musdalifah, Nuzlul Naohiro Kubota Nian Rimayanti H. Nobuo Sugiyama Nono Sutrisno Nono Sutrisno Nur Wahyu Sariningtias Nurfitri Ramadhani Nurul Khumaida Nurul Khumaida Odit Ferry Kurniadinata Qadir, Abdul Rahayu, Resa Sri Rahmat Budiarto Ramdan Hidayat Rd. Selvy Handayani Resa Sri Rahayu Resa Sri Rahayu Retno Astuti Riana Jumawati Rofiq, Muhamad Abdul Roza Yunita S Susanto Santun R.P. Sitorus Septirosya, Tiara Slamet Susanto Soaloon Sinaga Sobir Sobir SOEKISMAN TJITROSEMITO Soekisman Tjitrosemito Sri Astuti Rais Sri Astuti Rais Sri Yuliani Sri Yuliani Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Suci Rahayu Sudarsono Sudirman Yahya Suryo Wiyono Sutrisno, Sutrisno T Purnama Tanari, Yulinda Taruna Shafa Arzam Taruna Shafa Arzam, Taruna Shafa TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tetty CHAIDAMSARI Tiara, Dede Titin Purnama Tri Muji Ermayanti Tri Muji Ermayanti Trikoesoemaningtyas Vandra Kurniawan Wa Ode Muliastuty Widya Sari Winarso D. Widodo Y A Purwanto Y A Purwanto Yandra Arkeman Yundari, Yundari Yunus, Ismadi