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Djoko Agus Purwanto
Departemen Ilmu Kefarmasian, Fakultas Farmasi Universitas Airlangga, Indonesia
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Analisis Kadar O6 -[H3 ]Metilguanin-DNA pada Kultur Hepatosit Tikus Menggunakan Liquid Scintillation Counter Setelah Pemberian (-)-Epigalokatekin Galat dari Teh Hijau Purwanto, Djoko Agus
Jurnal Kedokteran Indonesia Vol 2, No 1 (2011)
Publisher : Jurnal Kedokteran Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (31.974 KB)

Abstract

Background: Alkylation of DNA can occur at various site, however alkylation of the O6-position ofguanine has the strongest mutagenic potential. O6-Methylguanine is a toxic and mutagenic lesionwhich is formed in cellular DNA by alkylating agents. Alkyl group from carbanium ion can attack DNAforms alkylated DNA especially at O6 position of guanine. O6-methylguanine mispairs with thymineduring replication, and if the adduct is not removed, this results in conversion from a guanine-cytosinepair to an adenine-thymine pair. Deficient repair of O6-methylguanine has been suggested to be acontributory factor in the etiology of some diseases such as diabetes and cancer. This study aimed todetermine O6-methylguanine DNA concentration induced by N-[H3]methyl-N-nitrosourea ([H3]MNU)in rat liver cell culture with and without (-)-epigalocatechin gallate exposure.Methods: Primary rat liver cell culture was divided into 10 groups. Five groups for [H3]MNU 32 μMexposure, and 5 groups remain for 48 μM [H3]MNU exposure. Each group of [H3]MNU exposure gotEGCG 0 (as control), 8, 17, 33 and 67 ppm. The DNA was isolated, and O6-[H3]methylguanine DNAseparated by high performance liquid chromatography (HPLC). Concentration of O6-[H3]methylguanineDNA was measured by Liquid Scintillation Counter.Results: O6-Methylguanine DNA concentration decreased significantly in liver cell culture groups withEGCG. O6-[H3]Methylguanine DNA concentration in control groups for [H3]MNU 32 μM and 48 μMwere 29.89 ± 2.01 and 32.00 ± 1.67 fmol/μg DNA, respectively, while with EGCG 67 ppm were 1.87 ± 0.94and 2.38 ± 0.36 fmol/μg DNA.Conclusion: O6-methylguanine DNA concentration in liver rat culture induced by MNU decreasessignificantly after EGCG exposure. These findings support the conclusion that EGCG plays a key role insuppressing and inhibiting cancer development.Keywords:(-)-epigalocatechin gallate, N-[H3]methyl-N-nitrosourea, O6-methylguanine DNA, LiquidScintilation Counter
Implementing Health Safety Environment (HSE) Process Management to Improve HSE Performance, Competitive Advantage and Financial Performance Purwanto, Agustinus Hariadi DJoko; Suharjo, Budi; Sumarwan, Ujang; Daryanto, Heny K
Jurnal Manajemen Teknologi Vol 13, No 1 (2014)
Publisher : SBM ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12695/jmt.2014.13.1.5

Abstract

Abstract. Performance of health safety environmental (HSE) has been tightly demanded by stakeholders (customer, government, public, NGO, employee) and become essential factors of organization’s competitive advantages. High HSE performance leads to improving customer satisfaction, legitimacy and reputation from which better financial performance can be gained. In order to do that, companies must implemement HSE process management. The research aims to investigate effects of the four constructs, namely process management, HSE performance, organization’s competitive advantage and financial performance. The study uses descriptive and quantitative statistical methods involving 119 companies of which SEM is used to see linkages between factors. The testing results show that the process management gives significant positive effect to HSE performance after which it simulataneously affect significant and positively to competitive advantage but it has no significant relationship with financial performance. However, competitive advantage affect positively to financial performance. In conclusion, HSE process management improves HSE performance, which subsequently increases Competitive advantage but it does not affect financial performance. The increase of financial performance is given by competitive advantage.Key words: HSE, Performance, Competitive Advantages, Financial Performance, SEM Abstrak. Kinerja lingkungan dan keselamatan dan kesehatan kerja (LK3) telah dituntut dengan ketat oleh para pemangku kepentingan (pelanggan, pemerintah, masyarakat, LSM, karyawan) dan menjadi faktor-faktor esensial bagi keunggulan kompetitif organisasi. Untuk itu, perusahaan harus menerapkan manajemen proses LK3. Kinerja LK3 tinggi memberikan kepuasan pelanggan, legitimasi dan reputasi sehingga kinerja finansial dapat diperoleh. Penelitian ini bertujuan untuk mengamati pengaruh-pengaruh empat konstruk, yaitu Manajemen proses LK3, Kinerja LK3, Keunggulan kompetitif dan Kinerja finansial. Penelitian menggunakan metode analisa deskriptif dan kuantitatif yang meliputi 119 perusahaan dimana SEM diterapkan untuk melihat hubungan-hubungan antar faktor secara simultan. Hasil penelitian menunjukkan bahwa Manajemen proses LK3 memberikan pengaruh positif terhadap Kinerja LK3 yang selanjutnya secara simultan memberikan pengaruh positif terhadap Keunggulan kompetitif dan tidak memiliki pengaruh signifikan terhadap Kinerja finansial. Tetapi, Keunggulan kompetitif memberikan pengaruh positif terhadap Kinerja financial. Kesimpulan yang diambil adalah penerapan manajemen proses LK3 meningkatkan Kinerja LK3 yang selanjutnya meningkatkan Keunggulan kompetitif tetapi tidak mempengaruhi Kinerja finansial. Peningkatan kinerja finansial diberikan oleh keunggulan kompetitif.Kata kunci: LK3, Kinerja, Keunggulan Kompetitif, Kinerja Finansial, SEM 
Molecular Docking, Pharmacokinetics, and Toxicity Prediction of Epigallocatechin-3-Gallate (EGCG) on IKK Receptor in Photoaging Prevention Damayanti1,2, Cita Rosita Sigit Prsakoeswa2, Djoko Agus Purwanto3, Anang Endaryanto4, Siswandono3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.3131

Abstract

Photoaging is skin aging, caused by chronic exposure of ultraviolet radiation. Photoaging decreases patients’ quality of life because the skin was the outer organ seen by others. Ultraviolet radiation causes oxidative stress, that activated inhibitory kappa B kinase (IKK), increased nuclear factor kappa B (NF-kB), matrix metalloproteinase (MMP), and degradation of collagen. Epigallocatechin-3-gallate (EGCG) was the main green tea polyphenol and the main source of biologic activity of green tea. This study was an in silico study, aimed to obtain the effectiveness of EGCG component through molecular docking on IKK receptor (PDB ID: 5EBZ). The bioinformatics tools based on reverse docking used in this study, were Protein Data Bank, ChemDraw, Chem3D, and Molegro Virtual Docker software. Docking and binding site analysis showed, that EGCG was able to interact with IKK receptor. Rerank score of interaction between EGCG and IKK receptor was higher than that of arbutin and 5TL_701[A]. It showed that EGCG has higher potential in photoaging prevention than arbutin, as one of the agents in photoaging prevention. Pharmacokinetics and toxicity (ADMET) prediction in this in silico study were conducted using pkCSM On Line tool. The pkCSM results showed EGCG was predicted having good pharmacokinetics profile without toxicity effect.
Potential of Chlorogenic Acid from Coffea canephora to Improving Innate Immunity System Components among BALB / c Mice Christina Destri1, Jusak Nugraha2, Muhammad Amin3, Djoko Agus Purwanto4
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 1 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i1.13915

Abstract

The aim of this study was to verify the phagocytosis activity which displayed in CD11b and B220 markersand also the markers of erythroid 2 nuclear factor related factor 2 (Nrf2) as a key regulatory transcriptionfactor on various antioxidant gene expressions and Superoxide dismutase (SOD) as an antioxidant markerthat related with protein. Nrf2 plays an important role to inhibit the ROS accumulation and eliminate freeradicals. The active compound of chlorogenic acid in robusta coffee type (Coffea canephora) in IndonesianCoffee and Cocoa Research Center was used in three groups of mice in this study and it was gave differentdose in each groups. The assessment samples were taken from the peritoneal fluid of mice than it wasanalyzed by using Flow Cytometry method to find phagocytosis function and antioxidant activity. Theresults of ANOVA statistical test was p <0.05 in all parameters, this mean that there was indicated that theactive compound of chlorogenic acid in coffee was involved in natural immune system mechanism and itwas seen in increasing of phagocytic activity and antioxidant levels.
In Vitro Culture Technology for the Production of Secondary Metabolites and the Development of Plants That are Resistant to Climate Change Sutini; Widiwurjani; Augustien, Nora; Guniarti; Pribadi, Didik Huswo; Purwanto, Djoko Agus; Muslihatin, Wirda
Nusantara Science and Technology Proceedings Seminar Nasional Magister Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2020.0604

Abstract

Climate change that we are experiencing today is triggered by global warming which causes air temperature to rise, drought-land degradation, fluctuating rainfall, floods, landslides, pollution, and rising sea levels. In the agricultural sector, this results in decreased soil fertility, decreased agricultural yields, physiological effects on plants, weed disturbances and attacks by pests. Therefore, the estimation strategy and the provision of adaptive technology include applying plant-invitro culture technology that can adapt to climate change. The problem of this paper is the decrease in productivity and the stagnation of secondary metabolite biomass production in plants. This problem can be overcome by adapting in vitro culture techniques to produce secondary metabolites and plant development. In vitro culture has advantages including it does not depend on climate, altitude, weather and environmental temperature. The aim of this study was to adapt the in vitro plant culture techniques to obtain secondary metabolites and adaptive plant development. The method used is a review of the literature related to in vitro culture techniques that can produce secondary metabolite biomass and the development of various plant varieties. The conclusion of this research is that in vitro culture techniques can produce a variety of secondary metabolites and adaptive plants. The implication of this paper is to obtain a variety of secondary metabolite biomass and various plants that are resistant to climate change.
Green Tea Suppresses Serum TNF-? and TGF-?1 Levels In Mice Model of Systemic Lupus Erythematosus Herin Mawarti; Jusak Nugraha; Djoko Agus Purwanto; Joewono Soeroso
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14746

Abstract

Objective: This study aims to investigate the effects of green tea administration on TNF-a, Hsp70, andTGF-b1 levels in the systemic lupus erythematosus (SLE). Material and methods: A total of 32 micewill be divided into four groups (each 8 mice), namely the control group, the SLE group, the SLE groupwho were given green tea extract at a dose of 500 mg/kg body weight, and the SLE group who were givengreen tea extract at a dose of 1000 mg/kg body weight. Analysis of TNF-a, Hsp70, and TGF-b1 levels wascarried out using the enzyme-linked immunosorbent assay technique. Results: TNF-a and TGF-b1 levelswere significantly increased in the SLE group compared to the control group (p < 0.05). This increase canbe significantly reduced through the provision of green tea, even reaching levels comparable to the controlgroup (p > 0.05). Conclusions: It was concluded that green tea containing EGCG can suppress TNF-a andTGF-b1 in the SLE model. Thus, green tea can be an alternative in immunology modulation in SLE.
Environmental Factors and Protective Effects of Epigallocatechin-3-Gallate to Systemic Lupus Eritematosus: A Review Study Herin Mawarti; Jusak Nugraha; Djoko Agus Purwanto; Joewono Soeroso
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14750

Abstract

SLE (Systemic Lupus Erythematosus) is one of autoimmune disease in which the immune system attacksits own tissues, causing widespread inflammation and tissue damage in the affected organs. Etiology andpathogenesis are not clearly mention until now by the scientist, but environmental factor is contribute todevelopment of Lupus and Flare disease. The objective of this study to explain the relationship betweenenvironmental factors and pathogenesis development of SLE, and how the Epigallocatechin-3-Gallate(EGCG) can protect or prevent of organs damage and environmental effects.Method: The research design of this study was used literature review. The data sources collected fromElectronic database in Pubmed, Sage, Google Scholar and website of science including reports, journals andmostly published in the last 10 years.Results: The pathomechanism of SLE was influenced by environmental factors and this was caused anincreasing of oxidative stress, chronic inflammation, dysregulation of immune system and decreased theclean of immune complex and apoptosis of cells. The production of autoantibodies and immune complex arerelated with target tissue with is it was caused chronic inflammation and the end it caused irreversible damagein glomerulus of the kidneys, arteries, lungs and other tissues. EGCG plays a protecting role in environmentalfactors as a trigger because it works as an antioxidant, anti-inflammatory and immunomudulator. So EGCGcan as a potential agent to protect SLE.Conclusion: Environmental factors plays an important role as a triger of SLE and flare. Moreover, EGCG asa potential agent to protect the presence of oxidative stress, inflammation and immune dysregulation.
The Dynamics of Macrophage Function in Reparation of Natural Immune System in Tuberculosis Disease Christina Destri; Jusak Nugraha; Muhammad Amin; Djoko Agus Purwanto
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15720

Abstract

Macrophages related with pathogen was involved the ability of Mycobacterium tuberculosis (Mtb) to developing mechanisms to prevent macrophage attacks and the aim of this study was to find the relationship between specific role of macrophages in natural immune system in tuberculosis (TB) disease. The research design of this study used literature reviews from various journals and it was accessed in Google Scholar and various medical science websites who bublessed less than 10 years. Localization of mycobacteria in granulomas form and activation of macrophages ability to kill and eliminate pathogens also the movement of M1 to M2 polarization as an important part for the host to prevent widespread tissue damage. However, the loss of the immune system suppression by M1 and Th1 molecules will give benefit to fertility development of Mtb in the environment of immunity M2 / Th2. The results of this study showed that the dynamics of macrophages in tuberculosis disease is a balance of Mtb’s ability to escape from the immune system. The effectiveness of anti-TB treatment repair the immune system and eliminate Mtb
HUBUNGAN ANTARA PENGETAHUAN IBU TENTANG IODIUM DENGAN KETERSEDIAAN GARAM BERIODIUM DI RUMAH TANGGA Yayuk Hartriyanti; Adi Utarini; Djoko Agus Purwanto; Budi Wikeko; Susetyowati Susetyowati; Toto Sudargo; A.Fahmy Arif Tsani
Media Gizi Mikro Indonesia Vol 13 No 1 (2021): Media Gizi Mikro Indonesia Edisi Desember 2021
Publisher : Balai Penelitian dan Pengembangan Kesehatan Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/mgmi.v13i1.4424

Abstract

Latar Belakang. Iodium merupakan mikronutrien penting terutama bagi perkembangan otak janin dan anak. Iodium berperan untuk proses pertumbuhan dan perkembangan sebagian besar organ terutama otak. Konsumsi iodium yang rendah dalam jangka panjang merupakan salah satu faktor yang dapat menyebabkan gangguan akibat kekurangan iodium (GAKI). Tingkat pengetahuan mengenai GAKI dan garam beriodium berpengaruh terhadap ketersediaan dan praktik penggunaan garam beriodium. Pemerintah telah mengupayakan penanggulangan GAKI melalui fortifikasi garam dengan iodium. Tujuan. Penelitian ini bertujuan untuk menganalisis pengetahuan ibu tentang iodium dengan ketersediaan garam beriodium di rumah tangga dan faktor yang memengaruhinya. Metode. Penelitian ini merupakan penelitian cross-sectional. Data diambil dari 198 rumah tangga menggunakan teknik proportional stratified random sampling. Penilaian pengetahuan ibu dilakukan dengan menggunakan kuesioner dalam bentuk pertanyaan tertutup. Sementara itu, penilaian ketersediaan garam diperoleh dengan pengujian kandungan iodium (KIO3). Uji statistik yang digunakan adalah chi-square test/fisher’s exact test dan Mann Whitney U/Kruskal Wallis untuk mengetahui hubungan antar variabel. Hasil. Sebagian besar responden tinggal di daerah dataran tinggi (74,2%), berpendidikan SD (47,5%) dan bekerja sebagai petani (41,4%). Karakteristik lokasi geografi tempat tinggal responden berhubungan dengan pengetahuan responden mengenai GAKI serta dampak dan faktor risiko GAKI (p=0,023 dan p<0,001), sedangkan pekerjaan responden berhubungan dengan pengetahuan mengenai dampak dan faktor risiko GAKI (p=0,020). Tidak ada hubungan antara pengetahuan dengan pemenuhan syarat mutu kandungan KIO3 pada garam yang digunakan di rumah tangga. Namun ada kecenderungan responden yang mempunyai garam dengan KIO3 sesuai, lebih banyak pada responden dengan pengetahuan yang baik. Kesimpulan. Responden dengan pengetahuan baik lebih banyak yang memiliki garam dengan kadar iodium sesuai standar. Perlu adanya program edukasi mengenai GAKI, penggunaan dan penyimpanan garam beriodium, serta faktor penyebab penurunan kualitas garam di rumah tangga.
Pengaruh Suhu dan Jumlah Penyeduhan terhadap Kadar Kafein Terlarut dalam Produk Teh Hijau Kering dengan Metode KCKT Febri Annuryanti; Masruratos Zahroh; Djoko Agus Purwanto
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 5 No. 1 (2018): Jurnal Farmasi dan Ilmu Kefarmasian Indonesia
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (239.328 KB) | DOI: 10.20473/jfiki.v5i12018.30-35

Abstract

Pendahuluan: Teh adalah salah satu minuman yang paling banyak dikonsumsi di dunia dan memiliki khasiat bagi kesehatan. Selain memiliki manfaat, teh juga mengandung kafein yang dapat memberikan efek samping apabila dikonsumsi secara berlebihan. Efek samping yang ditimbulkan diantaranya insomnia, ansietas, takikardia dan napas yang cepat. Oleh karena itu, saat ini diinginkan teh dengan kandungan kafein yang rendah. Tujuan: Penelitian ini bertujuan untuk menentukan efek suhu dan jumlah penyeduhan terhadap konsentrasi kafein yang terlarut dari sampel kering teh hijau. Metode: Pada studi ini digunakan tiga macam suhu (50°C, 70°C, 100°C) dan tiga kali pengulangan penyeduhan. Kafein terlarut dianalisa dengan menggunakan kondisi KCKT sebagai berikut: kolom C-18-μbondapak, fase gerak metanol : air : asam asetat 2% v/v (30:65:5) dengan laju alir 0,45 mL/menit. Deteksi dilakukan pada panjang gelombang 272 nm. Parameter validasi yang dilakukan meliputi selektifitas, linieritas, akurasi, presisi, LOD dan LOQ. Seluruh parameter validasi memenuhi persyaratan yang telah ditetapkan. Hasil: Kadar kafein tertinggi diperoleh pada penyeduhan pertama pada suhu 100oC dan kadar kafein terendah ditemukan pada penyeduhan ketiga pada suhu yang sama. Kesimpulan: Berdasarkan hasil analisis, dapat disimpulkan bahwa suhu dan jumlah penyeduhan dapat mempengaruhi kadar kafein terlarut pada teh hijau.
Co-Authors A Fahmy Arif Tsani A. Toto Purnomo Abby Rahmat Kamaruzzaman Achmad Fuad Hafid Achmad Toto Poernomo Achmad Toto Purnomo Adecha Ekalipta Primazafira Adi Utarini Afra, Fairuz Yaumil Agustina, Indri Alief Putriana Rahman Anggita Mirzautika Anita Natalia Suryawijaya Asri Darmawati Asri Darmawati Aty Widyawaruyanti Audrey Gracelia Riwu Augustien, Nora Budi Suharjo Budi Wikeko Christina Destri Corry, Maylisa Natalia Devi Eka Juniarti Dewi Isadiartuti Dewi Melani Hariyadi Edhi, Narendrani Sasmitaning Erwin Astha Triyono Esti Hendradi Fairuz Yaumil Afra Fauziyah, Riska Salfa Febri Annuryanti Guniarti Guniarti Guniarti Hadi Poerwono Hadi Suhardjono Hanifah Ridha Rabbani Hasan, Muhammad Amir Heny K Daryanto Herin Mawarti Hidayati, Agriana Rosmalina I Nyoman Wijaya Ilmi, Hilkatul Innisa, Deana Rohali Ira Widjiastuti Isnaeni Isnaeni Isnaeni Iswajuni, Iswajuni Joewono Soeroso Juni Ekowati Jusak Nugraha Jusak Nugraha Kun Ismiyatin Lahardo, Devanus M. Rasjad Indra Mangestuti Agil Marcellino Rudyanto Masruratos Zahroh Melanny Ika Sulistyowaty Mirzautika, Anggita Muhammad Amin Muslihatin, Wirda Noorma Rosita Nora Augustien Pribadi, Didik Huswo Purwaningsih - Purwitasari, Neny Retno Pudji Rahayu Retno Widyowati Riesta Primaharinastiti Riesta Primaharinastiti, Riesta Rufaidah Azzahrah Saribatiara, Saribatiara Setyabudi Setyabudi Soraya Aulia Sri Iswati Suciati Suciati Sukardiman Susetyowati Susilowati Susilowati Sutini Tiara Sukmawati Toto Sudargo Tri Widiandani Tristiana Erawati Tutiek Purwanti Ujang Sumarwan Wibowo, Nadya Khadijah Wicaksana, Firman Widiwurjani Widji Soeratri Widji Soeratri Yayuk Hartriyanti Yudianingtyas, Deanur Yusniasari, Putri Antika Yusuf, Helmy