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All Journal Jurnal Manajemen Teknologi Media Gizi Mikro Indonesia Jurnal Kedokteran Indonesia Jurnal NERS JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Science and Technology Indonesia International Humanities and Applied Science Journal Indonesian Journal of Chemistry SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat JFM (Jurnal Farmasi Malahayati) Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Jurnal Layanan Masyarakat (Journal of Public Service) Conservative Dentistry Journal Nusantara Science and Technology Proceedings Indian Journal of Forensic Medicine & Toxicology International Journal of Social and Management Studies (IJOSMAS) Berkala Ilmiah Kimia Farmasi Berkala Penelitian Hayati Camellia International Journal of Indonesian Business Review Jurnal Kefarmasian Indonesia Jurnal Sains dan Kesehatan

Djoko Agus Purwanto

Departemen Ilmu Kefarmasian, Fakultas Farmasi Universitas Airlangga, Indonesia

Author-ID : 349968

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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The Effect of Vitamin C Addition on Epigallocatechin Gallate (EGCG) Stability in Green Tea Solution Alief Putriana Rahman; Djoko Agus Purwanto; Isnaeni Isnaeni
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 6 No. 2 (2019): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v6i22019.62-68

Background: Epigallocatechin gallate (EGCG) is the most abundant green tea catechin with a powerful antioxidant effect to prevent cancer cells. EGCG in green tea solution is highly susceptible to degradation, this it is urgent to increase the stability of EGCG by vitamin C addition. Vitamin C can regenerate radical EGCG to be normal EGCG. Objective: This research aim was to determine percent of decreased level of EGCG before and after vitamin C addition. Methods: This paper was focused on enhancing the stability of EGCG by 1 mg (GTVC1), 1.5 mg (GTVC2), 2 mg (GTVC3), 2.5 mg (GTVC4) and 3 mg (GTVC5) of vitamin C addition to 10 g/L of green tea solution concentration. Evaluations of EGCG were conducted at 0 days, 1 day, 2 days, 3 days, and 4 days of storage time. EGCG was analyzed using thin layer chromatography (TLC) densitometry methods. The stability of EGCG was determined by % of decreased EGCG. Results: Percent of loss of EGCG in GT, GTVC1, GTVC2, GTVC3, GTVC4 and GTVC5 after 4 days storage were 19.93%, 10.89%, 21.08%, 18.18%, 28.56%, and 9.76%, respectively. Conclusion: This study showed that in 4 days storage, the decreased level of EGCG in green tea solution with 3 mg of vitamin C addition (GTVC5) was 9.76% which was smaller than green tea solution without vitamin C addition (GT) which EGCG decreasing 19.93%.

Effect of Guava Powder Addition on Epigallocatechin Gallate (EGCG) Content of Green Tea and Its Antioxidant Activity Hanifah Ridha Rabbani; Djoko Agus Purwanto; Isnaeni Isnaeni
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 6 No. 2 (2019): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v6i22019.85-90

Background: Tea (Camelia sinensis) contains polyphenols including epigallocatechin gallate (EGCG) which is acknowledged to have strong antioxidant properties. However, its stability is strongly influenced by environment. In a neutral and alkaline environment, EGCG could undergo degradation and lose its antioxidant property. There are some researches about the effect of combination of green tea and other plants to their antioxidant capacity. Objective: The research aimed to investigate the effect of guava addition to EGCG content of green tea and their antioxidant activity. Methods: The concentration of EGCG was determined by chromatographic analysis using TLC scanner, meanwhile the antioxidant activity was evaluated by its ability in scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) using UV-Vis Spectrophotometer. Results: Among all the samples, formula 4 (2 parts of green tea and 3 parts of guava) gave the highest EGCG content (39.03 ± 3.65 mg/g). This was 37.5% higher than the control sample (28.39 ± 2.45 mg/g). Formula 4 also had the best antioxidant activity with IC50 of 1917.32±1.75 ppm, 19% lower than control sample (2356.46 ± 3.16 ppm). Conclusion: The addition of guava powder significantly increased the amount of EGCG in green tea extracts and their antioxidant activity.

THE IMPACT OF GREEN TEA ON BLOOD FLUIDITY IMPROVEMENT AND WEIGHT LOSS Djoko Agus Purwanto; Asri Darmawati; purwaningsih purwaningsih
Jurnal Ners Vol. 9 No. 1 (2014): April 2014
Publisher : Universitas Airlangga

Introduction: Various studies have shown the benefi cial effects of green tea, not only on cardiovascular diseases but also on type 2 diabetes.Methods: In this study, the preparation of green tea water extract has been standardized to (-)-epigalocatechin gallate (EGCG), the major component of green tea. The role of green tea water extract on blood fl uidity and diabetes diseases has been studied in 13 Fructose-Fed Rat (FFR). The rats were given high fructose diet ad libitum for one week and then combination with green tea water extract every day for 6 days.Results: The results show, green tea water extract can reduces 100 μL blood passage times of wistar rat signifi cantly (p<0.01) by Micro-Channel Array Flow Analyzer (MC-FAN) instrument. Green tea water extract also had strong effect in reducing abdominal fat (p<0.05), blood glucose level (p<0.01) and body weight (p<0.01).Conclusion: These results suggest that green tea water extract may has benefi cial effects for the treatment of diabetes and reduce blood viscosity.

Skin Penetration of Topical Epigallocatechin-3-Gallate (EGCG) as an Alternative Agent for Photoaging Prevention Damayanti1,2, Cita Rosita Sigit Prakoeswa2 , Djoko Agus Purwanto3 , Anang Endaryanto4, Widjiati Widj
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11605

Photoaging is skin aging, that especially caused by chronic exposure of ultraviolet radiation. Photoagingimpacts to patients’ quality of life. Many substances, such as green tea, had been tried to be alternativeagents for photoaging prevention. Epigallocatechin-3-gallate (EGCG) is the most abundant component ingreen tea. The requirement of topical substance to be able to work and give benefit to the skin is the skinpenetration. The aim of this study was to evaluate the skin penetration of EGCG. The back part of the maleWistar mouse was shaved carefully, then topical EGCG cream 5% and 10% were administered to mouseskin. After 1 week administration, the animal was terminated and the skin biopsy was done. The mouse skinwas extracted and high performance liquid chromatography (HPLC) examination was performed to evaluatethe skin penetration of topical EGCG. The result of HPLC examination of 2 ppm, 4 ppm, 6 ppm, and 8 ppmEGCG level, showed the curve peak at 3.295, 3.296, 3.295 and 3.293 second. The basic curve showed R2 ofpure EGCG powder used in this study was 0,9999. The result of HPLC examination in this study showed,the curve peak of extracted mouse skin after EGCG cream 5% and 10% administration were between 3.2-3.7second. The result of HPLC examination in this study showed that EGCG cream could penetrate into Wistarmouse skin after EGCG cream application for 1 week.

PENGEMBANGAN INDUSTRI KREATIF SIRUP JAMU SEHAT UNTUK PEMBERDAYAAN POSDAYA DI KABUPATEN SIDOARJO Djoko Agus Purwanto; Achmad Toto Poernomo; Febri Annuryanti
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 1 No. 1 (2017): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

Indonesian people should be proud of its great natural biodiversity with precious ancestral heritages, such as Indonesian traditional medicine or Jamu. It has been intended to diversify the product of jamu that are affordable and healthy. Recently, it has been known that some luxury hotels in Indonesia creates jamu as a welcome drink, which is not only fresh but also healthful, to attract foreign customers. Empowering the community to be able making jamu syrups is importantly required for attracting many tourists to visit Indonesia. In this program, about 10 and 11 residents in the neighbourhood (RT) of 25 and 59, respectively, in Kebon Agung village, Sukodono district, Sidoarjo Regency were involved. They were trained for making jamu syrup made from lemongrass and mangosteen’s peel using health manufacturing process of traditional medicine i.e., without any chemical preserving, coloring, and taste masking agents, as well as hygienic packaging methods that can be long lasting, aesthetic appearing, and worth selling. The program consisted of training, mentoring, and lecturing to explain the making process of lemongrass and mangosteen peel syrups. The program was conducted until all participants were able to produce their own products well. The first production target was about each 200 bottles of lemongrass and mangosteen peel syrup could be sold. Bottle packaging, packaging equipment, and other supporting equipment will be delivered entirely as initial capital of POSDAYA. Hopefully, after the program is completed, the sustainability of this effort can be continued under the guidance of Universitas Airlangga. AbstrakBangsa Indonesia patut berbangga memiliki keanekaragaman hayati yang luar biasa ditambah dengan warisan nenek moyang yang berharga, salah satunya adalah jamu Saat ini, dirasakan perlu dilakukan diversiﬁkasi pengembangan jamu sebagai minuman sehat sehingga tercipta jamu yang murah, terjangkau masyarakat, dan sehat. Ada kecenderungan beberapa hotel berbintang di Indonesia menciptakan jamu sebagai welcome drink untuk menarik pelanggan, yang tidak hanya segar tetapi juga menyehatkan. Pemberdayaan masyarakat untuk bisa membuat sirup minuman jamu yang sehat perlu lebih dikembangkan sehingga diharapkan dapat menarik wisatawan yang ingin berkunjung ke Indonesia. Dalam kegiatan ini telah dapat dilatih 10 orang warga RT 25 dan 11 orang warga RT 59 Desa Kebon Agung, Kecamatan Sukodono, Kabupaten Sidoarjo dalam pembuatan sirup sereh dan sirup kulit buah manggis dengan cara yang sehat, yaitu tanpa pengawet, tanpa pewarna, dan tanpa penambah rasa, sekaligus cara pengemasannya yang higienis sehingga dapat bertahan lama, memenuhi persyaratan estetis, dan layak jual. Metode yang digunakan adalah pelatihan, pendampingan, serta ceramah untuk menjelaskan pembuatan sirup sereh dan sirup kulit buah manggis hingga peserta mampu memproduksi sendiri dengan baik dan benar. Produksi pertama ditargetkan dapat dibuat 200 botol sirup sereh dan 200 botol sirup kulit buah manggis yang layak untuk dijual. Pembelian botol, peralatan pengemasan botol, dan alat-alat pendukung lainnya akan diserahkan seluruhnya sebagai modal awal dari POSDAYA. Harapannya setelah program selesai, keberlanjutan usaha ini dapat terus dilakukan di bawah binaan Universitas Airlangga.

PELATIHAN BUDIDAYA JAMUR TIRAM SEBAGAI PROGRAM PEMBERDAYAAN SANTRI DI PESANTREN ANNUR FATMAH, DESA BELIK, KABUPATEN MOJOKERTO Achmad Toto Purnomo; Djoko Agus Purwanto
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 1 No. 1 (2017): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

A community empowerment program through training program on cultivation of Pleorotus spp mushrooms is designed as an effort to give the equality, partnership, and togetherness for improving the economic condition of social community. Islamic boarding school Annur Fatmah has potential human and natural resources, thus empowering the students of this school could provide many benefits, especially for good social changes. The program was carried out in the Annur Fatmah Islamic Boarding School in Belik village, Trawas, Mojokerto accompanied with the teams of Institute for Research and Community Service (LPPM) of Universitas Airlangga. The activities involved theory and practice for mushroom cultivation. The participants successfully cultivated mushroom for 2,500 bag-log scale production. This community empowerment activity could facilitate economic improvement by providing home income for students of Annur Fatmah Islamic Boarding School in Belik village, Mojokerto. AbstrakProgram pemberdayaan masyarakat melalui usaha budidaya jamur tiram ini dirancang sebagai upaya untuk memberikan nuansa kesetaraan, kemitraan, dan kebersamaan untuk perbaikan ekonomi masyarakat. Pondok pesantren Annur Fatmah, dengan potensi sumber daya manusia dan alamnya yang melimpah, dapat menjadi bagian dalam proses perubahan sosial. Pemberdayaan santri di pesantren ini ditujukan supaya santri dapat melakukan sesuatu yang bermanfaat bagi masyarakat, antara lain melalui program pelatihan dan pendampingan dalam usaha budidaya jamur tiram (Pleorotus spp). Dengan didampingi oleh Tim Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Airlangga, kegiatan ini dilaksanakan kepada para santri pondok pesantren Annur Fatmah di desa Belik, Trawas, Mojokerto melalui pemberian teori dan praktek lapangan tentang budidaya jamur. Kegiatan pengabdian kepada masyarakat ini telah berhasil membentuk industri kecil yang mampu memberikan pendapatan bagi santri di pondok pesantren Annur Fatmah di Desa Belik, Mojokerto.

PENGEMBANGAN PRODUK JAHE MERAH INSTANT DENGAN TECHNOLOGY SPRAY DRYING DI KAB. BANYUWANGI UNTUK KUALITAS EKSPOR Sri Iswati; Djoko Agus Purwanto; Iswajuni Iswajuni
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 1 No. 2 (2017): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

Ginger is well known in Indonesian society as one of the spices. Almost all regions in the country use ginger as an important ingredient. Along with the knowledge of the community, various packaging of ginger has been circulating, both in the form of extract and liquid. In order to increase packaging, LPPM Airlangga University will cooperate with PT ASIMAS in Lawang. So that the instant red ginger extracted through the spray drying process in Banyuwangi is packed well. The implementation method used is with training and business assistance. The results achieved from the implementation of community service in the form of training and business assistance Red Ginger Syrup Al Maghfiroh Banyuwangi. Pengmas provides information on business management and financial bookkeeping that is very important role for the progress of the company. In addition, provide preliminary information about the management of red ginger into instant products. Based on the analysis found the problem on plastic bottle packaging that has been done AbstrakJahe dikenal baik di masyarakat Indonesia sebagai salah satu rempah. Hampir semua wilayah di tanah air memanfaatkan jahe sebagai bahan masakan penting. Seiring pengetahuan masyarakat, berbagai kemasan jahe telah beredar, baik berbentuk ekstrak maupun cair. Guna peningkatan kemasan, LPPM Universitas Airlangga akan bekerjasama dengan PT ASIMAS di Lawang. Sehingga jahe merah instant yang diekstrak melalui proses spray drying di Kabupaten Banyuwangi dikemas dengan baik. Metode pelaksanaan yang digunakan adalah dengan pelatihan dan pendampingan usaha. Hasil yang dicapai dari pelaksanaan pengabdian masyarakat yakni berupa pelatihan dan pendampingan usaha Sirup Jahe Merah Al Maghﬁroh Banyuwangi. Pengmas memberikan informasi mengenai manajemen usaha dan keuangan hasil pembukuan yang sangat penting peranannya bagi kemajuan perusahaan. Selain itu, memberikan informasi awal tentang pengelolaan jahe merah menjadi produk serbuk (instans). Berdasarkan analisa ditemukan permasalahan pada kemasan botol plastik yang selama ini dilakukan.

Qualitative Analysis of Rhodamine B in Shrimp Paste at Sumbawa Besar City Markets Using Thin Layer Chromatography Abby Rahmat Kamaruzzaman; Asri Darmawati; Djoko Agus Purwanto
Berkala Ilmiah Kimia Farmasi Vol. 8 No. 2 (2021): December
Publisher : Universitas Airlangga

Terasi biasa diberi pewarna merah agar lebih menarik. Tahun 2013, pernah ditemukan Rhodamin B dalam produk terasi yang beredar di pasar kabupaten Sumbawa Barat. Rhodamin B adalah pewarna merah yang dilarang digunakan untuk makanan. Diantara berbagai produk terasi yang dijual di Sumbawa, terasi khas asli Sumbawa yaitu terasi Empang, perlu dijaga keamanannya agar dapat lebih dipromosikan. Tujuan penelitian ini adalah membuktikan bahwa terasi yang dijual pasar kota Sumbawa Besar tidak mengandung Rhodamin B. Metode yang digunakan untuk identifikasi Rhodamin B adalah kromatografi lapis tipis (KLT). Penelitian ini menggunakan silika gel 60 F254 sebagai fase diam dan fase gerak terpilih yaitu N-butanol:etil asetat:amonia 25% (10:4:5). Sampel adalah produk terasi yang dijual di Pasar Kota Sumbawa Besar, periode sampling bulan Februari dan Maret 2021. Preparasi sampel terasi dengan cara diekstraksi menggunakan etanol dan volume ekstrak yang ditotolkan pada lempeng KLT adalah 2 μl. Parameter validasi metode yang diuji adalah spesifisitas/selektifitas dan batas deteksi (LOD). Hasil penelitian menunjukkan metode ini spesifik untuk Rhodamine B yaitu Rf 0,69, profil spektra yang sama antara baku dan sampel yang diadisi Rhodamin B. Resolusi (Rs) antara noda Rhodamin B dengan noda terdekat lain dalam sampel adalah >1,5. Panjang gelombang serapan maksimum Rhodamin B adalah 544 nm. Nilai LOD sebesar 4,14 ng. Hasil identifikasi terhadap 10 sampel yang terdiri dari 3 sampel terdaftar di BPOM dan 7 sampel tidak terdaftar di BPOM tidak terdeteksi adanya Rhodamin B. Kesimpulan dari penelitian ini adalah semua sampel terasi yang dijual di pasar kota Sumbawa Besar tidak terdeteksi mengandung Rhodamin B.

In vitro culture technique of Camellia sinensis L for epicatechin production with phosphor inducer Sutini Sutini; Widiwurjani Widiwurjani; Nora Augustien; Hadi Suhardjono; Guniarti Guniarti; Djoko Agus Purwanto; Wirdhatul Muslihatin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

In several ASEAN countries, in vitro culture techniques are one of the cultivation technologies that applied in sustainable agriculture. This technology has been performed in response to obtain the secondary metabolite of epicatechin from Camellia sinensis L. However, The yield of epicatechin is remaining low than in the field production. To overcome this problem, phosphor inducers is desirable to be supplemented in the study. The young leaf shoots of C. sinensis L was sliced on a culture-filled bottle, the sliced part was re-cultivated in the same medium by supplementing of 2,4-D growth regulators, then the medium was optimized by phosphor inducer with range of concentartions 40-200 ppm. The callus formation was then evaluated using high performance liquid chromatography methods to obtain qualitative and quantitative data. The results showed that the yield of epicatechin was obtained to 11.75%, suggesting that the supplementing of phosphor inducers at cultivation of in vitro culture of C. sinensis L able to improve the production of epicatechin by in vitro culture technique.

Growth and accumulation of flavan-3-ol in Camellia sinensis through callus culture and suspension culture method Sutini Sutini; Susilowati Susilowati; M. Rasjad Indra; Djoko Agus Purwanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 1 (2016): December 2016
Publisher : The East Java Biological Society

This study was aimed to assess flavan-3-ol biomass in C. sinensis through callus cultures and suspension cultures derived from leaf explants. Callus initiation of both cultures were using Murashige and Skoog medium were enriched with plant growth regulators Naphthalene Acetic Acid 3.0 mg/L and kinetin 2.0 mg/L. The procedures in this study were: (1) callus initiation by cutting the leaves of C. sinensis shoots then planted on Murashige and Skoog medium that were enriched with plant growth regulators, (2) sub callus culture on fresh medium that enriched with the same growth regu-lators, (3) suspension culture initiation of liquid callus, (4) growth examination of callus and suspension cultures in week 12, (5) examination of qualitative-quantitative content of flavan-3-olin suspension cultures at week 4. The results show that suspension cultures contain biomass flavan-3-ol that increase in the same manner of the increase of callus age and weight.

Co-Authors A Fahmy Arif Tsani A. Toto Purnomo Abby Rahmat Kamaruzzaman Achmad Fuad Hafid Achmad Toto Poernomo Achmad Toto Purnomo Adecha Ekalipta Primazafira Adi Utarini Afra, Fairuz Yaumil Agustina, Indri Alief Putriana Rahman Anggita Mirzautika Anita Natalia Suryawijaya Asri Darmawati Asri Darmawati Aty Widyawaruyanti Audrey Gracelia Riwu Augustien, Nora Budi Suharjo Budi Wikeko Christina Destri Corry, Maylisa Natalia Devi Eka Juniarti Dewi Isadiartuti Dewi Melani Hariyadi Edhi, Narendrani Sasmitaning Erwin Astha Triyono Esti Hendradi Fairuz Yaumil Afra Fauziyah, Riska Salfa Febri Annuryanti Guniarti Guniarti Guniarti Hadi Poerwono Hadi Suhardjono Hanifah Ridha Rabbani Hasan, Muhammad Amir Heny K Daryanto Herin Mawarti Hidayati, Agriana Rosmalina I Nyoman Wijaya Ilmi, Hilkatul Innisa, Deana Rohali Ira Widjiastuti Isnaeni Isnaeni Isnaeni Iswajuni, Iswajuni Joewono Soeroso Juni Ekowati Jusak Nugraha Jusak Nugraha Kun Ismiyatin Lahardo, Devanus M. Rasjad Indra Mangestuti Agil Marcellino Rudyanto Masruratos Zahroh Melanny Ika Sulistyowaty Mirzautika, Anggita Muhammad Amin Muslihatin, Wirda Noorma Rosita Nora Augustien Pribadi, Didik Huswo Purwaningsih - Purwitasari, Neny Retno Pudji Rahayu Retno Widyowati Riesta Primaharinastiti Riesta Primaharinastiti, Riesta Rufaidah Azzahrah Saribatiara, Saribatiara Setyabudi Setyabudi Soraya Aulia Sri Iswati Suciati Suciati Sukardiman Susetyowati Susilowati Susilowati Sutini Tiara Sukmawati Toto Sudargo Tri Widiandani Tristiana Erawati Tutiek Purwanti Ujang Sumarwan Wibowo, Nadya Khadijah Wicaksana, Firman Widiwurjani Widji Soeratri Widji Soeratri Yayuk Hartriyanti Yudianingtyas, Deanur Yusniasari, Putri Antika Yusuf, Helmy

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