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Application of Rapid Multiplication Technique Using Mini Cutting and NPK Compound Fertilizer to Increase Production of Sweet Potato Cuttings (Ipomoea batatas L.) Dewi, Ni Kadek Ema Sustia; Suhartanto, M. Rahmad; Suwarto, Suwarto
Journal of Tropical Crop Science Vol. 7 No. 03 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.7.03.162-170

Abstract

Rapid Multiplication Technique (RMT) is a technique used to produce large scale cuttings of sweet potato in a short period by using mini cuttings and proper fertilizer management. The research was carried out from October 2019 to March 2020 and composed of two experiments The first experiment involved a randomized complete block design, with clones (“Ase Kapas” and “Ase Merah”) as the first factor, and tuber weight (150 ± 25 g, 250 ± 25 g, and 350 ± 25 g) as the second factor. The second experiment was arranged in a split-plot design involving different doses of NPK compound fertilizer (as main plot) and different source of cuttings (as subplots). The different doses included NPK 16:16:16 at 100 kg.ha-1 (0.3 g per polybag), 200 kg.ha-1 (0.6 g per polybag), 300 kg.ha-1 (0.9 g per polybag), and without fertilizer as control. The source of cuttings were from the tip, middle and bottom stem of the plants. Results showed that the “Ase Kapas” showed the highest number and the longest shoots, number of nodes, and length of vines. Additionally, tuber weight of ± 350 g produced the highest number of shoots. When it comes to NPK compound fertilizer treatment, a dose of 0.9 g per polybag increased cutting production in “Ase Kapas”, and also responded better to fertilizer treatment. On the other hand, dose of 0.3 g increased cutting production in “Ase Merah”. “Ase Kapas” produced more cuttings from the middle stem, whereas “Ase Merah” produced more cuttings from the . The application of RMT in “Ase Kapas” produced cuttings with ratio of 1:31, which is higher than those in “Ase Merah” with a ratio of 1:17.
Harvesting Criteria and Drying Methods to Improve the Quality of Foxtail Millet Seeds (Setaria italica L. Beauv.) Lainufar, Putri Aulia; Qadir, Abdul; Suhartanto, M. Rahmad; Ardie, Sintho Wahyuning
Journal of Tropical Crop Science Vol. 8 No. 03 (2021): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.8.03.168-176

Abstract

Foxtail millet is annual grasses with grains that are smaller than those of sorghum, rice, and wheat, and is considered one of the minor economic crops but with nutritional values similar to other major food crops. The development of millet as major cereal crop is considered challenging due to the low quality of its seeds, and studies have been conducted to improve millet’s seed quality. We conducted this study to determine the harvesting criteria of foxtail millet seeds based on the change in color of the panicles. We also wanted to determine the drying treatment, and evaluate the relationship between the position of the seed on the panicles and the seed viability and vigor. The first experiment was arranged based on a completely randomized design with panicle color as the first factor (green, yellow 75%, and brown) and drying time as the second factor (0 hours, 24 hours, 48 hours, 72 hours). The second experiment was arranged in a completely randomized design with seed position as the main factor (base, middle, tip). The study was conducted on two genotypes of millet, i.e. “BOTOK 4” and “BOTOK 10”. The highest seed quality of “BOTOK 4” and “BOTOK 10” genotypes were obtained when the panicles were brown and dried for 72 hours; the seed chlorophyll content was the lowest and water content was 8.88%, with the highest viability and vigor, i.e., germination rate of 88.3%-90%, maximum growth potential of 92%-95.3%, normal sprout dry weight 596-620 mg, vigor index of 33.6% - 21.6%, and growth rate of 18.2%-17.1% etmal-1. The highest seed viability and vigor in “BOTOK 4” genotypes were obtained from the base position, i.e., 78.7% germination rate, maximum growth potential of 83.7%, vigor index of 56.5%, growth speed of 19.6 etmal-1, normal sprout dry weight of 48 mg, radicle length of 3.3 cm) and “BOTOK 10” genotypes from the middle position with 91.5% germination, maximum growth potential of 97.2%, vigor index of 21.7%, growth speed of 17.0% etmal-1, and normal sprout dry weight 61 mg.
Development of Rapid Vigor Test Using Urine Sugar Analysis Paper for Soybean (Glycine max L.) Seeds Rukundo, Jean D’amour; Suhartanto, M. Rahmad; Ilyas, Satriyas
Journal of Tropical Crop Science Vol. 9 No. 01 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.01.22-30

Abstract

Seed vigor is an essential parameter of seed quality. It plays a critical value in the decision-making for the seeds to be used in agronomic and horticultural crops. The urinary sugar analysis paper (USAP) test determines seed vigor based on leakage concentration from the seed and corresponding color change of the USAP, which are obtained after soaking the seeds in water. This research was conducted from January 2021 to November 2021, intending to develop a rapid soybean vigor test using USAP, and it was composed of two experiments. Soybean seeds of the Biosoy1 variety were obtained from ICABIOGRAD (Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development). The seeds consisted of three different levels of seed lots (low, medium, and high viability with 35%, 64%, and 94% germination percentages, respectively). The first experiment was to determine moisture content and soaking period for the USAP seed vigor test. It was arranged in a completely randomized design with one factor as the combination of three seed lots, two levels of seed moisture content (10-12% and 13-14%), and four levels of the soaking period (0, 6, 8, and 10 hours). The second experiment was the optimization of the soaking period by seed number. It was arranged in a completely randomized design with one factor, which was combinations of three seed lots, three levels of seed numbers (50, 75, and 100 seeds), and the soaking period (four levels as 0, 6, 8, and 10 hours). The data were analyzed statistically by the Minitab package. The result showed that both combinations of 10-12% and 13-14% moisture content with the soaking period of 10 hours effectively differentiated the three seed lots into three vigor levels using USAP. Experiment 2 optimized the soaking period from 10 hours to 8 hours by using 100 seeds. The USAP color change was effectively significant to indicate seed lot vigor levels, mainly based on protein leakage concentration on USAP. The color changes from pale yellow-green for higher vigor seed lot to light greenish-blue for low vigor. The USAP was effective for the soybean vigor test for the seed lot with 10-14% moisture content by soaking 100 seeds into 50 ml of distilled water for 8 hours. The USAP is a promising rapid vigor test method but still needs to be developed further.
Ultrafine Bubbles Water priming to improve viability and vigor of bean (Phaseolus vulgaris) seeds Nurfiana, Yuni; Suhartanto, M. Rahmad; Palupi, Endah Retno; Purwanto, Y. Aris
Journal of Tropical Crop Science Vol. 10 No. 01 (2023): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.10.1.8-16

Abstract

Priming is a physiological technique of hydration of seed to improve metabolic processes before germination to accelerate germination and seedling growth under normal and stress conditions. This research is aimed to study the pretreatment using ultra-fine bubble (UFB) water to increase viability and vigor of seeds before planting (pre-planting) and during storage (pre-storage). This research was conducted at the Laboratory of Seed Quality Testing, IPB University, Indonesia. The research consisted of two experiments, i.e., UFB water priming to increase viability and vigor of bean seeds and its storability after priming. The first experiment was arranged using a completely randomized design with a combination of seed lot treatments (L1: initial germination percentage (GP) about 80%, L2: initial GP around 70%, and L3: initial GP around 60%) with priming (P0: no priming/control, P1: soaked in distilled water for 60 minutes, P2: distilled water for 120 minutes, P3: 8 ppm UFB water for 60 minutes, P4: 8 ppm UFB water for 120 minutes, P5: 20 ppm UFB water for 60 minutes, P6: UFB water 20 ppm for 120 minutes). The second experiment was arranged using a nested design with the main factor being the condition of the storage room and priming as the second factor nested in the main factor. The storage room condition factors consisted of KM: room conditions (temperature 28±5˚C, RH 73±7%) and AC: air-conditioned room (18±2 ̊C, RH 61±7%). The priming factor consisted of two levels, namely P0: without priming and P1: priming with 20 ppm UFB water soaked for 120 minutes. The results showed that priming treatment with UFB water 20 ppm for 120 minutes as a pre-planting treatment can increase the viability of bean seeds, particularly for seeds with low initial viability, and primed bean seeds with an initial viability of approximately 80% were able to retain their viability for 16 weeks of storage in an air-conditioned room.
Pengaruh Sarcotesta dan Pengeringan Benih serta Perlakuan Pendahuluan terhadap Viabilitas dan Dormansi Benih Pepaya (Carica papaya L.) Sari, Maryati; Murniati, Endang; Suhartanto, M. Rahmad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 2 (2005): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (501.084 KB) | DOI: 10.24831/jai.v33i2.1517

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Improved seed longevity by seed drying and application of  phenolic compound as natural antioxidant is the topic of this research. In many cases the sensitivity of papaya seed to drying is being the limit factor because of desiccation injury or induced dormancy. There is phenolic compound on the sarcotesta surrounding papaya seed which may act either as antioxidant or as germination inhibitor. The effect of sarcotesta and seed drying on viabillity and dormancy was studied. The experiment was conducted in July - October 2004, located at Bogor Agricultural University, used papaya seed (IPB-1) which harvested from Pusat Kajian Buah Tropika (PKBT) farm in Bogor. In the last study, seed was dried in the absence and presence of sarcotesta until 11-12% and 6-7% moisture content (mc). After drying, seed viability was measured by tetrazolium test.  The hardness of seed was also measured using penetrometer.  Seed germination was tested by (1) soaking on 10% KNO3, (2) scarification on water using  electrical stirrer, (3) soaking on 10% KNO3  with scarification using electrical stirrer, (4) scarification on the  hot water (50oC) followed by soaking on 10% KNO3.   In the absence of sarcotesta, the viability of 6-7% mc seed was as high as 11-12% mc seed. There was neither viability reduction nor induced dormancy. Whereas in the presence of sarcotesta, there was also no viability reduction but the dormancy was induced. The dormancy of seed with 11-12% mc was longer than seed with 6-7% mc. Scanning electron microscopy images showed that sarcotesta was removed by cleaning treatment before drying. On the contrary, sarcotesta was not completely removed from the seed and became more impermeable when cleaning was done after drying.This research can not suggest the most effective  pre-treatment to break the dormancy. The mechanism of the dormancy is discused.   Key words: Carica papaya L., seed drying, sarcotesta, seed viability, seed dormancy
Fluoresen Klorofil Benih: Parameter Baru dalam Penentuan Mutu Benih Suhartanto, Mohamad Rahmad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 31 No. 1 (2003): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (47.252 KB) | DOI: 10.24831/jai.v31i1.1527

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It has been shown that chlorophyll content of seeds was negatively correlated with germinability towards the end of maturation.  Physiological maturity was achieved when the chlorophyll fluorescence reached a minimum.  The presence of chlorophyll in seeds and its relation with the progress of seed maturation has gained renewed interest after the development of Laser Induced Fluorescence (LIF).  This equipment is able to measure and analyze chlorophyll fluorescence in the seed instantaneously and non-destructively.  The use of LIF makes it possible to perform physiological and biochemical assays after chlorophyll fluorescence measurement in the same seeds.  Based on the results from some experiments as well as the literature, the role of chlorophyll in developing seeds is presented.  The overall conclusion is that chlorophyll is required during seed development, but undesirable during maturation.  We hypothesize that the presence of chlorophyll during seed maturation is undesirable since it is associated with lower quality, particularly lower seed longevity.  Chlorophyll may also be a primary source of free radicals.  Seed chlorophyll fluorescence was affected by endogenous abscisic acid, gibberellins and phytochrome. Light, temperature and relative humidity may also influence the chlorophyll fluorescence of seeds.   Key words: Chlorophyl, Fluorescence,  Parameter, Seed testing
Metode Pengusangan Cepat untuk Pengujian Vigor Daya Simpan Benih Cabai (Capsicum annuumL.) Ekowahyuni, Luluk Prihastuti; Sutjahjo, Surjono Hadi; Sujiprihati, Sriani; Suhartanto, Mohamad Rahmad; Syukur, Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 40 No. 2 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.268 KB) | DOI: 10.24831/jai.v40i2.6374

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The ability of seed to maintain seed quality during storage is called seed vigor. Accurate method for seed vigor testing of pepper seeds is necessary to accurately determine seed storability during seed distribution. The aim of this research was to determine the best accelerated aging method (AAM) of pepper seed. Freshly harvested seeds of Capsicum annuumL., IPB C9 genotype were used in this study. The experiment used randomized complete block design (RCBD) with three replications. The experimental treatments were seed vigor testing methods i.e. natural deterioration test at room temperature in controlled humidity (RH 90-95%), and four AAM (hot water 60 ºC, methanol 20%, ethanol 20%, and high temperature at 40 ºC). The best method was selected using analysis of variance, coeficient of variance, regresion dan t student analysis. AAM using 20% methanol at periods of 0, 2, 4, 6 and 8 hours was the best method of seed vigor testing for pepper seed. Keywords: accelerated aging, deterioration, ethanol, methanol, high temperature
Inisiasi Tunas Ganda Tanaman Manggis Malinau melalui Kultur In Vitro untuk Perbanyakan Klonal Lestari, Endang Gati; Suhartanto, M. Rahmad; Kurniawati, Ani; Rahayu, Suci
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 1 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.474 KB) | DOI: 10.24831/jai.v41i1.7075

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Mangosteen (Garcinia mangostana L.) is one of the most promising tropical fruits for export. The major constraint toincrease fruit production of the spesies is the long juvenile period. Seedless, sweet and juicy variety of mangosteen had beenfound in Malinau. In vitro propagation technique offers possibility to produce sufficient number of seedlings any time. Thisresearch was aimed at obtaining the appropriate media formula to enhance shoot proliferation. This research consisted ofshoot induction and multiplication and shoot elongation. The materials were the fresh mangosteen seeds from the Malinaumangosteen trees. The explant used in the trial was seeds which were divided into four slices. The use of 8 to 16 mg BA L-1combined with 0.2 mg thidiazuron L-1 resulted in the best shoot induction of 52 shoot buds per explant at the 6th week afterplanting with the mean height of 0.3 cm. Upon subculturing in to the similar media, the number of shoot tends to increase.For multiplication, low concentration of BA (2 to 4 mg L-1) and thidiazuron 0.05 mg L-1 were applied to increase the numbersof shoots. The total shoot number obtained in the media with 0.05 thidiazuron without BA was 11.25 and in the media with 2mg BA L-1 + 0.05 mg thidiazuron L-1 was 8.7 shoot explant-1. The result showed that the best media for shoot elongation wasMS + 1 mg BA L-1 + 2 mg kinetin L-1. The length of the shoots were in the range of 0.5-0.8 cm.Keywords: BA, Garcinia mangostana, in vitro culture, shoot multiplication, thidiazuron
Optimasi Pengeringan Benih Jagung dengan Perlakuan Prapengeringan dan Suhu Udara Pengeringan Rofiq, Muhammad; Suhartanto, Mohamad Rahmad; Suharsi, Tatiek Kartika; Qadir, Abdul
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 3 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.278 KB) | DOI: 10.24831/jai.v41i3.8096

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ABSTRAKPenelitian ini bertujuan untuk meningkatkan efisiensi pengeringan benih jagung melalui pengembangan rancangan sistempengeringan dan melakukan kombinasi perlakuan prapengeringan dengan suhu udara pengeringan untuk mendapatkan mutubenih yang maksimum. Prapengeringan dapat dilakukan dengan cara menghembuskan udara suhu kamar menggunakanmesin blower dan dilakukan sebelum benih jagung diberikan perlakuan udara panas. Penelitian terdiri atas 3 tahap, yaitu(1) Perancangan sistem pengeringan, (2) Optimasi pengeringan benih jagung, dan (3) Analisis ekonomi. Kegiatan pertamaterdiri atas 2 tahap, yaitu pembuatan dan pengujian mini box dryer. Optimasi pengeringan benih jagung terdiri atas 2 faktorperlakuan, yaitu prapengeringan (0, 12, 24, dan 36 jam), dan suhu udara pengeringan (40, 45, 50, dan 55 °C), menggunakanrancangan kelompok lengkap teracak dengan 3 ulangan. Analisis mutu fisik dan fisiologis dilakukan untuk mendapatkanperlakuan yang mampu menghasilkan benih dengan kualitas baik. Analisis ekonomi dilakukan untuk mengetahui perlakuanyang memiliki B/C Ratio paling tinggi. Hasil percobaan menunjukkan bahwa kombinasi perlakuan prapengeringan 36jam dan suhu udara pengeringan 50 °C merupakan perlakuan optimum pada pengeringan benih jagung, karena mampu menghasilkan benih dengan kualitas baik dan memiliki B/C Ratio paling tinggi.Kata kunci: mini box dryer, optimasi pengeringan benih, prapengeringan, suhu udara pengeringan
Co-Authors ABDUL MUNIF Abdul Munif Abdul Qadir Agus Purwantara Agus Purwantara AGUS PURWANTARA Agus Ruhnayat AHMAD JUNAEDI Ahmad Zamzami Akhiruddin Maddu Alfarabi, Emir Aqsha Ani Kurniawati Ari Wahyuni Arodi Agustenta Sinulingga Astryani Rosyad Aulia Hasan Widjaya Baharudin Baharudin ; BAHARUDIN BAHARUDIN Cahyani, Gesa Nur Cintaning, Anis Bias Daniel Happy Putra Dede J. Sudrajat Dede J. Sudrajat DEVI RUSMIN DEWI, NI KADEK EMA SUSTIA Diaguna, Ridwan Dian Latifah Dirgahani Putri Dyah - Manohara Edi Santosa Eka Maulidiya, Sherly Endah R. Palupi Endah Retno Palupi Endang Gati Lestari ENDANG MURNIATI Eny Widajati Eprilian, Husna Fatima Evayusvita Rustam Evayusvita Rustam Fadillah, Arvin Muhammad Fatiani Manik Fatiani Manik Firdaus, Jonni Fitri Viva Yuningsih, Aida Fitriansyah, Muhammad Ramdhani Heri Ahmadi Heru Setiawan Heru Setiawan Ikasari, Yulfa Astuti Karmaita, Yummama Kartika Kartika Kartika Kartika Ketty Suketi Khairani Khairani Lainufar, Putri Aulia Lilih Naelun Najah Luluk Prihastuti Ekowahyuni Manohara, Dyah - Maryati Sari Mubarak, Farahdina Muhamad Noor Azizu, Muhamad Noor Muhamad Syukur Muhammad Abdul Rahman Hakim Muhammad Rofiq Nabila, Diah Ayu Nafisah Nafisah Neti, Natali Nurfiana, Yuni NURUL HIDAYAH Nurul Hidayah Nurul Rostami Dewi Nurul Rostami Dewi Otih Rostiana Palupi, Endah R. Pipin Apriani Purwono Purwono, Purwono Putri Mian Hairani Putri, Lystiana Dewi Qadir, Abdul Risa Wentasari Roedhy Poerwanto Rokhani Hasbullah Rukundo, Jean D’amour RUSMIN, DEVI Satriyas Ilyas SATRIYAS ILYAS Setyowati, Nur Farida Sinaga, Tamara Rudang Astari Sintho Wahyuning Ardie SRIANI SUJIPRIHATI Suci Rahayu Supramana Surjono Hadi Sutjahjo Suryo Wiyono Suwarto Suwarto Syarifa Mustika Syarifa Mustika Taisa, Rianida Tatiek K. Suharsi Tatiek K. Suharsi Tatiek Kartika Suharsi Usman Ahmad Widodo Widodo Zulfa Fauziyyah Taini