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Development of Rapid Viability Test Using Urine Sugar Analysis Paper for Peanut (Arachis hypogaea L.) Seeds Mubarak, Farahdina; Suhartanto, Mohamad Rahmad; Widajati, Eny
Journal of Tropical Crop Science Vol. 12 No. 02 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.02.378-387

Abstract

Seed testing is important for determining seed quality. Seed testing must be easy, quick, and accurate. This study aimed to develop a rapid method for assessing peanut seed quality using Urine Sugar Analysis Paper (USAP). USAP is commonly used for detecting human urinary glucose and is currently being developed for evaluating seed quality. Three experiments were conducted. Experiment 1 investigated the most effective seed-cutting size (whole, halved, and seeds cut into six pieces) and soaking periods (0, 3, 6, and 9 hours) based on glucose and protein leakage, as well as specific gravity of the soaking water. The optimal method identified in Experiment 1 was then used to test all five seed lots in USAP Experiment 2, which aimed to evaluate the effectiveness of seed quality testing using the USAP method at various levels of seed viability. Experiment 3 investigated the correlation between USAP test results and other viability and vigor test parameters, including germination percentage, first count germination, speed of germination, and electrical conductivity. The results of experiments indicated that cutting seeds into six pieces and 6 6-hour soaking period was the most optimal. Experiment 3 showed that USAP testing correlated with viability and vigor testing across various peanut varieties. Protein and density testing using USAP were negatively correlated with germination percentage, first count germination, and germination speed parameters, but positively correlated with electrical conductivity. However, glucose leakage was not detectable using USAP.
Karakteristik fisik carbon dots dan aplikasinya dengan pemupukan untuk meningkatkan produksi dan mutu benih padi: Physical characteristics of carbon dots and its application with fertilizer to increase rice seed production and quality Fitri Viva Yuningsih, Aida; Nafisah, Nafisah; Maddu, Akhiruddin; Rahmad Suhartanto, Mohamad
Jurnal AGRO Vol. 12 No. 1 (2025)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/j.agro.46880

Abstract

Carbon dots (CDs) are nano-sized carbon particles proven to enhance seed germination, plant growth, and yield. This study evaluated CDs in an integrated fertilization system combining macronutrient fertilizers (NPK) and organic matter (OM). The experiment was conducted during the 2023 dry season at the Sukamandi Experimental Station of BBRMP Padi using a Split-split Plot Design. The treatments included NPK combinations (main plot; without NPK, +PK, +NP, +NK, +NPK), OM (sub-plots; manure, straw compost, without OM), and foliar-applied CDs (800 mg L⁻¹) (sub-sub-plots; +CDs, without CDs). The results showed that coffee ground-based CDs from coffee shops were water-soluble, light brown, and had an absorption peak at 300 nm. Nitrogen doping with urea increased the N content from 2,44% to 16,12%. The N and P significantly improved vegetative growth, grain yield, and seed quality, while N and K maintained chlorophyll in the reproductive stage. OM increased NPK efficiency and nutrient availability, with manure more effective than straw compost. CDs enhanced OM in increasing leaf area. The combination of NPK, OM, and CDs shows potential as an efficient and sustainable fertilization strategy to increase rice productivity and seed quality.   ABSTRAK Carbon dots (CDs) adalah partikel karbon berukuran nano yang telah terbukti mampu meningkatkan perkecambahan benih, pertumbuhan, dan hasil tanaman. Penelitian ini bertujuan untuk mengevaluasi CDs dalam sistem pemupukan terintegrasi dengan pupuk hara makro (NPK) dan bahan organik. Penelitian dilaksanakan pada Musim Kemarau 2023 di lahan sawah irigasi Kebun Percobaan Sukamandi BBRMP Padi  dengan Rancangan Petak-petak Terbagi. Kombinasi pupuk NPK (petak utama; tanpa NPK, +PK, +NP, +NK, +NPK), bahan organik (anak petak; pupuk kandang, kompos jerami, tanpa BO), dan aplikasi foliar CDs (800 mg L-1) (anak-anak petak; tanpa CDs, +CDs). Hasil percobaan menunjukkan CDs berbasis ampas kopi dari limbah coffee shop memiliki sifat larut air, berwarna cokelat terang, dan puncak absorbansi pada 300 nm. Pengkayaan nitrogen dengan urea meningkatkan kandungan N dari 2,44% menjadi 16,12%. Kombinasi N dan P sangat mempengaruhi pertumbuhan vegetatif, hasil gabah, dan mutu fisiologis benih. Kombinasi N dan K efektif dalam mempertahankan klorofil pada fase reproduktif. Bahan organik terbukti meningkatkan efisiensi pupuk NPK dan ketersediaan hara, dimana pupuk kandang lebih efektif dibandingkan kompos jerami. Aplikasi CDs meningkatkan efektivitas bahan organik dalam meningkatkan luas daun. Kombinasi pupuk NPK, bahan organik, dan CDs berpotensi menjadi strategi pemupukan efisien dan berkelanjutan untuk meningkatkan produktivitas dan mutu benih padi. Kata kunci: Bahan organik, hasil, luas daun, pupuk NPK, sinergi pupuk
The Penggunaan Pupuk Hayati Endomikoriza untuk Meningkatkan Efisiensi Pemupukan NPK pada Jagung Manis Suwarto, Suwarto; Rahmad Suhartanto, Mohamad
Jurnal Hortikultura Indonesia (JHI) Vol. 16 No. 2 (2025): Jurnal Hortikultura Indonesia (JHI)
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.16.2.117-124

Abstract

Nitrogen (N), phosphorus (P), and potassium (K) nutrients are essential for sweet corn growth and yield. N, P, and K fertilizer is increasingly limited and expensive, so it should be an efficient application. Endomycorrhiza is a mycorrhiza that is symbiotic with roots, forming a hyphae network that expands the root surface to absorb nutrients.  The objective of this research was to study the role of Endomycorrhiza to the efficiency of N, P, and K fertilization. There were six levels: without fertilization, fertilization of 1.00 dose of Standard NPK, 1.00 dose of Standard NPK+Endomycorrhiza, 0.75 dose of Standard NPK+Endomycorrhiza, 0.50 dose of Standard NPK+Endomycorrhiza, and 0.25 dose of Standard NPK+Endomycorrhiza. Standard NPK is 135 kg N, 72 kg P2O5, and 120 kg K2O per hectare. The dose of Endomycorrhizal biofertilizer (2.1 x 103 CFU g-1 active mycorrhizal propagules) was 800 kg per hectare. The treatments were repeated 4 times. The number of leaves at 8 weeks after planting without fertilization was the least (11.8 leaves), which were fertilized with 1.00, 0.75, 0.50, and 0.25 doses of Standard NPK+Endomycorrhizae were more (average 13.7 leaves) than 1.0 dose of Standard NPK without mycorrhizae (12.9 leaves). The yield of husked cobs from 1.00, 0.75, and 0.50 doses of Standard NPK+Endomycorrhizae were similar and higher (average 5.44 tons ha-1) than 1.00 dose of Standard NPK without Endomycorrhizae (4.72 tons ha-1). The Endomycorrhiza biofertilizer could save 50% of the standard NPK dose with 11.2% higher results.  Keywords: corn cob weight, hyphae, mycorrhiza, propagules, symbiosis
the Seed Dormancy of Melon Seeds (Cucumis melo L.): Causes, Types and the Effectiveness of Plasma Treatment in Breaking Dormancy: Seed Dormancy of melon seed and it's breaking Setyowati, Nur Farida; Suhartanto, M. Rahmad; Purwanto, Y. Aris
Jurnal Hortikultura Indonesia (JHI) Vol. 16 No. 2 (2025): Jurnal Hortikultura Indonesia (JHI)
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.16.2.70-76

Abstract

Plasma adalah gas terionisasi yang menghasilkan spesies oksigen reaktif (ROS) dan spesies nitrogen reaktif (RNS), yang dapat meningkatkan imbibisi benih. Tujuan penelitian ini adalah menentukan tipe dormansi benih melon dan memanfaatkan plasma untuk pematahan dormansi. Penelitian terdiri atas dua percobaan. Percobaan pertama menentukan penyebab dormansi benih melon dengan mengamati perubahan fisiologis dan hormon selama 10 minggu, serta mengupas testa dan tegmen yang menghambat imbibisi benih. Lot benih yang disimpan diuji perkecambahannya serta dilakukan analisis hormon ABA dan GA setiap minggu. Percobaan kedua mengaplikasikan plasma pada benih melon yang dilembabkan dan tidak dilembabkan dengan voltase (100, 150, 225) volt, serta optimasi waktu perendaman benih dalam air selama (2, 4, 6) jam dengan voltase 225 volt. Hasil penelitian menunjukkan bahwa selama periode penyimpanan 0 -10 minggu terjadi fluktuasi kandungan hormon giberelin (GA), asam absisat (ABA) dan Daya Berkecambah (DB). Pengupasan testa dan tegmen dapat meningkatkan DB menjadi 89%. Konsentrasi hormon GA pada benih yang telah diberi perlakuan plasma lebih tinggi dibandingkan dengan benih tanpa perlakuan, namun belum mampu meningkatkan DB. Optimasi waktu perendaman benih dalam air selama 2 jam sebelum pemaparan plasma mampu meningkatkan DB. Benih melon masih memiliki dormansi hingga 10 minggu dan memiliki dormansi fisik yang lebih dominan dibandingkan dormansi fisiologis. Pematahan dormansi pada perlakuan plasma 225 volt dengan perendaman benih selama maksimum 2 jam sebelum pemaparan plasma dapat mematahkan dormansi benih melon dengan DB 83.5%.
ANALISIS KOMPARATIF PERTUMBUHAN DAN HASIL TANAMAN BAWANG MERAH (Allium Cepa L. Aggregatum) BERDASARKAN UKURAN BAHAN TANAM Suhartanto, Mohamad Rahmad; Wahyuni, Ari; Cintaning, Anis Bias; Nabila, Diah Ayu; Neti, Natali; Sinaga, Tamara Rudang Astari; Wentasari, Risa; Taisa, Rianida; Karmaita, Yummama
Jurnal Agroteknologi Vol 16, No 1 (2025): Agustus 2025
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v16i1.37633

Abstract

Shallot development faces an obstacle, specifically the availability of quality seeds. Onion propagation is usually done conventionally with vegetative propagation methods using bulbs as seeds. The aims of study were to determine the effect of the size of the shallot bulb planting material on the growth and production of shallots, to obtain the variables used in selecting the size of the shallot bulbs.The research was conducted at Leuwikopo Experimental Farm in Dramaga, Bogor from February to May 2024. The experiment was conducted by comparing two treatments and each treatment was repeated 9 times. The treatment used was bulb size, especially large and small. The data obtained were tested using the t-student test at the 5% α level, which compares the results of observations between large and small bulbs. The variable observed consisted of plant height, number of shoots, number of leaves, number of bulbs, number of multiplications, wet weight, and dry weight of bulbs. The results showed that different sizes of bulbs used as shallot plant propagation materials affect the growth and production of shallots. Shallot seeds with large bulb sizes produce better growth than small bulbs. The size of large and small bulbs produces output in the form of variable of the number of bulbs per clump, and wet and dry weights of bulbs that are not different. The variables number of leaves, number of shoots, dry bulb weight, and multiplication rate can be used as characteristics for selecting bulb size.
Co-Authors ABDUL MUNIF Abdul Munif Abdul Qadir AGUS PURWANTARA Agus Purwantara Agus Purwantara Agus Ruhnayat AHMAD JUNAEDI Ahmad Zamzami Akhiruddin Maddu Alfarabi, Emir Aqsha Alvita Sekar Sarjani Ani Kurniawati Ari Wahyuni Arodi Agustenta Sinulingga Astryani Rosyad Aulia Hasan Widjaya Baharudin Baharudin ; BAHARUDIN BAHARUDIN Cahyani, Gesa Nur Cintaning, Anis Bias Dede J. Sudrajat Dede J. Sudrajat DEVI RUSMIN Diaguna, Ridwan Dian Latifah Dirgahani Putri Dyah - Manohara Edi Santosa Eka Maulidiya, Sherly Endah R. Palupi Endah Retno Palupi Endang Gati Lestari ENDANG MURNIATI Eny Widajati Evayusvita Rustam Evayusvita Rustam Fadillah, Arvin Muhammad Fatiani Manik Fatiani Manik Firdaus, Jonni Fitri Viva Yuningsih, Aida Fitriansyah, Muhammad Ramdhani Heri Ahmadi Heru Setiawan Heru Setiawan Husna Fatima Eprilian Karmaita, Yummama Kartika Kartika Kartika Kartika Ketty Suketi Khairani Khairani Lilih Naelun Najah Luluk Prihastuti Ekowahyuni Luluk Prihastuti Ekowahyuni Manohara, Dyah - Maryati Sari Mubarak, Farahdina Muhamad Noor Azizu, Muhamad Noor Muhamad Syukur Muhammad Abdul Rahman Hakim Muhammad Rofiq Nabila, Diah Ayu Nafisah Nafisah Neti, Natali NURUL HIDAYAH Nurul Hidayah Nurul Rostami Dewi Nurul Rostami Dewi Otih Rostiana Palupi, Endah R. Pipin Apriani Purwono Purwono, Purwono Putri Mian Hairani Putri, Lystiana Dewi Qadir, Abdul Risa Wentasari Roedhy Poerwanto Rokhani Hasbullah RUSMIN, DEVI Satriyas Ilyas Setyowati, Nur Farida Sinaga, Tamara Rudang Astari SRIANI SUJIPRIHATI Sriani Sujiprihati Suci Rahayu Supramana Surjono Hadi Sutjahjo Suryo Wiyono Suwarto Suwarto Syarifa Mustika Syarifa Mustika Taisa, Rianida Tatiek K. Suharsi Tatiek K. Suharsi Tatiek Kartika Suharsi Usman Ahmad Widodo Widodo Zulfa Fauziyyah Taini