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Effect of Human Bone Marrow Mesenchymal Stem Cells on Burn Healing in Granulation Tissue Formation in Diabetic Rats Gusti Revilla; Deddy Saputra; Dini Nurhasanah
Jurnal Kesehatan Andalas Vol 11, No 3 (2022): Online November 2022
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v11i3.2089

Abstract

Burns with diabetes mellitus can interfere with the wound-healing process. Mesenchymal stem cells have been studied to accelerate the healing of burns, one of which originates from the bone marrow. Objective: To determined the effect of human bone marrow mesenchymal stem cells (hBM-MSCs) on burn wound healing in granulation tissue formation in Diabetes Mellitus rats. Methods: This study was an experimental study with a post-test-only control group design, using 30 rats divided into two groups, the control group Phosphate Buffer Saline (PBS) and the treatment group (hBM-MSCs). Mice were induced by alloxan to cause hyperglycemia. Burns are made using a heated plate. Tissue collection was carried out after the termination of the experimental animals on the 3rd, 7th and 14th days and then histological preparations were made to assess the formation of granulation tissue. Data were analyzed using the two-way ANOVA test. Results: There was an increase in the number of macrophages, fibroblasts and new blood vessels after being given hBM-MSCs. On day 14 neutrophils and lymphocytes on day 3, there was no increase. Statistical analysis showed a significant increase in lymphocytes, macrophages, fibroblasts and new blood vessels. Conclusion: Human bone marrow mesenchymal stem cells can increase the formation of granulation tissue by increasing the number of macrophages, fibroblasts and new blood vessels in diabetic rats.Keywords: burn wound,  diabetic rat, human bone marrow mesenchymal stem cells, granulation tissue
Gambaran Faktor Risiko Penderita Stroke dengan COVID-19 di RSUP Dr. M. Djamil Padang Musrizal Adli; Restu Susanti; Gusti Revilla; Afriwardi Afriwardi; Dedi Sutia; Eldi Sauma
Jurnal Ilmu Kesehatan Indonesia Vol 4 No 3 (2023): September 2023
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jikesi.v4i3.1054

Abstract

Latar Belakang : Stroke merupakan penyakit dengan angka kematian tertinggi kedua di dunia. Pada massa pandemi COVID-19 berbagai temuan neurologis termasuk stroke ditemukan pada penderita COVID-19. Terjadinya stroke pada pasien COVID-19 dipicu keadaan hiperinflamasi dan hiperkoagulasi yang umum terjadi pada pasien COVID-19. Objektif : Mengetahui gambaran faktor risiko penderita stroke dengan COVID-19 di RSUP Dr. M. Djamil Padang periode Maret 2020-Desember 2021. Metode : Penelitian ini menggunakan metode cross sectional menggunakan data rekam medis pasien yang terdiagnosis stroke dengan COVID-19. Pengambilan sampel dengan metode total sampling. Analisis data secara univarian untuk mencari distribusi frekuensi dan bivariat menggunakan uji fisher exact untuk mengetahui hubungan faktor risiko dengan penyakit storke pada pasien COVID-19. Hasil : Hasil penelitian didapatkan 381 pasien stroke. Pasien stroke dengan COVID- 19 sebanyak 36 orang (9,4%). Stroke iskemik dengan COVID-19 sebanyak 26 orang (72,22%) dan stroke hemoragik dengan COVID-19 sebanyak 10 orang (27,78%). Kelompok usia terbanyak ≥65 tahun (47,2%) dan lebih banyak berjenis kelamin laki-laki (55,5%). Urutan faktor risiko mulai dari yang terbanyak yaitu hiperkoagulsi, hipertensi, diabetes melitus, dislipidemia, obesitas, dan riwayat penyakit jantung. Penelitian ini menunjukkan adanya hubungan antara hipertensi dengan terjadinya stroke dengan COVID-19. Kesimpulan : Frekuensi pasien stroke dengan COVID-19 sebesar (9,4%). Pasien umumnya mengalami gangguan koagulasi, ditandai dengan peningkatan nilai D-dimer. Pada penelitian ini terdapat hubungan antara faktor risiko hipertensi dengan kejadian stroke pada pasien COVID-19. Kata kunci: Faktor risiko, Stroke, Iskemik, Hemoragik, COVID-19 Background : Stroke is the sceond leading highest mortality rate in the world. During the COVID-19 pandemic, various neurological findings including stroke in patient with COVID-19 disease. Stroke in COVID-19 patients is triggered by hiperinflammation and hypercoagulability which are common in COVID-19 patients. Objective : To describe the risk factors of stroke on COVID-19 Patients hospitalized in DR. M. Djamil Padang during Maret 2020-Desember 2021 period. Methods : This study used a cross-sectional method using medical record data of patients diagnosed with stroke in COVID-19 patients. The sample was taken using the total sampling method. The data analysis was conducted Univariate to find the frequency distribution and bivariate using the Fisher's exact test to determine the relationship between risk factors and stroke in COVID-19 patients. Results : The result of study were 381 patients strokes. There are 36 patients strokes with COVID-19. Patient ischemic stroke with COVID-19 were 26 (72,22%) and hemorrhagic stroke with COVID-19 were 10 (27,78%). The most age group is ≥65 years old (47,2%) and the gender are more male (55,5%). The sequence of risk factors starting from the most is hypercoagulation, hypertension, diabetes mellitus, dyslipedimia, obesity, and history of heart disease. This study showed relationship between hypertension and the occurence of stroke in COVID-19 patients. Conclusion : The frequency of stroke in COVID-19 patient is 9,4%. Patient generally had coagulation disorders which are showed by increased D-dimer values. This study also showed the relationship between hypertension and the occurence of stroke in COVID-19 patients while other risk factors have no relationship. Keyword: Risk Factors, Stroke, Ischemic, Hemorrhagic, COVID-19
Pengaruh Ekstrak Etanol Peronema canescens Jack terhadap Ekspresi Gen Tumor Necrosis Factor Alpha pada Sel HeLa Ansharina, Hafiznie; Revilla, Gusti; Kadri, Husnil; Desmawati, Desmawati; Yusri, Elfira; Ali, Hirowati
Health and Medical Journal Vol 7, No 1 (2025): HEME January 2025
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/heme.v7i1.1418

Abstract

Latar Belakang: Kanker serviks merupakan kanker yang menduduki peringkat keempat pada wanita di seluruh dunia. Kanker ini disebabkan oleh Human Papillomavirus (HPV) tipe resiko tinggi yang menyerang sel-sel leher rahim. Sel kanker akan mensekresi Tumor Necrosis Factor Alpha (TNF-α) untuk menjadikan lingkungan mikrotumor mendukung bagi kelangsungan hidup sel kanker. Terapi kanker serviks yang umum digunakan adalah pembedahan, radioterapi, dan kemoterapi. Terapi lain yang juga digunakan adalah tanaman herbal, contohnya sungkai. Ekstrak etanol daun sungkai (Peronema canescens J) mengandung senyawa yang berperan sebagai antiinflamasi dan antioksidan. Tujuan: Penelitian ini bertujuan untuk mengetahui pemberian ekstrak etanol daun sungkai dalam menurunkan ekspresi gen TNF-α pada sel HeLa. Metode: Penelitian ini merupakan penelitian true experimental dengan post test only control group design yang menggunakan 24 well plate kultur sel HeLa yang tumbuh secara konfluens. Sampel dibagi menjadi empat kelompok (K, P1, P2, dan P3). Kelompok perlakuan diberikan ekstrak etanol daun sungkai dengan konsentrasi inhibition concentration (IC)25, IC50, dan IC75 yang diinkubasi selama 24 jam. Sel yang telah diberi perlakuan akan dipanen untuk dilakukan isolasi RNA. Pemeriksaan gen TNF-α dilakukan menggunakan PCR Konvensional, kemudian band elektroforesis berukuran 47 bp dianalisis menggunakan ImageJ. Hasil: Data didapatkan dari analisis ketebalan band elektroforesis yang diukur menggunakan ImageJ. Analisis data dilakukan menggunakan uji One Way ANOVA dan Post Hoc Bonferroni, didapatkan rerata ekspresi gen TNF-α pada kelompok K, P1, P2, dan P3 secara berturut-turut adalah 0,32; 0,29; 0,23; dan 0,14. Terdapat perbedaan yang bermakna antara kelompok K terhadap P2 dan P3 dengan nilai p=0,000 (p<0,05), sedangkan kelompok K dengan P1 perbedaannya tidak bermakna (p>0,05). Kesimpulan: Pemberian ekstrak etanol daun sungkai pada konsentrasi IC50 dan IC75 mampu menurunkan ekspresi gen TNF-α pada kelompok sel HeLa.
Profil Pasien Lupus Eritematosus Kutaneus di Rumah Sakit Umum Pusat Dr M Djamil Padang Periode 2015 - 2020 Tiffany, Begum; Akhyar, Gardenia; Revilla, Gusti; Asri, Ennesta; Tofrizal, Tofrizal; Afriani, Nita
Seroja Husada: Jurnal Kesehatan Masyarakat Vol. 1 No. 6 (2024): Seroja Husada: Jurnal Kesehatan Masyarakat
Publisher : Seroja Husada: Jurnal Kesehatan Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lupus eritematosus kutaneus (LEK) merupakan salah satu dari jenis penyakit lupus eritematosus (LE) yang merupakan penyakit autoimun dengan variasi klinis luas. Walaupun penyebab lupus jenis manapun belum jelas diketahui, salah satu faktor pencetus yang mungkin jadi penyebab penyakit ini adalah faktor hormon, paparan sinar matahari, dan faktor lainnya. Sama seperti lupus eritematosus lainnya, LEK lebih sering terjadi pada wanita usia subur dibandingkan dengan laki laki dengan manifestasi klinis yang muncul di kulit yang dapat dipicu karena paparan sinar matahari. Hal ini ditambah dengan manifestasi klinis lain selain manifestasi pada kulit yang juga muncul, dapat menimbulkan masalah yang mempengaruhi kualitas hidup penderitanya.Tujuan dari penelitian ini adalah untuk mengetahui profil klinis pasien LEK di RSUP Dr M Djamil Padang periode 2015-2020. Populasi dalam penelitian ini adalah seluruh pasien LEK di RSUP Dr M Djamil Padang periode 2015-2020, dengan total 20 data. Data sekunder berupa rekam medis pasien LEK digunakan dalam penelitian ini, dan disajikan dalam bentuk narasi dan tabel distribusi frekuensi.Hasil penelitian ini diperoleh jumlah penderita LEK di Rumah Sakit Umum Pusat Dr M Djamil Padang tahun 2015 - 2020 adalah sebanyak 17 orang penderita LES dan LEK, dan 3 orang penderita LEK saja dari 354 orang penderita LE. Jumlah pasien LEK perempuan jauh lebih banyak dari pasien laki laki. Distribusi kelompok umur terbanyak adalah berusia 15-24 tahun.
Adipose-Derived Mesenchymal Stem Cell (AD-MSC)-Like Cells Restore Nestin Expression and Reduce Amyloid Plaques in Aluminum Chloride (AlCl3)-Driven Alzheimer's Rat Models Annita, Annita; Revilla, Gusti; Ali, Hirowati; Almurdi, Almurdi
Molecular and Cellular Biomedical Sciences Vol 8, No 3 (2024)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v8i3.387

Abstract

Background: Alzheimer's disease (AD) is a neurodegenerative disorder with a significant burden on public health, and current treatments offer limited efficacy. This study investigated the effectiveness of adipose-derived mesenchymal stem cells (AD-MSCs) on the expression of the nestin gene and amyloid plaque in an aluminum chloride (AlCl3)-driven Alzheimer's rat model.Materials and methods: AD-MSCs were characterized using flow cytometry. Adult male Wistar rats were treated with/without AlCl3 and injected with/without AD-MSCs. After 5 days of AlCl3 ingestion and 4 weeks of subsequent AD-MSCs intraperitoneal injection, behavioral and molecular assessments were conducted. The Y-maze alternation test was used to test spatial learning of rats. Nestin gene expression was evaluated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The presence of amyloid plaque in the cortex and the hippocampus was evaluated through Congo red staining.Results: AD-MSC-like cells expressed the MSC markers CD90, CD73 and CD105. The Y-maze alternation result for rats treated with AlCl3 and AD-MSC-like cells was significantly higher compared with rats treated with AlCl3 only. Nestin gene expression was significantly higher in rats treated with AlCl3 and AD-MSC-like cells compared to those treated with AlCl3 only. After AD-MSC-like cells treatment, the Congo red staining results of rat’s cortex and hippocampus were significantly decreased.Conclusion: The findings suggest that AD-MSC-like cells possess therapeutic potential in restoring neural plasticity, amyloid plaque clearance and warrant further investigation for AD treatment. This study contributes to the emerging field of stem cell therapy for neurodegenerative diseases by highlighting the promise of AD-MSCs.Keywords: Alzheimer's disease, adipose-derived mesenchymal stem cells, neural plasticity, congo red staining, stem cell therapy
Clinical characteristics of maxillofacial fracture patients in Dr. M. Djamil General Hospital Padang Hafiz, Al; Maidatuz Zahra, Agnesia; Mulyani, Henny; Huriyati, Effy; Revilla, Gusti; Zhuhra, Rahma Tsania
Oto Rhino Laryngologica Indonesiana Vol. 54 No. 2 (2024): VOLUME 54, NO. 2 JULY - DECEMBER 2024
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32637/orli.v54i2.661

Abstract

Background: Maxillofacial fracture consists of several types of fractures depending on their location. Traffic accidents are the most common cause of these cases. The choice of management given in maxillofacial fractures is divided into immediate and planned delayed management, depending on the condition of the injured tissue. Purpose: To describe the clinical characteristics of maxillofacial fracture in the Department of Otorhinolaryngology Faculty of Medicine Universitas Andalas/Dr. M. Djamil General Hospital Padang, in 2020 to 2022. Method: A descriptive study with retrospective approach. The study was conducted by collecting data from the medical record section by using a total sampling technique (59 patients) from 2020 to 2022. Result: The highest cause was traffic accidents (49.2%), the most common findings were nasal bone fracture (44.1%), with operative management (82.4%). Conclusion: Maxillofacial fractures were most commonly experienced by the young age group and caused by traffic accidents, therefore driving safety and driver compliance should be more emphasized. Keywords: maxillofacial fracture, ORIF (Open Reduction Internal Fixation), septorhinoplasty, septoplasty, rhinoplasty ABSTRAK Latar belakang: Fraktur maksilofasial terdiri dari beberapa jenis fraktur tergantung lokasinya. Kecelakaan lalu lintas menjadi penyebab tersering dari kasus ini. Pemilihan tatalaksana yang diberikan pada fraktur maksilofasial dibagi menjadi tatalaksana segera dan tatalaksana lanjutan terencana, tergantung dari kondisi jaringan yang terluka. Tujuan: Untuk mengetahui karakteristik klinis pasien fraktur maksilofasial di Departemen THT-BKL FK Unand/RSUP Dr. M. Djamil Padang, dari 2020 sampai 2022. Metode: Penelitian ini bersifat deskriptif dengan pendekatan retrospektif. Penelitian dilakukan dengan pengambilan data di bagian rekam medik dengan teknik total sampling dan didapatkan sampel sebanyak 59 pasien pada tahun 2020 sampai 2022. Hasil: kelompok usia paling sering adalah 16-30 tahun (54.2%), dengan jenis kelamin terbanyak adalah laki-laki (76.3%), penyebab tersering adalah kecelakaan lalu lintas (4.2%), jenis fraktur tersering adalah fraktur tulang hidung (44.1%), dan tatalaksana paling sering adalah tatalaksana operatif (82.4%) Kesimpulan: Fraktur maksilofasial paling sering dialami oleh kelompok usia remaja dan disebabkan kecelakaan lalu lintas, oleh karena itu keselamatan dan kepatuhan berkendara perlu lebih diperhatikan dan ditingkatkan. Kata kunci: fraktur maksilofasial, ORIF (Open Reduction Internal Fixation), septorinoplasti, septoplasti, rinoplasti
HUBUNGAN NASAL INDEX DENGAN FUNGSI RESPIRATORI HIDUNG : SEBUAH TINJAUAN SISTEMATIS Advin, Tesya; Hafiz, Al; Revilla, Gusti
EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan Vol. 2 No. 1 (2025): EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan, Maret 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/empiris.v2i1.982

Abstract

Background : Nasal Index is one of the anthropometric parameters of the nose. Many studies have established the applicability of nasal index to categorize racial, gender and age variations. However, there are still few studies that have determined whether physiological differences are associated with variations in nasal index. The purpose of this study was to assess the relationship between nasal index with nasal respiratory function. Method : This research is a systematic review. The sources of databases used are Pubmed, Proquest, Science Direct, and BMC in the 2012-2022 period. Articles are selected based on predetermined eligibility criteria. The primary articles identified were screened using the PRISMA method, resulting in 10 studies included in the systematic review. Result : 4 out of 10 studies stated that nasal morphology generally affects nasal respiratory function. 3 studies found an association between nasal index and nasal air condition regulation, and 1 study found an association between nasal index and airflow and nasal resistance. The other 6 studies explained that there was no relationship between nasal index and nasal respiratory function, either in differences in airflow and nasal resistance or in the regulation of nasal air conditions. Conclusion : There is no significant relationship between nasal index and nasal respiratory function.
KARAKTERISTIK GEJALA KLINIS IBU HAMIL DENGAN INFEKSI COVID-19 DI RSUP DR. M. DJAMIL PADANG Milano, Ryan Suheil; Defrin, Defrin; Firdawati, Firdawati; Aladin, Aladin; Russilawati, Russilawati; Revilla, Gusti
EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan Vol. 2 No. 1 (2025): EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan, Maret 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/empiris.v2i1.1018

Abstract

Background: The SARS-CoV-2 virus easily spreads globally in a short period. Pregnant women are one of the vulnerable groups who can be infected with COVID-19, they have a risk of complications during pregnancy Objective: This research aims to identify the traits of expectant mothers with COVID-19 infection at Dr. M. Djamil Padang. Methods: This form of research uses retrospective descriptive approach, utilizing a comprehensive sampling and relying on secondary data sources. The research was conducted at Dr. M. Djamil Padang Hospital by taking data on pregnant women with confirmed COVID-19 infection in 2020-2021. Samples that met the inclusion and exclusion criteria were 302 cases Result: The results of this study showed the most gestational age was 3rd trimester (96.0), no clinical symptoms (58.6%), with the highest pregnancy outcomes being mature (72.5%), the highest birth weight babies being 2500-4000 grams (81,1%) and the highest maternal and infant outcomes being live mothers and babies ( 87.7%). Conclusion: This study concludes is that most pregnant women with COVID-19 infection at Dr. M. Djamil Padang in 2020-2021 with characteristics of third trimester gestational age, no clinical symptoms, mature pregnancy outcome, weight normal birth and high maternal and infant survival rates
Effect of Human Bone Marrow Mesenchymal Stem Cells on Burn Healing in Granulation Tissue Formation in Diabetic Rats Revilla, Gusti; Saputra, Deddy; Nurhasanah, Dini
Jurnal Kesehatan Andalas Vol. 11 No. 3 (2022): Online November 2022
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v11i3.2089

Abstract

Burns with diabetes mellitus can interfere with the wound-healing process. Mesenchymal stem cells have been studied to accelerate the healing of burns, one of which originates from the bone marrow. Objective: To determined the effect of human bone marrow mesenchymal stem cells (hBM-MSCs) on burn wound healing in granulation tissue formation in Diabetes Mellitus rats. Methods: This study was an experimental study with a post-test-only control group design, using 30 rats divided into two groups, the control group Phosphate Buffer Saline (PBS) and the treatment group (hBM-MSCs). Mice were induced by alloxan to cause hyperglycemia. Burns are made using a heated plate. Tissue collection was carried out after the termination of the experimental animals on the 3rd, 7th and 14th days and then histological preparations were made to assess the formation of granulation tissue. Data were analyzed using the two-way ANOVA test. Results: There was an increase in the number of macrophages, fibroblasts and new blood vessels after being given hBM-MSCs. On day 14 neutrophils and lymphocytes on day 3, there was no increase. Statistical analysis showed a significant increase in lymphocytes, macrophages, fibroblasts and new blood vessels. Conclusion: Human bone marrow mesenchymal stem cells can increase the formation of granulation tissue by increasing the number of macrophages, fibroblasts and new blood vessels in diabetic rats.Keywords: burn wound,  diabetic rat, human bone marrow mesenchymal stem cells, granulation tissue
Efek Human Bone Marrow Mesenchymal Stem Cells Terhadap Deposisi Kolagen Pada Kulit Tikus Luka Bakar Full Thickness Diabetes Melitus Aljassri, Resti Karunia; Revilla, Gusti; Zulfadli Syahrul, Muhammad; Yerizel, Eti; Saputra, Deddy; Tofrizal, Tofrizal
Jurnal Ilmu Kesehatan Indonesia Vol. 6 No. 1 (2025): Maret 2025
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jikesi.v6i1.1333

Abstract

Latar Belakang: Kulit berfungsi sebagai pelindung tubuh manusia dan rentan mengalami kerusakan, salah satunya luka bakar. Luka bakar disebabkan oleh trauma panas atau dingin yang memiliki risiko morbiditas dan mortalitas yang tinggi. Diabetes melitus dapat menghambat penyembuhan luka bakar dengan memperpanjang inflamasi, mengganggu proses re-epitelisasi, sintesis, dan proliferasi fibroblas. Human Bone Marrow Mesenchymal Stem Cells (hBM-MSCs) dapat mempercepat penyembuhan luka bakar pada kulit. Objektif: Penelitian ini bertujuan untuk mengetahui efek hBM-MSCs terhadap deposisi kolagen pada penyembuhan luka bakar dengan diabetes melitus. Metode: Penelitian ini dilakukan secara post-test only control group design. Penelitian ini menggunakan 30 blok parafin yang berisikan jaringan kulit luka bakar full thickness tikus diabetes melitus yang diterminasi pada hari ke-3, ke-7, dan ke-14. Terdiri dari kelompok kontrol dan perlakuan. Jaringan kulit dibuatkan preparat histologis dengan pewarnaan Picro Sirius Red untuk menilai deposisi kolagen. Data dianalisis menggunakan uji T-Independent. Hasil: Hasil penelitian menunjukkan terjadi peningkatan deposisi kolagen pada kulit tikus luka bakar full thickness diabetes mellitus setelah diberikan hBM-MSCs. Analisis statistik deposisi kolagen pada hari ke-3 dan ke-7 tidak menunjukkan peningkatan yang signifikan, sedangkan hari ke-14 terlihat peningkatan yang signifikan. Kesimpulan: hBM-MSCs berpotensi meningkatkan deposisi kolagen pada kulit luka bakar tikus diabetes melitus.