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All Journal Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Jurnal Kedokteran Brawijaya Jurnal Ilmiah Kursor Indonesian Journal of Urology Berkala Ilmu Kesehatan Kulit dan Kelamin Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Cancer Majalah Obstetri dan Ginekologi Medical Laboratory Technology Journal Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Manajemen Kesehatan Yayasan RS.Dr. Soetomo Majalah Patologi Indonesia Prosiding Seminar Nasional Multidisiplin Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Majalah Biomorfologi (Biomorphology Journal) Indian Journal of Forensic Medicine & Toxicology INDONESIAN MIDWIFERY HEALTH AND SCIENCES JOURNAL Indonesia Journal of Physical Medicine and Rehabilitation Indonesia Journal Physical Medicine and Rehabilitation Cermin Dunia Kedokteran Indonesian Journal of Cancer Jurnal Pengabdian Masyarakat Bhinneka The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Jurnal Pengabdian Masyarakat Bangsa Enrichment: Journal of Multidisciplinary Research and Development Jurnal Widya Medika
Willy Sandhika
Departemen/ SMF Patologi Anatomi , Fakultas Kedokteran Universitas Airlangga/ RSU Dr. Soetomo, Surabaya, Indonesia
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Journal : Indonesian Journal of Urology

 1 
TRANSPLANTATION OF ALLOGENIC ADIPOSE-DERIVED STEM CELL IMPROVE URETHRAL MUSCLE-COLLAGEN RATIO AFTER VAGINAL DISTENSION Yudiana, I Wayan; Hardjowijoto, Sunaryo; Alif, Sabilal; Purwati, Purwati; Sandhika, Willy; Budiono, Budiono
Indonesian Journal of Urology Vol 21 No 2 (2014)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v21i2.30

Abstract

Objective:To investigate the effects of allogenic adipose-derived stem cell (ADSC) transplantation on urethral tissue regeneration after vaginal distension in a rat model.Material & Method:32 female wistar rats underwent vaginal distension (VD) for 4 h. Subsequently, PKH-labeled ADSCs (2 x 105 in 200 µl PBS, ADSC group, n = 18) or PBS (200 µl, placebo group, n = 18) were injected into periurethral tissue. Two and four weeks following transplantation (ADSC or PBS), voiding behavior (frequency) and muscle-collagen ratio of urethral tissue were measured to evaluate urethral sphincter regeneration. Data were analysed by paired-t test, one-way ANOVA and post hoc LSD.Results:Mean voiding frequency was significantly different in the ADSC group at two weeks (p<0.0001), but not significantly different at four weeks (p=0.448) when compared with the placebo group.Masson’s trichrome staining revealed that the muscle-collagen ratio of urethral tissue was not significantly different between study groups at two or four weeks (p=0.053 or p=0.166 respectively). Muscle-urethral ratio was more specific showing a significant difference at two weeks (p=0.043). There were significant differences about muscle-collagen or muscle-urethral ratio between control and placebo groups (p<0.05), whereas between control and ADSC groups no significant difference was observed (p>0.05).Conclusion:ADSC transplantation promotes urethral muscle-collagen ratio with development of striated muscle after vaginal distension, so that can improve voiding behavior in a rat model.Keywords:Adipose-derived stem cell, vaginal distension, rat-SUI model, muscle-collagen ratio, voiding stain on paper.
ADIPOSE-DERIVED STEM CELL TRANSPLANTATION ON URETHRAL MICROVESSEL AND VOIDING FUNCTION Mahadi, Endi Prilansa; Hardjowijoto, Sunaryo; Alif, Sabilal; Purwati, Purwati; Sandhika, Willy; Budiono, Budiono
Indonesian Journal of Urology Vol 22 No 1 (2015)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v22i1.101

Abstract

Objective: To examine the effects of adipose-derived stem cell (ADSC) transplantation on urethral tissue microvessel and voiding function on artificial stress urinary incontinence (SUI) in rats. Material & methods: 25 of 32 female wistar rats underwent vaginal distension as animal-SUI model. ADSCs were isolated from the peri-ovary fat, examined for stem cell properties, and labeled with PKH-2. Seven rats without vaginal distension and periurethral ADSCs injection as control. Twelve rats received periurethral injection of phosphate buffer saline as plasebo and 13 rats received periurethral injection of ADSCs. 14 and 28 days later, voided volume was meassured with voided stained on paper and microvessel density was meassured with immunohistochemical analysis (factor VIII R-Ag). Results: Voided volume analysis showed that rats in the plasebo group had abnormal voided volume compare to normal rats in day 14 (168.5 ± 119.05l vs 621.21 ± 283.85l; p < 0.05), and insignificant improvement of voided volume compare to normal rats in day 28 (852.09 ± 626.7l vs 868.49 ± 578.0l; p > 0.05). While in the ADSCs group only showed significant improvement of voided volume compare to abnormal rats in days 14 (379.35 ± 191.74l vs 228.18 ± 56.26l; p < 0.05). Immunohistochemical analysis showed that microvessel density higher in the ADSCs group compare to plasebo group in days 28 (12.86 ± 2.5 vs 9.50 ± 1.64; p < 0.05). Conclusion: ADSCs transplantation promotes improvements voided volume and urethral microvessel in the rat-SUI model. Keywords: Adipose-derived stem cell, vaginal distension, voided stained on paper, microvessel density.
EFFECT OF PERIURETHRAL INJECTION ADIPOSE-DERIVED STEM CELLS IN NERVE HEALING AND VOIDING BEHAVIOR FOR THE TREATMENT OF STRESS URINARY INCONTINENCE IN A RAT MODEL Rizaldi, Fikri; Purwati; Sandhika, Willy; Budiono; Hardjowijoto, Sunaryo
Indonesian Journal of Urology Vol 28 No 1 (2021)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v28i1.713

Abstract

Objective: To investigate whether ADSCs are capable of nerve repair in stress urinary incontinence. Material & Methods: Thirty rats were allocated to five groups for receiving a periurethral injection with Phosphate Buffer Saline (PBS) (B1,B2) and Adipose-derived Stem Cells (C1, C2). Functional assessments with Voided Stain on Paper (VSOP) Method were reassessed at 2 and 4 weeks after injection. The rat SUI model was generated by Vaginal Distention (VD). The regeneration of peripheral nerve was evaluated by PGP 9.5 immunohistochemical staining. Results: Results revealed significant peripheral nerve differences between ADSC group and other groups in 28 days using PGP 9.5 staining. Our functional assessment, Voided Stain on Paper (VSOP), showed that ADSC group had significant improvement in 14 days; unfortunately, our 28 days improvement was not substantial. Conclusion: Periurethral injection of ADSCs improves urine pattern in SUI-induced rats and increase the amount of peripheral nerve. This method may be a potential strategy for SUI treatment.
Co-Authors A.A. Ketut Agung Cahyawan W Afif Nurul Hidayati, Afif Nurul Agung Dwi Wahyu Widodo Agustin Nurliani Aisha Grayli Cahyani Alief Yudo Astuti Alphania Rahniayu Anny Setijo Rahaju, Anny Setijo Ariani, Grace Aries Sasongko Astindari Astindari Astindari Astindari, Astindari Ayu Tyasmara Bagus Haryo Kusumaputra, Bagus Haryo Baiq Ratna Kumaladewi Bernadetta Jonan Betty Agustina Tambunan Brama Rachmantyo Budiono Budipramana, Vicky Sumarki Cita Rosita Sigit Prakoeswa Densy Violina Harnanti Dian Eskaningrum Djoni Susanto Dwi Murtiastutik DYAH FAUZIAH, DYAH Endi Prilansa Mahadi Erlina Erlina Faizah, Zakiyatul Fanny Gunawan Fertilita, Soilia Firda Azizah Gatut Hardianto, Gatut Gilda Hartecia Gondo Mastutik Grace Ariani Hartanto, Felix Hartecia, Gilda Heny Arwati Heriyawati, Heriyawati Heryawati Heryawati I Wayan Yudiana Ibrahim, Azarya Ida Septika Wulansari Ilmiah, Khafidhotul Imam Susilo INDROPO AGUSNI Irene Lingkan Parengkuan Irmadita Citrashanty, Irmadita Isnin Anang Marhana Iswinarno Doso Saputro iwan Syarif Jimmy Hadi Widjaja Jonan, Bernadetta Junita Jeanne Paliman Kartika Arum Wardani Kholida Nur Aini Kiki Apnita Sari Kintan Putri KUSUMASTUTI, ETTY HARY LaleMaulin Prihatina Linda Astari, Linda M. Yulianto Listiawan Mappamasing, Hasnikmah Marina Rimadhani Marina Rimadhani Martha Kurnia Kusumawardani Maylita Sari Maylita Sari, Maylita Meita Ardini Pratamasari, Meita Ardini Muhammad Miftahussurur Nanda Amalia Ramadhanti Navisa, Claudia Clary Nidya Ulfana Tsania Nila Kurniasari Novia Indriyani Adisty Nurina Hasanatuludhhiyah Nurul Wiqoyah, Nurul Poernomo, Adinda Sandya PUNGKY MULAWARDHANA, PUNGKY Purwati Purwati Purwati Puteri, Agnes Ilene Suprapto Qonitatillah, Ana R. Heru Prasetyo R.A. Astrid Putri Wandhita Rahel Yuana Sadikim Ratna Darjanti Haryadi Regina Martina Cilik Ridholia, Ridholia Rizaldi, Fikri Rulisiana Widodo Rusdamayanti Rusdamayanti Rwahita Satyawati Dharmanta Ryski Meilia Novarina Ryski Meilia Novarina, Ryski Meilia S.Pd. M Kes I Ketut Sudiana . Sabilal Alif Sari, Aditya Sita Sari, Festi Artika savitri, Lisa Sawitri Sawitri Sawitri Sawitri Sawitri Sjahjenny Mustokoweni, Sjahjenny Soilia Fertilita Sunarso Suyoso Sunaryo Hardjowijoto Suprabawati, Desak Gede Agung Tessy Badriyah, Tessy Theresia Fifi Judikristiani Tri Hartini Yuliawati Trisniartami Setyaningrum Tyasmara, Ayu Umi Choviva, Farida Ummi Maimunah Vinna Chrisdianti Widati Fatmaningrum Wilda Fitria Rachmadina Windya Tri Hapsari Winona May Hendrata Wiratama, Priangga Adi yasmin, dzakiyah Yusfita Evi Rosdiana