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Journal : International Journal of Health and Pharmaceutical (IJHP)

Antioxidant Activity Test Of Methanol Extract Of Gaharu (Aquilaria Malaccensis Lam.) Bark With Dpph (1,1 Diphenyl-2-Picrylhydrazyl) Method Ridwanto Ridwanto; Asep Trizaldi; Zulmai Rani; Anny Sartika Daulay; Haris Munandar Nasution; Dikki Miswanda
International Journal of Health and Pharmaceutical (IJHP) Vol. 3 No. 2 (2023): May 2023
Publisher : CV. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51601/ijhp.v3i2.123

Abstract

Gaharu (Aquilaria malaccensis Lam.) is one of the natural ingredients that can be used as an antioxidant. Antioxidants are compounds that can inhibit free radical reactions in the body. Free radicals have an impact on the pathogenesis of several diseases in humans due to oxidative stress in cells. The purpose of this study was to determine the class of secondary metabolites of agarwood bark and antioxidant activity and to compare the value of IC50 (Inhibitory concentrations) of the methanol extract of agarwood bark with the IC50 of vitamin C in the DPPH method using UV-Vis spectrophotometry. The methanol extract of agarwood bark was macerated using methanol, then determined the content of secondary metabolites of Simplicia. The determination of antioxidant activity was carried out on the methanol extract of agarwood bark with the addition of DPPH at various concentrations. The sample concentrations were 20, 40, 60, 80, and 100 µg/mL.. As a comparison, vitamin C was used with concentrations of 4, 8, 12, 16 and 20 µg/mL.. Then they calculated percent attenuation (% inhibition) and the value of IC50. The results of the screening on the methanol extract of agarwood bark contained chemical compounds such as flavonoids, saponins, and tannins. The determination of antioxidant activity was carried out using a UV-Vis spectrophotometer with the DDPH method. The results were obtained from the methanol extract of the bark of agarwood, which has antioxidant activity in the strong category with an IC50 value of 94.59 µg/mL.and vitamin C in the very strong category with an IC50 value of 22,11 µg/mL.
Co-Authors Afrizani, Afrizani Akbar, Windi Hari Ali Djamhuri Alviana, Liya Anugrah, Bayu Asep Trizaldi Asparyzha, Rhyzha Azri, Atika Cici Andriani Daeng Elysa Putri Mambang Dalimunnthe, Gabena Indrayani Dalimunthe, Gabena Indriyani Dikki Miswanda Dina Agustia Parlin Dina Suciati Saragih Eva Fransiska Fadillah, Nike Fathur Rahman Fatur Rahman fatur Rahman, fatur Fithri Pulungan, Ainil Fitri Mulyani, Fitri Gabena Indrayani Dalimunthe Hafizha, Putri Harahap, Siti Salimah Haris Munandar Nasution Hasanah, Qori Hasrul Abdi Hasibuan Hindri Syahputri Ihsan Fadhilah Leni Handayani Lubis, Minda Sari M. Naufal Rifqi M. Pandapotan Nasution Makhfirah Manik, Umi Chairani Mardhatillah, Wulan Maya Syafira Mayang Sari Ritonga Merani Phaustina Lumban Gaol Miranza, Nona Muhammad Amin Nasution Muhammad Hizbullah Muhammad Wahyudi Munthe, Ariska Nabilla, Alfira nasution, Alfina Tri Utami Nasution, Kartika Zsaskia Nasution, Nur Sahadah Nia Novranda Pertiwi Nst, Haris Munandar Nur Hanifah Nur'ain Harahap Nurhayati Nurhayati Nurul Salsa Abya Ritonga Pasaribu, Mesi Wilia Afrima Pasaribu, Mesi Willia Afrima Putri Intan Sari Putri Theresia Harianja Rafita Yuniarti Rahayu Rahayu Rahmadani Rahmadani Rahmah, Ruhiya Rahmasari, Siti Rahmi Ayusasmita Gultom Retno Sekarini Rezky , Deswita Ina Ricky Andi Syahputra Ridwanto Ridwanto Ridwanto Ridwanto Ridwanto Rika Yuliana, Rika Risma Fauziah Pasaribu Rizki, Rahmad Rosaldi, Hikmah Rossa, Aliffa Sagita Marina Simatupang Sandika, M Teguh Sasnita, Merida Sekarini, Retno Shilvia, Fatin Siagian , Anggi Yani Sinaga, Novita Yulianti Sri Murni Supiyani, Supiyani Syahfitri , Adelya Syahputra, Ricky Andi Syalsabila Putri Tuzzahra, Safira Yayuk Putri Rahayu Yayuk Putri Rahayu Yuniarti , Rafita Yuniarti, Rafita Zulmai Rani