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All Journal HAYATI Journal of Biosciences MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Ilmu Pertanian Indonesia Jurnal Silvikultur Tropika (JST) MEDIA KONSERVASI FORESTA Jurnal Penelitian Hutan dan Konservasi Alam Jurnal Penelitian Hutan Tanaman JURNAL PEMULIAAN TANAMAN HUTAN AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat ANNALES BOGORIENSES Jurnal Ilmu dan Teknologi Kayu Tropis Jurnal Teknik Mesin Indonesia JESBIO : Jurnal Edukasi dan Sains Biologi ALMUJTAMAE: Jurnal Pengabdian Masyarakat Jurnal Sylva Lestari Repong Damar: Jurnal Pengabdian Kehutanan dan Lingkungan Jurnal Pengabdian Pada Masyarakat Policy Brief Pertanian, Kelautan, dan Biosains Tropika Journal of Fisheries & Marine Annales Bogorienses
Ulfah Juniarti Siregar
Departement Of Silviculture, Faculty Of Forestry And Environment, IPB University, Bogor 16680, Indonesia
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Optimization of Somatic Embryogenesis Induction of Cassava (Manihot esculenta Crantz) Susanti, Idha; Suharsono, Suharsono; Widyastuti, Utut; Siregar, Ulfah Juniarti; Tjahjoleksono, Aris
Annales Bogorienses Vol. 21 No. 2 (2017): Annales Bogorienses
Publisher : BRIN

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Abstract

The embryogenesis (SE) has important role for genetic engineering of cassava (Manihot esculenta Crantz). However, the success of SE induction depend on plant growth regulator s (PGR)s and treatment enriched in induction media. This experiment tried to induce callus formation of cassava from several in vitro explants: immature leaf, apical bud, and internode; and to develop somatic embryogenesis of cassava in several media enriched with tyrosine and copper sulphate (CuSO4) added into media enrich with picloram as treatment. Different response of explants source to callus induction treatment from those three varieties in callus induction as well as friable callus formation were found in this experiment. The best medium to induce varied with variety; MS media supplemented 12 mg/L picloram + 0.5 mg/L CuSO4 was the best for “Adira 4” and half MS and half GD media supplemented 12 mg/L picloram + 100 mg/L tyrosine for “Malang 6”. All treatments resulted somatic embryo which developed indirectly and in morphologically normal somatic embryos
Co-Authors Adam, Satria Adi Jaya, Adi Adisti Permatasari Putri Hartoyo Aditya Nugroho Aditya Nugroho Agus Kholik Agus Setiyono Ahmad Faqih Andi Sukendro Anita, Vilda Puji Dini Arief Budiman Arief Budiman Arif Kurnia Wijayanto Ario Damar Aris Tjahjoleksono Bayu Winata Bayu Winata, Bayu Cartealy, Imam Civi Chairil Anwar Siregar Chairil Anwar Siregar Chairil Anwar Siregar Dede J Sudrajat Dede J. Sudrajat Dede J. Sudrajat Dede Jajat Sudrajat Dede Jajat Sudrajat, Dede Jajat DEDE SUDRAJAT Deden D. Matra Deden Derajat Matra DIMAS ANDRIANTO Djiono Djiono Djufri Djufri Dodi Nandika Dodik Ridho Nurrochmat Eko Agus Suyono Eko Agus Suyono Esti Nurianti Evy Damayanthi Farosandi, Naufal Hilmi Fifi Gus Dwiyanti Fitta Setiajiati Hamzah, Hamzah Hartati Hartati Hartati, N Sri Hasim Hasyyati Shabrina Henti Rosdayanti Herman Daryono Herman Daryono I Made Mayun Maha Diputra I Wayan Windra Nugraha Idha Susanti Idha Susanti Idqan Fahmi IPB, DGB Irdika Mansur Iskandar Z Siregar Iskandar Z. Siregar Iskandar Z. Siregar ISKANDAR ZULKARNAEN SIREGAR Istikorini, Yunik Iswanto Jumadin Sidabutar Lala M Kolopaking Laura Flowrensia Lilik Maslachah Lina Karlinasari Lucia Tri Suwanti, Lucia Tri M Agus Setiadi M Iqbal Maulana Ginting M.Pd S.T. S.Pd. I Gde Wawan Sudatha . Ma'mun Sarma Madani, Hilmi Naufal Mahendra Pujiyanto Matra, Deden Derajat Mochamad Donny Koerniawan Mochammad Donny Koerniawan Mohammad Basyuni N. Sri Hartati Nahrowi Noor Farikhah Haneda Noor Farikhah Haneda Nurianti, Esti Nurul Khumaida Oemijati Rachmatsyah Oktariza, Wawan Pramisari, Yunita Pramisasi, Yunita Prijanto Pamoengkas PURNAMA HIDAYAT Purwiyatno Hariyadi Puti Awali Saimima Ramadhan, Rafli Ranny Dwita Olivia Rifana, Haikal Zaky Rita Rahmawati Roisatuz Zakiyah Satriawan, Handi Sona Suhartana Sona Suhartana Sri Purwaningsih, Sri Sri Wilarso Budi Suci Rahayu Sudradjat Sugianto Sugianto Suharsono Suharsono Suharsono Suharsono Sumiati Supatmi Supatmi Suria Darma Tarigan Syamsidah Rahmawati, Syamsidah Tedi Yunanto Tedi Yunanto UTUT WIDYASTUTI Utut Widyastuti Vilda Puji Dini Anita Widiyatno, Thomas Valentinus Wirrahma . Yosie Syadza Kusuma Zhaza Afililla Zidan, Mochammad