Claim Missing Document
Check
Articles

Phosphate Solubilizing and Antifungal Activity of Root Endophyte Isolated from Shorea leprosula Miq. And Shoreal selanica (DC) Blume Safinah Surya Hakim; Sri Wilarso Budi; Maman Turjaman
Jurnal Manajemen Hutan Tropika Vol. 21 No. 3 (2015)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (842.269 KB) | DOI: 10.7226/jtfm.21.3.138

Abstract

Fungal endophytes are fungi that lives within plant tissues without causing apparent disease. It is also suggested that these fungi have ability to enhance plant growth and plant resistancy against pest and disease. This research is a preliminary study about root fungal endophytes in dipterocarp since there are lack research concerning about this study focus. We examined root fungal endophyte  isolated from seedling of Shorea leprosula and Shorea selanica taken from Dramaga Experimental Forest, Bogor.  Furthermore, we also tried to find out the fungal potential ability to solubilize phosphate and suppres fungal pathogen by in vitro assay. Surface sterilization method was used to isolated fungal endophytes from root tissues. Trichoderma spirale, Velsalceae sp., Melanconiela ellisii, Chaetosphaeria callimorpha, and Trichoderma asperellum were isolated during this study. These fungi appear to have specific association between fungal species and host plant, but no evidence of fungal order-level specificiation in S. leprosula and S. selanica. In vitro test also suggested that root fungal endophyte Trichoderma spirale and Melanconiella elisii have potential ability to solubilize inorganic phosphate. In addition, this result also present that root fungal endophyte T. spirale and T. asperellum have the potential to inhibit pathogen fungi Fusarium sp.  
Application of Arbuscular Mycorrhizal Fungi and Soil Ameliorant on the Growth of Leucaena leucocephala in Limestone Post-mining Soil Media Siti Hanna Ghaida; Basuki Wasis; Sri Wilarso Budi
Jurnal Manajemen Hutan Tropika Vol. 26 No. 3 (2020)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.7226/jtfm.26.3.282

Abstract

Limestone mining has the potential into environmental damage that involve modify an ecosystem. The attempt that contrived to reduce the disturbances are rehabilitation. This research was conducted to examine the growth response of Leucaena leucocephala inoculated with AMF and soil ameliorant in a limestone post-mining soil. The design used was a split-plot design in a completely randomized design with 3 factors. The first factor was AMF inoculum (Daemonorops draco AMF and MycoSilvi), the second factor was organic fertilizer of compost, and the third factor was inorganic fertilizer. The variables used in this study expressed by height, diameter, biomass, root colonization, and nutrient absorption of the plant. The analysis showed that the combination of MycoSilvi and compost 7.5% gave best result of height, diameter, and biomass, with significantly increased by 962.67%, 899.41% and 1440.67% to control plant. It also gave best result of nutrient uptake N, P, and K, with significantly increased up to 17.64 g plant-1, 2.42 g plant-1, and 18.05 g plant-1. In general, AMF showed a good percentage of root colonization with an average 36.67-86.67%. Further research is needed to determine the response to the growth of seedlings planted in the field.
Optimalisasi kerja mycofer dengan augmentasi mikroorganisme tanah potensial dan asam humat untuk rehabilitasi lahan marginal dan terdegradasi di Indonesia Panca Dewi MH Karti; Sri Wilarso Budi R.; Noor F Mardatin
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 2 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1857.776 KB)

Abstract

Marginal and degraded lands in Indonesia are considerably extensive and include many kinds of soil, for instance acid soil and post mining soil. Efforts to overcome the problem of acid soils and post mining soil are  the use of biological fertilizer such as arbuscular mycorrhizal fungi (FMA), phosphate dissoving microorganism (MPP), and nitrogen fixer microorganism (MPN). The objective of this research was seeking new formulation of biological fertilizer which constitute a consortium between Mycofer with MPP, MPN, and humic acid which could increase its ability for supplying nutrients and   help to increase plant survival in less favorable environment. The first year research comprised the following research stages: (i),  Preparation of augmentation materials, namely multipilication of potential microbe (FMA, MPP and MPN) and humic acid ; (ii)  Formulation of biological fertilizer which constitute the consortium of Mycofer with MPP, MPN and humic acid. There were 6 formulas of biological fertlizer which were tested in this research, namely (a) Mycofer with addition of MPP isolates 1, 2 dan 3; (b) Mycofer with addition of Azospirilum isolates 1, 2 and 3; (c) Mycofer with addition of Rhizobium isolates 1, 2 and 3; (d) Mycofer with addition of humic acid; (e) Mycofer with addition of  MPP, Azospirilum and Rhizobium; (f) Mycofer with addition of MPP, Azospirilum, Rhizobium and humic acid; (iii) Test of  Mycofer plus formulation in post mining acid soil and latosol soil at laboratory scale with corn crop and Legum Cover Crop (LCC) which comprised (a) Centrosema pubesens (b)  Pueraria phaseoloides, and grasses which comprised (a) Panicum maximum , and (b) Setaria splendida in pots with 5 kg capacity for 3 months. In the second year research, the first factor was microbe treatment, which compriserd 4 levels, namely P1 (control), P2 (mycofer), P3 (mycofer with Azospirillum/Rhizobium) and P4 (mycoferd, Azopirillum/Rhizobium, and MPP); whereas the second factor was technological treatment which comprised 3 kinds of technolgy, namely T1 (SOP of gold mining), T2 (technology of soil amelioriation), and T3 (hydroseeding technology). Result of the first year research indicate that the five crops species tested gave different responds weather in latosol soil or in post mining soil.   In general, all plant species tested indicate that they not only need single microorganism but  need consortium of microorganism.  The plants were better growth  when inoculated with consortium of microorganism combined with humic acid both in latosol soil and post gold mining soil respectively. The second year research was conducted in post gold mining land of PT Aneka Tambang, in Pongkor, Bogor.  The results showed the tolerant crops that did not require technology and addition of microbe for their growth are Calopogonium muconoides and Setaria spendida whereas Brahiaria humidicola and Centrosema pubesescens only required treatment P3, P4 and P2. Pueraria phaseoloides and Panicum maximum were categorized as sensitive plants because they required additio of microbes and technology, namely treatments T3P3 an treatment T3P4.
Aplikasi Amelioran Tanah dan MycoSilvi pada Falcataria sp. dan Ochroma bicolor Rowlee. untuk Reklamasi Lahan Pascatambang Pasir Silika Khoryfatul Munawaroh; Sri Wilarso Budi; Prijanto Pamoengkas
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 3 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.25.3.334

Abstract

Open pit mining of silica sand causes some soil degradations and environmental impacts, such as lowering soil pH, lowering soil fertility, soil compaction, and toxicity of micronutrients such as Al, Fe, and Zn. The aim of this study was to analyze the soil properties of land post mining of silica sand and to analyze the growth response of Falcataria sp. and Ochroma bicolor by combination of soil ameliorant and MycoSilvi. The experimental design was split plot design of two treatment factors, MycoSilvi and soil ameliorant. The main plot is MycoSilvi that consist of two levels (MycoSilvi and without MycoSilvi). The subplot is soil ameliorant that consisted of six levels (compost 0 g, lime 0 g; compost 0 g, lime 1.56 g; compost 0 g, lime 3.12; compost 9 g, lime 0 g; compost 9 g, lime 1.56 g; compost 9 g, lime 3.12 g). The results showed that the soil ameliorant (lime 3.12 g and compost 9 g) and MycoSilvi was the best treatment to increase height, diameter, biomass, and mycorrhizal colonization of Falcataria sp. and O. bicolor. Keywords: MycoSilvi, reclamation, soil ameliorant
Ketergantungan Tiga Jenis Tanaman Kehutanan terhadap Mikoriza pada Media Tanah Bekas Tambang Pasir Silika Putri Aurum; Sri Wilarso Budi; Prijanto Pamoengkas
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 2 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (562.488 KB) | DOI: 10.18343/jipi.25.2.309

Abstract

One effort to support the success of reclamation and rehabilitation of ex-silica sand mines is to improve the quality of seedling planted with mycorrhizal inoculation. Each plant has a mycorrhizal dependency which varies depending on the plant species and the level of soil fertility. This study aimed to analyze the mycorrhizal dependency of Kadam (Anthocephalus cadamba), Red Sengon (Albizia chinensis), and Kaliandra (Calliandra calothyrsus) grown on soil growing media from post silica sand mining and amended with compost and lime. The study used completely randomized design with a split plot design consist of two factors, first factor was mycorrhizal inoculation in the form of MycoSilvi inoculum, consisting of two levels and the second factor was soil ameliorant in the form of lime and compost consisting of six levels, each treatment was repeated five times. The results showed that the treatment of lime and MycoSilvi addition showed the highest percentage of mycorrhizal dependency on Kadam, Red Sengon and Kaliandra seedlings, which were 76, 62, and 59% respectively. The addition of lime and compost decreased the degree of relative mycorrhizal dependency of three plants species tested, in line with increasing available P in soil growth medium. Keywords: mycorrhizal dependency, MycoSilvi, post mined soil, soil ameliorant
Plant Diversity in Different Land Use Types at The Peat Hidrological Unit (PHU) of Mendahara – Batanghari River, Jambi Province Aji Nuralam Dwisutono; Sri Wilarso Budi; Istomo Istomo
Media Konservasi Vol 24 No 2 (2019): Media Konservasi Vol. 24 No. 2 Agustus 2019
Publisher : Department of Forest Resources Conservation and Ecotourism - IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (537.221 KB) | DOI: 10.29244/medkon.24.2.141-151

Abstract

The characteristics of tropical peatlands are still able to form a high diversity of plants. Conversion of tropical peatlands affects the composition of plants. The aim of this study was to find out effect of land use changes to the composition and diversity of plants in the Peat Hydrological Unit (PHU) Mendahara - Batanghari River. The research was conducted in three land use categories, namely secondary forest, coffee plantation, and oil palm plantation (subdivided into oil palm plantation 1 and oil palm plantation 2). In each study location, sample lane 20 m x 200 m were made. Overall, we found 77 species of plants. The results showed   number of plant species decreased due to changes of land use. There are 51 - 53 species of plants in secondary forest areas (out of a total of 58 species) that are not found in oil palm and coffee plantations areas. Differences in composition were also shown in the low value of community similarity (<50%). In the oil palm and coffee plantation areas, plant communities tend to be dominated by pioneer plants such as Melicope lunu-ankenda, Coffea liberica, Macaranga triloba, and Melastoma malabathricum. Secondary forest was dominated by plants species that characterize peatlands such as Tetramerista glabra, Parastemon urophyllus, Knema percoriacea, Litsea costalis var. nidularis and Madhuca motleyana. Changes in land use also reduce the level of diversity (H 'and R) at various levels of growth. Whereas in the oil palm and coffee plantation areas tend to form uniform stands (indicated through index E which describes the abundance distribution in community and index C which describes the dominance of species). Generally, the distribution pattern of plants is clumped. Uniform distribution was found in K. percoriacea and L. costalis var. nidularis. Keywords: land use changes, peatland characteristics, plant composition, plant diversity
Diversity of Arbuscular Mycorrhizae Fungi from Rhizosphere of Daemonorops draco Blume in Jambi: STATUS FUNGI MIKORIZA ARBUSKULA (FMA) PADA RIZOSFER JERNANG (Daemonorops draco Blume) DI JAMBI Betty Purwati; Sri Wilarso Budi; Basuki Wasis
Media Konservasi Vol 24 No 3 (2019): Media Konservasi Vol. 24 No. 3 Desember 2019
Publisher : Department of Forest Resources Conservation and Ecotourism - IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.473 KB) | DOI: 10.29244/medkon.24.3.261-268

Abstract

Tujuan dari penelitian ini adalah untuk mengkaji keanekaragaman Fungi Mikoriza Arbuskula (FMA) pada rizosfer jernang di Jambi. Teknik pengambilan sampel tanah dan akar dilakukan dengan metode proporsional. Pengambilan sampel tanah dilakukan secara komposit pada kedalaman 0-20 cm dan 20-40 cm, contoh tanah diambil dari rizosfer jernang sebanyak 500 g pada setiap batang. Spora diisolasi dengan tehnik tuang saring basah dan dilanjutkan dengan sentifugasi, lalu kepadatan sporanya diukur dan diidentifikasi. Hasil identifikasi menunjukkan rata-rata jumlah spora adalah 106,39-209,46 spora per 20 g tanah. Kolonisasi akar berada pada kisaran 39,25%-64,25%. Keanekaragaman FMA menunjukkan 48 tipe spora FMA yang terdiri dari 31 tipe Glomus, 9 tipe Acaulospora, 7 tipe Scutellospora, dan 1 tipe Gigaspora. Glomus memiliki tingkat penyebaran tertinggi di masing-masing kedalaman. Kelimpahan relatif Glomus di kedua kedalaman sebesar 100 %. Frekuensi relatif pada Glomus juga mendominasi pada kedalaman tanah 0-20 cm sebesar 92,27%, dan tanah pada kedalaman 20-40 cm sebesar 95,05%. Kata kunci: FMA, Daemonorops draco Blume, Jambi
This research aimed to study status of arbuscular mycrorrhizal fungus (AMF) in mangrove and coastal forest post-tsunami disaster, and to study the relationship of soil phosphorus content and number of AMF spores and AMF infection of mangrove and coastal forest post tsunami disaster.  Research results show that there were variability in spore numbers in the field studied.  The spore numbers in mangrove forest were found as 359, 235, 118 and 160 spores per 50 g of soil in Meulaboh, Banda Aceh, Pid AAboe B. Saidi; Sri Wilarso Budi R.; Cecep Kusmana
Forum Pasca Sarjana Vol. 30 No. 1 (2007): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This research aimed to study status of arbuscular mycrorrhizal fungus (AMF) in mangrove and coastal forest post-tsunami disaster, and to study the relationship of soil phosphorus content and number of AMF spores and AMF infection of mangrove and coastal forest post tsunami disaster.  Research results show that there were variability in spore numbers in the field studied.  The spore numbers in mangrove forest were found as 359, 235, 118 and 160 spores per 50 g of soil in Meulaboh, Banda Aceh, Pidie and Nias respectively.  Meanwhile, in coastal forest were 587, 297, 265 and 158 spores per 50 g in Meulaboh, Banda Aceh, Pidie and Nias respectively.  The root of Rhizophora sp.  and Casuarina equisetifolia were colonized by AMF both in mangrove and coastal forest.  Number of spores were negatively correlated to soil phosphorus content, however, there were tendency of positive correlation between root infection and soil phosphorus content.   Key words: coastal forest, mangrove forest, mycorrhiza
PERANAN RIZOBAKTERI DAN FUNGI MIKORIZA ARBUSKULAR DALAM MENINGKATKAN EFISIENSI PENYERAPAN HARA SORGUM MANIS (Sorghum bicolor L. Moench) Bedah Rupaedah; Iswandi Anas; Dwi Andreas Santosa; Wahono Sumaryono; Sri Wilarso Budi
Jurnal Ilmu Tanah dan Lingkungan Vol 16 No 2 (2014): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (628.403 KB) | DOI: 10.29244/jitl.16.2.45-52

Abstract

Efisiensi pemakaian pupuk kimia dapat didefinisikan berdasarkan aspek agronomi. Secara umum, semakin banyak hara yang diserap tanaman untuk meningkatkan hasil panen, maka tingkat efisiensi semakin tinggi. Pelacakan jumlah hara yang dapat diserap tanaman dalam meningkatkan produksi adalah komponen kunci untuk mengukur efisiensi hara. Percobaan lapangan dilakukan untuk menilai peranan rizobakteri dan fungi mikoriza arbuskular (FMA) pada berbagai konsentrasi pupuk kimia pada budidaya sorgum manis (Sorghum bicolor L. Moench). Dua galur rizobakteri (Mycobacterium senegalense 73LR dan Bacillus firmus 80JR) serta dua galur FMA (Gigaspora sp. 40MDL dan Glomus sp. 38MDL) digunakan sebagai inokulan dengan penambahan beberapa variasi konsentrasi pupuk kimia. Bobot biomasa, kandungan gula, serapan fosfor, kalium dan nitrogen dianalisis. Inokulasi rizobakteri mampu meningkatkan tinggi tanaman dan kandungan P pada taraf p<0.01, sedangkan bobot biomassa, kandungan gula, serapan P dan K pada taraf p<0.05. Inokulasi FMA saja berpengaruh nyata terhadap bobot biomassa dan kandungan N pada taraf p<0.01, sedangkan kandungan gula pada taraf p<0.05. Sementara itu, interaksi rizobakteri dan FMA mampu meningkatkan bobot biomassa, kandungan dan serapan K, serta efisiensi penyerapan hara N. Secara keseluruhan pengaruh inokulasi rizobakteri, FMA dan pupuk kimia serta interaksi faktor-faktor tersebut dapat meningkatkan bobot biomasa, kandungan gula, serapan P dan K, serta efisiensi penyerapan hara N oleh tanaman sorgum manis. Dengan demikian, pemakaian rizobakteri dan FMA berpotensi meningkatkan efisiensi penggunaan pupuk kimia, sehingga diharapkan dapat mengurangi biaya produksi budidaya sorgum manis.
Application Arbuscular Mycorrhizal Fungi on Desmodium ovalifolium in Post mining Soil Sri Muryati; Irdika Mansur; Sri Wilarso Budi
Jurnal Perbenihan Tanaman Hutan Vol 5, No 1 (2017): Jurnal Perbenihan Tanaman Hutan
Publisher : Forest Tree Seed Technology Research & Development Center (FTSTRDC)/ Balai Penelitian dan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (849.867 KB) | DOI: 10.20886/bptpth.2017.5.1.35-50

Abstract

Mining activities results on environmental degradation and finally on ecosystem damage. Legume cover crop forming symbiosis with beneficial soil microorganism is widely well known to be used for degraded land reclamation such as in post mining area. Desmodium spp. is one of legume cover crop forming symbiosis with arbuscular mycorrhizal fungi (AMF) and rhizobium symbiosis. This study aimed to analyse the growth respond D. ovalifolium that has inoculated AMF from rhizosfer of 4 types of Desmodium spp. There were two factors that are AMF inoculation and media treatment, were applied: with or without inoculation of AMF, and with or without applications of compost. Inoculation of AMF and compost application increased height of O. sumatrana two week after planting (WAP). Single inoculation of AMF into desmodium spp. improved height variables of stem significantly, total biomass, sprout biomass, and  number of postule roots as well. Combination of compost and post mining soil had the highest increased in height 2 WAP and roots biomass of Desmodium spp. This research implies that AMF application is an important key for effective methods in improving plant grow in post mining area. 
Co-Authors , Hariyadi AAboe B. Saidi Abdurrani Muin Adisti Permatasari Putri Hartoyo Agustina Puspita Dewi Aji Nuralam Dwisutono Akhir, Jumadil Akhmad Wahyudi Al Muzahiq, Hizbullah Allaily Allaily Andi Sukendro Ani Kurniawati Arif Budi Setiawan Arifandi, Candra Pradana Arum Sekar Wulandari Atang Sutandi Bambang S. Purwoko Baskoro Rochaddi Basuki Wasis Bayu Winata, Bayu Bedah Rupaedah Berry Oktavianto Betty Purwati Cahyo Wibowo Cahyo Wibowo Ceng Asmarahman Christina, Fiona Corryanti . Corryanti Corryanti Dadan Mulyana Daniel Happy Putra Dida Syamsuwida Didy Sopandie Dona Octavia DWI ANDREAS SANTOSA Dwi Puspasari Arum Ramadhani Elis Nina Herliyana Endah Retno Palupi Endang Suhendang Eny Widajati Erdy Santoso Erdy Santoso Erlina Rahmayuni, Erlina Fadhlurrahman, Muhammad Miftah Faisal Al Asad Fatimah Nur Istiqomah Febrina, Ayudia Fiona Christina Fransisca Yosina Regina Luturmas Gunawan Gunawan Hamim Hamim Harus, Johanes S Herman, Welly Heru Bagus Pulunggono Husna Husna Husna Husna Hutami Indah Pertiwi Ida Rosita Ida Rosita Ikbal . Ikbal Ikbal Imam Gozali Imam Wahyudi Indrawan, Imam Wahyudi Intan Fajar Kemala Irdika Mansur Irmanida Batubara Iskandar . Iskandar Iskandar Iskandar Iskandar Iskandar M. Lapanjang Iskandar Z Siregar ISKANDAR ZULKARNAEN SIREGAR Istomo . Iswandi Anas JAYANI, FARADILA MEI Jenny Rumondang Jenny Rumondang Jumadil Akhir Kartika Megawati Kartini Kramadibrata Kartini Kramadibrata Khiorunnisa Rizki Dwi Jayati Khoryfatul Munawaroh Kurniati Kurniati Lina Karlinasari Luluk Setyaningsih Maman Turjaman Maman Turjaman Maman Turjaman Maman Turjaman Maman Turjaman Maryani Fatimah Putri Hardhani Maya Melati MEITY SURADJI SINAGA MEITY SURADJI SINAGA Meli Nurdiani Mohammad Agus Salim Mohammad Agus Salim Salim Muhammad Helmi Muttaqin, Zainal MZ, Karina Nelly Fridayanti Noor F Mardatin NUNANG LAMAEK MAY Nurheni Wijayanto Octaverina, Shinta Kartika Panca Dewi MH Karti Prijanto Pamoengkas Puspitasari, Sri Putri Aurum Putri Aurum Rahmatia, Citra Rahmawati Rahmawati Rumondang, Amandita Lintang Sabti Indah Purwanti Safinah S. Hakim S Hakim Safinah Surya Hakim Saridi Satriyas Ilyas Silitonga, Exsaudina Siti Hanna Ghaida Sri Muryati Sri Muryati Sri Muryati Sri Muryati Sudirman Yahya SUDIRMAN YAHYA Suharti, Sri Surono Tedi Yunanto Teuku Tajuddin Tirsa Eka Saputri Ulfah Juniarti Siregar Wahono Sumaryono Yadi Setiadi Yadi Setiadi Yadi Setiadi Yan Eka Prasetyawati YENNI BAKHTIAR YENNI BAKHTIAR YENNI BAKHTIAR Zainal Muttaqin Zuhriansah, Alfi Laila