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Deteksi Gen nuc (nuclease) Bakteri Methicillin Resistant Staphylococcus aureus (MRSA) Pada Sampel Ulkus Diabetikum Metode PCR Lestari, Ari Fajar; Suliati, Suliati; Anggraini, Anita Dwi
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2133

Abstract

Diabetic ulcers represent a common chronic complication of diabetes mellitus (DM) and are frequently associated with infections caused by Staphylococcus aureus. Staphylococcus aureus possesses multiple virulence factors, including the nuc (nuclease) gene, which encodes a thermonuclease enzyme capable of degrading DNA and destroying phagocytes within the host. Methicillin-resistant Staphylococcus aureus (MRSA) is a strain of S. aureus exhibiting resistance to methicillin and other beta-lactam antibiotics. This study used conventional PCR Methode to detect the presence of the nuc (nuclease) gene in MRSA isolates obtained from patients with diabetic ulcers. A descriptive quantitative study with a cross-sectional design was conducted on 30 diabetic ulcer samples collected from Rumah Rawat Luka Surabaya between April and May 2025. Phenotypic identification and antibiotic susceptibility testing were performed, followed by molecular detection of the nuc (nuclease) gene using the conventional PCR method, generating an amplicon of 279 bp. The findings revealed that 19 of the 30 diabetic ulcer swab samples were phenotypically identified as Staphylococcus aureus. Antibiotic susceptibility testing indicated that 6 (31.6%) of these isolates were methicillin-resistant (MRSA). Among the 6 MRSA isolates, 3 (50%) tested positive for the nuc (nuclease) gene. This study emphasizes the application of molecular-based diagnostic methods such as PCR for the rapid detection of MRSA in diabetic ulcers, thereby supporting the selection of appropriate antibiotic therapy and improving the success of clinical management.
PENGARUH KUALITAS PRODUK DAN LAYANAN TERHADAP KEPUASAN PELANGGAN SERTA DAMPAKNYA PADA LOYALITAS PELANGGAN DI RESTORAN PIZZA HUT CABANG JATIWARINGIN Wahyu Murti; Wirda Nursanti; Suliati
Jurnal Manajemen FE-UB Vol. 11 No. 1 (2023): Jurnal Manajemen FE-UB
Publisher : Fakultas Ekonomi Universitas Borobudur

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Abstract

This study aims to examine and analyze the effect of product and service quality on customer satisfaction and its impact on customer loyalty. The data used in this study are primary data taken from the results of questionnaire responses by pizza hut customers at the Jatiwaringin branch, East Jakarta. The data processing method uses the path analysis method with the help of the SPSS version 21 analysis tool. The statistical test in this study uses the goodness of fit test. The results showed that product quality had a direct and significant positive effect directly or indirectly through customer satisfaction on customer loyalty, service had a positive and significant effect directly and indirectly through customer satisfaction on customer loyalty and customer satisfaction had a direct positive effect on customer loyalty.   Keywords: product quality, service, customer satisfaction, customer loyalty.
Detection of mecA Gene in Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Diabetic Ulcer Swab Samples Aliyya Noor Izzati; Suliati; Lully Hanni Endarini; Syamsul Arifin
International Journal of Advanced Health Science and Technology Vol. 6 No. 1 (2026): February
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v6i1.500

Abstract

Diabetic ulcers represent a serious complication of diabetes mellitus, often becoming chronic wounds prone to infection due to impaired immunity, poor vascularization, and persistent hyperglycemia. Among the most frequently identified pathogens in such wounds is Staphylococcus aureus, with methicillin-resistant S. aureus (MRSA) strains posing significant therapeutic challenges due to their resistance to β-lactam antibiotics. This resistance is typically mediated by the mecA gene, which encodes penicillin-binding protein 2a (PBP2a), a protein with reduced affinity for β-lactams. This study aimed to detect the presence of the mecA gene in MRSA strains isolated from diabetic ulcer swabs and to examine the relationship between phenotypic resistance and genotypic confirmation. A total of 30 swab samples were collected from patients with diabetic ulcers at the Diabetes Wound Care Clinic in Surabaya. Bacterial identification was performed using standard microbiological techniques, and cefoxitin disk diffusion was used to screen for MRSA phenotypically. Genomic DNA from cefoxitin-resistant isolates was subjected to conventional PCR using mecA-specific primers, with electrophoresis to detect the expected 304 bp DNA fragment. Of the 30 samples, 19 (63%) were confirmed to be S. aureus, and among them, 6 isolates (31.6%) were resistant to cefoxitin. However, none of these isolates were positive for the mecA gene by PCR, although the positive control showed the expected band. This discrepancy suggests the possibility of other resistance mechanisms beyond mecA, highlighting the importance of molecular methods in confirming MRSA. These findings reinforce the need for integrated phenotypic and genotypic diagnostics to ensure accurate detection and appropriate treatment of MRSA infections
Comparison Of Fixation Using NBF 10% and Honey 13% with Time Variation On Mice Heart Histology Preparations Using Hematoxylin Eosin Staining Amirah Putri Zerlina; Juliana Christyaningsih; Ratno Tri Utomo; Suliati Suliati
International Journal of Advanced Health Science and Technology Vol. 5 No. 4 (2025): August
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v5i4.515

Abstract

The fixation stage is the initial stage of making histopathology preparations whose role is to maintain the cell structure of the tissue so that it is like living tissue and prevent tissue damage, thus facilitating the process of macroscopic and microscopic analysis. The fixative solution that is often used for standard preservation in anatomical pathology laboratory examinations is Neutral Buffered Formalin (NBF) 10%, which has the advantage of a pH that is close to neutral and effective in fixing soft tissue for 12-24 hours. However, NBF 10% is carcinogenic and has high toxicity. There is a natural fixative solution, honey, which contains fructose and glucose and has an acidic pH that has antimicrobial, antioxidant and antiautolysis properties. In addition, honey has also been widely used as a preservative because it is easily available, affordable, and without toxicity effects. The purpose of this study was to determine the comparison of the quality of histology preparations of mice heart tissue fixed with 10% NBF and 13% honey with time variations. This research is a type of laboratory experimental research conducted in November 2024-April 2025. The sample used is the heart of healthy male mice. The number of sample preparations used was 27 sample preparations. Data analysis through scoring and statistically processed using non-parametric Kruskal-Walis test, if there is a difference followed by Mann-Whitney test. The results showed that fixation with 13% honey solution with a time variation of 24 hours has the same comparison of preparation quality results with 10% NBF solution for 24 hours. Based on the Mann-Whitney test, the result is 0.018 (p < 0.05) for 13% honey solution for 12 hours and 0.634 (p > 0.05) for 13% honey solution for 24 hours. It can be concluded that fixation with 13% honey solution for 24 hours is effective as an alternative fixation solution with natural ingredients that are safe in fixing tissues
SENSITIVITY TEST OF KLEBSIELLA PNEUMONIAE FROM SPUTUM OF SUSPECTED PNEUMONIA PATIENTS TO THE ANTIBIOTIC CIPROFLOXACIN Salis Irawati; Suliati; Retno Sasongkowati; Juliana Christianingsih
Integrative Perspectives of Social and Science Journal Vol. 3 No. 06 Juni (2026): Integrative Perspectives of Social and Science Journal
Publisher : PT Wahana Global Education

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Abstract

Pneumonia is a pulmonary infection that may result in moderate to severe sickness across all age groups. The predominant microorganisms responsible for pneumonia are Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus, Klebsiella pneumoniae, Legionella species, Pseudomonas aeruginosa, and Acinetobacter species. Klebsiella pneumoniae is the bacterium most often responsible for pneumonia relative to other bacteria. This research is to assess the susceptibility of Klebsiella pneumoniae isolated from the sputum of patients suspected of pneumonia to the medication Ciprofloxacin. Sputum samples were collected from patients suspected of pneumonia between May and June 2024 at the Syarifah Ambami Rato Ebu Bangkalan Madura District Hospital, with further analysis conducted at the Surabaya Public Health Laboratory Center. The used methodology is sputum cultivation using aerobic techniques. The study used was a descriptive survey, determined by simple random sampling of 30 samples. The test findings indicated that 1 out of 30 samples (3.3%) exhibited Klebsiella pneumoniae bacteria, generating an inhibition zone of 32 mm, classified as sensitive according to CLSI recommendations. This study recommends that future studies use Klebsiella pneumoniae isolate samples from the sputum of probable pneumonia patients aged 2 to 64 years.
HUBUNGAN KADAR TROPONIN I DENGAN NILAI MEAN PLATELET VOLUME (MPV) DAN NILAI PLATELET LARGE CELL RATIO (P-LCR) PADA PASIEN SINDROM KORONER AKUT Kanaya Aulia Masardi; Evy Diah Woelansari; Wisnu Istanto; Suliati Suliati
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 5 No. 3 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v5i3.13590

Abstract

ABSTRACT Acute coronary syndrome (ACS) is a cardiovascular emergency caused by a sudden reduction in myocardial blood flow due to coronary artery obstruction, resulting in myocardial injury. Cardiac Troponin I is a specific biomarker of myocardial damage, whereas Mean Platelet Volume (MPV) and Platelet Large Cell Ratio (P-LCR) are indicators of platelet activity associated with atherothrombotic processes. This study aimed to analyze the relationship between Troponin I levels and MPV and P-LCR values in patients with ACS at Haji Regional General Hospital, East Java Province. An analytical observational study with a cross-sectional design was conducted from January to February 2026. A total of 30 patients diagnosed with ACS were selected using purposive sampling. Troponin I levels were measured using the Fluorescence Immunoassay method, while MPV and P-LCR were assessed using fluorescence flow cytometry. Data were analyzed using Spearman correlation analysis. The results showed that the mean Troponin I level was 2.49 ng/mL, the mean MPV was 10.29 fL, and the mean P-LCR was 26.63%. Spearman analysis demonstrated a significant positive correlation between Troponin I and MPV (p=0.047; r=0.365) as well as between Troponin I and P-LCR (p=0.039; r=0.380). It can be concluded that increased Troponin I levels are associated with elevated platelet activity parameters, indicating that MPV and P-LCR may serve as supportive hematological markers in the evaluation of patients with acute coronary syndrome. ABSTRAK Sindrom koroner akut (SKA) merupakan kegawatdaruratan kardiovaskular yang disebabkan oleh penurunan aliran darah ke miokard akibat penyumbatan arteri koroner dan berpotensi menimbulkan kerusakan jaringan jantung. Troponin I dikenal sebagai biomarker spesifik cedera miokard, sedangkan Mean Platelet Volume (MPV) dan Platelet Large Cell Ratio (P-LCR) merupakan indikator aktivitas trombosit yang berperan dalam proses aterotrombosis. Penelitian ini bertujuan menganalisis hubungan kadar Troponin I dengan nilai MPV dan P-LCR pada pasien SKA di RSUD Haji Provinsi Jawa Timur. Penelitian menggunakan desain observasional analitik dengan pendekatan cross-sectional yang dilaksanakan pada Januari–Februari 2026. Sebanyak 30 pasien SKA dipilih menggunakan teknik purposive sampling. Pemeriksaan Troponin I dilakukan dengan metode Fluorescence Immunoassay, sedangkan MPV dan P-LCR diperiksa menggunakan metode fluorescence flow cytometry. Analisis hubungan antarvariabel dilakukan menggunakan uji korelasi Spearman. Hasil penelitian menunjukkan rata-rata kadar Troponin I sebesar 2,49 ng/mL, MPV sebesar 10,29 fL, dan P-LCR sebesar 26,63%. Uji Spearman menunjukkan terdapat hubungan positif yang bermakna antara kadar Troponin I dengan MPV (p=0,047; r=0,365) serta antara kadar Troponin I dengan P-LCR (p=0,039; r=0,380). Disimpulkan bahwa peningkatan kadar Troponin I berkaitan dengan peningkatan parameter aktivitas trombosit, sehingga MPV dan P-LCR berpotensi menjadi parameter hematologi pendukung dalam evaluasi kondisi pasien sindrom koroner akut.
DRUG MANAGEMENT STRATEGIES IN DISASTER SITUATIONS Nuri Handayani; Imat Rochimat; Tovani Sri; Lully Hanni Endarini; Suliati Suliati
Media Penelitian dan Pengembangan Kesehatan Vol. 35 No. 4 (2025): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v35i4.3152

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Bencana merupakan rangkaian peristiwa yang mengancam dan mengganggu kehidupan dan penghidupan masyarakat. Bencana dapat memberi dampak pada sector kesehatan, infrastruktur dan ekonomi. Penelitian ini bertujuan untuk mengeksplorasi pengelolaan obat pada situasi kebencanaan di Indonesia, mengidentifikasi hambatan serta memberikan rekomendasi strategis. Penelitian ini menggunakan studi deskriptif kualitatif. Populasi dari penelitian ini yaitu tenaga farmasi yang terlibat langsung melakukan pekerjaan kefarmasian selama bencana. Data dikumpulkan dari 10 informan yang terdiri atas tenaga farmasi Dinas Kesehatan Kota/Kabupaten dan anggota Apoteker Tanggap Bencana (ATB) di daerah terdampak bencana. Data dikumpulkan melalui wawancara mendalam. Data yang diperoleh dianalisis menggunakan teknik analisis tematik dengan menggunakan model Miles dan Huberman. Hasil penelitian ini menunjukkan bahwa pengelolaan obat dalam situasi bencana di Indonesia belum berjalan optimal. Untuk mengatasi hal tersebut dibutuhkan koordinasi lintas sektor dan sistem pencatatan terintegrasi untuk memastikan ketersediaan obat yang efektif dan tepat sasaran. Rekomendasi yang dihasilkan meliputi penerapan SOP khusus, sistem pengadaan obat satu pintu dan program edukasi untuk pengungsi mengenai cara cerdas menggunakan obat terutama saat situasi bencana. Hasil penelitian ini dapat menjadi panduan untuk perbaikan manajemen pengelolaan obat di situasi darurat dan mendukung pengembangan kebijakan pengelolaan perbekalan farmasi dalam penanganan bencana.
Comparation Between Mac conkey and Coconut Water Medium as a Growth Medium for Escherichia coli Endah Prayekti; Suliati Suliati; Dwi Agustin Wulandari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1906

Abstract

Escherichia coli is the bacteria that can cause diarrhea in humans and often used as a parameter of stool environmental pollution. Culture of E. coli from the sample often requires Mac Conkey as commercial media which is able to distinguish it from other bacteria in the Enterobacteriaceae group. Commercial media such as Mac Conkey certainly has a price that is quite expensive because of its ability as a growth medium for Enterobacteriaceae. Therefore, in the study tested natural ingredients that can be used for growth media, such as coconut water. The purpose of this study was to compare the ability of Mac Conkey media and coconut water to support the growth of E. coli. This research is an experimental study with a completely randomized design. The concentration of coconut water tested was 0%, 20%, 40%, 60%, 80%, and 100%. The results showed that at the concentration of coconut water 20% to 60% the number of E. coli colonies on coconut water media was slightly below the Mac Conkey Agar media, while in coconut water a concentration of 80% showed a greater number of colonies than Mac Conkey. The Mann Whitney test showed a significant difference between the number of colonies on 80% coconut water media and Mac Conkey Agar, which was equal to 0.004 (p < 0.05). Based on these results, coconut water has the potential to be used as a growth medium for E. coli.
Health Education for Elementary School Age Children in the Kenosis Community, Surabaya Museyaroh; Juliana Christyaningsih; Yuni Ginarsih; Kasiati; Mujayanto; Nuning Marina Pengge; Kiaonarni O.W; Teresia Retna P; Pratiwi Hermiyanti; Rahayu Sumaningsih; Lembunai Tat Alberta; Evi Yunita Nugrahini; Yohanes Kambaru W; Teta puji Rahayu; Nurwening TW; Hery Sumasto; Suliati; Lully Hani Endarini; Evy Diah Woelansari; Ira Puspita Sari; Ira Rahayu Tiyar
Frontiers in Community Service and Empowerment Vol. 5 No. 1 (2026): March
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v4i2.96

Abstract

ABSTRACT The tutoring center "Kenosis" in Sidoyoso, established in 2016 by GKA Gloria Pos Sidoyoso, provides free educational support for elementary school children. This program helps in the academic and character development of children, initially taught by instructors from the Inter-University Christian Fellowship (Perkantas) and now reinforced by 4 permanent teachers. In addition to the need for tutoring, children are faced with daily conditions such as unhealthy snacks, low understanding of dental hygiene, and the importance of handwashing. These bad habits increase the risk of dental caries, infectious diseases, and the consumption of harmful food additives. Education and supervision are key to improving children's health and well-being. The Poltekkes Kemenkes Surabaya service team, consisting of 21 lecturers and 11 students, provided solutions to the above issues to 35 elementary school children at the Kenosis tutoring center on January 9, 2025, by delivering educational materials and practicing how to choose healthy snacks, brush teeth, and wash hands properly and correctly. Knowledge of harmful food additives The average pre-test score was 60.2, while the post-test score was 70.5. Understanding of proper tooth brushing techniques pre-test result 60.0 and post-test 70.5, understanding of good and correct handwashing habits had an average pre-test score of 60.0 and a post-test score of 70.5. There was an increase in knowledge about harmful food additives, proper tooth brushing techniques, and proper handwashing at the "Kenosis GKA Gloria Pos Sidoyoso" tutoring session. INDEX TERMS GKA Gloria, preservatives, food, PHBS, teeth.
Uji Efektivitas Daya Hambat Ekstrak dan Perasan Lidah Buaya (Aloe barbadensis Miller) pada Bakteri Staphylococcus Aureus dengan Metode Difusi Farah Adilah Ambar Sari; Suliati Suliati; Syamsul Arifin; Dwi Krihariyani
Termometer: Jurnal Ilmiah Ilmu Kesehatan dan Kedokteran Vol. 2 No. 4 (2024): Oktober : Termometer: Jurnal Ilmiah Ilmu Kesehatan dan Kedokteran
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/termometer.v2i4.4307

Abstract

Aloe vera is a plant that is rich in minerals, vitamins, and antibacterial properties. A study to analyze the antibacterial effectiveness of aloe vera in inhibiting the growth of Staphylococcus aureus bacteria isolated from acne pus. This study was an experimental laboratory study using the disc diffusion method, located at the Bacteriology Laboratory, Department of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya, from February to May 2024. There were 6 sample treatments with 4 replications, 4 treatments of extract and juice concentration variations, namely concentrations of 70%, 80%, 90%, 100%, positive control using penicillin antibiotics, and negative control using clean distilled water. The results of this study show that at aloe vera extract concentrations of 70%, 80%, 90%, and 100%, the average diameter of the inhibition zone was 9 mm, 10 mm, 11 mm, and 12 mm, respectively, while in aloe vera juice, these concentrations did not form an inhibition zone. So it was concluded that aloe vera extract is more effective than aloe vera juice, the most successful test material to slow the growth of Staphylococcus aureus bacteria in this study was aloe vera extract with a concentration of 100%. This study also conducted qualitative phytochemical testing on aloe vera extract which has antibacterial compounds, namely saponins, tannins, flavonoids, and terpenoids. The results of the study on the effectiveness of the inhibitory power of aloe vera extract and juice against Staphylococcus aureus bacteria using the disc diffusion method were analyzed using quantitative descriptive and presented in tabulation form in the form of the diameter of the inhibition zone formed.
Co-Authors ., Pestariati A.A. Ketut Agung Cahyawan W Aliyya Noor Izzati Amirah Putri Zerlina Anggraini, Anita Dwi Astuti Setiyani, Astuti Ayu Puspitasari Ayu Puspitasari, Ayu Bambang Hadi Sugito Briansky, Sabira Alvayogi Cholidah, Ema Zahiroh Nur Darjati Deddy Adam Dedi Kurniawan Della Ika Putri Damayanti Demes Nurmayanti Diah Titik Mutiarawati Diah Titik Mutiarawati Dwi Agustin Wulandari Endah Astuti, Sri Sulami Endarini, Lully Hani Endarini, Lully Hanni Endarini, Lully Hanny Era Fitria Yunita Ersanto, Novi Evi Yunita N Evi Yunita Nugrahini Evy Diah Woelansari Farah Adilah Ambar Sari Ginarsih, Yuni Hadi Suryono Handayati, Anik Haqqi, Faishol Hariyanto Hariyanto Hermiyanti, Pratiwi Hindaryani, Nurul Ilma Ilma Imat Rochimat Indah Lestari, Indah Ira Puspita Sari Ira Puspita Sari Ira Rahayu Tiyar Isfentiani, Dina Isnaini, Dian Istanto, Wisnu Juliana Christianingsih Juliana Christyaningsih Junaedi, Winda Rachmawati Kanaya Aulia Masardi Kartika Rahayuningsih, Christ Kasiati, Klanting Khambali, Khambali Kiaonarn Ongko Waluyo Kiaonarni O.W Kiaonarni OW Krihariyani, Dwi Kristiningsih, Elly Kuswanto, Muhammad Lembunai Tat Alberta Lembunai Tat Alberta, Lembunai Tat Lestari, Ari Fajar Liliek Soetjiatie Lully Hani Endarini Lully Hani Endarini Luthfi Rusyadi M. Zakarahman Makruf, Anang Mamik Mamik Mamik Mamik Maudita, Nauril MINARTI Minarti Minarti Mita Zuliana, Nur Mujayanto Museyaroh, Museyaroh Mutiarawati, Diah Titik N. S. Widodo Nadeak, Juan Carlos Nella Febrilia DP Novita, Devy Nuning Marina Pengge Nur Hatijah Nuri Handayani Nurwening TW Paramitha, Silvia Mega Prayekti, Endah Prisma Anjarlena Puspita Dewi, Teresia Retna Puspita, Ria Puspitasari, Erlita Rahayu Sumaningsih Rahayu, Teta Puji Ratno Tri Utomo Reni Susanti Rina Idawati Rusmania, Liya Safrudin, Samsul Saktiyani, Fisari Salis Irawati Salsyabillah Ramadhani HD. Widya Sari, Sintia Mustika Sasongkowati, Retno Siagian, Hotmaida Siti Alfiah Sri Utami Sugihani Suhariyadi Sumasto, Hery Syamsul Arifin Syamsul, Arifin Teresia Retna P Titin Muslihatin, Titin Toni Watoyani Tovani Sri Trisnania, Iid Dani Wahyu Murti Widyanur Widyawati, Keri Ayu Wirda Nursanti Yohanes Kambaru W Yusianti Silviani