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Sunarmi
Universitas Negeri Malang
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Journal : HAYATI Journal of Biosciences

 1 
Analysis of Genetic Potential of Banyuwangi Local Rice (Oryza sativa L.) Based on Relative Expression of Homogentisate Geranylgeranyl Transferase (HGGT) and Granule-Bound Starch Synthase I (GBSSI) Gene Dyati Galuh Pratita; Tita Putri Milasari; Elhah Nailul Khasna; Sunarmi Sunarmi; Evi Susanti; Suharti Suharti; Delia Wahyu Pangesti; Annasa Sabatia; Dwi Listyorini
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.181

Abstract

Rice nutrition including vitamin and amylose contents become important aspect for many people around the world. Rice with high amylose content (low glycemic index) is good for those with Diabetes mellitus. Tocotrienol, one precursor of Vitamin-E biosynthesis is catalyzed by enzymes encoded HGGT, while amylose biosynthesis is catalyzed by enzymes encoded GBSSI. The objective of this study was to find rice varieties with high tocotrienol and/or amylose content based on the expression of HGGT and GBSSI among eight Banyuwangi local rice varieties. Relative expression of HGGT and GBSSI was measured by qRT-PCR and analyzed using 2ΔCt method. Statistical analysis resulted in the significantly different of HGGT and GBSSI relative expression among samples. Relative expression of HGGT from the highest to the lowest were demonstrated by Hitam Melik, Hitam Pekat, Blambangan A3, Merah Bali, Blambangan A2, Berlian, Janur Kuning, and SOJ A3, respectively; while relative expression of GBSSI from the highest to the lowest were demonstrated by Hitam Melik, Hitam Pekat, SOJ A3, Janur Kuning, Berlian, Merah Bali, Blambangan A3, and Blambangan A2, respectively. Based on this research we conclude that Hitam Melik potentially produces higher tocotrienol and lower glycemic index than other studied varieties.
Co-Authors Ag. Kirwanto Agnes Lorentina Br Sembiring Ahmad Kamal Sudrajat Alessandro Golfried Andi Mulyono Anita Widiastuti Annasa Sabatia Annisa Annisa Rizki APRI KUNTARININGSIH Aprilli Dayanti Asnawi Hidayat Azizah Nur Rochmah Bagus Kusuma Ardi, Bagus Kusuma Bambang Guritno Bayu Indah Syafinatu Zafi Bening Tri Suwasono Bismar Nasution BUDIMAN GINTING Cheesya Siska Adhiati Christopher Gustikho Delia Wahyu Pangesti Deta Sukarja Devi Nirmala Muthia Sayekti DIAN LESTARI Dian Meinar Dian Natasia Dimas Fattih Dini Permata Sari Dwi Listyorini Dyati Galuh Pratita Eko Sri Sulasmi Elhah Nailul Khasna Elyas Franklin Erick Erick Erni - Widajanti Evelyn Evelyn Evi Susanti Ezra Cyntia Febrian Rosadi Fuadi, Fauzan Gunawan Sembiring HASIM PURBA Ikhsan Hidayat Ikhsan Lubis Jaelani Jaelani Jesica Pasaribu Junita Junita Keizerina Devi Khoirin Khoirin Kristania Felita Kurniawaty Kurniawaty Kwartaria Gultom Latief Abdul Maajid M. Ekaputra - Madiasa Ablisar Mahmul Siregar Malik Hamid Maruly Agustinus Maulana Ibrahim Mawaddah, K. Mega Riana Melati Fitri Melissa Simanjuntak Mellisa Tandoko Merinda Oktaviana michael Nasution Murni Murni Sapta Sari Neng Annis Fathia Nikita Rizky NINGRUM NATASYA SIRAIT Nopianti Nopianti Nurdin, I Qhairul Manurung Rafly Timothy Rahmat Hasibuan Rezki Arafah Rido Sigit Wicaksono Rifka Dameyanti Riris Fatmawati Robert Robert Rumata Rosininta Sianya S Sugiman, S Sari, M. S. Sarwono Selly Puspita Sari Shafira Mayada Shindih Hersiva Siti Hidayah Siti Kholifah Sri Rosa Steven Simanjuntak Suharti Suherwin Suherwin Sukarja, Detania Sulisetijono Sulisetijono Suprayitno Suprayitno Supriyadi, Andhi Susanti Delina Susilo Yulianto Sutiarnoto - Sylvia Vietressia Sinuhaji Syriac Nellikunnel Devasia Tahi Sitorus Tarsisius Murwadji Taufan Akbar Rifky Theofeni Yudea Tita Putri Milasari Tri Lubis Tri Murti Upsa Vision Wahyudi Ikhwal Wicaksono, Rido Sigit Widya Arisandy Wigrha Tommy Wiwin Renny Rahmawati