Article Per Year (5 Year)
p-Index From 2021 - 2026
2.884
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Media Veteriner Jurnal Sain Veteriner Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu Ternak Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) ZOO INDONESIA Jurnal Matematika Sains dan Teknologi Jurnal Perikanan Universitas Gadjah Mada IJEMS (Indonesian Journal of Environmental Management and Sustainability) Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Jurnal Riset Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Zoo Indonesia Jurnal Airaha Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Kefarmasian Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Iman Supriatna
Bagian Reproduksi Dan Kebidanan Departemen Klinik Reproduksi Dan Patologi Fakultas Kedokteran Hewan Institut Pertanian Bogor, Bogor
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Public Health Social Sciences Veterinary Other

Published : 59 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search
Journal : HAYATI Journal of Biosciences

 1 
In Vitro Fertilization and Embryo Development ITA DJUWITA; ARIEF BOEDIONO; SRIHADI AGUNGPRIYONO; IMAN SUPRIATNA
HAYATI Journal of Biosciences Vol. 12 No. 2 (2005): June 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.772 KB) | DOI: 10.4308/hjb.12.2.73

Abstract

Experiments were conducted on the morphology, fertilization and embryo development rate of vitrified ovine oocytes matured in vitro. Three vitrification solutions were used for vitrification. PBS supplemented with 1% BSA, 30% ethylene glycol was added by one of three different sucrose concentrations, 1.00 M (VS1), 0.50 M (VS2), and 0.25 M (VS3). The results showed that the percentages of normal vitrified oocytes after warming were 78 and 63% in VS1 and VS2, respectively, which was significantly higher as compared for VS3. The fertilization rates were 59 and 66% in VS1 and VS2, respectively, which were also significantly higher as compared with VS3 (35%). Zygote viability after 18 h was 57; 43; and 40%, for VS1,VS2, and VS3, respectively, which was not significantly different. The incidence of polyspermic penetration increased with increasing sucrose concentration, i.e 23, 11, and 9% in VS1, VS2, and VS3, respectively, as compared with unvitrified oocytes (4%). The cleavage rate of vitrified oocytes in VS1 was 13.2% which was significantly lower (p
Assessment of Fertility Status in the Male Sumatran Rhino at the Sumateran Rhino Sanctuary, Way Kambas National Park, Lampung MUHAMMAD AGIL; IMAN SUPRIATNA; BAMBANG PURWANTARA; DEDI CANDRA
HAYATI Journal of Biosciences Vol. 15 No. 1 (2008): March 2008
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.983 KB) | DOI: 10.4308/hjb.15.1.39

Abstract

Sumatran rhino is the most endangered rhino species. Its population is estimated less than 300 individuals remaining in the wild with highly declining rate to 50% in the last 15 years. The number of male rhinoceroses in the captivity are very few, therefore the assessment of its fertility is very important in order to support the breeding success since the captive breeding success is very poor. The objectives of this study were (i) to determine the male reproductive status, (ii) to establish a reliable semen collection method, and (iii) to assess semen parameters of the fresh collected sample. Three methods of semen collection were examined to determine its fertilizing potential, i.e. (i) stimulated combination of artificial vagina (AV), penile massage (PM) and accessory gland massage (AGM); (ii) AV and PM; and (iii) only with PM. The first method gave the best result with an ejaculation success of 85.71% (6/7, n = 7). The second and third methods obtained an ejaculation success rate of 50% (2/4, n = 4)) and 25% (1/4, n = 4), respectively. The collected ejaculates had a volume of 1.2-12.4 ml with whitish to cream turbid colour and pH 6.90-6.99. Sperm concentration was (143-333) x 103 sperm/ml. The quality of the sperm was low with only approximately 1% of them moved forward slowly. Approximately 80% of the spermatozoa were immature (prox. cytoplasmic droplet) with head (macro-, microcephalic) and tail abnormalities (broken tail). Semen quality increased after several collections and the amount of immature sperm decreased up to 5%. Electroejaculation procedure could produce 34 ml semen, but no sperm was found in the ejaculate. Hence, the combination of AV, PM, and AGM could get higher volume of ejaculate compared to other methods, but sperm concentration was better obtained using AV and PM only. Repeated semen collection increased semen quality, although the male has low fertilizing capacity due to low sperm concentration (oligozoospermia) and small volume of the ejaculate (oligospermia). Key words: Sumatran rhino, semen, sperms, collection
Heterotopic grafting sites can be useful in producing oocytes for in vitro Fertilization, therefore, maximising the oocyte yield from the graft by gonadotrophin stimulation would be advantageous. The aim of this study was to investigate the number and quality of oocytes collected from heterotopic autografted ovary after Pregnant Mare Serum Gonadothropin (PMSG) induction. Graft recipients were treated either with or without PMSG stimulation 48 hours prior to graft collection. Ovarian tissue from . NURBARIAH; ITA DJUWITA; KUSDIANTORO MOHAMAD; IMAN SUPRIATNA
HAYATI Journal of Biosciences Vol. 18 No. 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.4.151

Abstract

Heterotopic grafting sites can be useful in producing oocytes for in vitro Fertilization, therefore, maximising the oocyte yield from the graft by gonadotrophin stimulation would be advantageous. The aim of this study was to investigate the number and quality of oocytes collected from heterotopic autografted ovary after Pregnant Mare Serum Gonadothropin (PMSG) induction. Graft recipients were treated either with or without PMSG stimulation 48 hours prior to graft collection. Ovarian tissue from four weeks old mice (DDY strain) were autotransplanted under the kidney capsule of the same ovariectomized mice and the oocytes were collected 21 days after autotransplantation. The results showed that the average number of oocytes collected from autografted ovaries without PMSG induction were 9.0. + 2.8 not significantly different with those received PMSG induction, 10.9 + 5.1. The percentage of matured and fertilized oocytes and the developed embryos from the autografted ovaries without PMSG induction were 52.4, 33.4, and 26.0%, respectively not significantly different with those received PMSG induction, 53.2, 35.1, and 29.9%, respectively. The number of oocytes and the capacity to matured, fertilized and developed were significantly lower (P < 0.05) compared to the superovulated nongrafted (control) ovaries. In conclusion, PMSG induction on the graft recipients did not significantly increase oocytes yield from grafted heterotopic ovaries. The number and quality of oocytes produced from the autografted ovaries were lower than the superovulated nongrafted ovaries, but still can be used for in vitro embryo production after sequential in vitro maturation and fertilization.
Co-Authors . Hasbi . NURBARIAH A Amrozi Abadi, Agung S. Adi Winarto ahmad yani Ahmad Yani Amrozi Amrozi, A Antje Engelhardt Antje Engelhardt Antje Engelhardt, Antje Aras Prasetiyo Nugroho Arief Boediono Asma, Siti Asri Pudjirahaju Bambang Purwantara Benny Heltonika Cece Sumantri Cutnya’ Shaliran Nazlie (Alm) DEDI CANDRA Dedi Rahmat Setiadi Dondin Sajuthi Ekayanti Mulyawati Kaiin Endang - Gunaisah Eva Handayani Gholib Gholib Gholib Gholib, Gholib Gono Semiadi GONO SEMIADI Gozali Moekti Gustina, Sri Hadi Nurohman Hamid Hamid Hamid Hamid Hasbi . Heistermann, Michael Hismayasari, Intannurfemi Hismayasari, Intannurfemi B. Hismayasari, Intanurfemi B. Hurip Pratomo Hurip Pratomo I Gusti Ayu Budiadnyani I Gusti Wayan Murjana Yasa Intannurfemi B. Hismayasari Intannurfemi Hismayasari Ismail Ismail ITA DJUWITA Ita Djuwita Juli Melia Juli Melia Juli Melia Kresno Suharto Kusdiantoro Mohamad La Ode Syawal Sulaeman Lies Parede Hernomoadi M Agus Setiadi Michael Heistermann Mitha Kurnia Sari Moh. Sayuti Mohamad Fahrudin MUHAMMAD AGIL Muhammad Imron Muhammad Imron Muhammad Imron Muhsuryono Muhsuryono Muhsuryono, Muhsuryono Musthamin Balumbi Nazlie (Alm), Cutnya’ Shaliran Ni Wayan Kurniani Karja Nofri Zayani Nurbety Tarigan Nurohman, Hadi Pardede, Berlin Pandapotan Prajayanti, Vini Taru F. R. Iis Arifiantini R. Rusli R. Taufiq Purna Nugraha R. Taufiq Purna Nugraha Rahman, Ainul Alim Rahmatullah Rahmatullah Rahmatullah Rahmatullah Ridwan Affandi RIDWAN AFFANDI Rimas Prathita Agustin Rudy Priyanto Saidin Saidin Saidin, Saidin saidin, saidin Sari, Mitha Kurnia Sayuti, Moh. Sayuti, Mohammad Setiyono, Achmad Siska Adelya Ramadhani Soni Sopiyana Sri Gustina Srihadi Agungpriyono Surya Kusuma Wijaya Suwandi, Syifa Damaianti Syamsiar, Syamsiar Tambajong, Daisy TATI NURHAYATI Taufiq P. Nugraha Teguh Sumarsono Thomas Mata Hine Tuty Laswardi Yusuf Vini Taru Febriani Prajayati Wasmen Manalu Yundari, Yundari Yusmala, Mitha