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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Media Veteriner Jurnal Sain Veteriner Jurnal Veteriner Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu Ternak Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) ZOO INDONESIA Jurnal Matematika Sains dan Teknologi Jurnal Perikanan Universitas Gadjah Mada Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Jurnal Riset Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Zoo Indonesia Jurnal Airaha Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Kefarmasian Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Iman Supriatna
Bagian Reproduksi Dan Kebidanan Departemen Klinik Reproduksi Dan Patologi Fakultas Kedokteran Hewan Institut Pertanian Bogor, Bogor
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Journal : Media Veteriner

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Study on the Application of Human Chorionic Gonadotropin (hCG) in Dairy Cow Superovulated with Pregnant Mare Serum Gonadotropin - Monoclonal Antibody (PMSG-MoAb) Anti PMSG Iman Supriatna; Tuty Laswardi Yusuf; Bambang Purwantara; Gozali Moekti; Lies Parede Hernomoadi
Media Veteriner Vol. 5 No. 2 (1998): Media Veteriner
Publisher : Media Veteriner

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Abstract

The research was planned to yield the biologic potential synergism of MoAb-hCG in increasing the embryo number. Twenty five dairy cows were divided into five groups. The Control Group was superovulated with 2,500 IU PMSG intramuscularly (i.m.), the remaining of the Group II, III, IV and V consecutively after being superovulated by 2,500 IU PMSG were treated with MoAb intravenously (i.v.), hCG i.v., MoAb-hCG i.v. and MoAb i.v-hCG i.m. Data evaluation were analyzed using ANOVA and LSD test. The result showed that there were increasing in number of transferable embryo per donor produced (P<0.01) after application with either MoAb i.v. (9.6), hCG i.v. (9.4), MoAb-hCG i.v. (11.2), or MoAb i.v.-hCG i.m. (9.6). The Control Group produced only 2.4 embryo per donor. The combined MoAb and hCG in one treatment were not able to increase the embryo yield (P>0.05).Key words: MoAb, PMSG, hCG, superovulation, donor, embryo
The Use of Direct Transfer Method on Embryo Cryopreservation in Dairy Cattle Iman Supriatna; Tuty Laswardi yusuf; Bambang Purwantara; Gozali Moekti; Lies Parede Hernomoadi
Media Veteriner Vol. 6 No. 1 (1999): Media Veteriner
Publisher : Media Veteriner

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Abstract

The experiment was carried out to study the use of direct transfer method in embryo cryopreservation by using two cryoprotectants and the effectivity of various concentrations of sucrose during cryoprotectant removal. Eighty-fourmorula stage embryos were divided equally into two groups and were treated by using 1.5 M ethylene glycol (EG) and 1.5 M 1,2-propanediol as cryoprotectant. The embryos were frozen using programmable embryo freezing machine on step by step decreasing temperature. Frozen embryos were thawed and cryoprotectants were removed either without sucrose (0 M) or with sucrose in concentration of 0.2 M, 0.4 M and 0,8 M. The results showed that the quality of the thawed embryos cryopreserved using 1.5 M EG was better than that using 1.5 M PROH. The survival rate on the embryos cryopreserved with 1.5 M EG (92.8%) was higher than 1.5 M PROH (78.6%) (P0.05). The viability of embryos exposed to 0 M, 0.2 M, 0.4 M and 0.5 M sucrose were 80.0, 80.8, 90.9 and 81.80% respectively. In contrast, by using 1.5 M PROH, rehydration with 0.4 M (83.3%) and 0,8 M (90.0%) sucrose was significantly better compared to those without (22.2%) or with 0.2 M (36.3%) sucrose (p
Co-Authors . Hasbi . NURBARIAH A Amrozi Abadi, Agung S. Adi Winarto ahmad yani Ahmad Yani Amrozi Amrozi, A Antje Engelhardt Antje Engelhardt Antje Engelhardt, Antje Aras Prasetiyo Nugroho Arief Boediono Asma, Siti Asri Pudjirahaju Bambang Purwantara Benny Heltonika Cece Sumantri Cutnya’ Shaliran Nazlie (Alm) DEDI CANDRA Dedi Rahmat Setiadi Dondin Sajuthi Ekayanti Mulyawati Kaiin Endang - Gunaisah Eva Handayani Gholib Gholib Gholib Gholib, Gholib GONO SEMIADI Gono Semiadi Gozali Moekti Gustina, Sri Hadi Nurohman Hamid Hamid Hamid Hamid Hasbi . Heistermann, Michael Hismayasari, Intannurfemi Hismayasari, Intannurfemi B. Hismayasari, Intanurfemi B. Hurip Pratomo Hurip Pratomo I Gusti Ayu Budiadnyani I Gusti Wayan Murjana Yasa Intannurfemi B. Hismayasari Intannurfemi Hismayasari Ismail Ismail ITA DJUWITA Ita Djuwita Juli Melia Juli Melia Juli Melia Kresno Suharto Kusdiantoro Mohamad La Ode Syawal Sulaeman Lies Parede Hernomoadi M Agus Setiadi Michael Heistermann Mitha Kurnia Sari Moh. Sayuti Mohamad Fahrudin MUHAMMAD AGIL Muhammad Imron Muhammad Imron Muhammad Imron Muhsuryono Muhsuryono Muhsuryono, Muhsuryono Musthamin Balumbi Nazlie (Alm), Cutnya’ Shaliran Ni Wayan Kurniani Karja Nofri Zayani Nurbety Tarigan Nurohman, Hadi Pardede, Berlin Pandapotan Prajayanti, Vini Taru F. R. Iis Arifiantini R. Rusli R. Taufiq Purna Nugraha R. Taufiq Purna Nugraha Rahmatullah Rahmatullah Rahmatullah Rahmatullah Ridwan Affandi RIDWAN AFFANDI Rimas Prathita Agustin Rudy Priyanto Saidin Saidin saidin, saidin Saidin, Saidin Sari, Mitha Kurnia Sayuti, Moh. Sayuti, Mohammad Setiyono, Achmad Siska Adelya Ramadhani Soni Sopiyana Sri Gustina Srihadi Agungpriyono Surya Kusuma Wijaya Suwandi, Syifa Damaianti Syamsiar, Syamsiar Tambajong, Daisy TATI NURHAYATI Taufiq P. Nugraha Teguh Sumarsono Thomas Mata Hine Tuty Laswardi Yusuf Vini Taru Febriani Prajayati Wasmen Manalu Yundari, Yundari Yusmala, Mitha