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Anti-tumor agent celecoxib activity towards SP-C1 tongue cancer cells invasion (in vitro) Achmad, Harun; Satari, Mieke Hemiawati; Oewen, Roosje Rosita; Supriatno, S.
Padjadjaran Journal of Dentistry Vol 23, No 1 (2011): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1465.155 KB) | DOI: 10.24198/pjd.vol23no1.14053

Abstract

Invasion is a characteristic of the occurrence of cancer and indicates the cancer cells capability to destroy and degrade the border between the epithet and basal membrane to further spread into the surrounding extra-cellular matrix. The purpose of this research was to find the existence of impediment at the SP-C1 tongue cancer cell using celecoxib chemopreventive medication. The SP-C1 tongue cancer cells were treated in vitro using celecoxib medication as a research subject at the following concentrations 5, 10, 25, 50, 75, 100, 125%; and 0 as control group (only DMEM growth medium treatment). Pure experimental testing was carried out for 24 and 48 hours, with observation and calculation of an average number of SP-C1 tongue cancer cells. The data collected were analyzed using the ANOVA test with Newman Keuls paired range test or t-test. Research results indicated that the average number of SP-C1 tongue cancer cells invasion after administration of celecoxib medication based on administration concentration and time statistically yielded significant results. The ANOVA test results were statistically significant, that is, average occurrence of the number of SP-C1 tongue cancer cells due to the use of celecoxib at certain concentrations compared to that without celecoxib was different. At celecoxib of zero (control) concentration was 24.4 with celecoxib concentration starting at 5 up to 125% experienced a decline from its average 11 to become 2.3. The conclusion of the research was that the greater the celecoxib concentration administered, the greater the effect on the impediment of SP-C1 tongue cancer cell invasion.
Epidemiologic profile of oral squamous cell carcinoma in Yogyakarta, Indonesia Gracia, Isadora; Utoro, Totok; S., Supriatno; Astuti, Indwiani; Heriyanto, Didik Setyo; Pramono, Dibyo
Padjadjaran Journal of Dentistry Vol 29, No 1 (2017): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.633 KB) | DOI: 10.24198/pjd.vol29no1.11614

Abstract

ABSTRACTIntroduction: Oral cancer is a major health problem in the world, indicated by a high incidence of recurrence. In Southeast Asia, oral cancer ranks sixth from all cancers on the human body. The incidence of oral cancer, especially squamous cell carcinoma types, increases every year, but the prognosis and patients life expectancy are still unsatisfying enough and the reports about the profile of oral cancer is still limited.The objective of this research was to get the epidemiological profile of oral squamous cell carcinoma (OSCC) in Yogyakarta, Indonesia. Methods: The research method was observational analytic with cross-sectional design. All new OSCC cases in three anatomic pathology laboratory installations in Yogyakarta (January 2011-December 2015) were included in this study. Data collected were gender, age, anatomical location, and histology. All data were analyzed using statistical analysis program. Results: OSCC new cases amount increasing each year: 6 cases (6.6%) in 2011, and increased to 24 cases (26.4%) in 2015. Fifty-two of the 91 cases (57.1%) were found on male. The age group with the most cases was found in group of 41-60 years (49.5%). There is no difference in the case distribution by age between male and female. Forty-eight cases were found in the lingual part (52.7%). Histological examination showed 68 cases (74.7%) were well-differentiated carcinoma. Conclusion: OSCC profile in Yogyakarta mostly found in men with anatomical location in the lingual part in the age group above 40 years of age and were having well-differentiated histology.Keywords: Oral squamous cell carcinoma (OSCC), Epidemiologic profile, Yogyakarta 
ANTI TUMOR ACTIVITY OF PCDNA3.1-TUBEROUS a COMPLEX-2 SENSE IN A HUMAN ORAL TONGUE CANCER (SP-C1) CELL LINE: AKTIVITAS ANTI TUMOR PCDNA3.1-TUBEROUS SCLEROTIC COMPLEX-2 SENSE PADA SEL KANKER LIDAH MANUSIA (SP-C1) Supriatno
Dentika: Dental Journal Vol. 17 No. 1 (2012): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.655 KB) | DOI: 10.32734/dentika.v17i1.1747

Abstract

Tuberous sclerosis complex (TSC) is a hamartomatous disease with defects in tuberin (TSC2) that can be autosomaldominant inheritance or spontaneous mutation. Mutation of the TSC-2 gene encoding tuberin on chromosome 16p13.3increases to the clinical disorder of tuberous sclerosis characterized by the development of hamartomas. In the presentstudy, the antitumor activity of pcDNA3.1-TSC2 sense in an oral tongue cancer Supri’s clone-1 (SP-C1) wasinvestigated. An expression vector containing sense-oriented rat TSC-2 pcDNA with pcDNA3.1 and transfected to cell(SP-C1) to regulate the expression of TSC-2 gene in each transfectant was constructed. MTT assay was performed toexamine the SP-C1 cell growth suppression transfected by pcDNA3.1-TSC2. Caspase-3 and -9 were conducted toobserve the induction of cell apoptosis. Western blotting analysis was carried out to determine the protein level of TSC-2,p27Kip1 and -tubulin. The results showed that, overexpression of TSC-2 exerted the growth inhibitory effect of SP-C1cell and markedly increased apoptosis via caspase-3 and -9 pathways (P=0.001). Sense-oriented SP-C1-cDNA3.1-TSC-2cancer cells have a high expression of p27Kip1. In conclusion, pcDNA3.1-TSC-2 sense increased the antitumor activity oforal tongue cancer SP-C1 cell through p27Kip1 induction.
KARAKTERISTIK SEL KANKER ORAL BARU (Sp-C1) DAN UJI HAMBATAN PERTUMBUHAN SEL Sp-C1 MENGGUNAKAN TERAPI GEN pcDNA3.1 -p27Kip1mutant type in vitro: CHARACTERISTICS OF NEW ORAL CANCER CELL (Sp-C1) AND TEST OF Sp-C1 CELL GROWTH INHIBITION USING A pcDNA 3.1-p27Kip1mutant type gene in vitro Supriatno
Dentika: Dental Journal Vol. 18 No. 2 (2014): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.412 KB) | DOI: 10.32734/dentika.v18i2.2015

Abstract

Sel primer kanker oral (sel Sp-C1) mempunyai beberapa karakteristik pertumbuhan sel dan ekspresi protein antiapoptosis maupun protein regulator positif siklus sel. Karakteristik sel Sp-C1 penting diketahui untuk memudahkanpeneliti menggunakan model in vitro sel kanker oral. Tujuan penelitian adalah memperkenalkan jenis sel kanker oral baru(sel Sp-C1) dengan karakteristiknya, serta menguji hambatan pertumbuhan sel Sp-C1 mengunakan terapi gen p27Kip1mt secara in vitro. Isolasi sel Sp-C1 berasal dari jaringan limfonodi servikal yang termetastasis kanker lidah. Proses isolasididapatkan fibroblas dan sel primer kanker oral yang diinkubasi pada refrigerator -800C. Hambatan pertumbuhan sel SpC1 diperlakukan dengan terapi gen pcDNA3.1 -p27Kip1 mt dan pcDNA3.1-neo (empty vector) menggunakan uji MTT.Hasil penelitian menunjukkan karakteristik pertumbuhan sel Sp-C1 yang relatif cepat dan ekspresi protein p45Skp2, αtubulin, cyclin-dependent kinase-2 (CDK-2), cyclin-E, metastatic associated protein-1 (MTA-1) dan protein antiapoptosis maspin. Selanjutnya, sel transfektan Sp-C1-pcDNA3.1-p27Kip1 mt mempunyai potensi hambatanpertumbuhan sel yang signifikan dibandingkan pcDNA3.1-neo (P< 0.05). Kesimpulan, sel Sp-C1 mempunyaikarakteristik tertentu dan dapat digunakan sebagai model penelitian in vitro kanker mulut. pcDNA3.1-p27Kip1 mtmempunyai potensi hambatan pertumbuhan sel Sp-C1 yang kuat. Metode transfer gen secara in vitro merupakan proseduryang sederhana dan suatu strategi baru terapi gen terhadap sel kanker oral.
Efek Ekstrak Etanol Daun Keladi Tikus (Typhonium flagelliforme Lodd.) pada Invasi Sel Kanker Lidah Manusia (SP-C1) in vitro Zakiyana, Yulida; Supriatno, -; Medawati, Ana
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 10, No 2 (2010)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v10i2.1579

Abstract

The aim of study was to examine the anti invasion potency ethanolic extract of Typhonium flagelliforme Lodd. leaves toward the growth inhibition of human oral tongue cancer cell Supri ’s Clone-1 (SP-C1). The design of this study was pure laboratory experimental research with the sample of human oral tongue cancer cell (SP-C1). SP-C1 invasion was inhibitad by ethanolic extract of Typhonium flagelliforme Lodd. leaves in some concentrations (0, 25, 50, 75, 100, 125 pg/ml). The samples were then incubated in 24 hours. As a control SP-C1 was grown in Rosswell Park Memorial Institute 1640 (RPMI-1640). Boyden chamber kit were used in the study. Result of the study showed that ethanolic extract of Typhonium flagelliforme Lodd. leaves was markedly inhibit the invasion of SP-C1 cell. The concentration of 125 pg/ml was found ethanolic extract of Typhonium flagelliforme Lodd. leaves has more to inhibit potency the invasion of SP-C1 cell. The conclusion ethanolic extract of Typhonium flagelliforme Lodd. leaves has significant invasion to inhibit potency the invasion of human oral tongue cancer cell line (SP-C1).Penelitian ini bertujuan untuk mengkaji efek ekstrak etanol daun tanaman keladi tikus (Typhonium flagelliforme Lodd.) terhadap invasi sel kanker lidah manusia (SP-C1). Jenis penelitian ini adalah penelitian eksperimental laboratoris murni terhadap sel kanker lidah manusia (SP-C1) yang diberi perlakuan dengan ekstrak etanol daun tanaman keladi tikus. Biakan sel SP-C1 diinkubasikan dengan ekstrak etanol daun keladi tikus dalam berbagai konsentrasi (0, 25, 50, 75, 100, 125 pg/ml) selama 24 jam, sebagai kontrol negatif digunakan biakan sel SP-C1 dalam Rosswell Park Memorial Institute 1640 (RPMI-1640). Alat ukur yang digunakan untuk mengetahui aktivitas invasi sel SP-C1 setelah diberi perlakuan ekstrak etanol daun tanaman keladi tikus menggunakan alat Boyden chamber. Berdasarkan hasil penelitian menunjukkan penurunan jumlah biakan sel SP-C1 secara signifikan terlihat pada perlakuan dengan ekstrak etanol daun keladi tikus konsentrasi 125 pg/ml dibandingkan kontrol maupun kelompok perlakuan. Disimpulkan bahwa ekstrak etanol daun keladi tikus mempunyai pengaruh signifikan dalam menghambat invasi sel SP-C1.
Daya Hambat Ekstrak Etanol Aloe Vera L. terhadap Proliferasi Sel Kanker Rongga Mulut (Sp-C1) secara In Vitro Putri, Gina Arfianti; Supriatno, -; Medawati, Ana
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 12, No 1 (2012)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v12i1.996

Abstract

Kanker adalah penyakit yang ditandai dengan pembelahan sel yang tidak terkendali dan kemampuan sel-sel tersebut untuk menyerang jaringan biologis lainya, Pada penelitian ini menggunakan Lidah Buaya (Aloe vera L) yang memiliki  banyak khasiat sebagai anti kanker, anti bakteri, anti jamur, anti inflamasi, dan memiliki efek analgesik. Tujuan dari penelitian ini adalah mengkaji daya hambat proliferasi sel kanker rongga mulut (SP-C1) menggunakan  ekstrak etanol Lidah buaya. Desain penelitian ini adalah eksperimental laboratoris murni. Subjek penelitian pada penelitian ini menggunakan kultur sel kanker lidah (SP-C1) yang dibiakan dalam media Dubelcco’s modified eagle medium (DMEM) yang diberi foetal Bovine Serum 10% (FBS). JenisLidah buaya yang digunakan pada penelitian ini adalah Aloe vera L arborescens. Sel SP-C1 yang tumbuh sub-confluent dipanen menggunakan Tripsin-EDTA 0,25%. Sel sebanyak 1x 104 sel/sumur dimasukkkan cawan petri 24 sumur, sesuai jumlah dengan konsentrasi ekstrak etanol Lidah buaya yang digunakan. Sel di inkubasi selama 24 jam. Setelah inkubasi, semua media dibuang dan diganti dengan media baru yang mengandung berbagai konsentrasi ekstrak Lidah buaya. Sel di inkubasi selama 0, 24, 48 dan 72 jam. Hasil Penelitian menunjukkan pemberian ekstrak etanol Lidah buaya pada konsentrasi 75 mg/ml dan 100 ml/mg cenderung menurunkan jumlah proliferasi sel SP-C1 dibandingkan dengan konsentrasi 0 mg/ml, 25 mg/ml, dan 50 mg/ml dengan signifikansi p0.05. Kesimpulan: Ekstrak etanol Lidah buaya efektif menghambat proliferasi sel kanker rongga mulut SP-C1 secara In Vitro. Cancer is a desease that have characterized uncontrolled mitosis and invasion to the other organs. In this researched used Aloe vera as alternative herbal to cure cancer. Aloe vera contains are anticancer, antifungi, antiinflamation and analgesic. The aim of this research is to know the effect of etanolic extract of Aloe vera in inhihibit proliferation of SP-C1. Design research is pure laboratory experimental. Subject is a culture SP-C1 cells in Dubelcco’s modified eagle medium (DMEM) and foetal Bovine Serum 10% (FBS). The kind of Aloe vera is Aloe vera L arborescens. The Fluectuent SP-C1 cell collect by Tripsin-EDTA 0,25%. Cell put in cawan petri 24 cell/well with etanolic extract of Aloe vera, incubation 24  hours and replace with new media, incubation in 24, 48 and 72 hours. Each group add with MTT solution and counted by ELISA. The  result of this research shows that concentration 75 mg/ml  and 100 mg/ml effective inhibit proliferation of SP-C1 cell compared with 0 mg/ml, 25 mg/ml  and 50 mg/ml with the value of significant level 0.05.
Pelaksanaan good agricultural practices dan penerapan skema fair trade pada perkebunan kopi rakyat Jawa Tengah Supriatno
Jurnal Bisnis Kehutanan dan Lingkungan Vol. 1 No. 1: (July) 2023
Publisher : Institute for Advanced Science, Social, and Sustainable Future

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61511/jbkl.v1i1.2023.257

Abstract

The implementation of Good Agricultural Practices (GAP) on smallholder coffee plantations in Central Java is considered a solution that has the potential to increase coffee production while protecting biodiversity and achieving sustainable plantations. Several preliminary studies show that implementing GAP is directly proportional to the productivity of crops and prevents monoculture plantation methods, which are detrimental to the environment and productivity. Apart from that, another problem being addressed is the low welfare of coffee farmers in Central Java due to the low purchasing price of coffee and the dominant export in the form of raw coffee beans. This province can take steps by introducing a fair trade scheme for coffee farmers who are members of cooperatives or farmer groups. By implementing fair tradeprinciples, farmers' welfare can increase through equal welfare distribution between farmers and sellers, price transparency, new business opportunities, decent wages for workers, and environmentally friendly production and sales processes. Through analysis of the two problems above, this article aims to provide a deeper understanding of the importance of implementing GAP and fair trade schemes in increasing the productivity and welfare of coffee farmers in Central Java, as well as contributing to the development of sustainable and environmentally sound coffee plantations in Indonesia.
Assessing the quality of mangrove ecosystem as breeding sites organisms in the coastal area Ulva, Susi Mulia; Djufri, Djufri; Supriatno, Supriatno; Safrida, Safrida; Huda, Ismul
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 13, No 1 (2024): APRIL 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.1.31348

Abstract

Mangrove ecosystem is an ecosystem that supports people's lives and the biota that live in it. Globally, mangrove ecosystems are recognized as productive ecosystems in coastal areas. Mangrove wetlands have an important function of protecting coastal ecology, buffering salinity, breeding grounds for organisms and transporting nutrients. Research on the assessment of the quality of mangrove ecosystems in Syiah Kuala District, Banda Aceh City was carried out with the aim of studying the presence of mangrove species, plankton species, hydrological factors of mangrove ecosystems and analyzing the quality of mangrove ecosystems as breeding grounds for organisms based on hydrological factor values. The method used in this study was the line transect method and the research technique was carried out by means of purposive sampling. Data analysis was calculated using the Shanon-Wiener Species Diversity Index () and PCA (Principal Component Analysis) analysis. Identification results showed a low mangrove species diversity index with a value of = 1.924, a plankton species diversity index of = 2.614 belonging to moderate community stability, a hydrological index covering temperatures ranging from 28.2 to 29.8 C, pH 7.1 -7.4, DO 2.9-4.1 mg/L, salinity 27-20.9 /, brightness 30-68.5 Cd, depth 88.5-105 cm. Water quality parameters are in good condition. PCA analysis showed a correlation between the distribution of mangrove species and parameters of salinity, pH, DO, temperature, depth and brightness.Keywords:MangrovesBreeding SiteHydrologyPlanktonCoastal Area
Utilization of Radioactive Contaminated EAF Dust as Material for Making Paving Block Supriatno, Supriatno; Martono, Dwi Nowo; Agustina, Haruki
International Journal of Multidisciplinary: Applied Business and Education Research Vol. 4 No. 6 (2023): International Journal of Multidisciplinary: Applied Business and Education Rese
Publisher : Future Science / FSH-PH Publications

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/ijmaber.04.06.08

Abstract

Electric arc furnace dust, due to steel fabrication by-products, some of that material has been contaminated with radioactive materials from orphan sources. After calculating the concentration of radionuclide activity, it is known that the material meets the criteria for clearance of application and can be used for other purposes according to the appropriate characteristics. By determining the clearance, EAF dust will be processed through a stabilization/solidification method to be used as a material for making paving blocks. Through elemental analysis of EAF dust using the Micro-XRF method, it is known that sample 1 has a Ca content of 3.9%, CaO is 5.51%, Si is 0.64%, and SiO2 is 1.36%, while sample 2 has Ca content. 4.8%, CaO 6.74% Si 0.63% and SiO2 1.34%. The compressive strength test results based on Indonesian National Standard (SNI) 03-0691-1996 give the results of paving blocks included in quality D with a value of 9.2 MPa which can be used for parks or other purposes. In the calculation of cost savings analysis, determining the clearance for EAF dust can provide cost savings of Rp. 205,562,000 compared to if it had to be managed as radioactive waste. The production cost for one paving block using a mixture of EAF dust is Rp. 2,213.77 or Rp. 97,405.88 for units per m2.
Assessing the quality of mangrove ecosystem as breeding sites organisms in the coastal area Ulva, Susi Mulia; Djufri, Djufri; Supriatno, Supriatno; Safrida, Safrida; Huda, Ismul
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 13, No 1 (2024): APRIL 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.1.31348

Abstract

Mangrove ecosystem is an ecosystem that supports people's lives and the biota that live in it. Globally, mangrove ecosystems are recognized as productive ecosystems in coastal areas. Mangrove wetlands have an important function of protecting coastal ecology, buffering salinity, breeding grounds for organisms and transporting nutrients. Research on the assessment of the quality of mangrove ecosystems in Syiah Kuala District, Banda Aceh City was carried out with the aim of studying the presence of mangrove species, plankton species, hydrological factors of mangrove ecosystems and analyzing the quality of mangrove ecosystems as breeding grounds for organisms based on hydrological factor values. The method used in this study was the line transect method and the research technique was carried out by means of purposive sampling. Data analysis was calculated using the Shanon-Wiener Species Diversity Index () and PCA (Principal Component Analysis) analysis. Identification results showed a low mangrove species diversity index with a value of = 1.924, a plankton species diversity index of = 2.614 belonging to moderate community stability, a hydrological index covering temperatures ranging from 28.2 to 29.8 C, pH 7.1 -7.4, DO 2.9-4.1 mg/L, salinity 27-20.9 /, brightness 30-68.5 Cd, depth 88.5-105 cm. Water quality parameters are in good condition. PCA analysis showed a correlation between the distribution of mangrove species and parameters of salinity, pH, DO, temperature, depth and brightness.Keywords:MangrovesBreeding SiteHydrologyPlanktonCoastal Area