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All Journal Dentika Dental Journal Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Mutiara Medika: Jurnal Kedokteran dan Kesehatan Majalah Obat Tradisional Indonesian Journal of Cancer Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Jurnal Penelitian Pendidikan IPA (JPPIPA) JCRS (Journal of Community Research and Service) International Journal of Multidisciplinary: Applied Business and Education Research Biofaal Journal Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan IRA Jurnal Pengabdian Kepada Masyarakat Jurnal Bisnis Kehutanan dan Lingkungan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Kalwedo Sains (KASA) Waste Handling and Environmental Monitoring JURNAL REKAYASA KIMIA & LINGKUNGAN

Supriatno

Departemen Ilmu Penyakit Mulut Fakultas Kedokteran Gigi Universitas Gadjah Mada

Author-ID : 393885

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Other

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Sinergi Mahasiswa dan Industri dalam Peningkatan Kompetensi dan Penerapan Teknik Pengecoran Logam di CV Bengkel Bukit Harapan Iswandi, Iswandi; Darianto, Darianto; Hermanto, Tino; Aldhori, Yopan Rahmad; Supriatno, Supriatno; Haniza, Haniza
IRA Jurnal Pengabdian Kepada Masyarakat (IRAJPKM) Vol 3 No 1 (2025): April
Publisher : CV. IRA PUBLISHING

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56862/irajpkm.v3i1.164

Community Service Activities by the Mechanical Engineering Study Program of Medan Area University aim to bridge the world of higher education with the needs of local industry. This activity takes the form of industrial visits and hands-on learning at CV Bengkel Bukit Harapan, a metal casting manufacturing workshop located in Deli Serdang, North Sumatra. The methods used include theoretical counseling, field observation, open discussions, and direct practice. Students gain real-world experience in the metal casting process, from melting and pouring to work safety aspects and product finishing. The interaction between lecturers, students, and industry partners results in a two-way exchange of Knowledge that enriches students' technical insights and practical skills while also providing industry partners with valuable technical input. This activity demonstrates that a field-based collaborative approach enhances the effectiveness of learning and fosters stronger campus-industry partnerships. The results of this activity will serve as a model for sustainable synergy in community empowerment through technical education.

Epidemiologic profile of oral squamous cell carcinoma in Yogyakarta, Indonesia Gracia, Isadora; Utoro, Totok; S., Supriatno; Astuti, Indwiani; Heriyanto, Didik Setyo; Pramono, Dibyo
Padjadjaran Journal of Dentistry Vol 29, No 1 (2017): March 2017
Publisher : Universitas Padjadjaran

ABSTRACTIntroduction: Oral cancer is a major health problem in the world, indicated by a high incidence of recurrence. In Southeast Asia, oral cancer ranks sixth from all cancers on the human body. The incidence of oral cancer, especially squamous cell carcinoma types, increases every year, but the prognosis and patients life expectancy are still unsatisfying enough and the reports about the profile of oral cancer is still limited.The objective of this research was to get the epidemiological profile of oral squamous cell carcinoma (OSCC) in Yogyakarta, Indonesia. Methods: The research method was observational analytic with cross-sectional design. All new OSCC cases in three anatomic pathology laboratory installations in Yogyakarta (January 2011-December 2015) were included in this study. Data collected were gender, age, anatomical location, and histology. All data were analyzed using statistical analysis program. Results: OSCC new cases amount increasing each year: 6 cases (6.6%) in 2011, and increased to 24 cases (26.4%) in 2015. Fifty-two of the 91 cases (57.1%) were found on male. The age group with the most cases was found in group of 41-60 years (49.5%). There is no difference in the case distribution by age between male and female. Forty-eight cases were found in the lingual part (52.7%). Histological examination showed 68 cases (74.7%) were well-differentiated carcinoma. Conclusion: OSCC profile in Yogyakarta mostly found in men with anatomical location in the lingual part in the age group above 40 years of age and were having well-differentiated histology.Keywords: Oral squamous cell carcinoma (OSCC), Epidemiologic profile, Yogyakarta

Docetaxel hydrate induces apoptosis and suppresses tumorigenesis of oral Burkitt’s lymphoma cells (in vitro and in vivo studies) Supriatno, Supriatno
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Universitas Padjadjaran

Introduction: Burkitt’s lymphoma (BL) is one of the tumours with high malignancy and rapid cell growth, derived from B-cell lymphoma. BL typically found in children at dengue-endemic and HIV-AIDS areas with low socioeconomic levels. This study was aimed to analyse the induction of apoptosis and the suppression of tumorigenesis of oral Burkitt’s lymphoma (Raji) cells using docetaxel hydrate in vitro and in vivo. Methods: In the present study, the pure experimental laboratory with post-test only control group design was carried out. Raji cell cultures were incubated with docetaxel hydrate by doses of 0, 1.25 x 10-2, 2.5 x 10-2, and 5.0 x 10-2 M; and IC50 carboplatin (3.1 x 10-6 M) as a positive control. Induction of apoptotic was analysed by double staining of acridine orange-ethidium bromide. Tumorigenesis assay was performed by inoculating Raji cells in nude mice flanks at 1 x 106 cells/mice. Tumour treatment was delivered by various doses of docetaxel hydrate peroral. Results: Apoptosis cells were significantly increased in Raji cells treated with docetaxel hydrate by doses of 2.5 x 10-2 and 5.0 x 10-2 M. The tumour volume in mice given doses of 2.5 x 10-2 and 5.0 x 10-2 M was markedly decreasing compared to control (dose of 0). Conclusion: Docetaxel hydrate has a high antitumour potency by inhibiting tumorigenesis and increasing apoptosis of Burkitt’s lymphoma cells. Keywords: Docetaxel hydrate, double staining, Burkitt’s lymphoma cell, apoptosis, tumorigenesis

Antisense oligonucleotide p45Skp-2 suppresses migratory chemotactic and metastasis of oral malignant Burkitt’s lymphoma cell through down-regulation of MTA-1 and induction of E-cadherin mechanism Supriatno, Supriatno; Irnawati, Dyah; Medawati, Ana
Padjadjaran Journal of Dentistry Vol 32, No 3 (2020): November 2020
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol32no3.21756

Introduction: Burkitt’s lymphoma is a high-grade B-cell neoplasm and one of the most aggressive malignancies of lymphoid origins which found mainly in the paediatric population. The treatment options of this tumour are still limited. However, a new strategy for refractory tumour, phosphorothioate oligonucleotide antisense technique has watched with keen interest. This study was aimed to examine the effect of antisense p45Skp-2 (Skp-2 AS) suppressed migratory chemotactic and metastasis of oral malignant Burkitt’s lymphoma (Raji) cell through down-regulation of MTA-1 and E-cadherin. Methods: True experiment laboratory with post-test control group design was confirmed in this study. The efficiency of Skp-2 AS in the suppression of cell chemotactic migration was examined by Boyden chamber assay. To evaluate the inhibition of cell metastasis was conducted by decreasing MTA-1 expression protein. The expressions of MTA-1, E-cadherin and α-tubulin protein were investigated by Western blot analysis. Results: The results revealed that the number of chemotactic migration of Skp-2 AS treated Raji cell was significantly decreased when compared with that of sense p45Skp-2 (Skp-2 S) and scrambled control (SC) cells (P<0.05) followed by decreased expressions of MTA-1 protein and overexpression of E-cadherin. Interestingly, the expression of α-tubulin protein as an internal control was approximately similar in each transfectant cells. Conclusion: p45Skp-2 have an antitumor activity via suppression of migratory chemotactic activity and metastasis on oral Burkitt’s lymphoma cells through down-regulation of MTA-1 and induction of E-cadherin proteins targeting this molecule could represent a promising new therapeutic approach for this type of cancer.

Caspase-3/-9 as tongue cancer cell apoptosis target induced by ibuprofen Supriatno; Rosanto, Yosaphat Bayu; Karina, Vincensia Maria
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 4 (2025): December
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i4.p355-360

Background: Tongue carcinoma is different from oral cancer in other areas because it has a high amount of cell growth, localized migration, and a higher rate of spreading to cervical lymphatic nodes. Therapeutic alternatives, particularly concerning recurrent malignancies, are notably constrained. Nevertheless, it is imperative to explore novel methodologies for refractory neoplasms, one of which may involve the application of ibuprofen. Purpose: The goal of this study was to look at how well caspase-3 and -9 break down proteins as possible targets for apoptosis in tongue cancer cells that were caused by ibuprofen. Methods: A controlled laboratory experiment employing a post-test-only design was executed. We used a colorimetric test for caspase-3 and -9 to check for the induction of apoptosis. The suppression of cellular invasion was verified through the Boyden chamber assay. Western blot analysis was utilized to identify the presence of caspase-3 and -9 proteins. The administered doses of ibuprofen were calibrated at 0, 5, 10, 25, 50, and 100 µg/mL. Data were subjected to analysis using two-way ANOVA followed by post-hoc Least Significant Different (LSD), with a significance threshold set at 95%. Results: It was established that ibuprofen at dosages ranging from 25 to 100 µg/mL significantly facilitated apoptosis in cells through an augmentation of the proteolytic activity of caspase-3 and -9. Notably, caspase-9 exhibited a superior proteolytic activity (1.85-fold) compared to caspase-3 (1.30-fold) (P = 0.038) at the 100 µg/mL concentration. The upregulation of caspase-3 and -9 proteins was observed in cells treated with ibuprofen. Ultimately, ibuprofen demonstrated an ability to inhibit the invasion of tongue carcinoma cells across various dosages. Conclusion: Ibuprofen has been shown to induce the proteolytic activities of caspase-3 and -9 in tongue carcinoma cells. However, the proteolytic activity of caspase-9 surpassed that of caspase-3, suggesting that targeting this protein may constitute a promising novel therapeutic strategy for this cancer variant.

Workshop Pembuatan Gummy Candy Madu Kelulut Di Desa Bangun Rejo Kabupaten Kutai Kartanegara Samsul, Erwin; Junaidin, Junaidin; Rijai, Laode; Ibrahim, Arsyik; Herman, Herman; Rusli, Rolan; Ahmad, Islamudin; Febrina, Lizma; Farah, Harra Ismi; Hikmawan, Baso Didik; Rija’i, Hifdzur Rashif; Supriatno, Supriatno; Rusman, Arman; Almeida, Maria; Riki, Riki; Arifuddin, M; Bone, Mahfuzun; Arifian, Hanggara; Setiawaty, Agus
Journal of Community Research and Service Vol. 9 No. 2: July (2025)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jcrs.v9i2.69839

A workshop on making gummy candy based on stingless bee honey was held in Bangun Rejo Village, Kutai Kartanegara Regency, as an effort to empower the community through innovation in locally processed food products. The aim of this activity was to improve the skills of MSME actors and village residents in producing stingless honey gummy candy, and to elevate the added value of local honey. The methods used included a short theoretical session about the composition of stingless bee honey and the technical process of gummy candy production, a live demonstration, group practice among participants, as well as evaluation via pre-test and post-test on understanding and skills. The workshop was attended by 50 participants from various MSMEs and community groups. Results showed that after training there was an increase in participant understanding by approximately 75% compared to before the training. Furthermore, participants succeeded in making gummy candy with a chewy texture, the natural sweetness of stingless bee honey, and a distinctive aroma that was well accepted in organoleptic tests. This workshop made a tangible contribution to local product diversification, increasing production capacity, and the potential enhancement of village income through signature products based on stingless bee.

PEMBERIAN EKSTRAK AMPAS DAUN SERAI WANGI (Cymbopogon Nardus (L.) Randle) TERHADAP PERUBAHAN TINGKAH LAKU LARVA INSTAR II Chrysodeixis Chalcites Maulinda, Maulinda; Supriatno, Supriatno; Wardiah, Wardiah; Rahmatan, Hafnati; Sarong, M. Ali
Biofaal Journal Vol 4 No 1 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i1pp20-25

Chrysodeixis chalcites termasuk larva yang sangat khas, karena cara berjalan berjingkat-jingkat, yaitu dengan cara ujung bagian belakang tubuhnya melengkung, kemudian tubuh bagian depan bergerak maju. Daun serai wangi mengandung zat aktif seperti alkaloid, saponin, tanin, dan flavonoid. Komponen utama dari minyak serai wangi yaitu sitronela dan geraniol. Ekstrak ampas daun serai wangi bepotensi sebagai insektisida alami karena memiliki kandungan senyawa metabolit sekunder berupa alkaloid, saponin, tanin dan flavonoid, senyawa tersebut dapat mempengaruhi tingkah laku larva. Tujuan penelitian ini untuk mengetahui pengaruh pemberian ekstrak ampas daun serai wangi terhadap tingkah laku larva Instar II Chrysodeixis chalcites. Jenis penelitian eksperimen dengan pola Rancangan Acak Lengkap (RAL) dengan 6 perlakuan masing-masing 4 ulangan. Perlakuan yang digunakan adalah kontrol negatif), 5000, 10.000, 15.000. Simpulan dari penelitian ini ekstrak ampas daun serai wangi menyebabkan perubahan tingkah laku pada larva Chrysodeixis chalchites seperti aktivitas makan yang menurun, kegelisahan larva yang bergerak naik turun pada wadah penelitian dan aktivitas bergerak larva yang awalnya bergerak lincah lama kelamaan menjadi lamban hingga sulit bergerak.

Analisis greenwashing pada LSM menggunakan teori dan konsep sustainable management Supriatno
Waste Handling and Environmental Monitoring Vol. 1 No. 1: (February) 2024
Publisher : Institute for Advanced Science, Social, and Sustainable Future

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61511/whem.v1i1.2024.708

Greenwashing is a practice in which businesses or companies present false or misleading information about their sustainability initiatives for marketing purposes. This occurs when companies communicate positive environmental actions while hiding negative ones, creating a misleading impression of overall environmental performance. Greenwashing can occur through two actions: falsification (active deceit) and information selection (passive deceit). Information selection involves strategically choosing environmental communication content to eliminate negative information, while falsification involves creating false communication content. To avoid greenwashing, parameters such as data transparency, equal treatment, environmental focus, water efficiency, carbon footprint, waste packaging, and fair wages can be analyzed for an entity. Additionally, guidelines from the United States Federal Trade Commission (US-FTC) can also help differentiate between greenwashing practices and sustainable practices. However, in certain cases such as the Lindungi Hutan crowdfunding platform, there are several issues hindering environmental conservation efforts. The crowdfunding scheme used does not check the background of companies or individuals donating, allowing donations to come from businesses that have negative impacts on the environment. Additionally, the lack of annual public reports reduces transparency and accountability in fund management. Although this platform has various activities for environmental conservation, the effectiveness and impact of these activities are not well disclosed. Furthermore, claims from donors about their participation in environmental care programs are not aligned with the sustainability principles that should be followed. In conclusion, companies should focus on internal improvements and adhere to sustainability principles to avoid greenwashing. Additionally, crowdfunding platforms like Lindungi Hutan need to enhance transparency, accountability, and the effectiveness of their programs to ensure a real contribution to environmental preservation.

Ethanol extract of mangosteen (Garcinia Mangostana Linn) peel effect in inhibiting the growth of human tongue cancer cells Supri’s Clone 1, invitro Suanto, Edi; Oewen, Roosje Rosita; Sasmita, Inne Suherna; Supriatno, S.; Supratman, Unang
Padjadjaran Journal of Dentistry Vol 23, No 2 (2011): July 2011
Publisher : Universitas Padjadjaran

The incidence of tongue cancer in Indonesia reached 1.01% of all cancers and 42% of oral cavity cancer. Tongue cancer therapies including chemotherapy, radiotherapy, surgery, and all three combined therapy. Search for anti-cancer drugs currently switched on herbal plants, one of which is the mangosteen. Has the properties of mangosteen peel extract inhibited the growth of cancer cells. The purpose of the study, obtain IC50 of ethanol extract of mangosteen peel in inhibiting the growth of human tongue cancer cells SP-C1. Research carried out on 96 preparations of human tongue cancer SP-C1 were incubated with ethanol extract of mangosteen peel, preparations were classified in two groups of incubation time (24 hours and 48 hours) and each group will be given preferential treatment over 6 randomly different concentrations: 0 (control), 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL and 1000 μg/mL. Model experiments were 2 x 6 factorial experiment with eight replication for each cell. Test results with ANAVA, incubation (24 and 48 hour) SP-tongue cancer cells with various concentrations of C1 ethanol extract of mangosteen peel gives a highly significant, indicating differences cancer cell growth inhibition. Incubation time factor showed the long incubation effect on cancer cell growth inhibition. Furthermore, by Newman Keuls test, showed 500μg/mL concentrations of 24-hour incubation had the best effect. Conclusion of the study of ethanol extract of mangosteen peel could achieve with IC50 values of cell growth resistance 50.3% at a concentration of 500 μg/mL and an incubation time of 24 hours.

Distribution pattern of Pb and Zn contamination in rivers near industrial zone in Aceh, Indonesia, revealed by principal component analysis (PCA) Lelifajri, Lelifajri; Rahmadi, Rahmadi; Idroes, Rinaldi; Supriatno, Supriatno; Safitri, Eka; Nazaruddin, Nazaruddin; Maulana, Ilham; Suhud, Khairi
Jurnal Rekayasa Kimia & Lingkungan Vol 17, No 1 (2022): Jurnal Rekayasa Kimia & Lingkungan (June, 2022)
Publisher : Chemical Engineering Department, Syiah Kuala University, Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23955/rkl.v17i1.22672

The research aims to evaluate the pollution of Pb and Zn in water, sediment, and Faunus ater samples collected from Krueng Balee (KB) and Kreung Reuleung (KR) Rivers, Aceh, Indonesia. The samples were collected at the upstream, midstream, and downstream of each river and analyzed using Atomic Absorption Spectrophotometry (AAS). The distribution of Pb and Zn pollution was analyzed using Principal Component Analysis (PCA). Our investigation revealed that the presence of Pb and Zn is still safe for water and sediment samples. Nevertheless, Pb and Zn contamination found in F. ater samples had exceeded the national safety limit for food. PCA analysis revealed correlations between samples suggesting the roles of physical and chemical properties of the river in the pollutant retainment. The analysis also indicated the possible antagonism between Pb and Zn accumulation in F. ater which has never been reported previously. We suggest routine monitoring of Pb and Zn concentrations. The role of the surrounding industry in the Pb and Zn pollution should be further studied.

Co-Authors Abdullah - Achmad Syawqie Agus Setiawaty Aldhori, Yopan Rahmad Ali Sarong Almeida, Maria Ana Medawati, Ana Andi Ulfa Tenri Pada Arifian, Hanggara Arifuddin, M Atiek Driana Rahmawati, Atiek Bone, Mahfuzun Darianto, Darianto Dibyo Pramono Didik Setyo Heriyanto Djufri - Dyah Irnawati Edi Suanto, Edi Eka Safitri Eriska Riyanti Farah, Harra Ismi Febrina, Lizma Fimma Naritasari Gina Arfianti Putri, Gina Arfianti Hafnati Rahmatan Haniza Harun Achmad, Harun Hendri Susanto Herman Herman Hikmawan, Baso Didik Himmaturojuli Rosyid Ridlo, Himmaturojuli Ibrahim, Arsyik Ilham Maulana Indwiani Astuti Inne Suherna Sasmita Isadora Gracia, Isadora Islamudin Ahmad Ismul Huda Iswandi Iswandi Junaidin, Junaidin Keri Pangesti Yudi Harhari, Keri Pangesti Yudi Kharisma, Irma Laode Rijai Lelifajri Lelifajri Martono, Dwi Nowo Maulinda, Maulinda Maureen Martina, Maureen Mieke Hemiawati Satari N. Nazaruddin Nur, Yaumil Istiqlal M. Rahmadi Rahmadi Rija’i, Hifdzur Rashif RIKI RIKI, RIKI Rinaldi Idroes Riska Riska Roosje Rosita Oewen, Roosje Rosita Rusli, Rolan Rusman, Arman Safrida Safrida Safrida Samsul, Erwin Sapurti, Mimie Sri Budiarti Poerwanto Suhud, Khairi Tino Hermanto Totok Utoro Ulhusna, Fitra Asma Ulva, Susi Mulia Unang Supratman Vincensia Maria Karina Wardiah Wardiah Yosaphat Bayu Rosanto Yuliani, Wirda Zakiyana, Yulida

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