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The effect of combination of active fraction Andrographis paniculata (Burm.f) Ness and Centella asiatica (i) Urban on the alpha glucocidase inhibitor and antioxidant activities Arman Rusman; Agung Endro Nugroho; Suwidjiyo Pramono
Indonesian Journal of Pharmacy Vol 31 No 1, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss1pp56

Abstract

The Andrographis panicullata and Centella asistica extract have been reported that had a anti-diabetic effect. However, the specific mechanism and the effect combination of both were not yet reported. This study was purposed to determine the potency of extract, fractions and the combination of Andrographis panicullata (AP) and Centella asistica (CA) active fraction to inhibit alpha glucosidase enzymeand its ability to reduce DPPH radical.AP and CA were extracted using 50% ethanol then fractionated with solvents under different polarity levels. The inhibiting activity to alpha glucosidase enzyme and antioxidant activity of each fractions was tested. The most active fractions from AP and CA were then combined and re-tested for activity. The results result reported that both of AP and CA had inhibition of alpha glucosidase activity and antioxidant activity. Based on calculation combination index (CI) of of active fraction of AP and CA showing in alpha glucocidase activity had a antagonist action and antioxidant had a sinergic action. Therefore, combination of AP and CA not has not recomended for alpha glucocidase inhibitor but the combination has ability to reduce DPPH radical.
Empowerment and training in making herbal tea to utilize herbal plants and prevent degenerative diseases Herman Herman; Andi Tenri Kawareng; Arman Rusman
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol. 9 No. 1 (2024): February 2024
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v9i1.12132

Abstract

Teluk Pemedas is one of the sub-districts in the Samboja sub-district, Kutai Kartanegara, East Kalimantan Province. Previous health examination data showed that the presentation of hypertension in the Teluk Pamedas community was 28% and hypercholesterolemia was 20%. The use of herbal plants as an alternative treatment has been widely used. Plants that have antihypertensive and antihypercholesterolemic activity are rosella flowers, pandan leaves, and cinnamon. Research results show that this plant can lower blood pressure for hypertension sufferers. The aim of carrying out this activity is to increase public knowledge about degenerative diseases and the use of local herbal plants to add economic value. This activity was carried out in two stages, with an education method regarding degenerative diseases, then continued with a training method for making herbal tea preparations from a combination of rosella flowers (Hibiscus sabdariffa), pandan leaves (Pandanus amaryllifolius) and cinnamon (Cinnamomum burmanii). The partner of this activity is Dasawisma Teluk Pamedas sub-district, Samboja, Kutai Kartanegara East Kalimantan. This activity provides benefits to the community regarding efforts to prevent degenerative diseases and implement a healthy lifestyle and improve skills in processing herbal plants.
Kajian Praklinis Ekstrak Etanol Herba Tridax procumbens (Linn.) sebagai Antidiabetik pada Tikus Putih Diabetes (Rattus norvegicus) yang Diinduksi Streptozotosin: Preclinical Study of Ethanol Extract of Herba Tridax procumbens (Linn.) as Antidiabetic in Streptozotocin-Induced Diabetic White Rats (Rattus norvegicus) Syaiful Katadi; Irman Idrus; Arman Rusman; Sitti Sarfila
Jurnal Sains dan Kesehatan Vol. 5 No. 1 (2023): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v5i1.1597

Abstract

This study aims to conduct a preclinical study of the antidiabetic effect of the ethanol extract of the herb Tridax procumbens (Asteraceae) in streptozotocin-induced diabetic rats. The research procedure began by collecting test samples of the Tridax procumbens Linn (TP) herb. All parts of the TP plant which were air-dried were extracted with ethanol (95%) by maceration for 72 hours, then phytochemical screening was carried out to determine the content of secondary metabolites in general. The antidiabetic test was initiated by inducing male Wistar rats with streptozotocin (50 mg/jk, i.p.) and nicotinamide (120 mg/kg, i.p.) by injection. Diabetic rats were treated with glibenclamide (0.25 mg/kg, po) and TP extract (250 and 500 mg/kg, p.o.) for 21 consecutive days. Blood samples were collected periodically to measure the hypoglycemic effect of the TP extract. Then, variations in body weight at the fair were monitored for all experimental animals. The results showed that TP extract had a significant antidiabetic effect on diabetic white rats (Rattus norvegicus), with the best results given TP extract at a dose of 500 mg/kg. Keywords: Tridax procumbens, diabetes mellitus, antidiabetic Abstrak Penelitian ini bertujuan untuk melakukan kajian praklinis efek antidiabetes dari ekstrak etanol herba Tridax procumbens (Asteraceae) pada tikus diabetes yang diinduksi streptozotocin. Prosedur penelitian dimulai dengan mengumpulkan sampel uji herba Tridax procumbens Linn (TP). Seluruh bagian tanaman TP yang telah dikeringkan diekstraksi dengan etanol (95%) dengan cara dimaserasi selama 72 jam, selanjutnya dilakukan skrining fitokimia untuk mengetahui kandungan metabolit sekundernya secara umum. Untuk Uji antidiabetes dimulai dengan menginduksi tikus Wistar jantan dengan streptozotocin (50 mg/kg, ip) dan nicotinamide (120 mg/kg, ip). Tikus diabetes diobati dengan glibenklamid (0,25 mg/kg, po) dan ekstrak TP (250 dan 500 mg/kg, p.o.) selama 21 hari berturut-turut. Sampel darah dikumpulkan secara berkala untuk mengukur efek hipoglikemik ektrak TP. Kemudian, variasi berat badan mingguan dipantau untuk semua hewan percobaan. Hasil penelitian menunjukkan ekstrak TP memiliki efek antidiabetik yang signifikan terhadap tikus putih diabetes (Rattus norvegicus), dengan hasil terbaik pada pemberian ekstrak TP dosis 500 mg/KgBB. Kata Kunci: Tridax procumbens, diabetes mellitus, antidiabetik
Karakterisasi Ekstrak Sambiloto (Andrographis panicullata Burn (f) Ness) dan Pegagan (Centella asiatica (l) Urban): Characterization Extract Sambiloto (Andrographis panicullata Burn (f) Ness) and Pegagan (Centella asiatica (l) Urban) Arman Rusman; Agung Endro Nugroho; Suwijiyo Pramono; Herman Herman; Muhammad Faisal; Junaidin Junaidin; Haeruddin Haeruddin
Jurnal Sains dan Kesehatan Vol. 5 No. 2 (2023): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v5i2.1749

Abstract

Sambiloto (Andrographis panicullata Burn (f) Ness) and Pegagan (Centella asiatica (l) Urban) are plants that have many benefits. Traditionally this plant is used as a medicine including insect bites and venomous snakes, dysentery, diabetes, venereal diseases, appendicitis, dirty blood, eczema, tonsil inflammation, ulcers and food poisoning. This study aims to determine the characterization of bitter and gotu kola extracts obtained from Girimulyo Village, Nanggulan District, Kulon Progo Regency which includes yield, drying loss, TLC profile, active compound content, and total flavonoid content. The extract characterization method follows the guidelines written in the Indonesian herbal famacopoeia (FHI). The results showed that the extracts of Sambiloto and Centella asiatica obtained the following data: the yield value of Sambiloto extract was 18.9% and Centella asiatica 21.7%, the organoleptic examination of the data obtained was in accordance with FHI standards, the drying shrinkage value of Sambiloto was 7.85 ± 0.60 and Centella asiatica 15.15 ± 0.42, qualitative analysis of Sambiloto and Centella asiatica extracts had the same rf values as the marker compounds, namely andrographolide for Sambiloto and asiaticoside for Centella asiatica. Measurement of andrographolid levels in Sambiloto extract showed a value of 32.56 ± 4.56 and Asiaticoside levels gotu kola extract showed a value of 15.01 ± 1.69. These results show that the bitter extracts and gotu kola taken from Girimulyo Village, Nanggulan District, Kulon Progo Regency comply with the standards written in the Indonesian herbal pharmacopoeia (FHI). Keywords: Sambiloto, Pegagan, Extract, Characterization Abstrak Sambiloto (Andrographis panicullata Burn (f) Ness) dan Pegagan (Centella asiatica (l) Urban) merupakan tanaman yang memiliki banyak manfaat. Secara tradisional tanaman ini digunakan sebagai obat diantaranya gigitan serangga dan ular berbisa, disentri, kencing manis, penyakit kelamin, radang usus buntu, darah kotor, eksema, radang tonsil, borok dan keracunan makanan. Penelitian ini bertujuan untuk mengetahui karakterisasi dari ekstrak sambiloto dan pegagan yang diperoleh dari Desa Girimulyo, Kecamatan Nanggulan, Kabupaten Kulon Progo yang meliputi rendemen, susut penegringan, profil KLT, kadar senyawa aktif, dan kadar flavonoid total. Metode karakterisasi ekstrak mengikuti panduan yang tertulis dalam famakope herbal Indonesia (FHI). Hasil penelitian menunjukan ekstrak sambiloto dan pegagan diperoleh data sebagai berikut: nilai rendemen ekstrak sambiloto 18,9 % dan pegagan 21,7%, pemeriksaan organoleptik data yang diperoleh sesuai dengan standar FHI, nilai susut pengeringan sambiloto 7,85±0,60% dan pegagan 15,15±0,42%, analisis kualitatif ekstrak sambiloto dan pegagan memiliki nilai rf yang sama dengan senyawa penanda yakni andrografolid untuk sambiloto dan asiaticosida untuk pegagan, pengukuran kadar andrografolid pada ekstrak sambiloto menunjukan nilai 32,56±4,56% dan kadar asiaticosida pada ekstrak pegagan menunjukan nilai 15,01±1,69%. Hasil tersebut menunjukan bahwa ekstrak sambiloto dan pegagan yang diambil dari Desa Girimulyo, Kecamatan Nanggulan, Kabupaten Kulon Progo sesuai dengan standar yang tertulis dalam farmakope herbal Indonesia (FHI). Kata Kunci: Sambiloto, Pegagan, Ekstrak, Karakterisasi
Antioxidant Activity of Bajakah Kuning (Arcangelisia flava (L.) Merr) Herman, Herman; Almaida, Maria; Haeruddin, Haeruddin; Bone, Mahfuzun; Rusman, Arman
Journal of Tropical Pharmacy and Chemistry Vol. 8 No. 2 (2024): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v8i2.652

Abstract

Indonesia is a tropical country with potential plants that have been used as traditional medicine for generations. One of them is bajakah. This study aims to determine the phenolic content and antioxidant activity of yellow bajakah extract and the relationship between phenolic content and antioxidant activity. The study began with making yellow bajakah extract using maceration method. The bajakah extract obtained then analysed the phenolic content quantitatively using the Folin-Ciocalteu method. Antioxidant activity was tested using the DPPH and ABTS methods. The results showed that yellow bajakah extract contained phenolic compounds with levels of 27.15 ± 1.28 mg/g GAE. IC50 measurements to determine antioxidant activity obtained a value of 47.01 ± 1.16 µg / ml in the DPPH method and 61.21 ± 0.83 µg / ml in the ABTS method. Based on these results, yellow bajakah skin is known to have antioxidant activity with a strong category and phenolic content in yellow bajakah skin has an influence on antioxidant activity.
Aktivitas Antioksidan Ekstrak Kulit dan Daging Buah Labu Siam (Sechium edule Sw.) Terhadap Radikal Bebas DPPH Ibrahim, Arsyik; Irwansyah, Irwansyah; Rusman, Arman; Badawi, Satriani; Riki; Rija'i, Hifdzur Rashif; Fadraersada, Jaka
Jurnal Riseta Naturafarm Vol. 1 No. 2 (2024): Jurnal Riseta Naturafarm
Publisher : B-Creta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70392/jrn.v1i2.4652

Abstract

 Chayote is a vines type of plant, and usually people known it as a healthy food and its fruit can be cooked as a vegetable soup. Research aim was to find out the activity from rind and fruit flesh extract of chayote (Sechium edule Sw.) as an antioxidant and find out IC50 from rind and fruit flesh extract of chayote S. edule Sw. After peel the rind of chayote fruit, then chayote rind extracted with soxhletation method and chayote fruit flesh with maceration method. Antioxidant activity evaluated by using DPPH method with spectrophotometry, and IC50 count using regresi linier analysis. This research used methanol extract of chayote rind and methanol extract of chayote fruit flesh. Research results showed that chayote rind extract and chayote fruit flesh have some activity as an antioxidant. IC50 for each sample is 422,38 ppm for methanol extract of chayote rind and 1322,62 ppm for methanol extract of chayote fruit flesh.
Secondary Metabolites and Cytotoxicity of Kitolod Leaf Extract (Hippobroma longiflora (L.) G. Don to Shrimp Larvae (Artemia salina Leach): Metabolit Sekunder dan Sitotoksisitas Ekstrak Daun Kitolod (Hippobroma longiflora (L.)) terhadap Larva Udang (Artemia salina Leach) Ibrahim, Arsyik; Bulan, Adisty Sindi; Ramadhan, M. Rahmad; Bone, Mahfuzun; Rija'i, Hifdzur Rashif; Rusman, Arman; Arifuddin, M.; Junaidin, Junaidin; Rijai, Laode
Jurnal Riseta Naturafarm Vol. 2 No. 1 (2025): Jurnal Riseta Naturafarm
Publisher : B-Creta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70392/jrn.v2i1.3342

Abstract

One of the plants used by the people of East Kalimantan, especially the people of West Kutai, as a medicinal plant is Kitolod (Hippobroma longiflora L.). The plant parts used are the leaves and flowers. The secondary metabolites contained in Kitolod leaves are alkaloids, saponins, flavonoids and polyphenols. The aim of this research is to identify secondary metabolite groups and test the cytotoxicity of Kitolod leaf extracts and fractions. Secondary metabolites are identified qualitatively using various chemical reagents and metabolites characterized by the presence or absence of color changes or the presence of sediment or foam. Cytotoxicity testing was carried out using the Brine Shrimp Lethality Test (BSLT) method. The data research was analyzed using the Reed and Muench method to determine the Lethality Concentration 50% value. The results of research on the metabolite content of ethanol extract, fractions: n-hexane, ethyl acetate and n-butanol contain alkaloid, flavonoids and phenolic/polyphenols compounds. The bioactivity test results for the ethanol extract had an LC50 value of 592.93 ppm, the n-hexane fractions of 582.77 ppm, the ethyl acetate fraction of 531.37 ppm, and the n-butanol fraction of 119.48 ppm. These results indicate that the n-butanol fraction of Kitolod leaves has stronger cytotoxic activity than other extracts. .
Upaya Peningkatan Kesehatan Melalui Pemeriksaan Kesehatan Gratis pada Masyarakat Desa Bangun Rejo Kecamatan Tenggarong Seberang Kabupaten Kutai Kartanegara sebagai Upaya Deteksi Dini Penyakit Tidak Menular Arifuddin, Muhammad; Samsul, Erwin; Riki; Rija'i, Hifdzur Rashif; Rusli, Rolan; Ibrahim, Arsyik; Febrina, Lizma; Bone, Mahfuzun; Junaidin; Herman; Rusman, Arman; Hikmawan, Baso Didik; Ahmad, Islamudin
Jurnal Abdita Naturafarm Vol. 2 No. 1 (2025): J Abdita Naturafarm
Publisher : B-Creta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70392/jan.v2i1.1925

Abstract

Non-communicable diseases (NCDs) have become a significant global health challenge. Conditions such as heart disease, diabetes, and hypertension contribute to more than 70% of deaths worldwide. This community service activity aims to provide free health screenings to the community, particularly to the residents of Desa Bangun Rejo, with the goal of early detection of NCDs. The community service activities involved health screenings followed by direct education to the participants. The results showed that 40 participants attended and gained knowledge about their current health conditions, including blood pressure, blood glucose levels, and uric acid levels. The findings from the health screenings will serve as a reference for participants to understand the importance of regular health check-ups in mitigating future risks Penyakit tidak menular (PTM) menjadi salah satu tantangan kesehatan global yang signifikan. termasuk penyakit jantung, diabetes, dan hipertensi menyumbang lebih dari 70% kematian di seluruh dunia. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk memberikan pemeriksaan Kesehatan gratis kepada masyarakat khususnya masyarakat Desa Bangun Rejo yang bertujuan untuk deteksi dini PTM. Metode kegiatan pengabdian yang dilakukan berupa pemeriksaan Kesehatan dan dilanjutkan pemberian edukasi langsung kepada peserta. Hasil kegiatan pengabdian kepada masyarakat ini menunjukkan peserta yang hadir sebanyak 40 orang mengetahui tentang kondisi saat ini berupa tekanan darah, kadar glukosa darah, dan asam urat. Hasil pemeriksaan yang didapatkan dijadikan sebagai rujukan untuk peserta lebih memahami pentingnya melakukan pemeriksaan kesehatan secara rutin guna menanggulangi resiko yang lebih besar untuk kedepannya
An Innovative Strategy for the Green Synthesis of Nanochitosan Using Plant Extracts and Their Possible Applications: A Review Riki, Riki; Ahmad, Islamudin; Herman, Herman; Ibrahim, Arsyik; Rusman, Arman; Samsul, Erwin; Rusli, Rolan; Arifuddin, Muhammad; Junaidin, Junaidin; Bone, Mahfuzun; Rijai, Hifdzur Rashif; Hikmawan, Baso Didik
Indonesian Journal of Chemistry Vol 25, No 2 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.100148

Abstract

The numerous applications of nanochitosan in a variety of sectors, including medicine, food, and agriculture, attract researchers to work toward the development of environmentally favorable, safe, and efficient synthesis methods. Currently, a biological approach is being developed to synthesize nanochitosan to address the limitations of conventional methods. The synthesis of biogenic pathways employs biological entities, including plant extracts and microorganisms, as nanoparticle-forming agents. The use of extracts is more desirable due to the simple working procedure, the absence of hazardous chemicals, the economic benefits, and the use of basic equipment. This article highlights the role of biomolecules in plant extracts, including polyphenols, terpenoids, citric acid, alkaloids, and polysaccharides, in the synthesis of nanochitosan. Nanoparticle preparation protocols and characterization using zeta potential analysis techniques, scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-visible spectroscopy, and Fourier transform infrared spectroscopy (FTIR) are briefly described. The potential applications of green nanochitosan are also discussed, considering the results of biological activity testing and the potential mechanisms associated with these activities. According to the results of numerous studies highlighted in this review, nanochitosan synthesis utilizing plant extracts appears to be a promising alternative approach to conventional methods.
Aktivitas Antihiperglikemia Infusa Daun Sambung Nyawa (Gynura Procumbens) dengan Metode Toleransi Glukosa pada Mencit Jantan (Mus Musculus): Antihyperglycemic Activities of Sambung Nyawa Leaves (Gynura Procumbens) Infusion using a Glucose Tolerance Method in Male Mice (Mus Musculus) Ridhotulloh, Yusuf Isro; Rijai, Laode; Mus, Nurul Muhlisa; Arifuddin, M.; Ibrahim, Arsyik; Ahmad, Islamudin; Herman, Herman; Hikmawan, Baso Didik; Junaidin, Junaidin; Febrina, Lizma; Faisal, Muhammad; Rusman, Arman; Riki, Riki; Almeida, Maria; Bone, Mahfuzun; Arifian, Hanggara; Samsul, Erwin
Jurnal Sains dan Kesehatan Vol. 7 No. 2 (2025): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v7i2.2421

Abstract

Hyperglycemia is a condition where blood sugar increases significantly which can lead to diabetes mellitus. Sambung Nyawa (Gynura Procumbens) leaves are a plant that is empirically believed to reduce hyperglycemia and has been scientifically proven based on several studies, however there has been no research that provides information about sembung nyawa leaves in infusion form. This study aims to determine the content of secondary metabolites in the infusion of the leaves of Gynura Procumbens and its antihyperglycemia activity in male males. This study consisted of phytochemical and antihyperglycemia screening using a tolerance method induced by glucose monohydrate at a dose of 2g/kgBW. The test group consisted of a negative control group (Na CMC 1%), a positif group (Glibenclamide), and 3 groups of sambung nyawa leaf infusion (5%, 10%, 15%). The results of the research showed that the infusion of sembung nyawa leaves contained secondary metabolites, namely tannins, phenolics, flavonoids and saponins. The effect of reducing blood sugar levels in test animals showed a significant reduction (0.00<0.05) at a 10% concentration infusion compared to the negative control and not significant to the positive control. The conclusion shows that the infusion of sambung nyawa leaves has an antihyperglycemic effect. Keywords:          Antihyperglycemic, Sambung Nyawa Leaves, Glucose Monohydrate   Abstrak Hiperglikemia merupakan kondisi peningkatan gula darah yang cukup signifikan yang akan berubah menjadi kondisi diabetes mellitus. Daun sambung nyawa (Gynura Procumbens) merupakan tumbuhan yang dipercaya secara empiris menurunkan hiperglikemia dan terbukti secara ilmiah berdasarkan beberapa penelitian dalam bentuk ekstrak. Penelitian ini bertujuan mengetahui kandungan metabolit sekunder pada infusa daun sambung nyawa dan aktivitas antihiperglikemia pada mencit jantan. Penelitian ini terdiri dari skrining fitokimia dan antihiperglikemia menggunakan metode toleransi yang dinduksi glukosa monohidrat dengan dosis 2g/kgBB. Kelompok uji terdiri dari kelompok kontrol negatif (Na CMC 1%), positif (Glibenklamid), dan 3 kelompok infusa daun sembung nyawa (5%, 10%, 15%). Hasil penelitian menunjukkan infusa daun sambung nyawa mengandung metabolit sekunder yaitu tanin, fenolik, flavonoid dan saponin. Efek penurunan kadar gula darah pada hewan uji menunjukkan penurunan yang signifikan (0,00<0,05) pada infusa konsentrasi 10% dibandingkan dengan kontrol negatif dan tidak signifikan terhadap kontrol positif. Kesimpulan menunjukkan bahwa infusa daun sambung nyawa mempunyai efek antihiperglikemia. Kata Kunci:         Antihiperglikemia, Daun Sambung Nyawa, Glukosa Monohidrat