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All Journal BULETIN OSEANOGRAFI MARINA Journal of Marine Research AL KAUNIYAH Jurnal Sains dan Teknologi Jurnal Kelautan Tropis JURNAL ENGGANO Dinamisia: Jurnal Pengabdian Kepada Masyarakat Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan Jurnal Akuakultur Sungai dan Danau Bina Hukum Lingkungan CARADDE: Jurnal Pengabdian Kepada Masyarakat Samakia: Jurnal Ilmu Perikanan Techno-Fish JOURNAL OF INDONESIAN TROPICAL FISHERIES (JOINT-FISH) : Jurnal Akuakultur, Teknologi Dan Manajemen Perikanan Tangkap, Ilmu Kelautan Abdihaz: Jurnal Ilmiah Pengabdian pada Masyarakat Reswara: Jurnal Pengabdian Kepada Masyarakat Society : Jurnal Pengabdian dan Pemberdayaan Masyarakat PAKDEMAS : Jurnal Pengabdian Kepada Masyarakat Bina Hukum Lingkungan JURNAL BESEMAH Manfish Journal Journal of Fisheries & Marine
Risnita Tri Utami
Universitas Prof. Dr. Hazairin, SH
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Journal : AL KAUNIYAH

 1 
Identifikasi dan Analisis Filogentik Kerang Kima (Genus Tridacna) dari Pulau Kur Menggunakan DNA Barcoding Teddy Triandiza; Agus Kusnadi; Hawis Madduppa; Neviaty P Zamani; Udhi E Hernawan; Rosmi N Pesillette; Abdul Kadir Yamko; Nurlita P Anggraini; Fildzah Z Hulwani; Ichtineza H Hardono; Risnita Tri Utami
Al-Kauniyah: Jurnal Biologi Vol 15, No 1 (2022): AL-KAUNIYAH: JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v15i1.17631

Abstract

AbstrakPeran penting kerang Kima secara ekologis sebagai salah satu spesies kunci dalam ekosistem terumbu karang menyebabkan biota laut ini mengalami tekanan antropogenik di sebagian besar wilayah di Indo-Pasifik, termasuk Indonesia. Penelitian ini bertujuan untuk mengidentifikasi spesies Kima genus Tridacna asal Pulau Kur menggunakan markea gen COI dan menganalisis hubungan filogenetiknya. Sampel jaringan Kima diekstrak menggunakan kit ekstraksi komersial. Primer universal (LCO1490; HCO2198) dan primer spesifik Kima (LCO: 5’-GGG TGA TAA TTC GAA CAG AA-3’; RCO: 5’-TAG TTA AAG CCC CAG CTA AA-3’) digunakan untuk mengamplifikasi DNA target. Analisis data dilakukan terhadap 26 sekuen Kima asal Pulau Kur dengan panjang fragmen 479 bp menggunakan software MEGA6 meliputi analisis jarak genetik dan pohon filogenetik. Hasil analisis homologi menggunakan BLASTn mendapatkan empat jenis Kima, yaitu Tridacna maxima, T. crocea, T. noae, dan T. squamosa dengan similaritas 99–100%. Hasil penelitian menunjukkan bahwa jarak genetik dalam spesies sangat rendah berkisar 0,0000–0,0103 sedangkan jarak genetik antar spesies berkisar antara 0,0112–0,1729. Hasil konstruksi pohon filogenetik kerang Kima menunjukkan adanya dua klad utama yang kohesif dan jelas terpisah, yaitu kelompok Tridacna yang monofiletik dan Hippopus hippopus. Berdasarkan hasil penelitian, teknik DNA Barcoding sangat efektif untuk identifikasi dan mengkonfirmasi fenomena cryptic spesies.Abstract The role of the giant clam as the key and ecologically important species in coral reef ecosystem has led them to experience anthropogenic pressure in the most of Indo-pacific region including Indonesia. This research aimed to identify the species of giant clam Tridacna from Kur Island, Southeast Maluku by using COI gene marker and to analyze their phylogenetic relationship. The tissue sample of giant clam was extracted with a commercial extraction kit. Universal primer (LCO1490; HCO2198) and specific primer for giant clam (LCO: 5’-GGG TGA TAA TTC GAA CAG AA-3’; RCO: 5’-TAG TT AAG CCC CAG CTA AA-3’) was used to amplify DNA target. Twenty-six sequences from Kur Island with 479 bp fragment length were analyzed by using MEGA6 software. Homological analysis on BLASTn found four species i.e Tridacna maxima, T. crocea, T. noae, and T. squamosa with 99–100% similarity. The result also showed that genetic distance value intraspecies was very low ranging from 0.000 to 0.0105, while genetic distance inter-species ranged from 0.0112 to 0.1729. Reconstruction of phylogenetic tree of giant clam revealed two main group that cohesive and clearly separated i.e Tridacna group which monophyletic and Hippopus hippopuxs. Based on the result, the DNA barcoding technique was effective for identification and confirmation of the occurrence of cryptic species phenomenon.
Co-Authors Abdul Kadir Yamko Adam Suhanto Afan Mahmudin Agus Kusnadi Agus Kusnadi Aida Sartimbul Ami Nolisa An Nisa Nurul Suci Ari Anggoro Arif Prasetya Aulia Salsabilla, Zahra Ayub Sugara Dedi Pardiansyah Dedy Kurnianto Delvia Novitasari Dwi Oktavallyan Elvina, Wica Engga Jalaludin Erlangga Erlangga Esty Kurniawati Fahresa Nugraheni Supadminingsih, Fahresa Nugraheni Febrina Rolin Feri Nugroho Fildzah Z Hulwani Firman Firman Fitra Wira Hadinata Hadinata, Fitra Wira Handoko, Lugu Tri Handoko Hasidu, La Ode Abdul Fajar Hawis H Madduppa Hayatun Nufus Hendrik V. Ayhuan Herry Novrianda Ichtineza H Hardono Janusi Waliamin Julian Saputra Laode Muhamad Hazairin Nadia Leni, Yusyam Maharani Marlinah Marlinah Melisa Mimit Primyastanto Nella Tri Agustini, Nella Tri Neviaty P Zamani Nur Anisa Nur Ikhsan Nur Safira Nurlita P Anggraini Nurseha Nurseha Okka Adittio Putra Parwito Pesilette, Rosmi Nuslah Puspita, Veny Putra Akhir, Ade Fitrah Putri Sapira Ibrahim Rahayu, Andra Rejekineng Rifi Suhendri Rika Anggraini Rika Anggraini Riri Ezraneti Rosmi N Pesillette Rosmi N Pesillette Sherly Nelsa Fitri Silvy Syukhriani, Silvy Sintia Safrianti Sri Sedjati Sri Sedjati Sriwidodo, Maheno Suara Ningsih, Aisyah Suhanto, Adam Suharun Martudi Sunaryo Sunaryo Sunaryo Sunaryo Surbakti, Livia Chasinta SYAHRIAL SYAHRIAL Syahrial Syahrial Teddy Triandiza Teddy Triandiza Teddy TRIANDIZA Udhi E Hernawan Wahyu Alfanda Wediyanto, Eko Wica Elvina Wica Elvina Wica Elvina Wiwit Pratiwi, Wiwit Yudho Andika Yudisky Yudisky Yudisky, Yudisky Yulfiperius, Yulfiperius Zulkhasyni