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Journal : AGRIVITA, Journal of Agricultural Science

AN EFFECTIVE METHOD FOR DNA EXTRACTION OF MATURE LEAF OF SAPODILLA (Manilkara zapota (L.) van Royen) Sari, Vega Kartika; Murti, Rudi Hari
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Perennial crop leaves contain polysaccharides, polyphenols, and other secondary metabolites in high concentration. The presence of those compounds inhibit enzymatic activities and amplification of DNA. The existing extraction methods were not able to dissociate the metabolites contaminants of sapodilla (Manilkara zapota (L.) van Royen) leaves and thus resulting in low quality of extracted DNA. The aim of this experiment was to develop an effective method to extract DNA from mature leaf samples of sapodilla (Manilkara zapota (L.) van Royen). Fifth modification of Doyle & Doyle DNA extraction protocol with modified concentration of buffer reagent (consisted of: CTAB 2.8%; NaCl 2.5M; mercaptoethanol 3%, and PVP 2.5%) and repetition of some phase purification (liquid nitrogen; three times CIAA; two times ethanol 70%, RNAse 1µl) generated high quality DNA and clear band of PCR amplification using RAPD primers.   Keywords: buffer modifications, DNA extraction, sapodilla
Co-Authors Achmad Haikal Baswedan Adib , Affan Rizwanda Aditya Wahyu Pratama Ahmad Ilham Tanzil Aji, Galih Setiyo Al Fiqriansyah, Lilo Amalia, Dita Ambarwati, Septiani Tri Andini Ike B. Pramudita Andreano, Rio Tito Anggriawan, Rendy Ardelia Argiyanti Basuki Basuki , Basuki Basuki BASUKI BASUKI Basuki, Basuki Bisyri , Ahmad Janukhi Chalen Vito Nanda Choiriyah, Nikmatul Christy, Rullita Tio Damanhuri Damanhuri Deana Nathania Damayanti Destiawan, Hisyam Ashar Destiawan, Hisyam Azhar Dewi Shinta Kumalasari Dok Sakinah Dwi Amalianingtias Dwi Nurahmanto Dwi Nurahmanto Dyah Ayu Fitriani Elfrida Ratnawati Eliyatiningsih, Eliyatiningsih Eva Rosdiana Farisi, Oria Alit Fatah, Muhammad Rizal Fatya, Adrin Putri Furqon, Muhammad Ariful Gita Permata Sari Grandys Prameswari Halimatus Sa'diyah Hasan Fikri, Hasan Himmatul Khasanah Hutauruk, Laura Ro Vega Br. Ika Bella Aprillia Pasaribu Indrajid, Sukma Maulana Isnanto, Bimo Arvi Aji Jannah, Shelsa Novita Na'immatul Juliansyach, Naufal Kacung Hariyono Kaisardi, Rekzy Pradwipta Karomah, Amanda Roudatul Kessumowati, Dyah Ayu Kesumowati, Dyah Ayu Khairur Rahman Ilyas Khofi, Achmad Chaqiqotul Khomariah, Feby Dea Nur Laily Mutmainnah Listya Purnamasari Luluk Noviana M. Zayin Sukri Maharani, Sherly Apta Mandala, Marga Marga Mandala Maulana, Muhammad Irfan Mochamad Alfandi Risfansyah Mochammad Fahrur Rozi Mohammad Nur Khozin Mohammad Rayhan Maulana Muhammad Ferdiansyah Muhammad Ridwan Hadiyanto Nabilah Dwi ‘Ulayya Ambaroh Naura, Lintang Arsa Nazmi Kamilla Putri Neta Aurina Widyadhani Nikmatul Choiriyah Nur Fatimah Nurul Dwi Novikarumsari, Nurul Dwi Oria Alit Farisi Oria Alit Farisi Pertami, Rindha Rentina Darah Pitaloka, Amelia Ayu Prasetya, Eka Bagus Budi Prastiyo, Hendrik Eka Rahayu, Yeni Dwi Ratih Apri Utami Ratih Apri Utami Refa Firgiyanto Refa Firgiyanto Restanto, Didik Pudji Rindha Rentina Darah Pertami Ristiyana, Suci Rohman, Fahmi Arief Rudi Hari Murti Rusdiana, Riza Yuli Sa'diyah, Halimatus Sabila, Revina Putri Sabilillah, Vanya Yuni Artafirdaus Safitri, Erna Indah Dwi Safitri, Urmita Dwi Saputra, Yongky Purna Sari, Ani Dwita Sa’diyah, Halimatus Septian Yoga Pratama Setiawati, Tri Candra Setyo Andi Nugroho Shafira, Alivia Nur Siswadi, Edi Siti Aisyah Siti Ma'rufah Sri Hartatik Sri Rahayu Steven, Syahrul Suci Ristiyana Sukri, M. Zayin Sukron Romadhona Suryono, M. Iqbal Krisna Hadi Susanto, Devani Avrillia Tri Rini Kusparwanti Tri Rini Kusparwanti, Tri Rini Tri Wahyu Saputra Umroh, Windatul Valentina Tasya Angelina Widianingrum, Desy Cahya Widya Kristiyanti Putri Windari, Riski Ayu Yulianto, Roni