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Callus Induction of Dendrobium discolor Through The Thin Cell Layer (TCL) Technique Added with 2,4-Dichlorophenoxyaceticacid Iga Permata Hany; Zozy Aneloi Noli; M. Idris
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4b.5808

Abstract

Thin Cell Layer (TCL) is a plant propagation technique using thin explants (1-2 mm) through tissue culture. Callus induction is a crucial step in establishing plant regeneration. The type and concentration of growth regulators influence callus formation. 2.4 D is a growth regulator that is commonly used for callus induction in various plants. The research aims to determine the effectiveness of thin explants and 2,4-D in inducing Dendrobium discolor callus. The method used a Completely Randomized Design (CRD) with four treatments (1, 2, 3, and 4 mg/L). Adding 1, 2, and 3 mg/L 2,4-D can induce 100% calluses through the TCL explant. The callus has a friable to compact texture. The result showed that 2 mg/L 2,4-D was the best concentration for callus induction of D. discolor through TCL technique, indicating the greenish color, friable texture, and meristematic tissue.
Application of Portulaca oleracea L. Extract as a Biostimulant with Several Types of Solvents on The Growth of Kale (Brassica oleracea var. achepala) Cleopatra; Zozy Aneloi Noli; M. Idris; Chairul; Suwirmen; Solfiyeni
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5711

Abstract

Plant extracts are one biostimulant sources that have the potential to increase plant growth and yield. Portulaca contains secondary metabolites, macro and micronutrients, and amino acids, which have the potential as biostimulants. The research aims to determine the best type of solvent in the Portulaca extraction process as a biostimulant. The method used in this study was a Completely Randomized Design (CRD) with three treatments (aquadest, ethanol, and methanol) and nine replicates. The application of Portulaca extract extracted with several solvents significantly affected root length parameters of kale, with the most extended the root length of 15.80 cm. However, it did not have a significantly different effect on other parameters. Based on the proximate analysis, Portulaca extract extracted with ethanol solvent produces a protein content of 25.26%.
Preliminari Eksperimen dalam Penginduksian Akar Stek Tumbuhan Tetrastigma papillosum Inang Rhizanthes deceptor Chairul Chairul; Rizka Sefmaliza; M. Idris
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9045

Abstract

Tetrastigma is a genus in the Vitaceae family which is characterized by the presence of tendrils. This plant has a liana habit, which requires tall plants as support in its life. Induction of host roots, Tetrastigma papillosum, in this study, used Naphthalene Acetic Acid (NAA), which is a class of auxin that functions to induce cell expansion and initiation of rooting. This research was conducted using a Completely Randomized Design (CRD) with 5 treatments and 5 replications. Several NAA concentrations as treatments consist of A (0 ppm), B (2.5 ppm), C (5 ppm), D (7.5 ppm), and E (10 ppm). Root induction of Tetrastigma papillosum can be initiated with NAA at small concentrations because endogenous auxin induces roots more quickly.
Perancangan Desain Kemasan Velva Buah Nipah dan Ubi Jalar Ungu Menggunakan Metode Quality Function Deployment (QFD) Juarsa, Rahmadini Payla; Hanif, M. Khairul; Qalbi, Raihanul; Aqil, Nazliana; Putra, Aidil Jaya; Idris, M.; Jumiati, Melvy; Bela, Aldi Okta
Teknotan: Jurnal Industri Teknologi Pertanian Vol 18, No 2 (2024): TEKNOTAN, Agustus 2024
Publisher : Fakultas Teknologi Industri Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jt.vol18n2.6

Abstract

Velva buah nipah dan ubi jalar ungu adalah produk sejenis es krim yang memiliki kadar lemak yang rendah karena menggunakan buah nipah dan ubi jalar ungu sebagai bahan utamanya. Desain kemasan yang tepat diperlukan untuk melindungi velva buah nipah dan ubi jalar ungu serta agar terlihat menarik. Tujuan dari penelitian ini adalah untuk menghasilkan desain kemasan velva buah nipah dan ubi jalar ungu yang sesuai dengan keinginan konsumen. Penelitian ini menggunakan metode Quality Function Deployment yang mengakomodir voice of customer dalam pembuatan desain kemasan velva buah nipah dan ubi jalar ungu. Hasil penelitian menunjukkan bahwa terdapat dua alternatif desain kemasan velva buah nipah dan ubi jalar ungu yang dihasilkan. Alternatif kemasan berbentuk cup, memiliki desain visual yang memuat ilustrasi produk, nama produk, dan nama produsen, terdapat nomor BPOM, logo halal, dan tanggal kadaluarsa serta warna dasar kemasan yang berwarna cerah (merah muda) dan gelap (hijau).
Pengujian Limbah Air Tahu Terhadap Jumlah Stomata dan Kandungan Klorofil Tanaman Kedelai Hitam (Glycine soja L. ) Idris, M.; Rahmadina, Rahmadina
JURNAL AGROPLASMA Vol 9, No 1 (2022): AGROPLASMA VOL 9 NO 1
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v9i1.2660

Abstract

The purpose of this study become to determine the impact of giving tofu waste water on the content material of chlorophyll and stomata in black soybean (Glycine soja L.). The studies became conducted from August to October 2021 at Pantai Labu. Jl. Burhanuddin Dusun 1 Deli Serdang Regency. Analysis of chlorophyll content material and stomata at Plant physiology Laboratory of USU. The substances use have been black soybean (Glycin soja L.), EM4 550 ml, 5 liter tofu waste water, 200 ml molasses, 400 gr brown sugar. The tools used are paranet, 20 liter water drier, measuring cup, filter, polybag, soil, ruler, pen, label paper, meter and pH indicator. Variabelobservation  have been the number of stomata, chlorophyll content measured at the end of the generative phase at 30 days after planting. Calculation of the number of stomata changed into performed the use of a photomicograph the usage of freshly sliced preparations at the suggestions of the leaf shoots, whilst the analysis of chlorophyll content material became accomplished the usage of acetone solvent.This study used non-factorial randomized block design (RCBD). The treatment is Tofu Water Waste (K) at four stage, K0 = 0%, K1 = 15%, K2 = 20%, K3 = 25% with 3 times replication. The analysis data usedStatistical Product and Service Solutions (SPSS) version 20 on the one way ANOVA test with a significant level of 5% and continued with the Duncan Multiple Range Test  (DMRT). The results showed that the provision of tofu waste water had no significant effect at the number of stomata and the amount of exact chlorophyll determined in leaf sampling in multiples of three (young leaf), 5 (middle leaf), and 8 (old leaf) from the top of leaf tip. Visually, the pleasant treatment for the number of stomata in K3 (25% concentration of tofu water waste) via taking leaf samples in multiples of 5 (young leaf) from the tip of the leaf shoot. meanwhile, the amount of chlorophyll in K1 is tofu water waste at a concentration of 15% through taking leaf samples in multiples of 8 (old leaf) from the end of the leaf shoot.Keywords: wastewater tofu, number of stomata, chlorophyll content, black soybean
The Effect of Gamal Leaf (Gliricidia sepium (Jacq.) Kunth ex Walp)-based Liquid Organic Fertilizer on The Vegetative Growth of Lettuce (Lactuca sativa L.) Nisa, Chairul; Idris, M; Rahmadina
SCISCITATIO Vol. 5 No. 1 (2024): Volume 5 Number 1, January 2024
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2024.51.164

Abstract

The growth of lettuce depends on the interaction of growth and environmental conditions. Improper crop maintenance may cause low yield of lettuce production. Application of liquid organic fertilizer could be performed as a strategy in crop maintenance. This study aimed to determine the effect and concentration of gamal leaf (Gliricidia sepium (Jacq.) Kunth ex Walp)-based liquid organic fertilizer (LOF) on the vegetative growth of green lettuce plants (Lactuca sativa L.). The research method used a non-factorial Randomised Group Design (RGD), with treatments consisted of P0 (control), P1 (20% gamal leaves-based LOF), P2 (40% gamal leaves-based LOF), and P3 (60% gamal leaves-based LOF). The results showed that Gamal leaves-based liquid organic fertilizer (LOF) that is produced in this research might still contain less macronutrients C, N, P and K that is stated by SNI 7763: 2018 (2-6%), but its application on lettuce as tested plants could still support their growth. Dose of P1 (20%) was the best to support lettuce growth in the form of increased plant height and leaf area index. Dose of P2 (40%) was abble to maintain showed minimum decrease on lettuce total chlorophylls content. Application of dose of P2 (40%) on lettuce growth medium supportes highest uptake of N while the application of dose of P3 (60%) showed highest uptake of P.
PENGARUH MEDIA TANAM TERHADAP PERTUMBUHAN VEGETATIF SELADA MERAH (Lactuca sativa var. crispa) YANG DIBERI AB MIX PADA SISTEM HIDROPONIK SUMBU (WICK SYSTEM Simanjuntak, Putri Dea Firsta; Rahmadina, Rahmadina; Idris, M
Jurnal Biogenerasi Vol. 9 No. 1 (2024): Terbit volume 9 nomor 1 tahun 2024
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v9i1.3651

Abstract

Selada merah (Lactuca sativa var. crispa) merupakan tumbuhan vegetatif dengan kandungan antosianin sebagai sumber antioksidan dan vitamin. Penggunaan hidroponik sumbu (wick system) sebagai alternatif budidaya selada merah ditentukan oleh jenis media asal pembenihan dan konsentrasi nutrisi AB Mix yang digunakan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian jenis media dan pengaruh konsentrasi AB Mix terbaik serta interaksi kombinasi perlakuan jenis media dan konsentrasi nutrisi terhadap pertumbuhan vegetatif selada merah. Metode yang digunakan ialah sistem hidroponik sumbu. Parameter pada penelitian ini adalah tinggi tanaman, jumlah daun, luas daun, kadar klorofil. Hasil penelitian menunjukkan bahwa perlakuan jenis media dan perlakuan konsentrasi nutrisi AB Mix berpengaruh terhadap seluruh parameter pengamatan. Konsentrasi terbaik adalah 1200 ppm AB Mix, sedangkan jenis media terbaik ialah rockwool. Kombinasi perlakuan media tanam rockwool dan konsentrasi AB Mix 1200 ppm menunjukkan kombinasi perlakuan terbaik pada parameter tinggi tanaman (10,93 cm), jumlah daun (11,00), luas daun (5,07 cm2), dan kadar klorofil (6,39). Kata Kunci: Hidroponik, Konsentrasi, Selada Merah
Pengaruh Pemberian Pupuk Organik Cair Dari Kombinasi Limbah Bonggol Pisang Dan Rebung Bambu Terhadap Pertumbuhan Tanaman Sawi Pagoda (Brassica narinosa L.) Secara Hidroponik Sumbu (Wick System) Mawarni, Devi; Rahmadina, Rahmadina; Idris, M
Jurnal Biogenerasi Vol. 9 No. 1 (2024): Terbit volume 9 nomor 1 tahun 2024
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v9i1.3672

Abstract

Sawi pagoda adalah jenis tanaman yang berasal dari tiongkok yang lebih dikenal dengan nama lain ta ke chai dan merupakan salah satu jenis tanaman sawi yang mengandung banyak nutrisi, serat, vitamin, mneral dan antioksidan yang berfungsi sebagai pencegah kanker. Salah satu upaya meningkatkan pertumbuhan sawi pagoda yaitu dengan cara hidroponik dan pemberian pupuk. Penelitian ini dilakukan pada bulan juli-oktober 2023. Metode penelitian ini menggunakan Rancangan Acak Kelompok (RAK) nonfaktorial yang terdiri dari satu factor dengan tiga kali ulangan. Faktor yang berpengaruh yaitu pupuk organic air yang terdiri dari 3 konsentrasi (100 ml, 200 ml dan 300 ml). Parameter pengamatan pada penelitian ini meliputi tinggi tanaman, jumlah daun, berat basah dan luas daun. Hasil penelitian ini menunjukan bahwa pemberian pupuk organik cair dari kombinasi limbah bonggol pisang dan rebung bambu berpengaruh signifikat terhadap pertumbuhan tanaman sawi pagoda (Brassica narinosa L.) dengan parameter tinggi tanaman, jumlah daun, berat basah dan luas daun. . Konsentrasi pupuk organik cair terbaik adalah 200 ml. Kata Kunci: Hidroponik, Sawi pagoda, Pupuk organik cair
APLIKASI PUPUK ECO FARMING DAN PENAMBAHAN GIBERELIN TERHADAP PERTUMBUHAN BAYAM MERAH (Amaranthus tricolor L.) PADA SISTEM WALL PLANTER BAG Syafira, Zara; Idris, M.; Rahmadina
Jurnal Biogenerasi Vol. 9 No. 2 (2024): Volume 9 Nomor 2 tahun 2024 periode (Juni 2024 - September 2024)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v9i2.3821

Abstract

Based on the Central Statistics Agency (2022), red spinach production is said to be unstable at this time. The application of eco farming and gibberellins as well as utilizing the home environment to use a wall planting bag planting system is one strategy to overcome this problem. The aim of this research is to determine the correct dosage for the growth of red spinach plants planted using a wall planter bag system. This research was conducted in September-October 2023. This research used a factorial randomized block design, namely E0 (control), E1 (8 ml/l), E2 (16 ml/l) and E3 (24 ml/l) with G0 (control , G1 (200 ppm) and G2 (250 ppm). Based on the observations made, the combination treatment had a significant effect on plant height and leaf area, but had no effect on plant fresh weight. The E3G2 treatment was the most effective dose in this study.
PROPAGASI IN VITRO KALIANDRA MERAH (Calliandra calothyrsus Meisn.) I: PERKECAMBAHAN BIJI DAN INISIASI TUNAS DARI EKSPLAN HIPOKOTIL DAN NODUS Idris, Muhammad; Asman, Auzia; Sorel, Deni; Joniarti, Edi; Mohtar, Ulfa; Harmailis, Harmailis; Nefri, John; Salivia, Salivia
Bio-Lectura : Jurnal Pendidikan Biologi Vol. 11 No. 1 (2024)
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/bl.v11i1.16877

Abstract

The red calliandra is a fuel wood crops that gave benefit for people. Recently, it is getting wide attention due to its important use as biofuel in industrial scale. The massive propagation is needed to meet industrial needs. Beside the use of conventional seedling production technique, the use of tissue culture for mass propagation is one of the alternatives to address this problem. This research was conducted to develop standard protocol for micropropagation of red calliandra through tissue culture. Here, the first step for that purpose was reported. The MS medium was used for basal media treatment. The seed source was stored for eight months under 5oC. This research was carried out in two stages i.e. 1) seed germination test and 2) shoot initiation and multiplication treatment. Each stage was arranged in experimental method. The result showed that seed were pre-treated with warm water (40oC) gave 62.5% germination under red light when compared to control (37.5%, pre-treated with water at 25oC under white light) three days after planting. Conversely, there is no striking differences of germinated seed percentages between light treatment at 25oC. Furthermore, it was found that addition of 2 mg/L BAP gave better result in shoot initiation and number of shoots formed from nodal explants after four weeks in treatment media. These results indicated that mass propagation of red calliandra can be done through tissue culture where multiplication stage is one of the important steps to produce multiple shoots only from single nodal explant.
Co-Authors . Mansyurdin Anita Ariani Anwar Fuadi, Anwar Anzharni Fajrina Apriani, Dwi Wahyuni Aprilia, Tiara Aqil, Nazliana Asif, M. Asman, Auzia Audina, Nida Auliasari, Putry Ayu Auzia Asman Bela, Aldi Okta Chairul Chairul Chairul Chaudhary, M. Cleopatra Deni Sorel Dola Ratna Yulizar Dwi Anggraini Edi Joniarti Eli Ratni Ermalianti, Ermalianti Erna Astuti Erni Febriyanti Eron Swandra Faisal, Agung Fanadillah, Yurike Farooq, U. Febriani, Annisa Vada Fitriah, Wani Gita Prima Yudha Hafizah, Ummi Hameed, R. Hanif, M. Khairul Hany, Iga Permata Harmailis Harmailis Harmailis, Harmailis Hasibuan, Rahmayani Iga Permata Hany Irda Nila Selvia Jelita Putri Adisti John Nefri Joniarti, Edi Jumiati, Melvy Kurnia, Meri Lashari, M. H. Lendrawati Lubis, Habib Jailani Lubis, Wisuda Pramarta Mardiana Puspasari Marta Linda Martomo Setyawan Mawarni, Devi Mohtar, Ulfa Muhammad Hanafi Murdifin Natalia, Ferli Nefri, John Nelvia, Repi Netty W Surya Netty W Surya Nisa, Chairul Pratama, Yuda Rizky Putra, Aidil Jaya Putri, Shintiya Rahmadhani Qalbi, Raihanul Rahmadina Rahmadina, Rahmadina Rahmadini Payla Juarsa Rehman, Z. U. Rhomadoni, Firda Rizki Ridha Permata Sari Rizka Sefmaliza Salivia, Salivia Salvia Salvia Santhyami Santhyami Sari, Leli Mustika Sefmaliza, Rizka Simanjuntak, Putri Dea Firsta Siregar, Nuri Jannah Tussadiyah Siregar, Rivahni Siti Jamilatun Situmorang, Vickasyah Ramadani Solfiyeni Solfiyeni Sorel, Deni Sundari, Lia Suwirmen, Suwirmen Syafira, Haiyu Asy Syafira, Zara Tita Nopiyanti Totok Eka Suharto Ulfa Mohtar Vebianti, Rani Wandini, Dwi Febiyanti Wardhana, Budi Setya Wardhana1, Budi Setya Yamaly, Fadhil Yulianti, Sisi Zahratul Idami Zahro, Adinda Husna Fatia Zahrul Mufrodi, Zahrul Zozy Aneloi Noli