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Kurniawan Kurniawan
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Journal : Masker Medika

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PENGARUH DIMETIL SULFOKSIDA (DMSO) DAN SUHU PRESERVASI BERBEDA TERHADAP BAKTERI Methicillin-Resistant Staphylococcus aureus (MRSA) Kurniawan Kurniawan
Masker Medika Vol 11 No 2 (2023): Masker Medika
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/maskermedika.v11i2.563

Abstract

Introduction: S. aureus bacteria are bacteria that cause nosocomial infections in hospitals and that easily develop resistance to antibiotics, forming new strains such as MRSA bacteria that are resistant to the class of antibiotics methicillin (β-lactams). MRSA bacteria are pathogenic and carry a significant proportion of hazards, so it is necessary to maintain and store pure cultures of MRSA bacteria in the laboratory for the purposes of exploration and efforts to control and treat infectious diseases caused by these bacteria. One of the solutions used in bacterial preservation is dimethyl sulfoxide (DMSO) solution, which is universally used. This study aimed to determine the effect of DMSO solution and different temperatures on the preservation of MRSA bacteria. Methods: This study was a quantitative study using multivariate ANOVA test analysis conducted at the Microbiology and Biochemistry Laboratory, Biology Education Study Program, Teaching and Education Faculty (FKIP), Muhammadiyah University, Purwokerto, from July 2022 to October 2022. Results: DMSO solution concentration and temperature Different preservatives can affect the viability of MRSA bacteria, while the best temperature for preserving MRSA bacteria is between 4 oC and 20-28 oC with a significance value (p=0.048). Discussion: The research results show that the concentration of DMSO solution and different preservation temperatures can affect the viability of MRSA bacteria. The best temperature preservation was obtained at 4 oC. Keywords: Bacterial preservation, DMSO, MRSA, Temperature
Co-Authors Abd Rahman Rusydi Hasibuan Achyani Achyani, Achyani Adibrata, Sudirman Agrina Agrina Agung Vega Andreas Ahmad Amikmiami Ahmad Gilang Barokat Ahmad Zaenul Islam Ai Nur Solihat Alias Pikal Alzetrho Baja Pratama Anand Satrio Muhamad Andi Gustomi Aneng Yuningsih Anggraeni Yunita Anung Ahadi Pradana Arif Febrianto Arthur Brown Asep Setiadi Astri Srigustini Ati Sadiah Bahtiar Sah Putra Bustari Bustari Casman, Casman Christianingrum Dedi Afandi Dela Amelia Damayanti Dela Ristiani Deni Radona Denisa Nopita Devi Anatasya Dimas Dwi Prasetyo Ditya Nurul Fauziyah dody hapsoro Dwi Risma Deviyanti Dyah Melita Febryana Ayuningtyas Edwin Edwin Ela Anisa Elvina Elvina Endang Masitoh Eriyono Budi Wijoyo Farhaby, Arthur M Fauziah Azmi Feni Marlinda Feri Candra Ghina Dhaniyah Salsabil Gugum Gumilar Habib Habib Hadinata, Novri Hafizhah Khairana Hanafi Hanafi Harry Azhar Azis Hendri Hermawan Adinugraha Hening Widowati Hirsanuddin Hirsanuddin Iis Fauziah Ikmawati Ikmawati Indah Denissa Lestyaninrum Indrawari, Karliana Intan Ristri Alkhila Iphov K. Sriwana Irman Firmansyah Isti Halimah Rosanti Joel Aubin Junaidi, Riskan Kensya Selvin Natalia Khoirul Amrillah KHOIRUNNISA PASARIBU Kiki Nurjanah Kurnia Ritma Dhanti Lanang Satria Wiralaga Lilik Sabdaningtyas Lyna Raharjo M Bachtiyar M Yusuf M. Yogi Riyantama Isjoni M.Rizza Muftiadi Magdalena Nany Maretha Angelina Sianturi Melani Novitasari Muh. Saleh Nurdin Muhammad Al Kautsar Muhammad Haritza Laitte Mustajada, Nafaisul Nandika Alfan Firdaus Nandika Alfan Firdaus Natal Indra Nur Hakima Akhirani Nur Vira Natasya Nutria Permadani Oden Krisyan R. Neneng Rina Andriani Raden Roro Suci Nurdianti Rani Rahman Ranti Rahmawati Nurhalizah Rayimas Priti Aisyapuri Reisa Evita Zahradia Rendi Rendi Rendra Gumilar Reni Reni Retno Sulistiyowati Retno Sulistiyowati Reza Okva Marwendi Rezya Marsanda Aprilia Riana Rachmawati Dewi Rida Munggarani Rindiana Mariana Rio Pebrianto Riris Yuli Valentine Riza Safira Rizki Azhar Nur Farid Rosa Nikmatul Fajri Rukmini, Reni Dianti Septia Nurahmawati Siti Aisyah Siti Nur Maulidah Srihadi Winarningsih Suarman Suarman Suri Purnama Febri Suwarjo Suwarjo Syahna Utami Nisa Syamsiar, Syamsiar Teguh Ferdinand Teuku Fadlon Haser Tri Heru Prihadi Ulfi Tiya Fauziyah Umroh Umroh Vaul Vaul Wahyu Adi Winda Sari Yana Priyana Yanuar Ilham Yanuar Yanuar Yeyen Mardyani Yusril Ikhsanuddien Yusuf Iskandar Zahra Fazhira Zahrah Nazhifah Iftinany