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All Journal Jambi Medical Journal : Jurnal Kedokteran dan Kesehatan International Journal of Renewable Energy Development Journal of Nutrition College Jurnal Pendidikan Jasmani El-Hayah : Jurnal Biologi Jurnal Teknik Kimia The Journal of Pure and Applied Chemistry Research Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) MNJ (Malang Neurology Journal) EMPATI : JURNAL ILMU KESEJAHTERAAN SOSIAL Jurnal Ilmiah Pendidikan Pancasila dan Kewarganegaraan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) JIKA (Jurnal Informatika) Sains dan Matematika J-PEN Borneo : Jurnal Ilmu Pertanian Indonesian Journal of Law and Economics Review Indonesian Journal of Applied Research (IJAR) MEDIC MEdical Dedication JURNAL MANAJEMEN AKUNTANSI (JUMSI) Jurnal Ilmu Sosial Indonesia (JISI) Journal Peqguruang: Conference Series Jurnal Tadbir Peradaban Postgraduate Management Journal TAMIKA: Jurnal Tugas Akhir Manajemen Informatika & Komputerisasi Akuntansi Jurnal Sains Alami (Known Nature) Majapahit Journal of Islamic Finance dan Management Jurnal Keuangan dan Perbankan Syariah El-Mal: Jurnal Kajian Ekonomi & Bisnis Islam Politeia : Journal of Public Administration and Political Science and International Relations Journal of Ekonomics, Finance, and Management Studies Jurnal Pengabdian dan Inovasi Masyarakat
ahmad syauqi, ahmad
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Journal : Sains dan Matematika

 1 
ISOLASI DAN KARAKTERISASI BACILLUS SP. PELARUT FOSFAT DARI RHIZOSFER TANAMAN LEGUMINOSAE Mukamto, Mukamto; Ulfa, Syazwani; Mahalina, Weda; Syauqi, Ahmad; Istiqfaroh, Laila; Trimulyono, Guntur
Sains & Matematika Vol 3, No 2 (2015): April, Sains & Matematika
Publisher : Sains & Matematika

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Abstract

Rhizosfer tanaman Leguminosae banyak ditumbuhi bakteri, salah satunya adalah genus Bacillus yang merupakan Plant Growth Promoting Bacteria yang bermanfaat bagi tumbuhan, di antaranya karena kemampuannya dalam melarutkan fosfat. Tujuan penelitian ini untuk mendapatkan isolat Bacillus sp. dari rhizosfer tanaman Leguminosae yang memiliki kemampuan melarutkan fosfat. Penelitian ini dilakukan dengan melakukan isolasi bakteri, karakterisasi koloni, pewarnaan Gram, pewarnaan endospora, uji katalase, dan uji kelarutan fosfat. Metode isolasi yang digunakan dengan metode pour plate dan untuk pengujian bakteri pelarut fosfat menggunakan media Pikovskaya. Sebanyak 36 isolat Bacillus sp. berhasil diisolasi dengan berbagai karakter koloni dan karakter sel berbentuk batang, Gram positif, membentuk endospora, dan memiliki katalase positif kecuali isolat BAP2 dan BAP5 yang memiliki katalase negatif. Sebanyak lima isolat Bacillus sp. (isolast BCP 2, BCP 4, BAC 2, BCM 3, dan BAP 1) diketahui mampu melarutkan fosfat dengan terbentuknya holozone di sekitar koloni setelah masa inkubasi tujuh hari. Rhizosphere of Leguminosae is inhabited by bacteria, one of which is the genus Bacillus which is Plant Growth Promoting Bacteria that are benefi cial to plants, such as its ability to dissolve phosphate. The purpose of this study was to obtain isolates of Bacillus sp. from the rhizosphere of Leguminosae plants which has the ability to dissolve phosphate. This study was conducted by bacterial isolation, characterization colonies, Gram staining, coloring endospores, catalase test, and solubility test phosphate. Isolation methods used by the pour plate method for testing bacteria and phosphate solvent using Pikovskaya media. A total of 36 isolates of Bacillus sp. successfully isolated colonies with different characters and character of rod-shaped cells, Gram-positive, form endospores, and has a positive catalase except isolates BAP2 and BAP5 which has a negative catalase. A total of fi ve isolates of Bacillus sp. (Isolast BCP 2, BCP 4, BAC 2, 3 BCM, and BAP 1) known to be capable of dissolving phosphate holozone formation around the colonies after an incubation period of seven days.
Isolasi dan Karakterisasi Bacillus sp. Pelarut Fosfat dari Rhizosfer Tanaman Leguminosae Mukamto, Mukamto; Ulfa, Syazwani; Mahalina, Weda; Syauqi, Ahmad; Istiqfaroh, Laila; Trimulyono, Guntur
Sains & Matematika Vol 3, No 2 (2015): April, Sains & Matematika
Publisher : Sains & Matematika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Rhizosfer tanaman Leguminosae banyak ditumbuhi bakteri, salah satunya adalah genus Bacillus yang merupakan Plant Growth Promoting Bacteria yang bermanfaat bagi tumbuhan, di antaranya karena kemampuannya dalam melarutkan fosfat. Tujuan penelitian ini untuk mendapatkan isolat Bacillus sp. dari rhizosfer tanaman Leguminosae yang memiliki kemampuan melarutkan fosfat. Penelitian ini dilakukan dengan melakukan isolasi bakteri, karakterisasi koloni, pewarnaan Gram, pewarnaan endospora, uji katalase, dan uji kelarutan fosfat. Metode isolasi yang digunakan dengan metode pour plate dan untuk pengujian bakteri pelarut fosfat menggunakan media Pikovskaya. Sebanyak 36 isolat Bacillus sp. berhasil diisolasi dengan berbagai karakter koloni dan karakter sel berbentuk batang, Gram positif, membentuk endospora, dan memiliki katalase positif kecuali isolat BAP2 dan BAP5 yang memiliki katalase negatif. Sebanyak lima isolat Bacillus sp. (isolast BCP 2, BCP 4, BAC 2, BCM 3, dan BAP 1) diketahui mampu melarutkan fosfat dengan terbentuknya holozone di sekitar koloni setelah masa inkubasi tujuh hari. Rhizosphere of Leguminosae is inhabited by bacteria, one of which is the genus Bacillus which is Plant Growth Promoting Bacteria that are benefi cial to plants, such as its ability to dissolve phosphate. The purpose of this study was to obtain isolates of Bacillus sp. from the rhizosphere of Leguminosae plants which has the ability to dissolve phosphate. This study was conducted by bacterial isolation, characterization colonies, Gram staining, coloring endospores, catalase test, and solubility test phosphate. Isolation methods used by the pour plate method for testing bacteria and phosphate solvent using Pikovskaya media. A total of 36 isolates of Bacillus sp. successfully isolated colonies with different characters and character of rod-shaped cells, Gram-positive, form endospores, and has a positive catalase except isolates BAP2 and BAP5 which has a negative catalase. A total of fi ve isolates of Bacillus sp. (Isolast BCP 2, BCP 4, BAC 2, 3 BCM, and BAP 1) known to be capable of dissolving phosphate holozone formation around the colonies after an incubation period of seven days.
Co-Authors Abdul Khalik Agustin, Sekar Sari Aisyah Wardani, Aisyah Ali Djamhuri Allinha Yusfin Innaya Amaliyah, Viki Andaru Dahesihdewi Andrianto, Fathinah Ulfah Anggraeni, Agintha Silvya Anshori, Syaiful Apriliani, Susi Arhabi, Abuddafi Arifin Fauzi Lubis Armaidi Darmawan Atika Atika Awaludin, Dipa Teruna Awan, Edi Aziz, Achmad Qomarul Baskara, Vanza Aulia Belqis, Farah Nabila Choiroh, Durrotul Darizal, Adjeng Amelia Lucky Delfira, Annisa Erny Kusdiyah Fairuz Quzwain Faisal Fakhri, Ayushia Generosa Fakhri, Sarah Humayra Fatmawati Fatmawati Fuadi, Muhammad Chaniful Fuadi, Mukhlisul Guntur Trimulyono Hamdy, M. Kholis hani septiana, hani Haq, Fafa Maulal Hari Santosa, Hari Hari Santoso Hartanti Sandi Wijayanti Hasan Zayadi Hasmita, Debby Hidayati, Arini Hotimah, Sitti Norul Irzan Soepriyadi Iskandar Yusuf Istiqfaroh, Laila Iwan Permadi Juliyanti, Afifah Koto, Rinta Febrina Kusmawati, Eka Kusumawardhany, Intan Ratna Lailah, Rukhil Lathifah, Iffah Maghfirah, Inayatul MAHALINA, WEDA Majida Ramadhan Maulidiyah, Ni'matul Melinda, Fatihatul Mubarok, Imam Anas Mufidah, Ana Liatul MUHAMMAD BAKRI MUKAMTO MUKAMTO, MUKAMTO Muryanah, Siti Muzayyanah, Nana Ningrum, Mei Ninda Nisa, Siti Rofiatun Nur Fazat Arinal Haq, Nur Fazat Arinal NUR KHOLIFAH Nurdin, Immega Adelia Nurmala, Aulia Putri Nyimas Natasha Ayu Shafira Pakiding, Mainar Daud Puspithasari, Agustina Dwi Rachmawati, Rachmawati Suprayoto Ramadhan, Majida Ranitadewi, Ika Nindyas Ratna Djuniwati Lisminingsih Refiandinova, Fatur Rini Oktari Batubara Riyadi, Tomi Rodiyanto Rohimah, Ririn Alfiatu Rohmatillahil Jamilah, Favi Rustandi, Helmi Saada, Iqlimatus Saifudin Asrori Saimul Laili Samsirun Halim Setyawan, Febrianto Sholehuddin, Mohammad Siti Fatimah Siti Fatonah Sonny, Sonny Sotianingsih, Sotianingsih Sri Wahyuni Suhartini, Venita Sukiran, Nufan Muwafiq Suud Ema Fauziah Syifa, Carla Amadea Tarigan, Miska Irani Tintrim Rahayu, Tintrim Tresna, Adira Tatus Trisna, Adira Tatus Ulfa, Syazwani Umaroh, Sofiyatul Umma, Firda Firdausi Utami, Ade Ratna Varadibbah, Hasina Wahyu Indah Dewi Aurora Wakik, Wakik Widodo, Laurentius Urip Wijaya, Kusuma Lestari Wulandari, Rossa Martha Yuana, Hera Zaki, Siti Muzakiyah Zuliyana, Devy Zulkarnain, Rahmat