Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : PROSIDING SEMINAR KIMIA

THE POTENCY OF PROTEIN EXTRACTS FROMCandida albicans AS BIORECEPTOR ON IMMUNOSENSOR FOR DIAGNOSIS OF CANDIDIASIS Masfufatun -; Noer Kumala; Afaf Baktir
PROSIDING SEMINAR KIMIA PROCEEDING OF INTERNATIONAL CONFERENCE 2015
Publisher : PROSIDING SEMINAR KIMIA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Currently diagnosis of candidiasis still usingthe traditional standard blood culture method. The traditional method were less sensitive andtime consuming. The purpose of this research were to develope the more sensitive immunosensor based method, and to examine the potency of C. albicans protein extract as bioreceptor to detect C. albicans and its biofilm in the blood of candidiasis patients.The research methods include: (1) preparation of digestive gland liquid of snail (Achatina fulica); (2) extraction of protein from C. albicans through enzimatis and mechanic methods and (3) analyzing the protein extract as bioreseptor through immunodot assay.The research results showed that the snail enzymes has protein content 1.35 mg/ml and specific activity 1.96 unit/mg. The snail enzyme hydrolyzed the cell wall of C. albicans with and without sonication, produced planktonic extracell protein extract (PEP) and biofilm extracell proteinextract (BEP), planktonic intracell protein extract (PIP), and biofilm intracell protein extract (BIP), with protein content 1.44; 1.29; 1.29 and 1.21 mg/ml respectively. The biofilm intracell protein (BIP) showed antigenic property toward antibody anti-Candida (positive control),giving red spot on imunodot assay. Immunodot assay can distinguish negative control serum (health man) and positive Candidiasis control by using antigen 1 g/l and 50 l serum.
Co-Authors Adi Candra, Adi AFAF BAKTIR akhmad sudibya, akhmad ALMAIDAH SAFITRI Amalia Nadiasari Amrulloh, Azmi Khalid Ananta Sandi Putra Ananta, Zahrah Aswa Andini Risma Ayunda Dewi Andriani, Chenny Aritania, Putu Oky Atik Sri Wulandari Ayly Soekanto Ayu Cahyani Noviana Budhi Setiawan Budhi Setiawan Budhi Setyawan Chenny A Wiyono Chenny Andriani Wiyono Dadik Raharjo Emilia Devi D.R, Emilia Devi Fardiansyah, Al Farizah El Husna Fehima Syachsiyat Alya Firdausi Nuzula Habibi, Miftahul Handy Arief HARMAN AGUSAPUTRA Harsono Harsono Haryanto, Loo Herliani, Olivia I Kadek Wawan Agus Wijaya I MADE DWIKY KARINA JAYA Inawati Inawati Inawati Inawati Indahasari, Noer Kumala Islam, Afif Fajrul Isnaeni, Mutia Jalal, Ismul JAMES HADIPUTRA SUNARPO Joeniarti, Elika Jonathan Kartika Ishartadiati Kuntaman Kuntaman Laili Shafarina Fauzan Laily, Aulia Nur Listyawati, Agusniar Furkani Loo Hariyanto Raharjo, Loo Hariyanto Lusiani Tjandra Meilya Putri Syahida Mentari, Andi Oktaviana Mohammad Ali Izzudin Muhammad Rozzan Prananda Narotama, Harya Ni Luh Narita Vijayanti Ni Made Putri Pradnyandari NI PUTU MANIK SURYANINGSIH Noer Kumala Noer Kumala NOER KUMALA Noer Kumala Indahsari, Noer Kumala NUGROHO EKO WIRAWAN BUDIANTO Nur Kumala Indahsari, Nur Kumala Nur Kumala, Nur Nurma Fitri Parawidnyaningsih, Putu Ayu Aryanda Pramita Laksitarahmi Isrianto Pratika Yuhyi Hernanda Putu Oky Ari Tania Qurraty’ain, Seryna Hasna Rachmawati Dwi Apriliyani Ratnasari, Diana Tri Rini Purbowati Rini Purbowati Riska Ammalia Putri Risnanda, Kadek Rio Setijowati, Eva Diah Soebandhi, Santirianingrum Sri Lestari Utami Sugiharto Suhartati Suhartati Suhartati Syahrefi Syahrefi TEOFILUS DANI PRASETYOADI Tikapa Muwawiyai Pigome Tri Juliati Tri Rahayuningsih Tri Ratnasari, Diana Utami, Sri Lestari Vini Anggraini Wahyuni Dyah Parmasari, Wahyuni Dyah Wibisono, Freegied Satriya Wibisono, Freshinta Jellia Yoga Yuniadi