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All Journal Jurnal Kedokteran Brawijaya Althea Medical Journal AGRIVITA, Journal of Agricultural Science Biology, Medicine, & Natural Product Chemistry PROSIDING SEMINAR KIMIA Jurnal Terapan Abdimas UNEJ e-Proceeding Berkala Kedokteran Jurnal Kimia Riset BIOMA Jurnal Ilmiah Kedokteran Wijaya Kusuma Jurnal Kedokteran dan Kesehatan The Indonesian Journal of Infectious Diseases Interdental Jurnal Kedokteran Gigi (IJKG) Medika Kartika : Jurnal Kedokteran dan Kesehatan Abdimas UNWAHAS : Jurnal Pengabdian Masyarakat UNWAHAS Jurnal Abdidas Jurnal Pengabdian Masyarakat: Humanity and Medicine Journal of Multidisciplinary Applied Natural Science Berkala Penelitian Hayati Hang Tuah Medical Journal Prosiding Seminar Nasional Kusuma Prosiding Seminar Nasional COSMIC Kedokteran

Masfufatun Masfufatun

Departemen Biokimia, Fakultas Kedokteran, Universitas Wijaya Kusuma Surabaya, Indonesia

Author-ID : 5503071

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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EFEKTIVITAS KOMBINASI EKSTRAK DAUN SIRIH MERAH (Piper Crocatum) DAN EKSTRAK BIJI ALPUKAT (Persea americana) DALAM MENGHAMBAT PERTUMBUHAN Candida albicans Vini Anggraini; Masfufatun Masfufatun
Jurnal Kimia Riset Vol. 2 No. 2 (2017): Desember
Publisher : Universitas Airlangga

Abstrak Candida albicans merupakan salah satu mikrooganisme penyebab infeksi kandidiasis. Daun sirih merah (Piper crocatum) dan biji alpukat (Persea americana) telah diteliti dapat menghambat pertumbuhan Candida albicans. Tujuan penelitian ini adalah untuk mengetahui efektivitas kombinasi daun sirih merah (Piper crocatum) dan ekstrak biji alpukat (Persea americana) dalam menghambat pertumbuhan Candida albicans. Penelitian bersifat eksperimental laboratorium (true experiment) dengan pendekatan post test control group design only. Daun sirih merah dan biji alpukat diekstraksi secara maserasi menggunakan pelarut etanol. Pada masing-masing ekstrak etanol dilakukan uji pendahuluan untuk mengetahui konsentrasi optimum ektrak dalam memnghambat pertumbuhan C. albicans. Daya Hambat pertumbuhan C. albicans diuji menggunakan metode difusi. Hasil penelitian menunjukkan bahwa ekstraks daun sirih merah dan biji alpukat masing-masing memiliki daya hambat terbesar pada konsentrasi 10%. Kombinasi ekstrak daun sirih merah dan ekstrak biji alpukat memiliki daya hambat yang signifikan terhadap pertumbuhan Candida albicans dibandingakan kontrol negatif, kontrol positif dan ekstrak daun sirih merah. Dengan demikian kombinasi ekstrak daun sirih dan biji aplukat etanol berpotensi sebagai antifungi dalam menghambat pertumbuhan C. albicans sehingga diharapkan bisa mengurangi prevalensi kandidiasis. Kata Kunci : daun sirih merah, biji alpukat, Candida albicans, Abstract Candida albicans is one of the microbialism that causes candidiasis infection. Red betel leaf (Piper crocatum) and avocado seed (Persea americana) have been studied to inhibit the growth of C. albicans. The purpose of this study was to determine the effectiveness of red betel leaf (Piper crocatum) and avocado seed extract (Persea americana) combination in inhibiting the growth of C. albicans. Research is experimental laboratory (true experiment) with post test approach control group design only. Red betel leaf and avocado seeds are extracted by maceration using ethanol solvent. In each ethanol extract, a preliminary test was performed to find out the optimum concentration of extract in inhibiting the growth of C. albicans. C. albicans growth retardant was tested using diffusion method. The results showed that the extract of red betel leaf and avocado seeds each had the greatest inhibitory concentration at 10%. The combination of red betel leaf extract and avocado seed extract have significant inhibitory effect on C. albicans growth compared to negative control, positive control and red betel leaf extract. Thus, the combination of betel leaf extract and ethanol seed has the potential as an antifungal in inhibiting the growth of C. albicans so it is expected to reduce the prevalence of candidiasis. Keywords: red betel leaf, avocado seed, Candida albicans,

Effects of Curcumin on Stability and Efficacy of Neem Leaves Extract as Botanical Insecticides Elika Joeniarti; Masfufatun Masfufatun; Noer Kumala Indahsari; Endang Noerhartati
AGRIVITA, Journal of Agricultural Science Vol 42, No 2 (2020)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v42i2.2651

Botanical insecticides are considered biodegradable, environmentally compatible, and less toxic to non-target organisms than synthetic insecticides. Unfortunately, they are not attractive to Indonesian farmers due to its instability against light, temperature, and microbes, as well as they, have short storage time. This research was intended to produce a distinctive botanical insecticide that is resilient against the light, microbes, and durable. The role of curcumin in the stability and efficacy of neem (Azadirachta indica) leaves extract as a botanical insecticide against soybean pod sucking bug, Riptortus linearis was evaluated in the laboratory. This research was conducted at the Organic Chemistry Laboratory of Chemistry Department, Faculty of Science and Technology Airlangga University and the Plant Protection Laboratory, Faculty of Agriculture University of Wijaya Kusuma Surabaya, Indonesia, from March to October 2016. The results explained that curcumin is no effect on increase the stability of neem leaves extract solution towards UV light irradiation. However, it can increase the insecticide activity of neem leaves extract solution up to 96% mortality against soybean pod sucking bug, R. linearis. The increase of the bioactivity refers to the anti insecticidal activity of ferulic acid formed from the degradation of curcumin.

Antibacterial and antibiofilm effect of silver and gold nanoparticles in Uropathogenic Escherichia coli Rini Purbowati; Sugiharto; Agusniar Furkani Listyawati; Masfufatun; Lusiani Tjandra; Noer Kumala Indahsari
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 2 (2022): June 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.2.20222

Nosocomial infections of the urinary tract are the most common nosocomial infections. Infectious diseases currently pose a life threatening due to the increasing phenomenon of infectious agent resistance. One of the factors known to cause resistance to broad-spectrum antimicrobials agent is the susceptibility to biofilm formation. The use of silver nanoparticles (NPs) is now regarded as one of the most promising strategies to target infections associated with biofilms. This study aims to determine the application of nanotechnology in the form of metal nanoparticles in targeting the formation of biofilms on infectious agents. The antibacterial and antibiofilm activity test was performed on a 96-well microtiter plate on uropathogenic Escherichia coli (UPEC) at concentrations of 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 ppm. The results showed the addition of silver and gold nanoparticles at different concentrations affected and inhibited the growth of UPEC and prevented the formation of biofilms, as indicated by a decrease in the OD value. In conclusion, silver and gold nanoparticles have antibacterial and antibiofilm effects on UPEC.

Potential improvement in the safety and quality of traditional fermented soybean products: A narrative review Budhi Setiawan; Nugroho Eko Wirawan Budianto; Masfufatun Masfufatun
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 28 No 1 (2022): December 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.28.1.20222

Soybeans are one of the most significant crops in the world due to their nutritionally valuable. It has been converted to produce a variety of fermented soybean products. During conventional soybean fermentation, microorganisms are involved, biochemical reactions occur, and bioactive components are produced. With the rise of people's living standards, customers are paying more attention not only to the flavor and nutrition of fermented soybean meals, but also to their safety and quality. Mycotoxins, biogenic amine production, and high salt content are among the public health concerns associated with fermented soybean foods. This paper reviews the prevalent concerns about the safety and quality of fermented soybean foods, as well as potential improvements. Attempts and methods have been proposed to ensure the safety of the fermentation process and food quality. Official regulations, the employment of suitable microbes, the use of high-quality cultivars, and the administration of chemicals are all viable options for improving safety and quality. We conclude that implementing international food standards, guidelines, and codes of practice such as the Codex Alimentarius for fermented soybean products and the application of scientific novel methods (e.g., starter combination, high-pressure processing, or low-dose gamma irradiation, additive usage, low salt fermentation technique) are the potential solutions to mitigate the issues and improve the safety and quality of the products.

KARAKTERISTIK BIOFILM Candida albicans DAN ANTIBIOFILMNYA Riska Ammalia Putri; masfufatun masfufatun
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 5 No 2 (2022): Medika Kartika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (475.646 KB)

Candida albicans merupakan anggota mikrobiota normal pada tubuh manusia yang sehat dengan sistem kekebalan tubuh yang baik. Namun, adanya gangguan keseimbangan akibat beberapa faktor dapat mengakibatkan C. albicans berkembang biak dengan cepat dan menyebabkan infeksi. Kemampuan C. albicans dalam menginfeksi salah satunya didukung oleh faktor virulensi, yaitu aktivitasnya dalam pembentukan biofilm. Tulisan ini bertujuan untuk mengetahui karakteristik biofilm C. albicans dan potensi zerumbone, madu jujube, dan enzim β-1,3-glukanase sebagai antibiofilm C. albicans. Hasil studi literatur berdasarkan telaah jurnal didapatkan karakteristik biofilm C. albicans memiliki perbedaan dengan organisme planktonik, hal ini seringkali dikaitkan dengan keunggulan biofilm dengan sifat resistensi dan virulensinya. Selain itu, karakteristik antar biofilm C. albicans juga dapat berbeda sesuai dengan faktor yang mempengaruhinya, perbedaan ini berhubungan dengan proses adhesi, perubahan produksi biofilm, aktivitas metabolisme, serta kekuatan perlekatan biofilm pada permukaan. Zerumbone secara signifikan diketahui menurunkan tingkat ekspresi gen terkait biofilm dan spesifik terhadap hifa, madu jujube dapat menyebabkan perubahan pada dinding sel dan eksopolisakarida, dan enzim β-1,3-glukanase memiliki kemampuan untuk mendegradasi komponen polimer matriks ekstrasel pada biofilm. Ketiga bahan antibiofilm ini diketahui dapat mencegah pembentukan biofilm dan mendegradasi biofilm C. albicans yang telah terbentuk. DOI : 10.35990/mk.v5n2.p208-219

Edukasi dan Pelayanan Kesehatan tentang Pemeriksaan Kadar Kolesterol dan Glukosa Darah Terkait Pencegahan Penyakit Jantung Koroner dan Diabetes Melitus di SMA Ta’miriyah Surabaya Noer Kumala Indahsari; Masfufatun Masfufatun; Olivia Herliani
Jurnal Abdidas Vol. 3 No. 6 (2022): December Pages 944 - 1124
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdidas.v3i6.709

Angka mortalitas Penyakit Tidak Menular (PTM), terutama Penyakit Jantung Koroner (PJK) dan Diabetes Melitus (DM), masih cukup tinggi di negara-negara berkembang. Etiologinya bersifat multifaktorial, ada yang tidak dapat dihindari namun juga ada yang dapat dihindari, oleh karena itu sejatinya dapat dilakukan langkah prevensi terhadap kejadian PTM. Kesadaran hidup sehat, seperti mengubah pola makan, aktivitas fisik dan menghentikan kebiasaan merokok, diharapkan dapat dibangkitkan melalui hasil pemeriksaan laboratorium yang nyata. Hal tersebut mendorong pengabdi untuk melakukan penyuluhan dan pelayanan kesehatan untuk meningkatkan pemahaman dan kesadaran masyarakat tentang pemeriksaan kadar glukosa darah dan kolesterol untuk menghindari PJK dan DM. Kegiatan dilakukan selama 2 hari dimana hari pertama dilakukan penyuluhan untuk memberikan edukasi dan hari kedua dilakukan pelayanan berupa pemeriksaan kesehatan dan laboratorium kepada kurang lebih 70 orang peserta dari kalangan guru dan karyawan di SMA Tamiriyah Surabaya. Hasil yang didapatkan berupa rata-rata nilai pre dan post test yang diberikan sebelum dan sesudah penyuluhan. Uji statistik Paired Sample T Test diperoleh harga sig (2-tailed) = 0,000, yaitu < 0,05 berarti ada perbedaan antara sebelum dan sesudah perlakuan. Kegiatan ini menyimpulkan bahwa penyuluhan yang diberikan dapat meningkatkan pemahaman dan kesadaran peserta tentang pentingnya pencegahan PJK dan DM.

Potensi Lidah Buaya (Aloe vera) sebagai Antimikroba dalam Menghambat Pertumbuhan Beberapa Fungi: Literature Review I Kadek Wawan Agus Wijaya; Masfufatun Masfufatun
Jurnal Kedokteran dan Kesehatan Vol 18, No 2 (2022): JURNAL KEDOKTERAN DAN KESEHATAN
Publisher : Faculty of Public Health, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jkk.18.2.202-211

Aloe vera dikatakan sebagai tanaman antimikroba karena mempunyai kandungan senyawa aktif antrakuinon yang berpotensi sebagai antibakteri dan antifungi. Banyak penelitian tentang potensi Aloe vera sebagai antifungi. Literatur review ini bertujuan untuk melengkapi informasi lebih lanjut mengenai potensi Aloe vera sebagai antimikroba dalam menghambat pertumbuhan beberapa fungi. Desain Penelitian ini adalah Literatur Review yang menggunakan penelitian sebelumnya sebagai acuan penarikan kesimpulan hasil penelitian sekarang. Sumber data berupa jurnal artikel yang diperoleh dari pencarian Science direct dan Google scholar berdasarkan topik yang telah ditentukan. Jurnal artikel yang digunakan diseleksi dari penelusuran ilmiah dengan rentang tahun 2010-2020 jurnal nasional dan internasional. Hasil dari analisis data menunjukkan bahwa kandungan kompleks Antraquinon dan Saponin, dapat berfungsi sebagai daya hambat pertumbuhan fungi. Maka dapat disimpulkan bahwa ekstrak maupun gel Aloe vera memiliki aktivitas antifungi yang cukup signifikan, namun bentuk gel Aloe vera lebih besar potensinya. Mekanisme dalam menghambat pertumbuhan fungi yaitu dengan cara menurunkan tegangan dari permukaan dinding sel dan merusak permeabilitas membran sehingga terjadi kebocoran protein dari dalam sel.

Analisis Beberapa Jenis Vaksin Covid-19 yang akan Dipakai di Indonesia MASFUFATUN; NI PUTU MANIK SURYANINGSIH; I MADE DWIKY KARINA JAYA; ALMAIDAH SAFITRI; TEOFILUS DANI PRASETYOADI; JAMES HADIPUTRA SUNARPO
HANG TUAH MEDICAL JOURNAL Vol 20 No 1 (2022): Hang Tuah Medical Journal
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/htmj.v20i1.325

At the end of 2019, there was a pandemic that originated in Wuhan, China. This pandemic is known as the corona virus / COVID-19. COVID-19 itself is caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2). In Indonesia alone, COVID-19 cases have reached 1.17 million and those who died have reached 31,976 people. From this case, experts are racing to find this COVID-19 vaccine. The COVID-19 vaccine itself is expected to control the spread of the virus in the world. This review article aims to discuss the various types of vaccines that will be used in Indonesia in terms of types, methods of action, efficacy, specificity, sensitivity, dosage, and side effects. The results of the review article show that vaccines are one of the most effective and economical methods. Various studies have been carried out by several experts from around the world to develop a vaccine for COVID-19. Each vaccine used has the same goal, namely to control the spread of the COVID-19 virus which is currently still a pandemic around the world. Based on the results of the review article, it can be concluded that all types of vaccines have their respective advantages and disadvantages. Thus, the community will not feel worried and are more confident in determining what kind of vaccine will be taken.

Characteristics of Indonesian Wild honey and Cultivited Honey and Their Antibacterial Activity against Staphylococcus aureus and Escherichia coli Lusiani Tjandra; Budhi Setyawan; Masfufatun masfufatun
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 11, No 1 (2022): MARET 2022
Publisher : Universitas Wijaya Kusuma Surabaya

Indonesia's natural wealth is very abundant in the form of flora and fauna that can be developed as raw materials for medicine. Honey in Indonesia is very diverse from Sabang to Merauke. Different types of honey are influenced by regional origin, season at harvest, type of bee, type of plant source of nectar, way of life of bees (cultivated or wild), harvesting method and post-harvest handling. This study aims to determine the characteristics of forest bee honey and cultivated honey and to determine the potential of honey as an antibacterial in the treatment of infectious diseases caused by Staphylococcus aureus and Escherichia Coli bacteria. The materials used were Carisa honey samples from Wild honey [Wild Klanceng and Wild Cerana] and Cultivation [Cerana Cultivation], S. aureus and E. coli bacteria. The characteristic test method is in accordance with the Indonesian National Standard and the honey inhibition test against the growth of S. aureus and E. Coli bacteria using the diffusion method. The results showed that Wild Cerana (WC) and Wild Klanceng (WK) honey demonstrated higher water content, ash content, acidity and glucose from Cerana Cultivated (CC) honey. Carissa Honey (beside Cerana cultivated honey) had antibacterial activity against S. aureus and E. Coli at different concentrations. Wild Wild Honey has the highest antibacterial activity compared to other types of honey. Conclusion Indonesian wild honey showed weak antibacterial activity against Staphylococcus aureus. Meanwhile, honey that is cultivated does not have antibacterial activity.

INCIDENCE OF ORAL CANDIDIASIS IN FIXED ORTHODONTIC APPLIANCES Wahyuni Dyah Parmasari; Masfufatun; Tri Juliati
Interdental Jurnal Kedokteran Gigi (IJKG) Vol. 20 No. 1 (2024): Interdental Jurnal Kedokteran Gigi (IJKG)
Publisher : Fakultas Kedokteran Gigi, Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46862/interdental.v20i1.8505

Introductions: Oral candidiasis is most often caused by Candida albicans, followed by C. glabrata. In European countries, an analysis showed that more than half of candidiasis cases were caused by C. albicans and the infection rate caused by other candida strains was 14% for C. glabrata and C. parapsilosis, 7% for C. tropicalis and 2% for C. krusei. According to a study in Brazil, C. albicans accounted for 40.9% of cases followed by C. tropicalis (20.9%), C. parapsilosis (20.5%) and C. glabrata (4.9%). Meanwhile, in Indonesia, there is no national data regarding the epidemiology of oral candidiasis in Indonesia. Hospital Dr. Hasan Sadikin (RSHS) Bandung conducted research from 2010-2014 and found that during that period 49 patients had oral candidiasis. In general, side effects can be felt by patients who are in fixed orthodontic treatment which affects comfort and quality of life, namely 42.79% experience a sensation of pain in the area of the oral cavity, while 51.80% complain of discomfort when chewing food and this can indicate that Orthodontic appliances are mainly the patient's daily activities, the second is related to chewing food, phonetics and smile lines. The purpose of this study was to determine the incidence of candidiasis on orthodontic appliances. This study was to determine the incidence of candidiasis on fix orthodontic appliances. Material and Methods: In this study, 80 samples were obtained from the total population, namely medical students who use fixed orthodontics with metal materials. Methods with SWAB examination on the surface of the bracket and the oral mucosa that intersect with the ortho-adhesive device. The results were recorded and then data analysis was carried out. Then the specimens were put in a microbiology incubator for 48 hours at room temperature. If candida albicans grows on the petridish then it is assessed that candidiasis is present. Results: From the study, it was found that 80% incidence of candidiasis, 20% is normal. Conclussions: There is a high incidence of oral candidiasis in patients using fix orthodontics appliances.

Co-Authors Adi Candra, Adi AFAF BAKTIR akhmad sudibya, akhmad ALMAIDAH SAFITRI Amalia Nadiasari Amrulloh, Azmi Khalid Ananta Sandi Putra Ananta, Zahrah Aswa Andini Risma Ayunda Dewi Andriani, Chenny Aritania, Putu Oky Atik Sri Wulandari Ayly Soekanto Ayu Cahyani Noviana Budhi Setiawan Budhi Setiawan Budhi Setyawan Chenny A Wiyono Chenny Andriani Wiyono Dadik Raharjo Emilia Devi D.R, Emilia Devi Fardiansyah, Al Farizah El Husna Fehima Syachsiyat Alya Firdausi Nuzula Habibi, Miftahul Handy Arief HARMAN AGUSAPUTRA Harsono Harsono Haryanto, Loo Herliani, Olivia I Kadek Wawan Agus Wijaya I MADE DWIKY KARINA JAYA Inawati Inawati Inawati Inawati Indahasari, Noer Kumala Islam, Afif Fajrul Isnaeni, Mutia Jalal, Ismul JAMES HADIPUTRA SUNARPO Joeniarti, Elika Jonathan Kartika Ishartadiati Kuntaman Kuntaman Laili Shafarina Fauzan Laily, Aulia Nur Listyawati, Agusniar Furkani Loo Hariyanto Raharjo, Loo Hariyanto Lusiani Tjandra Meilya Putri Syahida Mentari, Andi Oktaviana Mohammad Ali Izzudin Muhammad Rozzan Prananda Narotama, Harya Ni Luh Narita Vijayanti Ni Made Putri Pradnyandari NI PUTU MANIK SURYANINGSIH Noer Kumala Noer Kumala NOER KUMALA Noer Kumala Indahsari, Noer Kumala NUGROHO EKO WIRAWAN BUDIANTO Nur Kumala Indahsari, Nur Kumala Nur Kumala, Nur Nurma Fitri Parawidnyaningsih, Putu Ayu Aryanda Pramita Laksitarahmi Isrianto Pratika Yuhyi Hernanda Putu Oky Ari Tania Qurraty’ain, Seryna Hasna Rachmawati Dwi Apriliyani Ratnasari, Diana Tri Rini Purbowati Rini Purbowati Riska Ammalia Putri Risnanda, Kadek Rio Setijowati, Eva Diah Soebandhi, Santirianingrum Sri Lestari Utami Sugiharto Suhartati Suhartati Suhartati Syahrefi Syahrefi TEOFILUS DANI PRASETYOADI Tikapa Muwawiyai Pigome Tri Juliati Tri Rahayuningsih Tri Ratnasari, Diana Utami, Sri Lestari Vini Anggraini Wahyuni Dyah Parmasari, Wahyuni Dyah Wibisono, Freegied Satriya Wibisono, Freshinta Jellia Yoga Yuniadi

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