Articles
Red fruit oil increases trophoblast cells and decreases caspase-9 expression in placenta of lead exposed mice
Sumarsono, Portia;
Widjiati, Widjiati;
Susilowati, Suherni
Universa Medicina Vol 35, No 2 (2016)
Publisher : Faculty of Medicine, Trisakti University
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DOI: 10.18051/UnivMed.2016.v35.110-118
BACKGROUNDLead is able to pass through the placental barrier and interfere with fetal development. Red fruit has high antioxidant activity, due to carotenoids (pro-vitamin A), tocopherols (vitamin E) and unsaturated fatty acids. This study aims to examine the effect of red fruit oil towards placenta in pregnant mice before lead exposure.METHODSThis was a laboratory experimental post test only study, using 20 pregnant mice (Mus musculus) that were randomized into five groups. The negative control group was not exposed to lead; intervention group 0 (P0) was exposed to lead at 0.011 mg/20 g BW/day on days 6-15 of gestation. The intervention groups P1-P3 were given red fruit orally at 0.3 mL/20 g BW/day, 0.8 mL/20 g BW/day, 0.9 mL/20 g BW/day, respectively, before lead exposure. Normal trophoblast cell count and caspase-9 expression of trophoblasts were calculated. One-way ANOVA, Kruskal-Wallis and Mann Whitney tests were used to analyze the data.RESULTSThe intervention groups P2 and P3 given red fruit at 0.8 mL/20 g BW/day and 0.9 mL/20 g BW/day showed a significant increase in normal trophoblast cell count of 88.2% (p=0.024) and a decrease in caspase-9 expression of 70.8% compared with P0 to which was not given red fruit (p=0.020). CONCLUSIONRed fruit increased normal trophoblast cell count by 88.2% and decreased caspase-9 expression by 70.8% in pregnant mice before lead exposure. It should be noted that red fuit may prevent oxidative damage to cells.
Effects of Curcumin on Vascular Endothelial Growth Factor Expression on Rattus norvegicus Cervical Cancer Xenograft Model
Muninggar, Laili;
Widjiati, Widjiati;
Yuliati, Indra;
Askandar, Brahmana;
Hartono, Poedjo
Indonesian Journal of Cancer Vol 12, No 3 (2018): July-September
Publisher : National Cancer Center - Dharmais Cancer Hospital
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DOI: 10.33371/ijoc.v12i3.616
Objective: To analyze the effect of curcumin in VEGF expression on Rattus norvegicus cervical cancer cell xenograft model.Methods: An experimental study with randomized post test only control group design. The subjects were Rattus norvegicus (Sprague Dawley), inoculated with He-la cervical cancer cells from American Type Culture Collection (ATCC) processed in stem cell laboratory Institute of Tropical Disease (ITD) Airlangga University. 5x106 of He-La cells were injected subcutaneously in dorsal flank area of Rattus norvegicus. After 30 days of observation we performed histopathological examination of xenograft tissue and randomized into 2 groups which were given curcumin orally 1000 mg/kg (curcumin group) vs. no therapy (control group). After another 30 days the xenograft tissue was dissected and underwent immunochemistry examination for VEGF expression.Results: 32 samples of Rattus norvegicus were divided into 2 groups, In curcumin group the VEGF median expression was 2,2 (0,3-7,6) and in control group the VEGF median expression was 6,6 (1,2-12). There was a statistically significant difference with p value =0,009 with Mann Whitney test (p<0,05).Conclusion: VEGF expression in Rattus norvegicus xenograft model of cervical cancer was suppressed by giving Curcumin 1000 mg/kgBB orally.
MOZART MUSIC INCREASES THE NUMBER OF GLIAL CELLS COMPARED TO INDONESIA POP AND RELIGIOUS MUSIC
Rahayu, Pipit Sri Estuning;
Hermanto, Hermanto;
Widjiati, Widjiati
Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga Vol 15, No 2 (2019): December 2019
Publisher : Universitas Muhammadiyah Malang
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DOI: 10.22219/sm.Vol15.SMUMM2.10265
Music stimulation is an important component for prenatal fetal development. Both pop and religious music are easy to listen and widely accepted in Indonesia. This study was to analyze the effect of Mozart, pop, and religious music exposure during pregnancy to the number of glial cells in the brain of Rattus norvegicus offspring. The samples were divided into three groups based on the exposure for each group, namely Mozart, pop, and religious music, duration of 60 minutes with 65dB intensity, initiated on the 10th day of pregnancy for 9 days in the soundproof chamber. Three brains of the offsprings were dissected and prepared for Hematoxylin-Eosin staining counted on 5 fields of view and 400 magnification strength.Different glial cells number of Rattus norvegicus brain between groups were observed. Mozart music (28,29) showed a highest mean and pop music (18,67) showed the lowest mean. Significant difference of the number of brain glial cells between Mozart music compared to pop and religious music groups were observed, with p value <0,005.The number of brain glial cells of Rattus norvegicus offsprings in the Mozart group were significantly higher than those in pop and religious groups.
Synapsis Expression Increased By Mozart’s Music Stimulation Compared With Indonesian Pop And Religious Music
Amila, Ukhti Mukminah ilmi;
Joewono, Hermanto Tri;
Widjiati, Widjiati
Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga Vol 16, No 1 (2020): June 2020
Publisher : Universitas Muhammadiyah Malang
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DOI: 10.22219/sm.Vol16.SMUMM1.10784
Classical music stimulation is a proven method that affect intelligence as well as brain function. Mozart’s classical music is classical music that could stimulate the growth of synaptic networks. To analyze the difference of synapsin expression of the brain of Rattus norvegicus offspring on Mozart’s, pop, religious and without music stimulation during pregnancy. Experimental. Treatments were subjected to Rattus norvegicusas a subject organism, initiated on the 10th day of pregnancy, in 1 hour duration in a soundproof chamber, intensity of 65 dB. On the 19th day of pregnancy the subjects were sacrificed and three offsprings were chosen. The head of the chosen Rattus norvegicusoffsprings were decapitated and the brain subsequently sectioned to calculate synapsin expression by immunohistochemistry method with 400 magnification strength microscope on the 5 field of view. Significant difference of synapsin expression of the brain of Rattus norvegicus offspring was observed between Mozart’s and control group. Significant differences of synapsin expressions were observed in the cerebellum of the newly born Rattus novergicus between Mozart and pop music groups (p = 0,007), Mozart and religious music groups (p = 0,008), Mozart and without music groups (p = 0,004).Synapsin expression of Rattus norvegicus brain offsprings were higher in Mozart music stimulation compared with pop and religious music stimulation
Combined Effect of Physical and Psychological Stress Exposure during Pregnancy on the Expression of Caspase-3 Cerebrum and Cerebellum of Newborn Mus musculus
Binta Dwi Novitasari;
Hermanto Tri Joewono;
Widjiati
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v15i4.16751
Background: Prenatal stress affects fetal development including brain development. When a stressor isfelt, the brain as the main target for stress will release a hormone that stimulates the release of pro-apoptoticproteins and activate caspase-3 which acts as an executioner caspase in the cell death process. The aim of thestudy was to analyze the effect of combined stress during pregnancy on the expression of caspase-3 cerebrumand cerebellum of newborn Mus musculus. Methods: An experimental study using 24 pregnant mice (Musmusculus). Subjects were randomized into four groups, consisting of physical stress exposure group (forcedswimming) (G1), psychological stress exposure group (noise) (G2), combination stress exposure group(forced swimming + noise) (G3), and control group (G4). Stress exposure was given on 6th-15th days ofpregnancy. From each mother, three Newborn of Mus musculus were taken to make preparations from braintissue. Immunohistochemical examination was performed to assess caspase-3 expression. Results: Thestudy shows that the mean and standard deviation of the expression of caspase-3 cerebrum and cerebellumin the physical stress exposure group is 5.70 ± 0.99 and 5.80 ± 1.35, the psychological stress exposure groupis 7.23 ± 1.39 and 7.40 ± 1.24, the combined stress exposure group is 8.67 ± 1.09 and 9.30 ± 1.12, and thecontrol group 4.17 ± 1.18 and 3.90 ± 1.06. ANOVAs statistical test results show significant differencesamong groups with a value of p = 0.000 in the cerebrum and p = 0.000 in the cerebellum. Conclusion:Exposure to physical and psychological stress during pregnancy increases the expression of caspase-3 in thecerebrum and cerebellum of newborn mice.
Effect of Murotal Sound Stimulation during Pregnancy on the Number of Neuron Cells of Cerebrum and Cerebellum of the Newborn Rattus norvegicus
Ayu Putri Yani;
Hermanto Tri Joewono;
Widjiati
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v15i4.16809
Background: The human development index (HDI) illustrates how superior human resources are. Thestimulation of Murotal during growth and development has been shown to increase cell growth in plants.The aim of the study was to analyze the effect of the differences againts the number of neuron cell’s in thecerebrum and cerebellum Rattus norvegicus offspring between those who received Al-quran Murottal surahAr-Rahman stimulation, Qori and Qoriah sounds voice in pregnancy day of 6th. Methods: An Experimentalstudy using 30 pregnant (Rattus norvegicus). Subjects were randomized into three groups, control group(Q1), voice of Qori group (Q2), and voice of Qoriah group (Q3). sound stimulation was given on 6th-17thdays of pregnancy. From each mother, two Newborn of Rattus norvegicus were taken to make preparationsfrom brain tissue. Eosin Hematocycline was performed to assess number of neuron cells. Results : Thestudy shows that the mean and standard deviation of the number neuron cells cerebrum and cerebellum inthe control group is 9.88 ± 1.71 and 21.10 ± 2.11, the voice qori group is 10.16 ± 1.01 and 22.46 ± 1.71,and the voice qoriah group 11.12 ± 1.52 and 22.50 ± 1.76. ANOVAs statistical test results show there isno significant differences among groups with a value of p = 0.152 in the cerebrum and p=0.183 in thecerebellum. Conclusion : Murotal sound stimulation during pregnancy increased the number of neuron cellsin the cerebrum and cerebellum of newborn rats.
Combined Effect of Physical and Psychological Stress Exposure during Pregnancy on the Expression of Caspase-3 Cerebrum and Cerebellum of Newborn Mus Musculus
Binta Dwi Novitasari;
Hermanto Tri Joewono;
Widjiati
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v15i4.16817
Background: Prenatal stress affects fetal development including brain development. When a stressor isfelt, the brain as the main target for stress will release a hormone that stimulates the release of pro-apoptoticproteins and activate caspase-3 which acts as an executioner caspase in the cell death process. The aim of thestudy was to analyze the effect of combined stress during pregnancy on the expression of caspase-3 cerebrumand cerebellum of newborn Mus musculus. Methods: An experimental study using 24 pregnant mice (Musmusculus). Subjects were randomized into four groups, consisting of physical stress exposure group (forcedswimming) (G1), psychological stress exposure group (noise) (G2), combination stress exposure group(forced swimming + noise) (G3), and control group (G4). Stress exposure was given on 6th-15th days ofpregnancy. From each mother, three Newborn of Mus musculus were taken to make preparations from braintissue. Immunohistochemical examination was performed to assess caspase-3 expression. Results: Thestudy shows that the mean and standard deviation of the expression of caspase-3 cerebrum and cerebellumin the physical stress exposure group is 5.70 ± 0.99 and 5.80 ± 1.35, the psychological stress exposure groupis 7.23 ± 1.39 and 7.40 ± 1.24, the combined stress exposure group is 8.67 ± 1.09 and 9.30 ± 1.12, and thecontrol group 4.17 ± 1.18 and 3.90 ± 1.06. ANOVAs statistical test results show significant differencesamong groups with a value of p = 0.000 in the cerebrum and p = 0.000 in the cerebellum. Conclusion:Exposure to physical and psychological stress during pregnancy increases the expression of caspase-3 in thecerebrum and cerebellum of newborn mice.
The Effect of Exposure Qari and Qariah Recitation During Pregnancy to the Number of Astrocyte Glia Cells in the Cerebrum Newborn Rattus Norvegicus
Dwi Nurdi Puspita Sari;
Widjiati;
Hermanto Tri Joewono
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v15i4.16840
The quality of human resources should be prepared since in early stage. Pregnancy is one of a significantperiod which can take advantage to by providing proper nutrition and adequate stimulation. For instance,sound and music, the most harmonious combination and easily accepted by the fetus(1). Experimentalanalytic type with post-test only control group design was employed on 30 pregnant Rattus norvegicus whichwere divided randomly into 3 groups, namely the control group, qari group, and qariah group. Each of thegroups had stimulation starting 6th to 17th days of pregnancy for 60 minutes. At 18 days of pregnancy, themother of Rattus Norvegicus was sacrificed and bring forth with Sectio Caesarea (SC). As a result, thenumber of astrocyte glia cells in the cerebrum was higher in the qaria recitation stimulation group (22.62 ±3.75), compared with the group that was listened to qari recitation stimulation group (19.84 ± 2.48), whilethe control group (16.54 ± 2.78). A significant difference found in the number of astrocyte glia cells inthe Cerebrum Rattus norvegiccus newborn that were listened to the qari and qariah recitation stimulationgroup and the control group. The highest number of Glia cells is obtained from the stimulation of qariahrecitation.
Qari (Male Voice) Increases Higher Expression of Synapsin 1 than Qariah (Female Voice) in the Cerebrum Newborn Rattus Norvegicus
Nur Laila Faizah;
Hermanto Tri Joewono;
Widjiati
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v15i4.17002
Introduction: Pregnancy is the golden period to prepare children for the future. Stimulation is neededduring pregnancy, one of which is brain stimulation. This can be done with Al Qur’an Murrotal SurahAr-Rahman stimulation. This study aims to analyze expression of synapsin 1 in the cerebrum of newbornRattus norvegicus when given murrotal Surah Ar-Rahman stimulation by qari, qariah, and not given anystimulation during pregnancy.Methods: Experimental with post-test only control group design. Sampling in this study was consideredhomogeneous because it met the inclusion criteria. The samples obtained were distributed randomly to eachexperimental group and randomized into 3 groups, including control group, group stimulated by qari, andgroup stimulated by qariah. The treatment was given on the 6th day of pregnancy for 1 hour in a soundproofroom with an intensity of 65 dB.Results: The highest mean expression of synapsin 1 in the cerebrum is qari group (6.96 ± 2.59), then qariahgroup (5.84 ± 2.90), and the lowest is control group (5.74 ± 2.32). There is no significant difference inexpression of synapsin 1 in the cerebrum between control and qari groups (p = 0.306), control and qariahgroups (p = 0.932), qari and qariah groups (p = 0.347).Conclusion: The highest expression of Synapsin 1 in the cerebrum of newborn Rattus norvegicus is thegroup stimulated by qari.
Stimulation of Male Voice during Pregnancy Results in Higher Expression of Brain Derived Neurotrophic Factor in Cerebellum of Newborn Rattus norvegicus
Nurvy Alief Aidillah;
Hermanto Tri Joewono;
Widjiati
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd
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DOI: 10.37506/ijfmt.v15i4.17011
Background: Recent studies in the field of Fertomaternal Medicine show that stimulation in the uteruscan support brain growth and development. If in previous studies stimulation using several types of music,this study uses human voice which aims to analyze the effect of stimulation of male and female voices ondifferences in the expression of Brain Derived Neutrotrophic Factor (BDNF) in cerebellum of newbornRattus norvegicus. Methods: This type of research is true experimental with post test only control groupdesign. Rattus norvegicus was given stimulation of men voice and women voice on 6th day until 17th dayof pregnancy. The expression of BDNF was examined using immunohistochemistry. Results: The mean ±standard deviation of the expression of BDNF in the cerebellum is 4.20 ± 1.75 (without stimulation), 6.04± 1.58 (male voice stimulation), and 5.60 ± 2.43 (female voice stimulation). The results of statistical testsshow that there is a significant difference on BDNF expression between the control group and the groupstimulated with male voice with a significance value <0.05 of 0.045 Conclusion: Giving stimulation usingthe male voice during pregnancy shows a significant increase in the expression of BDNF in cerebellum ofnewborn Rattus norvegicus.