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All Journal Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Indonesian Journal of Cancer The Indonesian Biomedical Journal Universa Medicina Journal Of Vocational Health Studies Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Jurnal Medik Veteriner Care : Jurnal Ilmiah Ilmu Kesehatan Ovozoa: Journal of Animal Reproduction Bioscientist : Jurnal Ilmiah Biologi journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Current Biomedicine Journal of Education Technology and Inovation Folia Medica Indonesiana

Widjiati Widjiati, Widjiati

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Author-ID : 596972

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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EFEK PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL LEYDIG MENCIT (Mus musculus) JANTAN YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN Nursadida, Milla; Plumeriastuti, Hani; Dhamayanti, Yeni; Widjiati, Widjiati; Luqman, Epy Muhammad; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19998

This study was aim to examine the effect of kebar grass extract (Biophytum petersianum Klotzsch) to against number leydig cells of mice (Mus musculus) by exposed 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD). Twenty five male mice (Mus musculus) 4 months with avarage body weight 20 g were used. These animals were divided into five groups (K(-), K(+), P1, P2 and P3). K(-) was treated with placebo, K(+) was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw, P1 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,045 mg/g Bw/day P2 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,080 mg/g Bw/day, P3 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 1,350 mg/g Bw/day. This research has been conducted for 53 days. The data were compared using ANOVA and Duncan test by SPSS 22.4 for windows. The result showed that Kebar Grass Extract in all of groups can prevent the damage of leydig cells in testis that exposed by TCCD significantly (p<0,05) and kebar grass extract 0,135 mg/kgBw/day can increase amount of leydig cells maximaly.

PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP GAMBARAN HISTOPATOLOGI BRONKUS DAN VENA PULMONALIS MENCIT (Mus musculus) JANTAN YANG DIPAPAR ASAP ROKOK Jeanita, Winny; Meles, Dewa Ketut; WIdjiati, Widjiati; Hamid, Iwan Sahrial; Luqman, Epy Muhammad; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21089

This research aimed to attest the effect of Kepok banana (Musa acuminata) peel extract on the histopathology of bronchus and pulmonary veins in male mice (Mus musculus) exposed by cigarette smoke. The 24 experimental male mice were divided into six groups with each treatment having four replicate. All the groups, except K- as a control group, was treated with cigarette smoke for 14 days. After that, K- and K+ was treated with CMC-Na 1%, KO was treated with ascorbic acid 13 mg/kgBW, P1 was treated with Kepok banana peel extract 14 mg/kgBW, P2 was treated with Kepok banana peel extract 28 mg/kgBW, and P3 was treated with Kepok banana peel extract 56 mg/kgBW for 14 days. The data of the thickness of bronchial epithelium and the diameter of pulmonary veins were analyzed using ANOVA (Analysis of Varience) with significance p<0,05 and continued with Post- hoc Tukey. The result of the thickness of bronchial epithelium showed that K+ had the highest thickening (431,83 + 9,71 µm) and significant differences (P<0,05) with all the groups. The result of P3 showed the lowest thickening of bronchial epithelium (170,84 + 2,70 µm). The result of pulmonary veins diameter showed that K+ had the narrowest diameter (525,64 + 16,61 µm) and significant differences (p<0,05) with all the groups. The result of P3 showed the lowest diameter narrowing of pulmonary veins (718,10 + 9,64 µm). The conclussion of this research are Kepok banana peel extract able to decreased the thickness of bronchial epithelium and the narrowing of pulmonary veins diameter in male mice with effective dose 28 mg/ kgBW.

STEM CELL THERAPY ON ANIMAL MODEL (Rattus norvegicus) END GESTATIONAL TIME EXPOSED TO CARBON BLACK AS OBSERVED FROM CASPASE-3 EXPRESSION Achmad, Agung Budianto; Madyawati, Sri Pantja; Widjiati, Widjiati
Journal of Vocational Health Studies Vol. 1 No. 3 (2018): March 2018 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V1.I3.2018.102-106

Background: Air pollution in the form of Diesel Exhaust Particles emerging from motor vehicles are harmful to health that have adverse reproductive health impacts, especially during pregnancy. The use of stem cells in treating white mice (Rattus norvegicus) exposed to carbon black can reveal the potential for treatment of placental impairment during pregnancy. Purpose: to demonstrate the effectivity of Rat Bone Marrow Mesenchymal Stem Cell therapy on rats (Rattus norvegicus) exposed to carbon black as observed from caspase-3 expression. Method: This research uses a completely randomized design with factorial pattern. Forty-eight gravid female rats were divided into six treatment groups. Result: caspase-3 expression in each treatment showed no significant differences in the groups treated with RBMMSC in each gravid groups treatment (therapy GD 11 and GD 17) were exposed to carbon black and not treated with RBMMSC. The same are indicated by the normal trophoblast cells (cytotrophoblast and syncytiotrophoblast cells) in the RBMMSC treated group showed no significant difference with the group exposed to carbon black only. Conclusion: this research indicate that Rat Bone Marrow Mesenchymal Stem Cell therapy in Rattus norvegicus exposed to carbon black have not been able to reduce expression of caspase-3.

Impact of Freeze-Dried Amnion Membrane and Human Amnion Stem Cell Seeding on TGF-B and Collagen Type III in Vesicovaginal Fistula Sihotang, Jojor; Mardiyan, Eighty; Widjiati, Widjiati; Hidayatullah, Amiruddin
Indonesian Journal of Obstetrics and Gynecology Volume 12 No. 4 October 2024
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32771/inajog.v12i4.2139

Objective: to analize how freeze-dried amniotic membrane and human amniotic stem cell seeding affect TGF B and type III collagen expression in suturing a New Zealand rabbit vesicovaginal fistula model. Metode: This experimental study employed New Zealand rabbits and a vesicovaginal fistula model with a post-test only control group design. The rabbits were divided into 3 groups: vesicovaginal fistula suturing alone, suturing with freeze-dried amniotic membrane, and suturing with freeze-dried amnion-seeded stem cells. After 7 days of treatment, specimens near the repaired vesicovaginal fistula were collected for immunohistochemical analysis of TGF B and collagen type III expression. Result: TGF B expression was significantly higher in the freeze-dried amniotic membrane with stem cell seeding group (p=0.001) compared to the freeze-dried amniotic membrane without stem cell seeding group (p=0.017) and the suturing-only group (p=0.049). Additionally, type III collagen expression was significantly elevated in the freeze-dried amnion membrane and stem cell seeding group (p=0.001) compared to the freeze-dried amnion group without stem cell seeding (p=0.09) and the suturing-only group (p=0.026). Conclusion: The expression of TGF B and type III collagen was higher in rabbits with vesicovaginal fistulas treated using freeze-dried amnion and amniotic stem cell seeding compared to those without amniotic stem cell seeding and vesicovaginal fistula suturing alone. Keywords: Vesicovaginal Fistula, Freeze-dried Amnion, Stem cell

Pengaruh Pemberian Nanocurcumin (Curcuma longa) Terhadap Gambaran Histopatologis Pulmo Tikus (Rattus norvegicus) Yang Dipapar Carbon Black Rahmaniabdi, El Roy Achmad; Mustofa, Imam; Hestianah, Eka Pramyrtha; Widjiati, Widjiati; Kurnijasanti, Rochmah; Plumeriastuti, Hani
Current Biomedicine Vol. 3 No. 1 (2025): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.1.38

Background Exposure to carbon black can induce respiratory toxicity, leading to tissue remodeling, inflammation, and fibrosis. Curcumin, a compound from Curcuma longa, has been shown to exert protective effects on lung tissue by inhibiting cell proliferation and reducing alveolar epithelial thickening and inflammatory responses. Objective This study aimed to evaluate the effect of nanocurcumin administration on alveolar septal thickness and fibrosis in the lungs of rats (Rattus norvegicus) exposed to carbon black. Methods Thirty female rats were divided into five groups: the negative control group (K-) received distilled water without carbon black exposure; the positive control group (K+) received distilled water and was exposed to carbon black at a dose of 1064 mg/m³ for 6 hours; and treatment groups P1, P2, and P3 received nanocurcumin at doses of 50, 100, and 200 mg/kg body weight, respectively, along with carbon black exposure at a dose of 1064 mg/m³ for 6 hours/day over 30 days. Results Alveolar septal thickness and fibrosis in the P1, P2, and P3 groups showed significant reductions (P<0.05) compared to the K+ group. Notably, groups P2 and P3 exhibited similar alveolar septal thickness to the K- group, with statistically lower (P<0.05) fibrosis levels than P1 but not significantly different from K- (P>0.05). Conclusion Nanocurcumin administration demonstrates potential in mitigating alveolar septal thickening and fibrosis in rat lungs subjected to carbon black exposure, suggesting a protective role against pulmonary damage from particulate matter.

Role of Cryoprotectants in Preventing Quality Loss of Goat Oocytes after Warming by Examining Insulin-Like Growth Factor-1 and Bone Morphogenetic Protein-15 Expression Shabira, Zahra; Widjiati, Widjiati; Rimayanti, Rimayanti; Lestari, Tita Damayanti; Safitri, Erma; Mafruchati, Maslichah; Hendrawan, Viski Fitri
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.175-182

This research aimed to assess the efficacy of ethylene glycol-sucrose as an alternative cryoprotectant compared to commercial cryoprotectants by focusing on the expression of insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-15 (BMP-15) in goat oocyte cumulus complexes after warming with identification using the immunocytochemical analysis. Maturated oocytes were divided into 3 groups, i.e., (C) the control group followed by an immunocytochemical examination, and two treatment groups, i.e., (P1) was exposed to a commercial cryoprotectant for 15 minutes, then placed in a hemistraw and dipped in liquid nitrogen, and (P2) was exposed to 30% ethylene glycol and 1 M sucrose for 15 minutes, then placed in a hemistraw and dipped in liquid nitrogen. The results revealed divergent responses in IGF-1 (CG = 9.00 ± 3.00; P1 = 9.50 ± 3.20; P2 = 4.67 ± 0.94) and BMP-15 (CG = 10.50 ± 3.35; P1 = 9.50 ± 2.69; P2 = 5.50 ± 3.64) expression patterns, possibly influenced by the two cryoprotectant abilities and oocytes performing their permeabilities to cryoprotectant solution. We concluded that each cryoprotectant is necessary to mitigate the adverse impact on gene expression in oocytes so that we can use it for reproductive technologies.

Quercetin Stabilizes Atherosclerotic Plaques by Reducing Matrix Metalloproteinase-9 Expression and Enhancing M2 Macrophage Activity in Wistar Rats Ermawan, Romi; Pikir, Budi Susetyo; Mulyanto, Mulyanto; Utomo, Budi; Widjiati, Widjiati; Oktaviono, Yudi Her
The Indonesian Biomedical Journal Vol 17, No 5 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i5.3790

BACKGROUND: Quercetin has been shown to alleviate and prevent atherosclerosis. However, its role in stabilizing atherosclerotic plaques to prevent plaque rupture remains unclear. Therefore, this study was conducted to investigate the effects of quercetin on stabilizing atherosclerotic plaques.METHODS: Thirty-two Wistar rats were objected to a high-fat diet, along with an endothelial injury procedure conducted during the second week to create atherosclerotic plaque models. After six weeks, the subjects were randomly assigned to five groups consist of two control groups and three treatment groups treated with different quercetin dosages. Following the treatment, all subjects were euthanized to collect the left common carotid artery. The stability of the atherosclerotic plaques was evaluated by measuring the expression of matrix metalloproteinase-9 (MMP-9) using real-time polymerase chain reaction, assessing the activity of M1 and M2 macrophages along with the M1/M2 ratio using an enzyme-linked immunosorbent assay, and determining the maximum intima thickness through histopathological examination.RESULTS: Quercetin significantly reduced the expression of MMP-9, increased the activity of M2 macrophages, and lowered the M1/M2 ratio at doses of 10 and 50 mg/kg. However, there was no effect on M1 macrophage activity or maximum intima thickness. Path analysis indicated that quercetin primarily enhanced atherosclerotic plaque stability by reducing MMP-9 expression (p<0.001) and subsequently enhancing M2 macrophage activity (p=0.002).CONCLUSION: Quercetin administration significantly decreased the expression of MMP-9, enhanced the activity of M2 macrophages, and lowered the M1/M2 ratio at specific doses. These findings emphasize the significance of quercetin in stabilizing atherosclerotic plaques.KEYWORDS: atherosclerotic plaque, quercetin, stability, Wistar rats

PENGARUH PEMBERIAN INSULIN-LIKE GROWTH FACTOR-I (IGF-I) DARI SERUM KUDA CROSSBREED BUNTING TERHADAP KETEBALAN ENDOMETRIUM MENCIT (Mus musculus) Risna Y, Dyah Ayu Roro; Mulyati, Sri; Darsono, Roesno; Mustofa, Imam; Arimbi, Arimbi; Widjiati, Widjiati
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of the research was to know the effect of Insulin-Like Growth Factor-I (IGFI) derived from pregnant crossbreed mare serum (PMS) on endometrium thickness of mice (Mus musculus). The subject of this research were 35 female mice. The research was arranged by Completely Randomized Design (CRD) with seven treatment and five replications. The treatment were P0 = 10 ng/ml of physiological NaCl, P11 = 10 ng/ml of IGF-I PMS, P12 = 20 ng/ml of IGF-I PMS, P13 = 40 ng/ml of IGF-I PMS, P21 = 10 ng/ml of IGF-I recombinant mouse, P22 = 20 ng/ml of IGF-I recombinant mouse, and P23 = 40 ng/ml of IGF-I recombinant mouse. Observed variables include histopatological endometrium thickness of mice. The data were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The data was also be analyzed using General Linear Model Univarieted to see the comparison between IGF-I PMS and recombinant mouse. The result showed that the addition of IGF-I PMS did not significantly affect (p>0,05) on endometrium thickness of mice . It showed that did not significantly difference (p>0,05) between the effect of IGF-I PMS and IGF-I recombinant mouse against the endometrium thickness of mice.

Pengaruh urea dalam media maturasi in vitro terhadap tingkat maturasi oosit sapi Kurniawati, Sepvian Dewi; Sarudji, Suryanie; Widjiati, Widjiati
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 2 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i2.2021.46-52

This study was aimed to determine the effect of urea in maturation medium on in vitro oocyte maturation rate. The medium used was TCM-199 added with Hepes, NaHCO3, Kanamycin 0.15 IU/mL, PMSG, 0.15 IU/mL hCG, and 10% FBS. Cumulus oocyte complexes (COCs) of cows derived from follicle aspiration were divided into three groups. In control group (P0), the COCs were matured in vitro in a maturation medium without urea addition, meanwhile in the P1 and P2 groups, the medium was added with urea 20 and 40 mg/dL, respectively. Each petri dish contained three drops of maturation medium (300 µl/drops) according to the groups. Microdrops were coated with mineral oil and then incubated in a 5% CO2 incubator, at 39 ËšC with maximum humidity. Aceto-orcein staining was conducted to evaluate the maturation of oocytes based on the achievement of metaphase II phase that is indicated by the presence of metaphase plate and/or first polar body. The result showed that the oocyte maturation rates of P0, P1, and P2 were 51.25, 52.43 (p >0.05), and 46.88 % (p <0.05) respectively. It could be concluded that the presence of urea at 40 mg/dL in maturation medium reduced the percentage of bovine oocyte maturation in vitro.

Efek pemberian α-tokoferol terhadap jumlah sel spermatogenik dan sel Leydig pada tikus putih (Rattus norvegicus) yang dipapar 2,3,7,8 Tetrachlorodibenzo-p-dioxin Machmudia, Ainun; Eliyani, Hana; Widjiati, Widjiati; Wurlina, Wurlina
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 3 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i3.2021.72-80

This research aimed to determine the effect of α-tocopherol on the count of spermatogenic and Leydig cells in rats (Rattus norvegicus) exposed to 2,3,7,8 tetrachlorodibenzo-p-dioxin (dioxin). Dioxin is an endocrine-disrupting agent that adversely affects reproductive health, while α-tocopherol maintains fertility. This research used 25 rats aged 10-12 weeks weighing 150-200 grams. Rats were divided into five groups (K, P0, P1, P2 and P3). The K (control group) was administered with corn oil 1 ml/day. P0 was exposed to 700 ng/kg/day dioxin. P1, P2 and P3 was exposed to dioxin at a dose of 700 ng/kg/day and administered with α-tocopherol at a dose of 77, 140 and 259 mg/kg/day respectively. Dioxin exposure, corn oil and α-tocopherol administration were conducted orally for 20 days. On day-21, all rats were sacrificed for histological slides preparation of testicles with hematoxylin-eosin staining. Data were analyzed with analysis of variance and continued with the Duncan test. The results indicated that exposure to dioxin caused a decrease in the number of spermatogenic and Leydig cells. The administration of α-tocopherol at a dose of 140 mg/kg/day eliminated the effect of reducing the number of spermatogenic and Leydig cells caused by exposure to dioxin. The conclusion was the administration of α-tocopherol at 140 mg/kg/day was effective in maintaining the number of spermatogenic and Leydig cells in rats (Rattus norvegicus) exposed to 2,3,7,8 tetrachlorodibenzo-p-dioxin.

Co-Authors Achmad Salman Faridz, Achmad Salman Agung Budianto Achmad, Agung Budianto Agustina, Widayati Alfiah Hayati Aliyah, Suhartatik Amila, Ukhti Mukminah ilmi Annise Proboningrat Ardy, Carolina Arimbi Askandar Tjokroprawiro Bambang Poernomo Bambang Purwanto Berlina, Cyrcilia Relita Budi Susetyo Pikir Budi Utomo Budi Wicaksono Chairul Anwar Darsono, Roesno Dewita Dewita Dwi Gustin, Mandasari Eighty Mardiyan K, Eighty Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Setyaningsih endra, priawasana Erma Safitri Erry Gumilar Dachlan, Erry Gumilar Gandul Atik Yuliani Hana Eliyani Handini, Haspita Rizki Syurya Hani Plumeriastuti Hariadi, Mas'ud Hendy Hendarto Hermanto Hermanto Hermanto Tri Joewono Hidajati, Nove Hidayatullah, Amiruddin IMAM MUSTOFA INDRA YULIATI, INDRA Iwan Sahrial Hamid Jeanita, Winny Jojor Sihotang Kartikasari, Dhesy Khasanah, Rima Nur Kuncorojakti, Suryo Kurniawati, Sepvian Dewi Lilik Maslachah Luqman, Epy Muhammad Machmudia, Ainun Mafruchati, Maslichah Meles, Dewa Ketut Mulyanto Mulyanto Muninggar, Laili Ngakan Made Rai Widjaja Ningrum, Nyna Puspa Nurbayatin, Anita Nursadida, Milla Nusdianto Triakoso Pardede, Sabrina Melisa Pardede, Sabrina Melisa Paulus Sugianto, Paulus Permatasari, Dian Ayu Poedjo Hartono Poetranto, Emmanuel Djoko Portia Sumarsono, Portia Pudji Srianto Rahadyan, Raihan Alif Rahayu, Pipit Sri Estuning Rahmaniabdi, El Roy Achmad Rao, Sherbrina Bai Seenivasa Relly Yanuari Primariawan, Relly Yanuari Rimayanti Rimayanti RINA YUDIWATI BM, RINA Risna Y, Dyah Ayu Roro Rochmah Kurnijasanti Romi Ermawan, Romi Rusyawardani, Aldis Ingrid Sami, Abdul Sarudji, Suryanie Shabira, Zahra Sri Mulyati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sulistiawati Sulistiawati Suzanita Utama Tias, Eriza Putri Ayu Ning Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Tyasningsih, Wiwiek Viski Fitri Hendrawan Wahyuni, Retno Sri Wicaksono, M Galih Widya Paramita Lokapirnasari Wiranata, Try Ardhi Wurlina, W Yaner, Nurul Ramadhani Yeni Dhamayanti Yudhana, Aditya Yudi Her Oktaviono, Yudi Her Yulianna Puspitasari

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