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Physical and Psychological Stressor Exposure during Pregnancy Impacts the Expression of Synapsin and Neuronal Cells Number of MUS Musculus Offspring Diana Estu Rumahastuti; Hermanto Tri Joewono; Widjiati
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17482

Abstract

Introduction: Stress during gestation can lead to inappropriate fetal brain development, especiallypsychological stress. Psychological stress play pivotal role in offsprings’ brain development.Objective: The aim of this study was to investigate the effects of maternal exposure to physical andpsychological stress during pregnancy on the cerebrum in mice offspring.Method: This study was an analytical experiment study with the subject were 24 female mice Musmusculus. The sample were divided into treatment and control group. Treatment groups divided intothree conditions, 1 group was given physical stress, 1 group with psychological stress, and 1 groupwas given combination of physical and psychological stress intervention. The data were analyzed withANOVA test then followed by LSD to find the differences between all groups.Results: The ANNOVA test results showed significant differences of the expression of synapsin withp = 0.0000. The number of neuronal cells also represented significant differences with p = 0.000 on thecerebrum.Conclusion: Stress exposure during pregnancy can induce bad impacts in brain development, especiallythe expression of synapsin and the number of neuronal cells on the cerebrum in mice offspring.
Steeping Tin Leaves (Ficus carica) Improves Sperm Quality of Male Mice (Mus musculus) Exposed to Lead Acetate Emi Kusumawardani; Budi Santoso; Widjiati; Hari Basuki Notobroto; Heru Santoso Wahito Nugroho
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17490

Abstract

Introduction: Lead can induce lipid oxidation in cell membrans, thus forming free radicals. The process of imbalance of free radicals and antioxidants will disrupt the normal function of cells, causing cell death and decreased sperm quality. Purpose: The purpose of this research is to explain the mechanism of steeping tin leaves (Ficus carica) increase sperm quality in male mice (Mus musculus) exposed to lead acetate. Methods: This type of research was true experimental design with posttest only control group design with the number of replications of 10 male mice, the experimental unit will be distributed proportionally to 5 groups. Steeping tin leaves will be given with a dose of Pb + Tin Leaf 1.664 mg (P1) and Pb + Tin Leaf 3.328 mg (P2), while for lead acetate dose 0.5 mg and quercetin dose 0.7 mg. The analyzed variables included spermatozoa motility, spermatozoa morphology, and spermatozoa concentration. Data analysis was conducted including the Shapiro-Wilks normality test, and the homogeneity test used the Levene test. If the data were not homogeneous, the group average test would use the Brown-Forsythe test, then continued with a different test for each group using the Post Hoc Games-Howell test. If the Levene test data were homogeneous, the group average test would use Oneway Anova. Findings: The results showed that giving of steeping tin leaves with a dose of Pb + Tin leaves 3.328 mg (P2) is able to increase spermatozoa motility and spermatozoa morphology. Conclusion: The steeping tin leaves increase sperm quality
The Effect of Adiponectin Recombinant in Rattus Norvegicus with Polycystic Ovarium Syndrome Model on Anti-Müellerian Hormone Expression Joseph Chandra Relmasira; Jimmy Yanur Annas; Widjiati
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17570

Abstract

Background: Polycystic Ovary Syndrome (PCOS) is an important disease that causes various healthproblems in women. Ovulation - infertility disorders are found in 60-85% woman with PCOS. Anti-Muellerian Hormone (AMH), a protein from Transforming Growth-b Family, often increased in patientwith PCOS and associated with severity and poor prognosis for assisted reproductive outcome. Dietaryand lifestyle changes are the first-line therapies but consistent application of this method is difficult toattain for people with PCOS. Adiponectin, a product by adipose cells have a significant reduction inobese and women with PCOS. The purpose of this study was to see the differences in AMH expressionbetween mice with polycystic ovary model treated with adiponectin recombinant and mice withpolycystic ovary model treated with placebo and control.Method: This research is an experimental study with a Post Test Only Control Group design. Thesample of this study was 3 months female Rattus norvegicus Wistar strain weighing 110-120 g. Thesample size was 36, which were divided into three groups, K0 (control group of mice with polycysticovary model), K1 (group of mice with polycystic ovary model that received placebo injection), and K2(group of mice with polycystic ovary model that received 5 μg/ml injection of recombinant adiponectin).Polycystic ovary model made by injecting 10 mg/Kg body weight testosterone propionate for 14 days.The injection is carried out at the proestrous stage. The three groups of mice in a period of 3 weeks aftertreatment, were surgically removed according to the sampling procedure, then the ovaries were given acode and immunohistochemical staining was performed to see the AMH expression in the ovaries. Thedata were collected, then statistical analysis was carried out using SPSS software.
Comparison of Akt Expression in the Cerebrum and Cerebellum of Newborn Mus musculus Exposed to Physical Stress and Psychological Stress During Pregnancy Herlina Puji Angesti; Hermanto Tri Joewono; Widjiati
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.18064

Abstract

Introduction: Prenatal stress prevalence is almost half of the population of pregnant women worldwide.Stress will stimulate glucocorticoids which can disrupt the PI3K-Akt cascade. PI3K-Akt deficiency willcause impaired fetal brain growth and development.Objective: To compare the Akt expression in the cerebrum and cerebellum of newborn Mus musculusexposed to physical stress, psychological stress, the combination of psychological and physical stress, andwithout stress exposure. Method: This study was experimental laboratory research. Twenty-four female mice were used as samplesand divided into four groups: physical stress exposure group, psychological stress exposure group, thecombination of psychological and physical stress exposure group, and control group. Akt expression wastested by immunohistochemistry (IHC) staining. Statistical analysis used the One Way Anova and KruskalWallis test.
Isolation and Identification of Transforming Growth Factor β from In Vitro Matured Cumulus Oocyte Complexes . WIDJIATI; ARIEF BOEDIONO; SUTIMAN BAMBANG SUMITRO; AUCKY HINTING; . AULANI’AM; TRINIL SUSILOWATI
HAYATI Journal of Biosciences Vol. 19 No. 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (92.482 KB) | DOI: 10.4308/hjb.19.1.6

Abstract

Transforming growth factor-β (TGF-β) is a two-chain polypeptide with molecular weight of 25 kDa which takes significant role in the steroidogenesis process. In the ovarian oocyte in particular, TGF-β has an important role in regulating reproductive function. TGF-β represents a key intrafollicular protein that regulates follicle development and aromatization process. The purpose of this research was to characterize and identify a protein fraction of TGF-β from the bovine isolated oocytes, which is synthesized during in vitro oocyte maturation process. Oocytes were collected from follicles with diameter of 3-8 mm. Oocytes were then matured in TCM 199 media supplemented with 5 μg/mg LH, 3% BSA, and 50 μg/ml gentamicin sulfate, and cultured in CO2 incubator (5%, 38.5 oC) for 20 hours. TGF-β receptors were identified immunohistochemically. Characteristics of the TGF-β protein were determined using SDS PAGE and TGF-β specification was tested using Western Blotting. The results showed that TGF-β receptors were identified and found in cumulus oocyte complexes (COCs). TGF-β protein was isolated from bovine oocytes with molecular weight 25 kDa and it was identified by Western blotting methods in the same molecular weight.
Calorie Restriction and Moderate-Intensity Continuous Exercise Decrease Free Fatty Acid Levels and Visceral Fat Weight on High Calorie Diet Female Mice Ahmad Rukhani Lutfi; Lilik Herawati; Widjiati; Gadis Meinar Sari
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14944

Abstract

Objective: This study aimed to analyze the effect of a combination of calorie restriction and moderateintensity continuous exercise on FFA levels and visceral fat weight in female mice exposed to a high-caloriediet.Material and Method: This research was randomized posttest used only control group design with femalemice as the sampl Balb/c mice (Mus musculus), 8 weeks old, 20-30 grams. It was randomly divided into fourgroups, namely C (control group), G1 (moderate-intensity continuous exercise group), G2 (calorie restrictiongroup), and G3 (combination group calorie restriction and moderate-intensity continuous exercise) wherein every group consisted six female mice. Providing a high-calorie diet using ad libitum plus D40 as muchas 3-5% of calories (0.0325 mL/gBB) for 4 weeks. The provision of a high-calorie diet was done usingthe sonde technique. Calorie restriction was done 3x/week in 4 weeks by limiting calorie intake to 50% ofstandard foods. The moderate-intensity continuous exercise was carried out by swimming for 15 minutesplus 6% gBB loading, it was done 3 times/week for 4 weeks. The measurement of cholesterol, visceral fatweight, and FFA level was done at the end of treatments.Results: The results found that the mean of cholesterol of C C (171.00±47.74 mg/dL), G1 (126.00±15.92 mg/dL), G2 (131.50±22.37 mg/dL), G3 (121.17±21.23 mg/dL) dan (p=0.032). The mean weight of visceral fatat C (0.68±0.23 grams), G1 (0.25±0.08 grams), G2 (0.28±0.06 grams), G3 (0.20±0.03 grams) and (p=0.000).The mean FFA levels were at C (1511.34±6.19 µmol/L), G1 (1419.31±53.61 µmol/L), G2 (1458.20±28.89µmol/L), G3 (1451.55±43.77 µmol/L) and (p=0.004).Conclusion: Based on the results of the study, it could be concluded that the provision of calorie restrictionand moderate-intensity continuous exercise significantly reduced cholesterol levels, visceral fat weight, FFAlevels and in female mice exposed to a high-calorie diet
Effect of Cacao on Plasma F2-Isoprostane Level, CD34 and ICAM-1 Expression of Coronary Arteries in Cigarette Smoking Exposed Rats Dina Helianti; Soetjipto; Widjiati; I Ketut Sudiana
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15729

Abstract

This research aimed to evaluate cardioprotective effects of cacao in smoking exposure condition that the effects were mediated through the anti-oxidant pathway by measure plasma F2-isoprostane level, Endothelial Progenitor Cell (EPC) enhancement by expression of CD34, while dysfunction endothelial condition was measured by expression of ICAM-1 in coronary arteries. This study subjected rats, divided into four groups: the normal control group (2 ml of aqua bidest, air exposure); the cigarette control group (2 ml of aqua bidest, cigarette smoke); cacao group 1 (1205 mg/kg BW/day, cigarette smoke); cocoa group 2 (2410 mg/kg BW/day, cigarette smoke). The oxidant biomarker, F2-isoprostane level was assessed using ELISA; CD34, and ICAM-1 expression in coronary arteries by immunohistochemistry. Cacao 1205 mg/kg BW/day significantly decreases plasma F2-isoprostane level, and ICAM-1 expression of coronary arteries in cigarette smoking exposed rat (p < 0.05) but there was not a significant increases CD34 (p < 0.05). Cocoa in cigarette smoke-exposed rats can prevent endothelial dysfunction through decrease F2-isoprostane but not increase CD34. The results of this study can be used as a basis for preventing endothelial dysfunction due to cigarette smoke by using cacao.
Jumlah Sel Neuron Cerebrum pada Paparan Musik Mozart Lebih Tinggi Dibandingkan Dengan Paparan Musik Indonesia Agustina Mar&#039;atus Sholichah; Hermanto Tri Joewono; Widjiati Widjiati
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 11, No 1 (2020): Januari 2020
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf11104

Abstract

Background: Intelligence management can produce superior human resources with integrated brain. Mozart music stimulus during pregnancy has been shown to increase the number of neuron of the fetal brain. The study of pop and religious music needs to be improve because they are more popular in Indonesia. Objective: To analyze the differences of number of Neuron in the Cerebrum Rattus norvegicusoffspring that exposed to Mozart, Indonesian pop music and Indonesian religious music during pregnancy. Methods: An experimental study with a post-test only control group design. Groups divide into treatment music groups: Mozart, pop and religious. Treatment in a soundproof room for 1 hour, starting the 10th-day of pregnancy, intensity of 65 dB with a distance of 25 cm from the cage. The number of neuron was counted from HE brain preparations of the head Rattus norvegicusoffspring and analyzed using appropriate statistics test. Results: There were significant differences in the number of neuron of Rattus norvegicusoffspring in cerebrum between groups with p = 0,000 (mean Mozart music group 28.14 ± 3.02, Indonesian pop music 19.71 ± 1.80, Indonesian religious music 24.14 ± 2.91) and Mozart gave a higher number of neuron than Indonesia religious music and Indonesian pop music. Conclusion: Mozart music gave a higher number of neuron in the Cerebrum than Indonesian religious music and Indonesian pop music. Keywords: neuron; cerebrum; Mozart music; Indonesian music ABSTRAK Latar belakang: Pengelolaan kecerdasan otak yang terintegrasi akan menghasilkan SDM yang unggul. Stimulus musik Mozart selama kehamilan terbukti meningkatkan jumlah sel neuron di otak janin. Musik pop dan religi perlu dilakukan penelitian karena lebih populer di Indonesia. Tujuan: Menganalisis perbedaan jumlah sel neuron di Cerebrum Rattus norvegicusbaru lahir antara yang mendapat paparan musik Mozart, musik pop Indonesia dan musik religi Indonesia selama kebuntingan. Metode: Studi eksperimental dengan desain post test only control group. Kelompok perlakuan dibagi menjadi kelompok musik Mozart, musik pop Indonesia dan musik religi Indonesia. Perlakuan di ruang kedap suara selama 1 jam pada malam hari mulai hari ke-10 kebuntingan, intensitas 65 dB dengan jarak 25 cm antara kandang dan speaker. Jumlah sel neuron dihitung dari preparat pewarnaan Hematoxylin-Eosin otak anak Rattus norvegicusdan dianalisis dengan statistik yang sesuai. Hasil: Terdapat perbedaan yang signifikan pada jumlah sel neuron cerebrum Rattus norvegicus baru lahir antar kelompok dengan nilai p=0,000 (rerata kelompok musik Mozart 28,14±3,02, musik pop Indonesia 19,71±1,80 dan musik religi Indonesia 24,14±2,91) dan musik Mozart memiliki jumlah sel neuron lebih tinggi daripada musik religi Indonesia dan musik pop Indonesia. Kesimpulan: Kelompok musik Mozart memiliki jumlah sel neuron di cerebrum yang lebih tinggi dibandingkan kelompok musik Indonesia. Kata kunci: neuron; cerebrum; musik Mozart; musik Indonesia
Mozart Memberikan Hasil Indeks Apoptosis Lebih Rendah daripada Musik Pop, Religi dan Tanpa Pemberian Musik Dian Sukmawati; Hermanto Tri Juwono; Widjiati Widjiati
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 11, No 1 (2020): Januari 2020
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf11106

Abstract

Background: Brain growth requires genetic potential for a conducive environment, low stress levels, stimulation and nutrition. Brain development during the fetal period in the two years of life firstly requires proper stimulation to increase intelligence. Classical music stimulation has been proven to be able to optimally improve brain function and human intellect. Other music that Indonesian people like is pop and religion. Maybe it is possible to pop and religious music can affect the development and growth of the fetal brain. Objective: Analyze the differences of the index of apoptosis Cerebrum and Cerebellum Rattus norvegicus newborn between those stimulated by Mozart, pop, religious music and not stimulated by music during pregnancy. Method: This study was post test only control group design. groups were randomly divided according to the treatment as stimulation of Mozart, pop, religious music and no music was given from the 10th day-gestation with an intensity of 65 dB in a soundproof box for one hour. When 19th day-gestation, Rattus norvegicus mothers were sacrificed then 3 Rattus norvegicus newborn from each mothers were selected and brain was taken to be made immunohistochemical preparations and counted the number of neuron cells apoptosis index. data were analyzed by comparison test with p
Penetapan Total Flavonoid, Aktivitas Antioksidan dan Karakterisasi Nanopartikel Ekstrak Etanol Daun Kelor (Moringa pterygosperma Gaertn.) Tinta Julianawati; Hendy Hendarto; Widjiati Widjiati
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 11, No 1 (2020): Januari 2020
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf11110

Abstract

Moringa pterygosperma is one of the plants in Indonesia that is often used in herbal medicine. Moringa leaves contain many phytochemical compounds, one of the compounds is flavonoids which function as antioxidants. Flavonoid compounds in vitro can inhibit lipid peroxidation by breaking the chain of peroxyl radicals. Moringa leaf benefits in the field of herbal medicine are formed with a variety of preparations. One of the preparations is in the form of extracts with the aim of attracting chemical components to natural materials The extraction process has a disadvantage because the level of solubility in water is still low. The nanoparticle technique is a strategy to increase the bioavalbicibility of herbal active compounds. This research was experimental in order to determine the total flavonoid levels contained in Moringa leaf extract. In addition, it was also to find out the antioxidant activity and particle size of the moringa leaf extract nanoparticles. Determination of flavonoid levels using spectrophotometric methods. The antioxidant activity of moringa leaf extract nanoparticles was tested using the DPPH method (2,2-diphenyl-picrylhdrazyl) and to determine the particle size used by using the Particle Size Analyzer (PSA). The results of the study showed total flavonoid levels in moringa leaf extract (1.97 ± 1.07)%. Antioxidant activity of 451.8 ppm with a reduction of 2.8% and particle size in the moringa leaf extract nanoparticles of ± 134.78 nm. Moringa leaf extract nanoparticles include low antioxidants. Keywords: moringa; nanoparticles; antioxidants
Co-Authors . AULANI’AM Achmad Salman Achmad Salman Faridz, Achmad Salman Achmad Yunus Adi Setyawan Prianto Agung Budianto Achmad, Agung Budianto Agus Sulistyono Agustina Hidayati Agustina Mar&#039;atus Sholichah Agustina, Widayati Ahmad Rukhani Lutfi Alfiah Hayati Ali Madinah Ali Medina Ali Medina Aliyah, Suhartatik Amila, Ukhti Mukminah ilmi Ancha Ayu Amishinta Andita Hapsari Anike Rachmawati Anike Rachmawati Anindya Marcha Angelina Djianto Anna Surgean Veterini Annis Catur Adi Annise Proboningrat Anwar Fauzi Ardy, Carolina Arief Boediono Arimbi Askandar Tjokroprawiro Aswin, R. Haryanto Aty Widyawaruyanti Aucky Hinting Ayu Putri Yani Azami D Azinar Bambang Poernomo Bambang Purwanto Bambang Sektiari Lukiswanto Bambang Wahjuprajitno Berlina, Cyrcilia Relita Binta Dwi Novitasari Boedi Setiawan Budi I Santoso Budi Prasetyo Budi Santoso Budi Santoso Budi Santoso Budi Susetyo Pikir Budi Susetyo Pikir Budi Utomo Budi Utomo Budi Wicaksono Budi Wicaksono Chairul Anwar Darsono, Roesno Desak Ketut Ayu Aryani Desi Wulansari Desi Wulansari Dessy Hidayati Fajrin Dewita Dewita Dhesy Kartikasari Dian Sukmawati Diana Estu Rumahastuti Dina Helianti Dwi Gustin, Mandasari Dwi Nurdi Puspita Sari Dwi Puji Wijayanti Eighty M Kurniawati Eighty Mardiyan K, Eighty Eka Nasrur Maulana Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Setyaningsih endra, priawasana Epy Muhammad Luqman Erma Safitri Ermawati Ermawati Erry Gumilar Dachlan, Erry Gumilar Dachlan, Erry Gumilar Exma Mu&#039;tatal Hikmah Faizah, Zakiyatul Fauzan Djunaidi Fedik A Rantam Fedik Abdul Rantam Fitria Desky Gandul Atik Yuliani Gatut Hardianto, Gatut H.M Bahkriansyah Hamdani Lunardhi Hana Eliyani Hani Plumeriastuti Hany Aryani Puspita Hari Basuki Notobroto Hari Paraton Hariadi, Mas'ud Harry Mangasi Binsar Panjaitan Haspita Rizki Syurya Handini Hendy Hendarto HENI RACHMAWATI HERAWATI, LILIK Herlina Alvianti N Herlina Puji Angesti Hermanto Hermanto Hermanto Hermanto Hermanto T Joewono Hermanto TJ Hermanto Tri J Hermanto Tri Joewono Hermanto Tri Juwono Heru Santoso Wahito Nugroho Hidajati, Nove Hidayatullah, Amiruddin Ika WAHYUNI IMAM MUSTOFA Imam Mustofa INDRA YULIATI, INDRA Inu Mulyantoro Iwan Sahrial Hamid Jeanita, Winny Jimmy Yanur Annas Jojor Sihotang Joseph Chandra Relmasira Kartikasari, Dhesy Khasanah, Rima Nur Kuncorojakti, Suryo Kurniawati, Sepvian Dewi Kusumawardani, Emi Laba Mahaputra Laili Muninggar Laksmi Sulmartiwi Lilik Maslachah Machmudia, Ainun Mafruchati, Maslichah Masyitah Hamidah Meitria Syahadatina Noor Meles, Dewa Ketut Mikra Latisfian Mikra Latisfian Mikra Latisfian Muhammad Miftahussurur Mulyanto Mulyanto Mulyanto Mulyanto Muninggar, Laili MY Ardianta W N Cininta M Nasrul Wahdi Nenny Harijani Ngakan Made Rai Widjaja Ngakan Made Rai Widjaja Ningrum, Nyna Puspa Noor Pramono Noor Pramono Noor Pramono Noor Pramono Noerpramana Nur Anindya Syamsudi Nur AR Widiatmoko Nur Cholila Nur Laila Faizah Nurbayatin, Anita Nursadida, Milla Nurvy Alief Aidillah Nusdianto Triakoso Nyna Puspa Ningrum Oktoria Indrapraja Pardede, Sabrina Melisa Pardede, Sabrina Melisa Paulus Liben Paulus Sugianto, Paulus Permatasari, Dian Ayu Pipit Sri Estuning Rahayu Pitria Permatasari Poedjo Hartono Poetranto, Emmanuel Djoko Portia Sumarsono, Portia Pudji Srianto Rachmad Suhanda Rahadyan, Raihan Alif Rahayu, Pipit Sri Estuning Rahmaniabdi, El Roy Achmad Rao, Sherbrina Bai Seenivasa Ratriana Via Parasti Raudatul Hikmah Rehatta, Nancy Margarita Relly Yanuari Primariawan, Relly Yanuari Rimayanti R Rimayanti R, Rimayanti Rimayanti Rimayanti RINA YUDIWATI BM, RINA Risna Y, Dyah Ayu Roro Rochmah Kurnijasanti Romi Ermawan Romi Ermawan, Romi Rusyawardani, Aldis Ingrid S.Pd. M Kes I Ketut Sudiana . Salmon Charles Pardomuan Tua Siahaan Sami, Abdul Sari, Gadis Meinar Sarmanu, Sarmanu Sarudji, Suryanie Seindy Glamour Shabira, Zahra Siti Nur Kholifah Siti Nur Kholifah Siti Nur Kholifah SOETJIPTO Sofiyanti Miftakhurohmah Sri Mulyati Sri Mulyati Sri Mumpuni Sri Mumpuni Sri Pantja Madyawati Sri Pantja Madyawati, Sri Sri Pantja Madyawati, Sri Pantja Sri Ratna Dwiningsih Subijanto Marto Soedarmo Suherni Susilowati Sulistiawati Sulistiawati Sutiman Bambang Sumitro Suwarno . Suzanita Utama Syarief T. Hidayat Syarief Thaufik Hidayat Syifa Fauziyah Tias, Eriza Putri Ayu Ning Tinta Julianawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Hartini Yuliawati Tri HS Hadi Tri Purwanti Tri Wahyu Suprayogi Trilas Sardjito TRINIL SUSILOWATI Tyasningsih, Wiwiek Ukhti Mukminah ilmi Amila Varianida Veterini Virki Widoyanti Viski Fitri Hendrawan Wahyuni, Retno Sri Wicaksono, M Galih Widodo Jatim P Widya Paramita Lokapirnasari Windhu Purnomo Wiranata, Try Ardhi Wurlina, W Yaner, Nurul Ramadhani Yeni Dhamayanti Yoes Priyatna Dachlan Yudhana, Aditya Yudi Her Oktaviono Yudi Her Oktaviono, Yudi Her Yuli Trisetiyono Yulia Putri Permatasari Yulianna Puspitasari