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All Journal Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Folia Medica Indonesiana Indonesian Journal of Cancer Universa Medicina Journal Of Vocational Health Studies Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Jurnal Medik Veteriner journal of Basic Medical Veterinary Current Biomedicine
Widjiati Widjiati, Widjiati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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EFEK PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL LEYDIG MENCIT (Mus musculus) JANTAN YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN Nursadida, Milla; Plumeriastuti, Hani; Dhamayanti, Yeni; Widjiati, Widjiati; Luqman, Epy Muhammad; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19998

Abstract

This study was aim to examine the effect of kebar grass extract (Biophytum petersianum Klotzsch) to against number leydig cells of mice (Mus musculus) by exposed 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD). Twenty five male mice (Mus musculus) 4 months with avarage body weight 20 g were used. These animals were divided into five groups (K(-), K(+), P1, P2 and P3). K(-) was treated with placebo, K(+) was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw, P1 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,045 mg/g Bw/day P2 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,080 mg/g Bw/day, P3 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 1,350 mg/g Bw/day. This research has been conducted for 53 days. The data were compared using ANOVA and Duncan test by SPSS 22.4 for windows. The result showed that Kebar Grass Extract in all of groups can prevent the damage of leydig cells in testis that exposed by TCCD significantly (p<0,05) and kebar grass extract 0,135 mg/kgBw/day can increase amount of leydig cells maximaly.
PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP GAMBARAN HISTOPATOLOGI BRONKUS DAN VENA PULMONALIS MENCIT (Mus musculus) JANTAN YANG DIPAPAR ASAP ROKOK Jeanita, Winny; Meles, Dewa Ketut; WIdjiati, Widjiati; Hamid, Iwan Sahrial; Luqman, Epy Muhammad; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21089

Abstract

This research aimed to attest the effect of Kepok banana (Musa acuminata) peel extract on the histopathology of bronchus and pulmonary veins in male mice (Mus musculus) exposed by cigarette smoke. The 24 experimental male mice were divided into six groups with each treatment having four replicate. All the groups, except K- as a control group, was treated with cigarette smoke for 14 days. After that, K- and K+ was treated with CMC-Na 1%, KO was treated with ascorbic acid 13 mg/kgBW, P1 was treated with Kepok banana peel extract 14 mg/kgBW, P2 was treated with Kepok banana peel extract 28 mg/kgBW, and P3 was treated with Kepok banana peel extract 56 mg/kgBW for 14 days. The data of the thickness of bronchial epithelium and the diameter of pulmonary veins were analyzed using ANOVA (Analysis of Varience) with significance p<0,05 and continued with Post- hoc Tukey. The result of the thickness of bronchial epithelium showed that K+ had the highest thickening (431,83 + 9,71 µm) and significant differences (P<0,05) with all the groups. The result of P3 showed the lowest thickening of bronchial epithelium (170,84 + 2,70 µm). The result of pulmonary veins diameter showed that K+ had the narrowest diameter (525,64 + 16,61 µm) and significant differences (p<0,05) with all the groups. The result of P3 showed the lowest diameter narrowing of pulmonary veins (718,10 + 9,64 µm). The conclussion of this research are Kepok banana peel extract able to decreased the thickness of bronchial epithelium and the narrowing of pulmonary veins diameter in male mice with effective dose 28 mg/ kgBW.
STEM CELL THERAPY ON ANIMAL MODEL (Rattus norvegicus) END GESTATIONAL TIME EXPOSED TO CARBON BLACK AS OBSERVED FROM CASPASE-3 EXPRESSION Achmad, Agung Budianto; Madyawati, Sri Pantja; Widjiati, Widjiati
Journal of Vocational Health Studies Vol. 1 No. 3 (2018): March 2018 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V1.I3.2018.102-106

Abstract

Background: Air pollution in the form of Diesel Exhaust Particles emerging from motor vehicles are harmful to health that have adverse reproductive health impacts, especially during pregnancy. The use of stem cells in treating white mice (Rattus norvegicus) exposed to carbon black can reveal the potential for treatment of placental impairment during pregnancy. Purpose: to demonstrate the effectivity of Rat Bone Marrow Mesenchymal Stem Cell therapy on rats (Rattus norvegicus) exposed to carbon black as observed from caspase-3 expression. Method: This research uses a completely randomized design with factorial pattern. Forty-eight gravid female rats were divided into six treatment groups. Result: caspase-3 expression in each treatment showed no significant differences in the groups treated with RBMMSC in each gravid groups treatment (therapy GD 11 and GD 17) were exposed to carbon black and not treated with RBMMSC. The same are indicated by the normal trophoblast cells (cytotrophoblast and syncytiotrophoblast cells) in the RBMMSC treated group showed no significant difference with the group exposed to carbon black only. Conclusion: this research indicate that Rat Bone Marrow Mesenchymal Stem Cell therapy in Rattus norvegicus exposed to carbon black have not been able to reduce expression of caspase-3.
Impact of Freeze-Dried Amnion Membrane and Human Amnion Stem Cell Seeding on TGF-B and Collagen Type III in Vesicovaginal Fistula Sihotang, Jojor; Mardiyan, Eighty; Widjiati, Widjiati; Hidayatullah, Amiruddin
Indonesian Journal of Obstetrics and Gynecology Volume 12 No. 4 October 2024
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32771/inajog.v12i4.2139

Abstract

Objective: to analize how freeze-dried amniotic membrane and human amniotic stem cell seeding affect TGF B and type III collagen expression in suturing a New Zealand rabbit vesicovaginal fistula model. Metode: This experimental study employed New Zealand rabbits and a vesicovaginal fistula model with a post-test only control group design. The rabbits were divided into 3 groups: vesicovaginal fistula suturing alone, suturing with freeze-dried amniotic membrane, and suturing with freeze-dried amnion-seeded stem cells. After 7 days of treatment, specimens near the repaired vesicovaginal fistula were collected for immunohistochemical analysis of TGF B and collagen type III expression. Result: TGF B expression was significantly higher in the freeze-dried amniotic membrane with stem cell seeding group (p=0.001) compared to the freeze-dried amniotic membrane without stem cell seeding group (p=0.017) and the suturing-only group (p=0.049). Additionally, type III collagen expression was significantly elevated in the freeze-dried amnion membrane and stem cell seeding group (p=0.001) compared to the freeze-dried amnion group without stem cell seeding (p=0.09) and the suturing-only group (p=0.026). Conclusion: The expression of TGF B and type III collagen was higher in rabbits with vesicovaginal fistulas treated using freeze-dried amnion and amniotic stem cell seeding compared to those without amniotic stem cell seeding and vesicovaginal fistula suturing alone. Keywords: Vesicovaginal Fistula, Freeze-dried Amnion, Stem cell
Pengaruh Pemberian Nanocurcumin (Curcuma longa) Terhadap Gambaran Histopatologis Pulmo Tikus (Rattus norvegicus) Yang Dipapar Carbon Black Rahmaniabdi, El Roy Achmad; Mustofa, Imam; Hestianah, Eka Pramyrtha; Widjiati, Widjiati; Kurnijasanti, Rochmah; Plumeriastuti, Hani
Current Biomedicine Vol. 3 No. 1 (2025): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.1.38

Abstract

Background Exposure to carbon black can induce respiratory toxicity, leading to tissue remodeling, inflammation, and fibrosis. Curcumin, a compound from Curcuma longa, has been shown to exert protective effects on lung tissue by inhibiting cell proliferation and reducing alveolar epithelial thickening and inflammatory responses. Objective This study aimed to evaluate the effect of nanocurcumin administration on alveolar septal thickness and fibrosis in the lungs of rats (Rattus norvegicus) exposed to carbon black. Methods Thirty female rats were divided into five groups: the negative control group (K-) received distilled water without carbon black exposure; the positive control group (K+) received distilled water and was exposed to carbon black at a dose of 1064 mg/m³ for 6 hours; and treatment groups P1, P2, and P3 received nanocurcumin at doses of 50, 100, and 200 mg/kg body weight, respectively, along with carbon black exposure at a dose of 1064 mg/m³ for 6 hours/day over 30 days. Results Alveolar septal thickness and fibrosis in the P1, P2, and P3 groups showed significant reductions (P<0.05) compared to the K+ group. Notably, groups P2 and P3 exhibited similar alveolar septal thickness to the K- group, with statistically lower (P<0.05) fibrosis levels than P1 but not significantly different from K- (P>0.05). Conclusion Nanocurcumin administration demonstrates potential in mitigating alveolar septal thickening and fibrosis in rat lungs subjected to carbon black exposure, suggesting a protective role against pulmonary damage from particulate matter.
RADIATION EFFECT OF WIRELESS FIDELITY (Wi-Fi) ON OOCYTE NUMBER OF OOCYTE STIMULATION IN MICE (Mus Musculus) Nurbayatin, Anita; Widjiati, Widjiati; Primariawan, Relly Yanuari; Poernomo, Bambang; Sulistiawati, Sulistiawati; Yudiwati, Rina
Folia Medica Indonesiana Vol. 53 No. 3 (2017): September 2017
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.449 KB) | DOI: 10.20473/fmi.v53i3.6440

Abstract

Infertility is a problem experienced by some women and men around the world. Most infertility problems in women is caused by impaired reproductive organs or disrupted ovulation.. One factor that causes impaired oocyte maturation is wireless fidelity (Wi-Fi) radiation which has a radio frequency field of 2.45 GHz. The radiation may increase the activity of free radical cells through the fenton reaction pathways that cause infertility because of the disrupted oocyte development. This study aimed to determine the effect of Wi-Fi radiation on the number of oocytes. This was an experimental study using control group design. The subjects were 32 female mice selected through complete random sampling and divided into two groups: control (R0) and exposure (R1). Each group consisted of 16 mice. The Wi-Fi radio frequency used was 2.5 GHz. Mice (R1) were placed closer to Wi-Fi source (± 15cm), and there were two types of laptop PCs and 3G mobile phones connected to internet placed next to mice for 15 hours/28 days. The mice underwent a simultaneous cycle with intraperitoneal injection of PMSG and HCG. Furthermore, the mice were mated with vasectomized male monomatingly to induce ovulation. The fertilization pouch in both oviducts were observed for oocyte collection. The number of oocytes was calculated using an inverted microscope. There was a difference in the number of oocytes between control and exposure group. Statistical tests were analyzed using Mann Whitney U and resulted in significant values (p value = 0.00). No oocytes count in exposure group. In other words, the group underwent anovulation. In conclusion, Wi-Fi radiation affected the number of oocyte stimulation in mice. Therefore, it was important to minimize the risk factors that trigger electromagnetic radiation on reproductive health.
The Effect of Methylmercury Exposure on Astrocyte of Cerebellar Cortex of White Rats (Rattus novergicus) Sugianto, Paulus; Pardede, Sabrina Melisa; Widjaja, Ngakan Made Rai; Widjiati, Widjiati
Folia Medica Indonesiana Vol. 55 No. 2 (2019): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.194 KB) | DOI: 10.20473/fmi.v55i2.24598

Abstract

The aim of this research was to investigate the effect of different dose methylmercury (II) chloride on astrocyte in cerebellar cortex of white rat (Rattus norvegicus) exposed. This study used randomized control design using 15 adult female Wistar rats weight 180-200 g of body weight. Before treatment the white rats was adapted in a week, then randomly divided into 3 groups each consist of 5 rats. P0 as control were given 0.5 ml aquades, P1 and P2 were given 0.1 and 0.2 mg/kg/day respectively. All groups were given treatment per oral in 30 days with sonde. The data was analyzed by ANOVA, Duncan's multiple range (Duncan's Multiple Range Test). White rats exposed by methylmercury (II) chloride, had a significant differences in the percentage of necrotic astrocyte (p<0.05). Methylmercury chloride exposure increases the number of necrotic astrocytes on white rat.
Role of Cryoprotectants in Preventing Quality Loss of Goat Oocytes after Warming by Examining Insulin-Like Growth Factor-1 and Bone Morphogenetic Protein-15 Expression Shabira, Zahra; Widjiati, Widjiati; Rimayanti, Rimayanti; Lestari, Tita Damayanti; Safitri, Erma; Mafruchati, Maslichah; Hendrawan, Viski Fitri
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.175-182

Abstract

This research aimed to assess the efficacy of ethylene glycol-sucrose as an alternative cryoprotectant compared to commercial cryoprotectants by focusing on the expression of insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-15 (BMP-15) in goat oocyte cumulus complexes after warming with identification using the immunocytochemical analysis. Maturated oocytes were divided into 3 groups, i.e., (C) the control group followed by an immunocytochemical examination, and two treatment groups, i.e., (P1) was exposed to a commercial cryoprotectant for 15 minutes, then placed in a hemistraw and dipped in liquid nitrogen, and (P2) was exposed to 30% ethylene glycol and 1 M sucrose for 15 minutes, then placed in a hemistraw and dipped in liquid nitrogen. The results revealed divergent responses in IGF-1 (CG = 9.00 ± 3.00; P1 = 9.50 ± 3.20; P2 = 4.67 ± 0.94) and BMP-15 (CG = 10.50 ± 3.35; P1 = 9.50 ± 2.69; P2 = 5.50 ± 3.64) expression patterns, possibly influenced by the two cryoprotectant abilities and oocytes performing their permeabilities to cryoprotectant solution. We concluded that each cryoprotectant is necessary to mitigate the adverse impact on gene expression in oocytes so that we can use it for reproductive technologies.
Co-Authors Achmad Salman Faridz, Achmad Salman Agung Budianto Achmad, Agung Budianto Amila, Ukhti Mukminah ilmi Annise Proboningrat Ardy, Carolina Arimbi Askandar Tjokroprawiro Bambang Poernomo Berlina, Cyrcilia Relita Budi Wicaksono Chairul Anwar Dewita Dewita Eighty Mardiyan K, Eighty Eka Pramyrtha Hestianah, Eka Pramyrtha Erma Safitri Erry Gumilar Dachlan, Erry Gumilar Gandul Atik Yuliani Hana Eliyani Hani Plumeriastuti Hariadi, Mas'ud Hermanto Hermanto Hermanto Tri Joewono Hidajati, Nove Hidayatullah, Amiruddin IMAM MUSTOFA INDRA YULIATI, INDRA Iwan Sahrial Hamid Jeanita, Winny Jojor Sihotang Kuncorojakti, Suryo Lilik Maslachah Luqman, Epy Muhammad Mafruchati, Maslichah Meles, Dewa Ketut Muninggar, Laili Ngakan Made Rai Widjaja Nurbayatin, Anita Nursadida, Milla Nusdianto Triakoso Pardede, Sabrina Melisa Pardede, Sabrina Melisa Paulus Sugianto, Paulus Permatasari, Dian Ayu Poedjo Hartono Poetranto, Emmanuel Djoko Portia Sumarsono, Portia Rahadyan, Raihan Alif Rahayu, Pipit Sri Estuning Rahmaniabdi, El Roy Achmad Rao, Sherbrina Bai Seenivasa Relly Yanuari Primariawan, Relly Yanuari Rimayanti Rimayanti Rochmah Kurnijasanti Rusyawardani, Aldis Ingrid Sami, Abdul Shabira, Zahra Sri Mulyati Sri Pantja Madyawati, Sri Pantja Suherni Susilowati Sulistiawati Sulistiawati Suzanita Utama Tita Damayanti Lestari Tri Wahyu Suprayogi Tyasningsih, Wiwiek Viski Fitri Hendrawan Wahyuni, Retno Sri Widya Paramita Lokapirnasari Wiranata, Try Ardhi Yeni Dhamayanti Yudhana, Aditya Yudiwati, Rina Yulianna Puspitasari