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All Journal Tropical Medicine Journal Scriptura Jurnal Pendidikan Kimia Intuisi Journal of Mathematical and Fundamental Sciences INKUIRI : Jurnal Pendidikan IPA Jurnal SOLMA Jurnal Ilmiah Manuntung Jurnal Ilmiah Sekolah Dasar Walisongo Journal of Chemistry Jurnal Pembelajaran Sains Jurnal Inovasi Pendidikan dan Sains Panrannuangku Jurnal Pengabdian Masyarakat Student Scientific Creativity Journal Jurnal Ilmiah Pengabdian dan Inovasi Room of Civil Society Development Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education) Morfologi : Jurnal Ilmu Pendidikan, Bahasa, Sastra dan Budaya Bulletin of Chemical Reaction Engineering & Catalysis Indonesian Journal of Biology Education Asian Journal of Environmental Research Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan Room of Civil Social Development
Dian Nugraheni
Universitas Negeri Malang
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : Bulletin of Chemical Reaction Engineering

 1 
Extracellular Lipase from Pseudomonas aeruginosa SB-37: Production by Solid State Fermentation, Immobilization, and Characterization Haryati, Titin; Haryono, Norman Yoshi; Nugraheni, Dian; Fatmawati, Fenti; Dewi, Alfina Kusuma; Syach, Muhammad Zahran Edlian
Bulletin of Chemical Reaction Engineering & Catalysis 2024: BCREC Volume 19 Issue 4 Year 2024 (December 2024)
Publisher : Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.9767/bcrec.20234

Abstract

Native lipase is still promising as an industrial biocatalyst. This study aimed to investigate the production of native local lipase using solid state fermentation (SSF) methods, immobilization the lipase by Ca-alginate entrapment, and characterization based on substrate preferences. To obtain high lipase production using SSF methods, we optimized the type of agro-wastes substrates, fermentation time, oil induction percentage and volume of preculture percentage. The optimal condition for lipase production via solid-state fermentation involved a 7% (v/v) preculture of Pseudomonas aeruginosa SB-37, utilizing palm kernel meal as the substrate, supplemented with 6% (v/w) oil induction (soybean oil:tween 80 = 75:25) at 50 °C for 24 h. This gave a lypolitic activity value of 2 U/gds (gram dry weight substrates). Since the protein profile of extracellular lipase has a few protein bands, we perform direct immobilization on crude protein supernatant. Immobilization by Ca-alginate entrapment results in loading capacity and recovery activity values of 86.84% and 148%, respectively. The immobilized lipase retained 92% activity until four batch repetition and keep 40% activity at tenth batch. The highest hydrolytic activity of immobilized lipase was 0.9 U/g gel on the pNP_8 substrates. While the highest transesterification activity was observed with acetonitrile solvent and substrates of pNP_8 and isopropanol with the activity value at 0.6 U/g gel. This present study emphasized the feasibility of producing lipase as a biocatalysts using economical agro-industrial wastes and efficient immobilization using entrapment method. Copyright © 2024 by Authors, Published by BCREC Publishing Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).
Co-Authors Abraham Tiandno Witjaksono Agus Purwanto Aisyah Az-Zahro Amalia, Berlyana Anisa Wuri Kartika Annisa, Nidya Anshori, M. Wildan Arief Muttaqiin Arifin, Achmad Saiful Asep Purwo Yudi Utomo Astuti, Nisa Sindi Az-Zahro, Aisyah Az-Zahro, Fatimah Dewi, Alfina Kusuma Dikhi Firmansyah Dwi Ariani, Sri Retno Dwi Saryanti Eka Nurul Qomaliyah Elvira Hermawati Erti Hamim Erti Hamimi Evi Nur Mala Sari Fahria, Mila Fardhani, Indra Fatimah Az-Zahro Fatmawati, Fenti Fitroh Rafli Firdausi Habiddin Hanandya Rahma Dinda Destriani Hanifiyah, Fitroh Haryati, Titin Hendra Helmanto Husnayaini, Nida Ilmiatunnisa, Rahmatin Ilmiatunnisa’, Rahmatin Isnanik Juni Fitriyah Khalifatulloh Fiel'ardh Kusumarini, Shelly Lia Dewi Juliawaty Lina Sinatra Wijaya Maghfiroh Maghfiroh Mahardika Agus Wijayanti Mohammad Iqbal Mabruri, Mohammad Iqbal Muhammad Fajar Marsuki Muhardjito Muhardjito Mulyani, Sri Munzil Murty, Daru Anggara Natasya Intania Pertiwi Nida Rufaida Nina Chairani Fatimah Nisa Sindi Astuti Norman Yoshi Haryono Novida Pratiwi Pertiwi, Natasya Intania Prabowo, Himawan Ganjar Praptawati, Atika Purnomo Putri Dzakiyyatul Khotimah Putri, Viola Syarwendah Ramadhan, Miftahul Akbar Rini Anggi Arista Riyanto, Obaja Eden Santosa Rizky Antika Rosita Assyifa Purba Rossi Galih Kesuma Said Hasan Salsabila, Nanda Fitria Santosa, Khalis Nadhira Saryanti, Dwi Setiawan, A. M. Shinta Permata Sari Sugiyarta Stanislaus, Sugiyarta Supargiyono - Syach, Muhammad Zahran Edlian Tri Ratnaningsih Tunazjah, Savina Ulfi Akhyatussyifa Vianni Nifattien Vrisna Putri Wicaksono, Azizul Widadi, Zahir widya astuti Wuri Wuryandani Yana Maolana Syah Yayuk Mulyati Zulfa Fahmy