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Viability of BHK-21 Fibroblast Cells Resulting from Synthesis of Collagen Powder (Thunnus albacares) with Variation of NaCl Concentration Zefanya, Kartika; Rahmitasari, Fitria; Widaningsih, Widaningsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.94-104

Abstract

Background: Collagen plays a crucial role in post-extraction wound healing. Thunnus albacares skin, a collagen source synthesized through the papain soluble collagen (PaSC) method with varying NaCl concentrations, is assessed for non-toxicity using the MTT assay on BHK-21 fibroblast cell cultures. This research is to determine cell viability resulting from synthesizing collagen powder from the skin of yellowfin tuna (Thunnus albacares) with varying NaCl concentrations using the papain soluble collagen (PaSC) method. Method: Collagen powder was synthesized by cleaning Thunnus albacares skin measuring 1x1 cm, soaking it in 0.1 M NaOH, hydrolyzing it with CH3COOH, extracting it with papain enzyme, and then dividing the samples into four groups: without NaCl, NaCl 0.9 M, NaCl 1.3 M, and NaCl 1.7 M. Samples were centrifuged (6000 rpm) and followed by freeze-drying. Cell viability was obtained by conducting a cytotoxicity test using the MTT Assay method on BHK-21 fibroblast cells. Result: The percentage of cell viability in groups K, P1, P2, P3, and P4 were 100%, 10.708%, 113.750%, 107.833%, and 105.958%, respectively. The Kruskall-Wallis test yielded a significance value 0.000, indicating a significant difference (p<0,005). The Mann-Whitney test confirmed significant differences between groups. Conclusion: Collagen powder from Thunnus albacares skin with NaCl concentrations of 0.9 M, 1.3 M, and 1.7 M showed no toxic effects, while the group without NaCl showed toxic effects. Collagen powder with a NaCl concentration of 1.7 M yielded ideal results and showed no toxic effects.
IMPLEMENTASI SISTEM INFORMASI PENGUMUMAN MADRASAH DI MIN 1 KOTA MALANG Hendrawan, Muhammad Afif; Yunianto, Dika Rizky; Muhammad Shulhan Khairy; Sukmana, Septian Enggar; Wijaya, Indra Dharma; Widaningsih, Widaningsih
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 5 No. 1 (2024): Volume 5 No 1 Tahun 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v5i1.19872

Abstract

Pendidikan dasar memainkan peran krusial dalam pembentukan fondasi pendidikan nasional, khususnya untuk anak-anak berumur 7 hingga 15 tahun, sesuai dengan Undang-Undang No. 20 Tahun 2003 Tentang Sistem Pendidikan Nasional di Indonesia. Orang tua, sebagai wali murid, memiliki peran penting dalam mengarahkan perkembangan anak-anak, baik dari segi kognitif, afektif, maupun psikomotorik. Mereka tidak hanya bertanggung jawab atas nilai-nilai moral dan agama, tetapi juga harus mendampingi pendidikan akademik anak-anak mereka. WHO telah mengeluarkan panduan bagi orang tua pada tahun 2020, terutama dalam menghadapi tantangan pandemi, untuk membantu anak-anak mereka dalam proses belajar di rumah. Penelitian ini mengidentifikasi bahwa peran orang tua, khususnya wali murid, memiliki dampak signifikan pada pembelajaran siswa. Selain itu, hubungan yang baik antara sekolah dan wali murid menjadi krusial, dan informasi harus tersampaikan secara efektif. Kasus konkretnya adalah MIN 1 Kota Malang, di mana informasi dan pengumuman disampaikan melalui grup pesan WhatsApp. Namun, masalah muncul karena pesan yang menumpuk dan sulit terbaca oleh wali murid. Untuk mengatasi masalah ini, penulis merancang sebuah sistem informasi pengumuman madrasah. Sistem ini diharapkan akan membantu MIN 1 Kota Malang dalam mengelola penyampaian informasi dan pengumuman sekolah secara lebih efisien kepada wali murid, memperkuat keterlibatan orang tua dalam pendidikan anak-anak, dan memberikan kontribusi positif pada proses pendidikan dasar.
Efektivitas Pelatihan Pegawai Dalam Meningkatkan Kinerja Di Kantor Dinas Pariwisata Dan Kebudayaan Kabupaten Garut Hadita, Akmala; Pundenswari, Pupung; Ulumudin, Aceng; Widaningsih, Widaningsih
Jurnal Publik Vol. 18 No. 01 (2024): Jurnal Publik: Jurnal Ilmiah Bidang Ilmu Administrasi Negara
Publisher : Program Pasca Sarjana Universitas Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52434/jp.v18i01.364

Abstract

Penelitian ini mengkaji efektivitas program bimbingan sejawat di Dinas Pariwisata dan Kebudayaan Kabupaten Garut. Tujuan penelitian ini untuk mengetahui seberapa efektif pelatihan guru dalam meningkatkan produktivitas pegawai di Dinas Pariwisata dan Kebudayaan Kabupaten Garut. Penelitian ini menggunakan metodologi deskriptif kualitatif dengan menggunakan teknik narasumber gambar lilin, observasi di Dinas Pariwisata dan Kebudayaan Kabupaten Garut, serta studi dokumentasi melalui buku, jurnal, artikel ilmiah, dan sumber lainnya. Pelatihan yang dilaksanakan di Dinas Pariwisata dan Kebudayaan Kantor Garut terdiri dari tiga jenis pelatihan yaitu struktural, keuangan, dan kearsipan. Pelatihan yang dilakukan oleh para instruktur di Dinas Pariwisata dan Kebudayaan Kabupaten Garut Meskipun sebagian besar pelatihan tersebut efektif, masih ada beberapa permasalahan dalam cara pembayaran upah kepada para pekerja. Programa pegawaian sangat efektif karena dapat meningkatkan pemahaman pegawai tentang bagaimana mengatur waktu mereka secara efektif.
Development of Performance Management in Refrigerated Transportation Company PT WET Logistics Indonesia Based on the Balanced Scorecard (BSC) Widaningsih, Widaningsih; Dikky Indrawan, Raden; Nurhayati, Popong
Asian Journal of Social and Humanities Vol. 4 No. 4 (2026): Asian Journal of Social and Humanities
Publisher : Pelopor Publikasi Akademika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59888/ajosh.v4i4.668

Abstract

PT WET Logistics Indonesia is a company engaged in the refrigerated truck rental sector. To improve service quality, PT WET Logistics Indonesia has implemented integrated Industry 4.0 technology through the Transportation Management System (TMS), Global Positioning System (GPS), and data thermologger (DTL) across its fleet, positively impacting the company's revenue. Nonetheless, management realizes that developing a long-term strategy cannot rely solely on financial performance indicators and customer satisfaction. This dependence risks delays in responding to changes in the business environment and lost strategic opportunities. Therefore, this research aims to design the stages of strategy formulation by translating the company's vision, mission, and strategy into four Balanced Scorecard (BSC) perspectives and developing a strategic map based on cause-and-effect relationships. The research method involves performance management design aligned with the company's vision and strategy, supported by Focus Group Discussions (FGD) and interviews with stakeholders. The results show that the strategy formulation stage yields an elaboration of the vision, mission, and strategy into four BSC perspectives. The strategic planning stage produces a strategic map, 13 strategic goals, 15 critical success factors (CSF), and 17 key performance indicators (KPIs) with priority targets and weights determined using the Saaty scale (1–9). Furthermore, 39 strategic initiatives were formulated and are monitored through a BSC-based performance management dashboard with a traffic light control system.  
Kepuasan Sebagai Variabel Mediasi Pada Penerapan Komunikasi Terapeutik Perawat terhadap Minat Berkunjung Ulang Prasetyo, Ferdhina Annisa; Widaningsih, Widaningsih
Journal of Hospital Management Vol 4, No 01 (2021): Journal of Hospital Management
Publisher : Lembaga Penerbitan Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47007/.v4i01.4457

Abstract

The background of this research was the number of the patient revisit intention at Tiara Hospital in 2017 had not reached 90%, it only ranged from 78.3% to 83.3%, the rate of patient satisfaction at Tiara Hospital had not reached 95% and nurse communication was a major cause of patient dissatisfaction. The study aimed to analyze the influence of the therapeutic communication application in nursing into patient satisfaction and revisit intention at Tiara Hospital. This study was Causal Explanatory Research with 190 respondents as inpatient at Tiara Hospital Tangerang. The samples were taken using proportionate stratified random sampling technique. The variables of this research were the Therapeutic Communication Application in Nursing as an independent variable, Revisit Intention as the dependent variable, Patient Satisfaction as an intervening variable. The instrument of the research study was a questionnaire with a scale of Likert 5 levels. The data were analyzed using Structural Equation Model (SEM). The results showed that the Therapeutic Communication Application in Nursing had a positive effect on patient satisfaction and Revisit Intention simultaneously, Therapeutic Communication Application in Nursing had a positive effect on patient satisfaction, Patient satisfaction had a positive effect on Revisit Intention. The Therapeutic Communication Application in Nursing positively affected Revisit Intention. Patient Satisfaction was an intervening variable of the Therapeutic Communication Application in Nursing into the Revisit Intention. The findings of this study support the theory of Zeithaml and Bitner (2006) that the higher the level of patient satisfaction, the higher the patient's revisit intenton. The implication of this research was that Tiara Hospital management needs to improve the application of therapeutic communication accompanied by monitoring and evaluation and reduce the workload of nurses in order to increase patient satisfaction and revisit intention.Keywords: Therapeutic Communication, Patient Satisfaction, Revisit Intention
Promoting an Adaptive Educational Planning Model In the Age of Sustainable Development Goals Hartini, Nani; Widaningsih, Widaningsih; Garnasih, Wulan; Prihatin, Eka; Kunmei, Li
Jurnal Ilmu Pendidikan Vol 31, No 2 (2025): December
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um048v31i2p%p

Abstract

This study aims to presents an adaptive education planning model integrating data-driven school mapping, vocational alignment with industry needs, and collaborative governance to enhance educational access and workforce alignment in Indonesia. Using a qualitative approach, we conducted in-depth interviews with 16 informants, including educational policymakers, consultants, and industry representatives. The findings indicate that strategic school placement, guided by demographic data, addresses educational equity by prioritizing underserved areas. Vocational programs aligned with local labor demands equip students with industry-relevant skills, enhancing job absorption and reducing workforce migration. Collaborative governance fosters stakeholder engagement, allowing educational institutions to adapt to socio-economic changes. Practical implications include better resource allocation in underserved regions, improved job market alignment through vocational education, and stronger local economies through talent retention. This model supports Sustainable Development Goal 4 (SDG 4) by creating a responsive, community-centered educational framework that promotes sustainable socio-economic development across Indonesia.
Co-Authors Abdul Chalim Agnita Cevanti, Twi Ahmad Bahauddin Almu’faro Ahmad Bahauddin Almu’faro Akmala Hadita Amalia, Hanny Anisa Anindya Pinasti, Restika Arya Brahmanta, Arya Bayu Hikmat Purwana, Bayu Hikmat Damaiyanti, Dian Widya Dhaifullah, Muhammad Najmi Diana Soesilo, Diana Dika Rizky Yunianto Dikky Indrawan, Raden Dwi Budi Santoso Dwi Maharani, Aulia Elly Nurlia Fadloli Fadloli Fani Pangabdian, Fani Fitria Rahmitasari Garnasih, Wulan Handayani, Budi Hanum, Faizah Hariyanti Hariyanti Harnowo, Setyo Hartini, Nani Hendrawan, Muhammad Afif Iman, Dimas Indra Dharma Wijaya, Indra Dharma Istighna, Luthfiah Nur Kamila, Nisrina Cahya Khoironi, Emy Kunmei, Li Kusumawardhani, Khalyssa Putri Lelyna, Nora Listya Paramita, Annisa Lokman, Asmidar Mahpudin Mahpudin Mawardy, Vivy Mohamad Sinal Muhammad Shulhan Khairy Mulyandika, Gilang Arival Nina Nilawati Noengki Prameswari Novi Agustina Nugroho, Muhammad Bintang Nurdiansah, Erdin Nuryanah, Nuryanah Paramita, Anisa Listya Paramita, Ayulistya Pinasti, Restika Anindya Popong Nurhayati Prasetyo, Ferdhina Annisa Prihatin, Eka Puguh Bayu Prabowo, Puguh Bayu Pupu Pupung Pundenswari, Pupung Puspita, Sinta Rahman, Kurnia Hayati Rahmanawati, Ainin Rizal Maulana, Rizal Rizal, Mohammad Basroni Santoso, Nanda Trio Septian Enggar Sukmana Setiani, Fadila Bela Sihabuddin, Sihabuddin Sinambela, Helen F Sinurat, Henri Prianto Sucahyo, Bambang Suhariningsih Susi Adiawaty Sutarto, Ayulistya Paramita Syamsulina Revianti Ulumudin, Aceng Wawan Wawan Wedarti, Yoifah Rizka Widyasri Prananingrum Yuliyani, Zilha Yuningsih, Miranti Arum Zefanya, Kartika