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All Journal International Journal of Public Health Science (IJPHS) HAYATI Journal of Biosciences Jurnal Veteriner Jurnal Berkala Epidemiologi Indonesian Journal of Tropical and Infectious Disease Biology, Medicine, & Natural Product Chemistry Majalah Obstetri dan Ginekologi Dental Journal (Majalah Kedokteran Gigi) BIOMA Jurnal Respirasi (JR) Majalah Biomorfologi (Biomorphology Journal) Narra J Berkala Penelitian Hayati Journal of Clinical Microbiology and Infectious Diseases Prosiding Seminar Nasional COSMIC Kedokteran Strada Jurnal Ilmiah Kesehatan (SJIK) Folia Medica Indonesiana
Kuntaman Kuntaman
Faculty Of Medicine, Universitas Wijaya Kusuma Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Veterinary

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Journal : Narra J

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Correlation of miR-150, hsa-let-7e, and miR-146a and gene expression of IL-6, IL-8, IP-10, and MIP-1β during dengue virus infection Sri Masyeni; Kuntaman Kuntaman; Aryati Aryati; Muchlis AU Sofro; Usman Hadi; Gondo Mastutik; Windu Purnomo; Agus Santosa; Benediktus Yohan; Erni Juwita Nelwan; R. Tedjo Sasmono
Narra J Vol. 1 No. 1 (2021): April 2021
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narraj.v1i1.31

Abstract

Growing evidence suggests that microRNAs (miRNAs) play a pivotal role in viral infection. The objective of this study was to assess the association between the expression of miR-150, hsa-let-7e, and miR-146a on cytokine expression during dengue infection. Dengue virus (DENV) strain SJN-006, a serotype 2 DENV strain of the Cosmopolitan genotype, isolated in Bali, Indonesia, was used to infect peripheral blood mononuclear cells (PBMCs) isolated from healthy individuals. The relative gene expressions of miR-150, hsa-let-7e, and miR-146a as well as the gene expression of cytokines (IL-6, IL-8, IP-10, and MIP-1β) were determined using quantitative real time - polymerase chain reaction (qRT-PCR) at 6, 12 and 24 hours post infection (hpi). Correlations between the microRNAs and cytokines were analyzed by means of causality tests. Our data suggests that miR-150 and hsa-let-7e were significantly higher in infected-PBMCs after 12 hpi compared to the uninfected-PBMCs (p<0.05). The causality tests demonstrated that miR-150 and hsa-let-7e were negatively correlated with IL-8 expression, meanwhile miR-146a was the contrast. DENV infection was negatively and positively correlated with miR-150 and hsa-let-7e, respectively, after 24 hpi. In conclusion, our data demonstrates the vital role of miR-150, hsa-let-7e, and miR-146a in regulating IL-8 expression with possible different pathways.
Correlation of miR-150, hsa-let-7e, and miR-146a and gene expression of IL-6, IL-8, IP-10, and MIP-1β during dengue virus infection Masyeni, Sri; Kuntaman, Kuntaman; Aryati, Aryati; Sofro, Muchlis AU; Hadi, Usman; Mastutik, Gondo; Purnomo, Windu; Santosa, Agus; Yohan, Benediktus; Nelwan, Erni Juwita; Sasmono, R. Tedjo
Narra J Vol. 1 No. 1 (2021): April 2021
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narraj.v1i1.31

Abstract

Growing evidence suggests that microRNAs (miRNAs) play a pivotal role in viral infection. The objective of this study was to assess the association between the expression of miR-150, hsa-let-7e, and miR-146a on cytokine expression during dengue infection. Dengue virus (DENV) strain SJN-006, a serotype 2 DENV strain of the Cosmopolitan genotype, isolated in Bali, Indonesia, was used to infect peripheral blood mononuclear cells (PBMCs) isolated from healthy individuals. The relative gene expressions of miR-150, hsa-let-7e, and miR-146a as well as the gene expression of cytokines (IL-6, IL-8, IP-10, and MIP-1β) were determined using quantitative real time - polymerase chain reaction (qRT-PCR) at 6, 12 and 24 hours post infection (hpi). Correlations between the microRNAs and cytokines were analyzed by means of causality tests. Our data suggests that miR-150 and hsa-let-7e were significantly higher in infected-PBMCs after 12 hpi compared to the uninfected-PBMCs (p<0.05). The causality tests demonstrated that miR-150 and hsa-let-7e were negatively correlated with IL-8 expression, meanwhile miR-146a was the contrast. DENV infection was negatively and positively correlated with miR-150 and hsa-let-7e, respectively, after 24 hpi. In conclusion, our data demonstrates the vital role of miR-150, hsa-let-7e, and miR-146a in regulating IL-8 expression with possible different pathways.
The role of mediator suppressor of cytokine signaling (SOCS), toll-like receptor 3 (TLR-3) and nuclear factor kappa B (NFκB) on cytokine production during dengue virus infection Masyeni, Sri; Kuntaman, Kuntaman; Aryati , Aryati; Sofro, Muchlis AU.; Hadi, Usman; Mastutik, Gondo; Purnomo, Windu; Santosa, Agus; Iqhrammullah, Muhammad; Yohan, Benediktus; Nelwan, Erni J.; Sasmono, R. Tedjo
Narra J Vol. 3 No. 2 (2023): August 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v3i2.167

Abstract

Inability to understand the pathogenesis of severe dengue, in particular the control mechanism of immune responses, has led to high mortality rate for patients with dengue shock syndrome (DSS). The aim of this study was to determine the control mechanism of cytokine production by mediator suppressor of cytokine signaling (SOCS), toll-like receptor 3 (TLR-3) and nuclear factor kappa B (NFκB) during DENV infection. Peripheral blood mononuclear blood cells (PBMC), isolated from healthy individuals, were infected with dengue virus (DENV)-2 strain SJN-006 Cosmopolitan genotype (isolated from Bali, Indonesia). The relative gene expression of SOCS-3, TLR-3, NFκB, and the cytokine genes (interleukin (IL)-6, IL-8, interferon inducible protein 10 (IP-10), and macrophage inflammatory protein-1 beta (MIP-1β)) were measured using qRT-PCR at 6, 12 and 24 hours post infection (hpi). Student t-test and Mann-Whitney test were used to compare the gene expressions while causal correlations were analyzed using regression test and path analyses. DENV-2 infection increased the gene expression of SOCS-3, TLR-3, and NFκB after 12 and 24 hpi. The expression of IL-6, IL-8, IP-10, and MIP-1β genes was increased and peaked at different times post-infection. NFκB and SOCS-3 genes likely have role in the upregulation of IL-8 and IL-6 gene expression, respectively. MIP-1β gene expression was significantly induced by both NFκB and SOCS-3. In conclusion, our study suggested that SOCS-3, TLR-3, and NFκB are important in regulating the production of IL-6, IL-8, IP-10, MIP-1β during early phase of DENV-2 infection. This enriches our understanding on pathogenesis pathway of DENV-associated cytokine storm.
Co-Authors A. M. Widya A.A.W., I Wayan Putra Abdul Khairul Rizki Purba Mustofa Indwiani Astuti AFAF BAKTIR Agung Dewi Sekar Agus Santosa Agus Santosa akhmad sudibya, akhmad Alimsardjono, Lindawati Aquaresta, Febriana Ardani, I Gusti Wahju Ardhiya Puspita Ardi Eko Marsanto Arina Setyaningtyas Aryati , Aryati Aryati Aryati Aryati Aryati Bahraini, Fahrisah Nurfadeliah Benediktus Yohan Binti Mu’arofah Bramantono Bramantono, Bramantono Budi Prasetyo Budi Utomo Budiono Budiono Budiono Budiono Deby Kusumaningrum Dewi, Lisa Dharmawati, Ira Dian Neni Naelasari Dian Rachmawati Diani Dwi Indrasari Didik Hasmono Dwiyanti Puspitasari, Dwiyanti Eddy Bagus Wasito Eko B. Koendhori Eko Budi Khoendori Eko Budi Koendhori, Eko Budi Erni J. Nelwan, Erni J. Erni Juwita Nelwan, Erni Juwita Fourier Dzar Eljabbar Latief Gondo Mastutik Happy, Terza Aflika Hardiono Hardiono Hardiono Hardiono, Hardiono Hari Basuki Notobroto Hari Paraton Hari Paraton Hidayah, Ana Nurlaili Ida Bagus Narmada Iqhrammullah, Muhammad Ira Widjiastuti Irwanto Irwanto Ishak Samuel Wuwuti Kartuti Debora, Kartuti Kusbaryanto L. Alimsardjono Latief Mooduto, Latief Linda Dewanti Listyawati, Agusniar Furkani Ma'rifah, Nurul Maftuchah Maftuchah Margyaningsih, Nur Ita Maria Inge Lusida Maria Inge Lusida Marinda Dwi Puspitarini Mariyatul Qiptiyah, Mariyatul Marsheila Harvy Mustikaningtyas Masfufatun Masfufatun Masyeni, Sri Muchlis AU Sofro N. D. Kurniati Naritha Noor Cholies Zaini Nur Dianawati Nurul Ma'rifah P. N. Endraputra Parawidnyaningsih, Putu Ayu Aryanda Pristiawan Navy Endraputra Purnomo, Windu Puspitarini, Marinda Dwi Putu Arya Suryanditha R. Tedjo Sasmono Radita Yuniar Arizandy Rakhmatul Binti Sulistya Rasita, Yoeke Dewi Regita Cahyani, Nafansya Rini Devijanti Ridwan Risnanda, Kadek Rio Rosantia Sarassari Rosantia Sarassari Rosantia Sarassari Rosantia Sarassari S. R. S. Oktaviani S. Soedarsono Sacharissa Zerlina Tsarwah Thirafi Sarassari, Rosantia Sasmono, R. Tedjo Semedi, Bambang Pujo Setyarini, Wahyu Sholeh, Mohammad Akbaruddin Silvia Sutandhio Soekoyo, Agusta Reny Sofro, Muchlis AU Sofro, Muchlis AU. Sri Masyeni, Dewa Ayu Putri Sulistiawati Sulistiawati Sulistya, Rakhmatul Binti Syaiful, Irbasmantini Tamara Yuanita Terza Aflika Happy Thayyib, Muqoddar Triffit Imasari UNTUNG MURDIYATMO Usman Hadi Wahyu Setyarini Wahyu Setyorini Wahyu, Agung Dwi Wahyuni Dyah Parmasari, Wahyuni Dyah Windu Purnomo Wiwiek Tyasningsih WJ. Pudjirahardjo Yasmeen Lashari Yoeke Dewi Rasita Yohan, Benediktus