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All Journal Jurnal Natur Indonesia Jurnal Ilmu Kedokteran (Journal of Medical Science) Jurnal Kedokteran Brawijaya Global Medical and Health Communication The Indonesian Biomedical Journal Jurnal Ilmiah Pertanian Jurnal Pengabdian UntukMu NegeRI Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Gizi Klinik Indonesia Jurnal Kedokteran dan Kesehatan Manuju : Malahayati Nursing Journal PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Ners Acta Chimica Asiana Unri Conference Series: Community Engagement Jurnal Katalisator Biomedika Media Penelitian dan Pengembangan Kesehatan Biodiversitas Journal of Biological Diversity
Ismawati ismawati
Department Biochemistry, Faculty Of Medicine, Universitas Riau, Jl. Diponegoro No 1, Pekanbaru
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences

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Vitamin D enhances migration but decreases gene expression of vascular endothelial growth factor and tumor necrosis factor-α in Wharton’s jelly mesenchymal stem cells Vitriyanna Mutiara Yuhendri; Nurfi Pratiwi; Ariza Julia Paulina; Ismawati Ismawati; Nicko Pisceski Kusika Saputra; Arfianti Arfianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in Biomarkers, Therapeutics, and Probiotics: Recent Updates in Medical L
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6698

Abstract

Mesenchymal stem cells (MSCs) have gained significant interest for their potential application in repairing damaged tissues through immunomodulatory functions. Vitamin D has been shown to regulate both the innate and adaptive immune systems. Active forms of Vitamin D, such as 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) are known to influence the action of mesenchymal stem cells (MSCs) in several mechanisms, such as stimulating their proliferation and osteogenic differentiation, as well as tissue regeneration. This study aimed to investigate the effects of vitamin D supplementation on the biological properties and expression of cytokines and growth factor genes in MSCs isolated from the human umbilical cord (UC). MSCs were isolated from Wharton’s jelly (WJ-MSC) of UC, cultured, and supplemented with various concentrations of vitamin D3. The Cell Counting Kit-8 (CCK-8) assay was used to measure cell viability, and a scratch wound healing assay was conducted to evaluate the migration capacity of MSCs. The mRNA expression levels of vascular endothelial growth factor (VEGF), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 were quantified using reverse transcription-polymerase chain reaction (RT-PCR). This study showed that supplementation with 50 nM of vitamin D3 for 48 h significantly increased the viability and migratory capacity of MSCs. Furthermore, vitamin D supplementation significantly decreased the mRNA levels of TNF-α and VEGF but did not affect IL-6 gene expression compared to the control group. These findings suggest that vitamin D supplementation can enhance the biological characteristics and modulate the expression of key immunomodulatory factors in MSCs, potentially improving the effectiveness of MSC-based therapies.
Revitalisasi Makanan Fermentasi Tradisional Kuantan Singingi melalui Pelatihan Berbasis IPTEKS Ismawati Ismawati; Saryono Saryono; Mukhyarjon Mukhyarjon; Enikarmila Asni; Silvera Devi; Suci Anggraini; Nabella Suraya; Lunari Kesya; Dinda Salsabila; Cantika Natswa Deanra
Unri Conference Series: Community Engagement Vol 7 (2025): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.7.531-536

Abstract

Malnutrition, especially among children under five, remains a significant public health concern in Indonesia, including Riau Province. One potential effort to address this issue is the utilization of traditional fermented foods from Kuantan Singingi Regency, such as pekasam, sarobuong, puduong, and cangkuok semaung. These foods are rich in nutrients, including protein, carbohydrates, amino acids, minerals, and probiotics beneficial to health; however, they are on the verge of extinction.This community service program aimed to revitalize traditional Kuansing fermented foods through a science and technology-based training approach. The program implementation involved video demonstrations of traditional food preparation, distribution of pocket books, and interactive discussions with health cadres, as well as pregnant and breastfeeding mothers, and mothers with toddlers.The results showed high enthusiasm among participants, demonstrated by their active involvement in discussions and question-and-answer sessions. The training not only improved participants’ knowledge but also fostered awareness and a sense of ownership of local food, while simultaneously opening opportunities for small-scale household businesses.
Isolation and identification of lactic acid bacteria from traditional food sarobuong of Kuantan Singingi District, Riau, Indonesia SARYONO SARYONO; ISMAWATI ISMAWATI; NOVA WAHYU PRATIWI; SILVERA DEVI; MERRY YOHANA SIPAYUNG; NABELLA SURAYA
Biodiversitas Journal of Biological Diversity Vol. 24 No. 4 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240432

Abstract

Abstract. Saryono, Ismawati, Pratiwi NW, Devi S, Sipayung MY, Suraya N. 2023. Isolation and identification of lactic acid bacteria from traditional food sarobuong of Kuantan Singingi District, Riau, Indonesia. Biodiversitas 24: 2201-2206. Lactic acid bacteria (LAB) are commonly used as probiotic agents in fermented processed foods like sarobuong. Sarobuong is a traditional food from Kuantan Singingi District made by fermenting bamboo shoots. In this study, the isolation and purification of LAB from sarobuang were done using MRS agar media with the streak quadrant method: macroscopic and microscopic observations and biochemical and physiological tests to identify isolates. The antibacterial ability of LAB isolates was used against pathogenic bacteria Escherichia coli and Staphylococcus aureus. The results showed that 21 isolates were gram-positive bacteria in the form of bacilli and coccus, while carbohydrate degradation was homofermentative. The results of the antibacterial test of 21 isolates indicated by LAB against E. coli and S. aureus bacteria showed a clear zone ranging from 0.1 to 0.6. The highest antibacterial activity against pathogenic bacteria E. coli was observed in isolate RB1, while for pathogenic bacteria S. aureus, it was in isolate R15. The results obtained from several isolates that have been tested showed that LAB isolated from sarobuong had relatively higher activity than the control and was identified as Lactobacillus and Streptococcus.
Efek Protektif Suplementasi Ekstrak Etanol Daun Seri (Muntingia calabura) Terhadap Perubahan Histologis Pankreas Tikus Yang Diinduksi Diet Tinggi Lemak Mukhyarjon Mukhyarjon; Irwan Irwan; Ismawati Ismawati
Jurnal Ilmu Kedokteran Vol 20, No 1 (2026): Jurnal Ilmu Kedokteran
Publisher : Fakultas Kedokteran Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26891/JIK.v20i1.2026.59-64

Abstract

Diabetes mellitus is a major global health problem. Lipotoxicity is a key cause of insulin resistance that ultimately leads to pancreatic damage in type 2 diabetes mellitus. One plant with potential to prevent pancreatic damage is the Seri plant (Muntingia calabura). This study aimed to analyze the effect of ethanolic extract of Muntingia calabura leaves on pancreatic damage in rats induced by a high-fat diet (HFD). This was an experimental study with a post-test-only design. A total of 28 male Wistar rats weighing 120–200 grams were divided into four groups: K1 received standard feed (20 g/rat/day) and water ad libitum; K2 received a 50% high-fat diet (20 g/rat/day); K3 received the same HFD plus Muntingia calabura leaf extract (100 mg/kg/day); and K4 received HFD plus pioglitazone (6 mg/kg/day). Treatments were administered for 8 weeks, followed by termination for pancreatic histological examination. The variables studied were supplementation of Muntingia calabura ethanolic extract and the degree of pancreatic insulitis. Histological preparations were stained with hematoxylin-eosin, and insulitis was scored. Data were analyzed using IBM SPSS with Kruskal–Wallis and Mann-witney tests. Results showed no insulitis in K1, while mild insulitis was observed in K2, K3, and K4. A significant difference in histological scores was found between K1 and K2 (p = 0.03), whereas no significant differences were observed among other groups. In conclusion, 8 weeks of HFD induced mild insulitis in rats, while Muntingia calabura leaf extract and pioglitazone did not demonstrate protective effects against insulitis.
EFFECT OF INULIN EXTRACT FROM DAHLIA TUBER (dahlia variabilis) ON CORONARY ARTERY HISTOPATHOLOGY IN RATS WITH TYPE 2 DIABETES MELLITUS (DM) Zikrilla mayuli Hopi; Ismawati Ismawati; Saryono Saryono; Ilhami Romus; Veni Dayu Putri; Sri Yanti; Fitri Dyna
Biomedika Vol 17, No 2 (2025): Biomedika August 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v17i2.3734

Abstract

Atherosclerosis in diabetes mellitus (DM) patients is prevented by using inulin to increase glucose metabolism and reduce cholesterol synthesis. Therefore, this research aimed to analyze dahlia tuber inulin extract to determine the effect on coronary artery histopathology in type 2 DM rats. A total of 20 male Wistar rats were divided into five groups, namely negative control without treatment, DM, DM + 0.5 mg/gBW/day inulin extract, DM + 1 mg/gBW/day inulin extract, and DM + 1.5 mg/gBW/day inulin extract. Furthermore, DM induction was carried out by administering 60 mg/kgBB streptozotocin and 120 mg/kgBB nicotinamide. The results of coronary artery histopathology readings showed that the highest and lowest scores of atherosclerosis were in Groups II (0,91±0,17) and V (0,08±0,1), respectively. Data analysis using Kruskal Wallis test reported significant results (p<0.05), while Mann-Whitney test showed differences between control and DM, DM, and inulin groups (III, IV, V). In this context, the administration of dahlia tuber inulin extract to rats induced by Type 2 DM prevented atherosclerosis in histopathological image of coronary artery.
EFFECT OF DAHLIA TUBER INULIN EXTRACT ON THE THICKNESS OF THE THORACIC AORTIC TUNICA INTIMA-MEDIA IN TYPE 2 DIABETIC RATS MODEL Cantika Natswa Deanra; Ismawati Ismawati; Saryono Saryono; Desby Juananda; Suci Anggraini; Timotius Agung S; Veni Dayu Putri; Sri Yanti; Fitri Dyna
Biomedika Vol 18, No 1 (2026): Biomedika February 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v18i1.4218

Abstract

Inulin is a natural polysaccharide that improves glucose metabolism and reduces cholesterol levels through the formation of short-chain fatty acids, which may contribute to preventing atherosclerosis in type 2 diabetes mellitus (T2DM). Therefore, this study aimed to evaluate the effect of dahlia tuber inulin extract on the thickness of the thoracic aortic tunica intima-media (TIM) in T2DM-induced rats. A total of 20 male Wistar rats were divided into five groups: control, T2DM, T2DM + 0.5 mg/gBW/day inulin extract, T2DM + 1.0 mg/gBW/day inulin extract, and T2DM + 1.5 mg/gBW/day inulin extract. T2DM induction was performed using 60 mg/kgBW streptozotocin and 120 mg/kgBW nicotinamide. Measurement of TIM thickness was conducted using a Leica light microscope with hematoxylin-eosin staining. The results showed no statistically significant differences among the groups (p = 0.051), although a decreasing trend in TIM thickness was observed at the 1.5 mg/gBW/day dose. In this context, dahlia tuber inulin extract demonstrated a tendency to reduce TIM thickness in T2DM-induced rats.
Hubungan Kadar Prostaglandin E2 dan Interleukin-6 dengan Derajat Nyeri dan Stadium Endometriosis Muhammada Ardaris Alhudri; Imelda EB Hutagaol; Ismawati Ismawati
Malahayati Nursing Journal Vol 8, No 10 (2026): Volume 8 Nomor 10 (2026)
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mnj.v8i10.26265

Abstract

ABSTRACT Endometriosis is a chronic gynecological disease characterized by pelvic pain and persistent inflammatory processes. Prostaglandin E2 (PGE2) and Interleukin-6 (IL-6) are inflammatory mediators involved in peripheral and central pain sensitization as well as disease progression. However, their association with clinical indicators such as pain severity and disease stage remains inconsistent. This study aimed to determine the relationship between PGE2 and IL-6 levels in peritoneal lavage fluid with pain severity and endometriosis stage. This analytical observational study with a cross-sectional design included 25 endometriosis patients scheduled for laparoscopic surgery at Arifin Achmad General Hospital, Pekanbaru. Peritoneal lavage fluid samples were collected intraoperatively and analyzed using ELISA. Pain severity was assessed using the Visual Analog Scale (VAS), while endometriosis staging was classified according to ASRM criteria. Statistical analysis was conducted using the Kruskal–Wallis test and Spearman correlation. Mean PGE2 levels increased progressively with pain severity (317.38 pg/mL in mild pain; 547.97 pg/mL in moderate pain; 766.55 pg/mL in severe pain), showing a strong positive correlation (r = 0.764; p = 0.001). IL-6 levels showed no significant association with pain severity (r = –0.287; p = 0.296). Regarding endometriosis staging, IL-6 demonstrated a very strong positive correlation (r = 0.947; p 0.001), whereas PGE2 showed a weak and non-significant negative correlation (p = 0.386). PGE2 levels are significantly associated with pain severity, while IL-6 levels are significantly associated with endometriosis stage. These findings highlight the potential of PGE2 and IL-6 as complementary biomarkers for assessing pain and disease progression in endometriosis. Keywords: Endometriosis, Prostaglandin E2, Interleukin-6, Pain Severity, Disease Stage.  ABSTRAK Endometriosis merupakan penyakit ginekologis kronis yang ditandai oleh nyeri panggul dan proses inflamasi berkelanjutan. Prostaglandin E2 (PGE2) dan Interleukin-6 (IL-6) merupakan mediator inflamasi yang berperan dalam proses sensitisasi nyeri perifer dan sentral serta progresivitas penyakit. Namun, hubungan keduanya dengan indikator klinis seperti derajat nyeri dan stadium endometriosis masih belum konsisten. Penelitian ini bertujuan untuk mengetahui hubungan kadar PGE2 dan IL-6 dalam cairan bilasan peritoneum dengan derajat nyeri dan stadium endometriosis. Penelitian analitik observasional dengan desain cross sectional dilakukan pada 25 pasien endometriosis yang direncanakan tindakan laparaskopi di RSUD Arifin Achmad, Pekanbaru. Sampel cairan bilasan peritoneum diambil selama tindakan laparoskopi dan dianalisis menggunakan metode ELISA. Derajat nyeri dinilai dengan Visual Analog Scale (VAS), sementara stadium endometriosis diklasifikasikan berdasarkan kriteria ASRM. Analisis statistik menggunakan uji Kruskal–Wallis dan korelasi Spearman. Rerata kadar PGE2 meningkat seiring peningkatan derajat nyeri (317,38 pg/mL pada nyeri ringan; 547,97 pg/mL pada nyeri sedang; 766,55 pg/mL pada nyeri berat), dengan korelasi positif kuat (r = 0,764; p = 0,001). Kadar IL-6 tidak menunjukkan hubungan bermakna dengan derajat nyeri (r = –0,287; p = 0,296). Pada stadium endometriosis, IL-6 menunjukkan korelasi positif sangat kuat (r = 0,947; p 0,001), sedangkan PGE2 menunjukkan korelasi negatif lemah yang tidak signifikan (p = 0,386). Kadar PGE2 memiliki hubungan bermakna dengan derajat nyeri, sedangkan kadar IL-6 berhubungan bermakna dengan stadium endometriosis. Temuan ini menunjukkan potensi PGE2 dan IL-6 sebagai biomarker komplementer dalam evaluasi nyeri dan progresivitas endometriosis. Kata Kunci: Endometriosis, Prostaglandin E2, Interleukin-6, Derajat Nyeri, Stadium Endometriosis.
Perbandingan Ekspresi Interleukin-6 dan Early Healing Score Sebelum dan Sesudah Pemberian Platelet Rich Plasma pada Pasien Servisitis Kronis Rudolf Fernando Turnip; Sri Wahyu Maryuni; Ismawati Ismawati
Malahayati Nursing Journal Vol 8, No 10 (2026): Volume 8 Nomor 10 (2026)
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mnj.v8i10.26186

Abstract

ABSTRACT Chronic cervicitis is a persistent inflammatory condition of the cervical tissue caused by repeated microbial exposure, trauma, or unresolved acute infection. Conventional antibiotic therapy is often insufficient to promote optimal tissue regeneration. Platelet-Rich Plasma (PRP) has emerged as a regenerative adjuvant therapy with promising anti-inflammatory and healing properties. This study aimed to compare Interleukin-6 (IL-6) expression and Early Healing Score (EHS) before and after PRP injection in patients with chronic cervicitis. This was a clinical trial conducted at the Division of Urogynecology and Reconstructive Aesthetics, Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Riau / RSUD Arifin Achmad Pekanbaru from April to September 2025. A total of 38 patients diagnosed with chronic cervicitis were randomly allocated into two groups: antibiotics + PRP (n=20) and antibiotics only (n=18). IL-6 expression was measured using ELISA before and 14 days after intervention. EHS was assessed 7 days post-therapy. The antibiotic + PRP group showed significantly higher total EHS compared to the antibiotic-only group (7.850 ± 0.812 vs 6.555 ± 0.855; p 0.001), with significant improvement in re-epithelialization (p=0.009) and haemostasis (p=0.047) components. IL-6 expression at 14-day follow-up was significantly lower in the combination group (0.0088 ± 0.0019 pg/mL vs 0.0219 ± 0.0051 pg/mL; p=0.027). No adverse events were recorded in either group. PRP injection as an adjuvant therapy significantly improves early wound healing and reduces IL-6 expression in chronic cervicitis patients, with a favorable safety profile. Keywords: Platelet Rich Plasma, Chronic Cervicitis, Interleukin-6, Early Healing Score, Wound Healing.  ABSTRAK Servisitis kronis merupakan kondisi inflamasi persisten pada jaringan serviks yang sering disebabkan oleh paparan mikroba berulang, trauma, atau resolusi infeksi akut yang tidak sempurna. Terapi antibiotik konvensional seringkali tidak memadai untuk mendorong regenerasi jaringan secara optimal. Platelet-Rich Plasma (PRP) telah muncul sebagai terapi adjuvan regeneratif dengan sifat anti-inflamasi dan penyembuhan yang menjanjikan. Penelitian ini bertujuan membandingkan ekspresi Interleukin-6 (IL-6) dan Early Healing Score (EHS) sebelum dan sesudah pemberian injeksi PRP pada pasien servisitis kronis. Penelitian ini merupakan uji klinis yang dilaksanakan di Divisi Uroginekologi Rekonstruksi dan Estetik Departemen Obstetri dan Ginekologi FK Universitas Riau / RSUD Arifin Achmad Pekanbaru dari bulan April hingga September 2025. Sebanyak 38 pasien dengan diagnosis servisitis kronis diacak menjadi dua kelompok: antibiotik + PRP (n=20) dan antibiotik saja (n=18). Ekspresi IL-6 diukur dengan metode ELISA sebelum dan 14 hari setelah intervensi. EHS dinilai 7 hari pasca-terapi. Kelompok antibiotik + PRP menunjukkan skor total EHS yang secara signifikan lebih tinggi dibandingkan kelompok antibiotik saja (7,850 ± 0,812 vs 6,555 ± 0,855; p 0,001), dengan perbaikan bermakna pada komponen re-epitelisasi (p=0,009) dan hemostasis (p=0,047). Ekspresi IL-6 pada follow-up 14 hari secara signifikan lebih rendah pada kelompok kombinasi (0,0088 ± 0,0019 pg/mL vs 0,0219 ± 0,0051 pg/mL; p=0,027). Tidak ditemukan kejadian adverse event pada kedua kelompok. Injeksi PRP sebagai terapi adjuvan secara signifikan meningkatkan penyembuhan luka dini dan menurunkan ekspresi IL-6 pada pasien servisitis kronis dengan profil keamanan yang baik. Kata Kunci: Platelet Rich Plasma, Servisitis Kronis, Interleukin-6, Early Healing Score, Penyembuhan Luka.
Co-Authors Adinda Aisyah Afif P Jati Afifah Ramadhani Angel Chen Anggraini, Suci Ardhi, Aulia Arfianti Arfianti Ariqa Rahma Abdullah Ariza Julia Paulina Cantika Natswa Deanra Cantika Natswa Deanra Deanra, Cantika Natswa Desby Juananda Dhea Ayu Kartini Surya Putri Dinda Salsabila Dyna, Fitri Edy Fachrial Effendi, Fallgivando Elfima Aditya Utami Enikarmila Asni Enikarmila Asni Enikarmila Asni Erwin Christianto Esy Maryanti Fitri Dyna Fitri Dyna Ilhami Romus Ilhami Romus Ilhami Romus Ilhami Romus Ilhami Romus, Ilhami Imelda EB Hutagaol Imelda EB Hutagaol Imelda Tresia Pardede Indah Putri Arwandi Irfani Dhia Rafif Irwan Irwan Isnani Kurniyanti Kesya, Lunari Ligat Pribadi Sembiring Lovenia, Nia Lunari Kesya Lusi Yusrenti Luthfianty, Elfiah M Derillovyandra Dwi Anugrah M Yulis Hamidy MERRY YOHANA SIPAYUNG Mifta Zahira Muhammad Ardaris Alhudri Muhammad Fadhillah Arif Muhammada Ardaris Alhudri Mukhyarjon NABELLA SURAYA Nabella Suraya Nabella Suraya Nazla Hafizah Nicko Pisceski Kusika Saputra NOVA WAHYU PRATIWI NOVA WAHYU PRATIWI, NOVA WAHYU Noviardi Noviardi, Noviardi Nurafriza, Widya Nurfi Pratiwi Permatasari, Nopi Putra, Amrullah Syah Qryoga Irsyad Reyen Raissa Maharani Putik Kusuma Rijalun Arridho Rudolf Fernando Turnip Salsabila Nurul Wildani Hasibuan Salsabila, Dinda Saputra, Nicko Pisceski Kusika Saryono Saryono SARYONO SARYONO Saryono Saryono Saryono Saryono Saryono Saryono Saryono Saryono Sasmita, Ellen Rosyelina Sera Faren SILVERA DEVI Silvera Devi Sonia Dinda Paramitha Sri Wahyu Maryuni Sri Yanti Sri Yanti Sri Yanti Suci Anggraini suraya, nabella Timotius Agung S Timotius Agung Soripada Titania T Nugroho Titania Tjandrawati Nugroho Veni Dayu Putri Veni Dayu Putri Veni Dayu Putri Vitriyanna Mutiara Yuhendri Winarto Winarto Zikrilla mayuli Hopi