Claim Missing Document
Check
Articles

Pemanfaatan Ampas Tahu Sebagai Bahan Baku Kecap Melalui Bioproses Rhizopus spp. Umrah Umrah; Nur Asrinawaty; Muhammad Alwi
Biocelebes Vol. 5 No. 2 (2011)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian Pemanfaatan Ampas Tahu Sebagai Bahan Baku Kecap Melalui Bioproses R.hizopus spp. telah dilaksanakan pada bulan April-Juni 2011. Adapun tujuan dan penelitian ini yaitu untuk mengetahui pengaruh lama bioproses Rhizopus spp., terhadap kadar protein kecap ampas tahu dan kesesuaian dengan standar mutu kecap di Indonesia serta pengaruhnya terhadap aroma dan cita rasa kecap ampas tahu. Penelitian dilakukan di laboratorium Bioteknologi dan di Laboratorium Agroteknologi. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap dengan perlakuan lama bioproses Rhizopus spp.: P0 (tanpa fermentasi), P1 (fermentasi satu han). P2 (fermentasi dau han) dan P3 (fermnetasi tiga han). Penentuan kadan protein dilakukan dengan menggunakan metode spektrofotometer. Hasil penelitian menunjukkan bahwa lama bioproses Rhizopus spp. berpengaruh nyata pada kadar protein kecap ampas tahu dengan perlakuan yang terbaik adalah P2 (fermentasi 2 han) sebesar 7,29%. Selama 3 hari proses fermentasi, kadar protein meningkat. Kadar protein tersebut telah sesuai dengan standar mutu kecap nomor satu di Indonesia. Lama bioproses Rhizopus spp. berpengaruh nyata pada aroma kecap ampas tahu tetapi tidak berpengaruh pada cita rasa kecap ampas tahu. Key words: Ampas tahu, kecap , Rhizopus spp., dan kadar protein.
Uji Keefektivan Formula Trichoderma sp. Sediaan Tablet Sebagai Dekompuser Limbah Organik Ampas Sagu Menjadi Biokompos Umrah Umrah; Muhammad Alwi; Muh. Arsal N. Maddo
Biocelebes Vol. 5 No. 2 (2011)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Research entitle of “Bioactive compost from sago waste and chicken manure through bioprocess using Trichoderma sp.” have been conducted during March and July 2011. This research have been done in Biotechnology Laboratory, Science Fakulty of Tadulako University. This aims of this study are to produce bioactive compost from waste of sago pulp and chicken manure through bioprocess Trichoderma sp., also to determine the optimum dose of inoculums of Trichoderma sp.. This study was done experimentally and it was based on a completely randomized design. Result of this study showed that P2 (substrate + 2 tablets inoculum/kg substrate) was the best treatment. Key words: Bioactive compost , sago waste, chicken manure, Trichoderma sp.
Formulasi Media Tumbuh Acetobacter xylinum Dari Bahan Limbah Cair Tempe dan Air Kelapa Untuk Produksi Nata De Soyacoco Muhammad Alwi; Andi Lindhemuthianingrum; Umrah Umrah
Biocelebes Vol. 5 No. 2 (2011)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The impact of tempeh liquid-waste (Limbah Cair Tempe, LCT) as Contaminant has become a serious environmental problem. This study was design as an alternative problem solving related to that issue. Combination of tempeh liquid-waste and coconut water (Air Kelapa, AK) can be utilized as a medium of nata de soyacoco production. This research was aimed to obtain the best medium formulation for Acetobacter xylinum in order to produce the bacterial cellulose. This experiment was arranged in completely randomized design with 6 treatments and 3 replications. The treatments were P0 (LCT 0% + AK 0%), P1 (LCT 0% + AK 100%), P2 (LCT 25% + AK 75%), P3 (LCT 50% + AK 50%), P4 (LCT 75% + AK 25%), and P5 (LCT 100% + AK 0%). Parameters observed in this experiment were the days appear of nata, thickness, fresh weight, rendement and texture of nata which tested organolepticly. The best medium formulation for nata de soyacoco production was P4 (LCT 75% + AK 25%), which resulted 1.04 cm thickness, 139.48 gram fresh weight, 42.27% rendement and 1.7 of texture value. Key words: tempeh liquid water, coconut water, Acetobacter xylinum.
Identifikasi Actinomycetes yang Terdapat Pada Tanah di sekitar Danau Lindu Sulawesi Tengah Muhammad Alwi; Latifah Merdekawaty; Umrah Umrah
Biocelebes Vol. 6 No. 1 (2012)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The research about “Identification of Actynomycetes from soil around of Lindu Lake” from September to Desember 2010 the purpose was receiving, to identificate, and screening of Actinomycetes from soil around of Lindu Lake. The metodology is descriptive it means that all character data of isolat fenotive Actynomicetes were reSp.sentatively to be applied for identificating conventionally by applying the key book of identification. The result of this research was obtained six isolats Actynomycetes resepsentatively. They have been chosen to represnt the whole of isolat Actynomycetes nine isolat which were successful isolated from soil around of Lindu Lake. The result of identification obtained three famalies of Actynomycetes consisted of four genus and six Sp.ecies. They were Streptomyces Sp.1, Steptomyces griseus, Streptomyces roseoticillatrus, Herbidospora cretaceae, Micromonospora, and Thermomonospora formonensis. There were many genus of Streptomyces from six isolats. Key words: Actynomycetes, identification, and Lindu Lake.
Pengujian Bakteri Coliform dan Escherichia Coli Pada Beberapa Depot Air Minum Isi Ulang di Kecamatan Palu Timur Kota Palu Muhammad Alwi; Sri Maulina
Biocelebes Vol. 6 No. 1 (2012)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study was aimed to know the quality of water refills in the district of east Palu, Central Sulawesi during periode of March until May 2012, with Purposive Random Sampling Method. Ten of fortyeight water refill was chosen as the sampling. This study was quantitatively with MPN (Most Probable Number) method for determination of Coliform used Lactosa Broth (LB) medium. In addition, was also conducted a qualitative test by testing of Escherichia coli used selective medium, there are Eosine Methylene Blue Agar (EMBA) and Mac Conkey Agar and then used Gram staining. The result showed tha the water refills in the district of east Palu were not for the requirements of SNI water with bacteriological test by 1 sampling (values of MPN Coliform about 4,3x101 (43 cell/ml)), and the requirements of SNI water are 9 sampling (values of MPN coliform about 3,0x100 (<3 cell/ml)) from all the sampling. But, for the testing of Escherichia coli is negative. Key words: Coliform, Escherichia coli, Drinking-Water refill station (DAMIU)
Keefektivan Metarhizium anisopliae Sebagai Agen Pengendali Hayati Terhadap Larva Lalat Musca domestica L. Muhammad Amiruddin; Umrah Umrah; Muhammad Alwi
Biocelebes Vol. 6 No. 1 (2012)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Research on the "effectiveness of the fungus Metarhizium anisopliae as a biological control agent against the larvae of the fly Musca domestica (L)” was done and focusingin determination of the effectiveness of this fungus as a biological control agent against Musca domestica larvae and to determine LC (Lethal Concentration) 50 and LC 90 on this biological control proceses. This study was designed in a completely randomized design consisting of 6 treatments and 3 replications. Arrangement of treatments i.e. the concentration of M. anisopliae in distilled water was; Po = distilled water without spore suspension, P1 = 5.1 × 1010 spores / mL, P2 = 2.0 × 1010 spores / mL, P3 = 1.0 × 1010 spores / mL, P4 = 5.1 × 109 spores / mL, and P5 = 2.0 × 109 spores / mL. Based on the observations, the mortality percentage rate was reached 93.33% as it in the treatment of spora concentration of 5.1 x1010 spores / mL.Probit analysis showed that LC50 was 8.3 x109 spores / mL with the range between the lower limit and the upper limit were 6.24 x109 spores / mL -11.43 x109 spores / mL. While the LC 90 was 5.0 x1010 spores / mL with the range of the lower limit until the upper limit were 2.8 x1010 spores / mL - 9.9 x1011 spores / mL. Key words: Metarhizium anisopliae, Musca domestica, effectiveness, mortality, biological control
Skrining Aspergillus Antagonis Terhadap Phythophthora palmivora Butler. Penyebab Penyakit Busuk Buah Kakao di Sulawesi Tengah Fitriani Husain; Umrah Umrah; Muhammad Alwi
Biocelebes Vol. 6 No. 2 (2012)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The study about “Screening of Aspergillus antagonistic to Phytopthora palmivora causing black pod disease of cocoa at Sulawesi Tengah” has been carried out during three months. The purpose of this study is how to getting the best of local isolats of Aspergillus sp. antagonistic that can be controlling of Phytopthora palmivora. The method of this study is exploring the local Aspergillus fungi as biological control of the P. palmivora causing black pod disease of cocoa in five Districts central of production kakao, such as Donggala District, Parigi Moutong, Poso, Morowali, and Buol. Parameter observed were antagonist test in vitro and pra in vivo on the cocoa. The result of this study was obtained fourteen isolats of Aspergillus sp namely are (1) Poso District: L-1, L-2, L-3 ; (2) Morowali District, L-4, L-5, L-6; (3) Donggala District, L-7, L-8, L-9; (4) Parimo District , L-10, L-11, L-12; (5) Buol District, L-13, L-14. All isolates of Aspergillus sp. were antagonistic in vitro on P. palmivora, but only six isolates, namely are L-1, L-10, L14, L-4, L-6, dan L-8 were have the best ability to controlling P. Palmivora on the cocoa pod. Key words: Aspergillus sp., antagonism, Phytophthora palmivora and Cocoa pod.
Uji Resistensi dan Sensitivitas Bakteri Salmonella thypi Pada Orang Yang Sudah Pernah Menderita Demam Tifoid Terhadap Antibiotik Nur Indang; Musjaya M. Guli; Muhammad Alwi
Biocelebes Vol. 7 No. 1 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This Research explores “The Resistance and Sensitivity Test of Salmonella thypi Bacteria of the People Who Had Ever Suffered from the Typhoid Fever toward the Antibiotic”, in which it was conducted on January to March 2013 at UPT Health Laboratory Palu Central Sulawesi. The objective of the research was to recognize the kind of antibiotics that is resistant and sensitive, and was to know the capacity zone of inhibition that was generated by the administering of antibiotic toward Salmonella thypi bacteria against people who had ever suffered from the typhoid fever. The researcher employed biochemical test, resistance and sensitivity test of Salmonella thypi bacteria toward the antibiotic. The findings shows that Salmonella thypi Bacteria is resistant toward the four kinds of antibiotics, they are 5.95 mm of Ampicillin, 6.9 mm of Amoxicillin, 7.9 mm of Cephalexin, and 8.1 mm of Chloramphenicol, and the Salmonella thypi Bacteria is sensitive toward four kinds of antibiotics, they are 21.35 mm of Cefatoxime, 22.6 mm of ceftazidime, 23.4 mm of Cefaperazone and 25.95 mm of Ceftriaxone. The most effective antibiotic to block the growth of Salmonella thypi bacteria is a Sefalosporin group of the third generation, they are Ceftriaxone, Cefoperazone, Ceftazidime, dan Cefatoxime. Key words: Typhoid Fever, Resistance and Sensitivity Test, Antibiotics, Salmonella thypi.
Uji Daya Hambat Ekstrak Buah Nanas (Ananas comosus L.Merr.), Salak (Salacca edulis Reinw.) dan Mangga Kweni (Mangifera odorata Griff.) terhadap Daya Hambat Staphylococcus aureus Endang Suerni; Muhammad Alwi; Musjaya M. Guli
Biocelebes Vol. 7 No. 1 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of this research was to investigate whether the extract of some fruits: pineapple (Ananas comosus L.Merr.), bark (Salacca edulis Reinw.), and Kweni mango (Mangifera odorata Griff.) has antimicrobial against the bacteria and how effective is the extract of pineapple (Ananas comosus L.Merr.), bark (Salacca edulis Reinw.), and kweni mango (Mangifera odorata Griff.). The bacteria of Staphylococcus aureus examined its growth is the bacteria purified from the common skin decease, that is, inflamed pimple. This research used Factorial RAL Design and further tested by employing Duncan Multiple Range Test (DMRT). If is found that the extract of pineapple, bark, and kweni mango can inhibit the growth of Staphylococcus aureus bacteria. The greatest inhibiting zone was the inhibiting zone produced by the extract of mango (Mangifera odorata Griff.) under the concentration of 100%. Key words: The extract of pineapple, bark, mango; inhibiting power, Staphylococcus aureus.
Studi Efektivitas Ekstrak Daun Sereh Wangi (Cymbopogon nardus L.) Sebagai Anti Fungi Candida albicans Eka Fitriani; Muhammad Alwi; Umrah Umrah
Biocelebes Vol. 7 No. 2 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The study was held on effectiveness of extract lemongrass leaf (Cymbopogon nardus L.) as a anti septic of Candida albicans. The study conducted as long as 3 months, from march to may 2013. Extracts was obtained by maceration method. This study aims to determine the extract can be prevent the growing of the Candida albicans, and to determine the effective concentration to prevent the growing of the fungus Candida albicans. This study is an experimental research with a completely randomized design. Bes trew diffusion method by a concentration of 25%, 50%, 75%, 100% and metronidazol liquid is used to determine the zone diameter inhibition in the five treatment and five replications. Inhibition zone diameter data were analyzed statistically ANOVA and Duncen Multiple Range Test (DMRT). The concentration stratified of extract effect on the zone diameter growth of Candida albicans. Based on the study, concentration at 75% is the best to be inhibited the growth of Candida albicans in conclusion, the extract is anti septic to the growth of Candida albicans. Keywords: Cymbopogon nardus L., Maceration, Candida albicans
Co-Authors Abdul Hafis Abdul Halik Abdul Hamid Shahab ABDULLAH, MUH. RUSLAN Agung Ramadhan Agung Sutrisno Ahmad Zaenal Wafik Akmal Hidayat Ali Rahman Aliyah Aliyah Andi Faisal Andi Faizah Makmur Andi Farhami Lahila M Andi Lindhemuthianingrum Anggie Angriani Nanik Ansar Ansar Asiah Azhari Hasibuan Bagus Singandaru, Adhitya Baiq Ismiwati Basri Rizak Bayu Nur Abdallah, Bayu Nur Busrah Caroline Adi Cahya Darul Ma'ruf Karim Dewa Nyoman Puspa Dwi Utami Puji Astuti Eka Fitriani Ellisa Salsanabila EMI SALMAH Endang Astuti Endang Suerni Eny Yuniati Eny Yuniaty Erma Erma Erna Febriani Eva Wegner Faschal Syawkani Wahid Fatahillah Alif Arif Fatmawati Fatmawati Fatmawati Fatmawati Felesia Angela R. Firman Saputra Jusri Fitratul Nakyah Fitriani Husain Gusti Ayu Arini Halijah Hamdana Hasriani Hasriani I Dewa Ketut Yuda S I Dewa Ketut Yudha S Ian Tubangsa Ikha Wahyuni Indah Ayu Lumintang Indah Sari B Ismail Ismail Jose Astro R. Kalbi Jafar Khairunnisa Rahmah Lanni Jurriah Latifah Merdekawaty Lenni Kusmiran Lina Anggraini Lodewyk Almando S. Luluk Fadliyanti Mahadin Shaleh Mahesa Putra Mansur Afifi Masrun masrun Misdar, Misdar Monica Anastasya Sianipar Muh. Arsal N. Maddo Muhajirin Muhammad Adam Muhammad Alim Fasieh Muhammad Amiruddin Muhammad Daffa V Muhammad Dzul Fadli Muhammad Isra Muhammad Maulana Fazri Munah Siregar Murni Ria Musjaya M. Guli Nely Handayani Niluh Budi Artini NUR ALFIYAH Nur Amalia Nur Asrinawaty Nur Hikma Nur Indang Nur Qadrianty Nurul Fadilah, Nurul Prakoso, Jatayu Hadi Pramudya Ari D. Puji Ghina Putu Karismawan Putu Karismawan Rahmi Wahyuni Rahmiati Rahmiati Ramadhanil Ramadhanil Ratnasari Dalimunte Resky Faradibah Suhab Resky Syahril Rezky Fauziah Nur Rini Sri Astutik Rizki Purnamasari Salfina Satria Abdillah Sirajuddin Siti Fatimah Siti Rodiah Lubis Sitti Rahmi Sri Maulina Syamsul Arifin Sysnindita Widya I Trian Fisman Adisaputra Umrah Umrah USWATUN HASANAH Vania Zita Vebri Sugiharto Yuni Arfatul Febria Yuni Tiara Yusuf Hasbullah