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Uji Resistensi dan Sensitivitas Bakteri Salmonella thypi Pada Orang Yang Sudah Pernah Menderita Demam Tifoid Terhadap Antibiotik Nur Indang; Musjaya M. Guli; Muhammad Alwi
Biocelebes Vol. 7 No. 1 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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This Research explores “The Resistance and Sensitivity Test of Salmonella thypi Bacteria of the People Who Had Ever Suffered from the Typhoid Fever toward the Antibiotic”, in which it was conducted on January to March 2013 at UPT Health Laboratory Palu Central Sulawesi. The objective of the research was to recognize the kind of antibiotics that is resistant and sensitive, and was to know the capacity zone of inhibition that was generated by the administering of antibiotic toward Salmonella thypi bacteria against people who had ever suffered from the typhoid fever. The researcher employed biochemical test, resistance and sensitivity test of Salmonella thypi bacteria toward the antibiotic. The findings shows that Salmonella thypi Bacteria is resistant toward the four kinds of antibiotics, they are 5.95 mm of Ampicillin, 6.9 mm of Amoxicillin, 7.9 mm of Cephalexin, and 8.1 mm of Chloramphenicol, and the Salmonella thypi Bacteria is sensitive toward four kinds of antibiotics, they are 21.35 mm of Cefatoxime, 22.6 mm of ceftazidime, 23.4 mm of Cefaperazone and 25.95 mm of Ceftriaxone. The most effective antibiotic to block the growth of Salmonella thypi bacteria is a Sefalosporin group of the third generation, they are Ceftriaxone, Cefoperazone, Ceftazidime, dan Cefatoxime. Key words: Typhoid Fever, Resistance and Sensitivity Test, Antibiotics, Salmonella thypi.
Uji Daya Hambat Ekstrak Buah Nanas (Ananas comosus L.Merr.), Salak (Salacca edulis Reinw.) dan Mangga Kweni (Mangifera odorata Griff.) terhadap Daya Hambat Staphylococcus aureus Endang Suerni; Muhammad Alwi; Musjaya M. Guli
Biocelebes Vol. 7 No. 1 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The purpose of this research was to investigate whether the extract of some fruits: pineapple (Ananas comosus L.Merr.), bark (Salacca edulis Reinw.), and Kweni mango (Mangifera odorata Griff.) has antimicrobial against the bacteria and how effective is the extract of pineapple (Ananas comosus L.Merr.), bark (Salacca edulis Reinw.), and kweni mango (Mangifera odorata Griff.). The bacteria of Staphylococcus aureus examined its growth is the bacteria purified from the common skin decease, that is, inflamed pimple. This research used Factorial RAL Design and further tested by employing Duncan Multiple Range Test (DMRT). If is found that the extract of pineapple, bark, and kweni mango can inhibit the growth of Staphylococcus aureus bacteria. The greatest inhibiting zone was the inhibiting zone produced by the extract of mango (Mangifera odorata Griff.) under the concentration of 100%. Key words: The extract of pineapple, bark, mango; inhibiting power, Staphylococcus aureus.
Studi Efektivitas Ekstrak Daun Sereh Wangi (Cymbopogon nardus L.) Sebagai Anti Fungi Candida albicans Eka Fitriani; Muhammad Alwi; Umrah Umrah
Biocelebes Vol. 7 No. 2 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The study was held on effectiveness of extract lemongrass leaf (Cymbopogon nardus L.) as a anti septic of Candida albicans. The study conducted as long as 3 months, from march to may 2013. Extracts was obtained by maceration method. This study aims to determine the extract can be prevent the growing of the Candida albicans, and to determine the effective concentration to prevent the growing of the fungus Candida albicans. This study is an experimental research with a completely randomized design. Bes trew diffusion method by a concentration of 25%, 50%, 75%, 100% and metronidazol liquid is used to determine the zone diameter inhibition in the five treatment and five replications. Inhibition zone diameter data were analyzed statistically ANOVA and Duncen Multiple Range Test (DMRT). The concentration stratified of extract effect on the zone diameter growth of Candida albicans. Based on the study, concentration at 75% is the best to be inhibited the growth of Candida albicans in conclusion, the extract is anti septic to the growth of Candida albicans. Keywords: Cymbopogon nardus L., Maceration, Candida albicans
Deteksi Suspek Tuberculosis Paru Pada Pekerja Tambang Poboya Palu Sulawesi Tengah Murni Ria; Musjaya M. Guli; Muhammad Alwi
Biocelebes Vol. 7 No. 2 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Research dealing with “ Detection of Suspected Pulmonary Tuberculosis of poboya gold mine workers at Palu, Central Sulawesi” was conducted from February to April 2013. It was aimed at knowing if there was any pulmonary tuberculosis at the reseach location. This research applied a discriptive method in order to describe the research sampple based on data obtained. The research result showed that there was one worker positively getting pulmonary tuberculosis with 2.5%, and there were 39 out of 40 workers as the sample being suspects of Pulmonary tuberculosis with 97,5%. Based on the findings, it can be known that the percentace of suspected pulmonary tuberculosis of Poboya gold mine workers at Palu, Central Sulawesi, was low. Keywords: Poboya, Deteksion, Suspek, Pulmonary Tuberculosis
Analisis Mikrobiologi Stik Kentang Goreng di Cafe Lesehan Talise Palu Rizki Purnamasari; Umrah Umrah; Muhammad Alwi
Biocelebes Vol. 7 No. 2 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Research entitling "Microbiological Analysis of Stik Potato Fry in Cafe Lesehan Talise Palu" have been executed in March until May 2013 as a mean to analyse the cemaran microbe of at stik potato fry in Cafe Lesehan Talise Palu and to know what stik potato fry the have fulfilled the Health Standard specified by BPOM by mikrobiologis. Result of research indicate that pursuant to microbiological analysis quantitatively is ALT And MPN of at sampel stik potato fry in Cafe Lesehan Talise Palu show 2 sampel which is not growed by colony, 6 sampel which is < 3,0 x 104 CFU/g, 1 incalculable sampel and 1 sampel with the result 3,1 x 104 CFU/g for the ALT of bacterium, while for the ALT of kapang obtained by 1 sampel which is not growed by the colony and 9 sampel which is < 3,0 x 104 CFU/g, and also for the ALT of khamir obtained by 3 sampel which is not growed by the colony, 6 sampel which is < 3,0 x 104 CFU/g and 1 sampel with the result 3,7 x 104 CFU/g. Most Probable Number (MPN) show 9 sampel which is 3,0 x 100 MPN/g (<3) and 1 sampel with the result 2,3 x 101 MPN/g namely Cafe C. From examination result 10 sampel stik potato fry knowable that 90% still fulfill the Health Standard specified by Director General BPOM of while 10% do not fulfill the Health Standard specified by Director General BPOM. Keywords: Microbiological Analysis, Stik Potato Fry, ALT and MPN
Deteksi Bakteri Coliform Dan Escherichia coli Pada Depot Air Minum Isi Ulang Di Kota Pasangkayu Kabupaten Mamuju Utara Sulawesi Barat Hasriani Hasriani; Muhammad Alwi; Umrah Umrah
Biocelebes Vol. 7 No. 2 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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This research entitle "Detect the bacterium of Coliform and Escherchia coli at depot of drinking water refill the diKota Pasangkayu of Sub-Province of Mamuju of North of West Sulawesi" have been executed in March until May 2013, in Biological Laboratory of Biological Majors Base of Faculty MIPA Tadulako University Palu. Target of this research to detect the bacterium of Coliform and Escherchia coli at depot of drinking water refill in Town of Pasangkayu of Sub-Province Mamuju North West Sulawesi and also determine the quality by mikrobiologis pursuant to Standard of Indonesia National (SNI). Method used in this research is Most Probable Number (MPN). Result of Research indicate that at fifth of depot which have in test, that is at test of MPN Coliform of there is three depot which are positive namely depot B for the BI OF standard water with the value 4 MPN/100 mL sampel, for the depot of C namely standard CI water with the value 150 MPN/100 mL sampel, CII irrigate the process by niali 23 MPN/100 mL sampel, CIII irrigate the gallon with the value MPN 21 MPN/100 mL sampel, while for the depot of D namely IN standard water show the value MPN 43 MPN/100 mL sampel. Pursuant to examination of Coliform Fecal and Escherchia coli expressed by all sampel is negativity. Keywords: Coliform, Escherchia Coli, and Drinking water refill.
Deteksi Bakteri Coliform DAN Escherichia coli Pada Minuman Es Jeruk Di Cafe Lesehan Pantai Talise Palu Ikha Wahyuni; Muhammad Alwi; Umrah Umrah
Biocelebes Vol. 7 No. 2 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Research entitle "Detect the Bacterium of Coliform and Escherichiacoli at Ices Beverage of Orange In Coastal Cafe Lesehan of Talise Palu" have been executed as a mean to detect and know the amount of bacterium Coiform and Escherichiacoli at ices beverage of orange in Coastal Cafe Lesehan of Talise Palu. This research have the character of descriptive, by using method of Standard Plate Count (SPC) And Most Probeble Number (MPN). Pursuant to research result from test SPC indicate that 50 % impure Cafe Lesehan because standard ineligibility of quality which is in specifying BPOM that is Cafe A of counted 1,1 x 104 CFU/ml, Cafe C of counted 4,3 x 104 CFU/ml, Cafe D of counted 1,5 x 104 CFU/ml, Cafe H of counted 1,8 x 104 CFU/ml. Examination of Method MPN from there are 30 % impure Cafe Lesehan by bekteri Coliform at ices beverage orange of because standard ineligibility of quality specified by BPOM that is at Cafe A of counted 143 x 101 MPN/ml, Cafe H of counted 2,33 x 101 MPN/ml, and Cafe J of counted 1,23 x 101 MPN/ml. Pursuant to examination of coliform fecal and test the Escherichia coli expressed by all sampel is negativity. Keywords: Coliform and Escherichia coli, ices beverage of orange, Cafe Lesehan.
IDENTIFIKASI BAKTERI FLORA NORMAL MUKOSA HIDUNG DAN SALIVA PADA PENAMBANG EMAS (TROMOL) DI KELURAHAN POBOYA KECAMATAN PALU TIMUR SULAWESI TENGAH Yuni Tiara; Muhammad Alwi; Musjaya M. Guli
Biocelebes Vol. 8 No. 1 (2014)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Research on the “Identification Of Normal Bacterial Flora of Nasal Mucosa and Salivary Gold (Tromol) Mining Village In The District Poboya Palu Central Sulawesi East” have been performed. Purpose of this study was to determine the type of bacterial that are normal flora in nasal mucosa and salivary poboya gold miners and todetermine the percentage of normal flora laboratory. Research method that was done in two stages field sampling and laboratory axamination. Sampling done by random. As for the number of samples that 30 consisting of 15 saliva and nasal mucosa 15. Result obtaind in the nasal mucosa was Enterobacter aerogenosa the proportion 18.1%, Staphyllococus aureus the proportion 36.4%, Proteus mirabilis the proportion 9.1%, Escherichia coli the proportion 9.1%, Enterobacter agglomerans the proportion 27.3%. Keywords : Identification, Normal Bacterial Flora, Nasal Mucosa, Salivary.
POTENSI LIMBAH CAIR TEMPE SECARA MIKROBIOLOGIS SEBAGAI ALTERNATIF PENGHASIL BIOGAS Nur Hikma; Muhammad Alwi; Umrah Umrah
Biocelebes Vol. 8 No. 1 (2014)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The research was about “Potential of Liquid Waste of Tempe Microbiologically as the alternative of Biogas Producer” had been conducted from July to September 2013 with the purpose of finding out of liquid waste of tempe microbiologically as the alternative of biogas producer and finding out the best concentration in media of liquid waste of tempe producer biogas and finding the best time for fermentation in producing biogas. The research method used is experimental which designed in the form of Complete Random Design (RAL), comprising 6 treatments and 3 times repetition. The research result shows that all treatment can produce biogas. The highest biogas volume is the treatment P5 (50% : 50%) which was as 128.10cm3. The best fermentation time occured on the third day (72 hours) up to the fourth day (96 hours) of fermentation. Keywords: Liquid Waste of Tempe, Microbiology and Biogas.
PEMANFAATAN LIMBAH CAIR TEMPE DAN LIMBAH CAIR TAHU DENGAN MENGGUNAKAN INOKULUM YANG BERBEDA UNTUK PRODUKSI BIOGAS Niluh Budi Artini; Muhammad Alwi; Umrah Umrah
Biocelebes Vol. 8 No. 1 (2014)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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This research was about “ The Usage of Liquid Waste of Tempe and Liquid Waste of Tofu by Using Different Inoculum to Produce Biogas”. The objective of the research was to use liquid waste of tempe and tofu by using different inoculum in producing biogas, finding out the interaction between the waste and microba inoculum in producing biogas, finding out the biggest amount of gas produced by liquid waste of tempe and tofu by using different inoculum in producing biogas and finding out the best time for fermentation in producing biogas. This research was designed by using Factorial Pattern of Complete Random Design (RAL) 2x2 comprising 4 treatments and 3 times repetition. The treatment examined was P1 )Liquid Waste of tempe + Rumen), P2 (Liquid Waste of tofu + Rumen), P3 (Liquid Waste of tempe + Rumen Contents), P4 (Liquid Waste of tofu + cow feces ) with the comparison of every treatment 50% :50%. Based on the research result, it shows that every treatment examined can produce biogas. The highest volume of examined treatment is P1 treatment (Liquid Waste of tempe + Rumen Contents) which was 57.15cm3. The best time for fermentation was the third day (72 hours). Keywords: Liquid Waste of tempe, Liquid Waste of tofu, Inoculum and Biogas.
Co-Authors , Suharsono Abdul Hafis Abdul Halik Abdul Hamid Shahab Abdul Rahim ABDULLAH, MUH. RUSLAN Adhitya Bagus Singandaru Agung Sutrisno Agus Purwito Ahmad Zaenal Wafik Akmal Hidayat Albar Albar Ali Rahman Aliyah Aliyah Andi Faisal Andi Lindhemuthianingrum Anggie Angriani Nanik Anna Hairani Ansar Ansar Asiah Azhari Hasibuan Baiq Ismiwati Basri Rizak Bayu Nur Abdallah, Bayu Nur Busrah Caroline Adi Cahya Dakhyar Nazemi Darul Ma'ruf Karim Dewa Nyoman Puspa Dwi Utami Puji Astuti Eka Fitriani Eli Anisa Ellisa Salsanabila EMI SALMAH Endang Astuti Endang Suerni Eny Yuniati Eny Yuniaty Erma Erma Erna Febriani Eva Wegner Faschal Syawkani Wahid Fatahillah Alif Arif Fatmawati Fatmawati Fatmawati Fatmawati Felesia Angela R. Firman Saputra Jusri Fitratul Nakyah Fitriani Husain Gusti Ayu Arini Hamdana Hasriani Hasriani I Dewa Ketut Yuda S I Dewa Ketut Yudha S Ian Tubangsa Ikha Wahyuni Indah Ayu Lumintang Indah Sari B Iqbal Nur Aswad Ismail Ismail Jatayu Hadi Prakoso Jose Astro R. Khairunnisa Rahmah Lanni Jurriah Latifah Merdekawaty Lina Anggraini Lodewyk Almando S. Luluk Fadliyanti Mansur Afifi Marida Sari Masrun masrun Misdar, Misdar Monica Anastasya Sianipar Muh. Arsal N. Maddo Muhajirin Muhammad Adam muhammad alfi syahrin Muhammad Alim Fasieh Muhammad Amiruddin Muhammad Daffa V Muhammad Dzul Fadli Muhammad Isra Muhammad Maulana Fazri Munah Siregar Murni Ria Musjaya M. Guli Nely Handayani Niluh Budi Artini NUR ALFIYAH Nur Asrinawaty Nur Hikma Nur Indang Nur Laili Nurul Fadilah, Nurul Pramudya Ari D. Puji Ghina Putu Karismawan Putu Karismawan Rahmi Wahyuni Rahmiati Rahmiati Ramadhanil Ramadhanil Ratnasari Dalimunte Rezky Fauziah Nur Rini Sri Astutik Rizki Amaliah Rizki Purnamasari Safitri, Nursan Saleh, Nursahdi Salfina Satria Abdillah Sirajuddin Siti Fatimah Siti Rodiah Lubis Sitti Rahmi Sri Maulina Syamsul Arifin Sysnindita Widya I Thoibah Trian Fisman Adisaputra Umrah Umrah USWATUN HASANAH Vania Zita Vebri Sugiharto Yuni Arfatul Febria Yuni Tiara Yusuf Hasbullah