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All Journal HAYATI Journal of Biosciences Agrikultura Jurnal Ilmu Dasar AL KAUNIYAH Planta Tropika Jurnal Ilmiah Rekayasa Pertanian dan Biosistem Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosaintifika: Journal of Biology & Biology Education Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Pengabdian Magister Pendidikan IPA Reswara: Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun International Journal on Food, Agriculture and Natural Resources (IJFANRES) Golden Ratio of Mapping Idea and Literature Format Journal of Applied Agricultural Science and Technology Karimah Tauhid Jurnal Cakrawala Akademika
Ratnasari, Tri
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Journal : AL KAUNIYAH

 1 
Respon IAA dan BAP Terhadap Multiplikasi In Vitro Tunas Tanaman Krisan Varietas Suciono (Chrysanthemum indicum L) Restanto, Didik Pudji; Nafiah, Nur Lailin; Fanata, Wahyu Indra Duwi; Ratnasari, Tri; Firgiyanto, Refa
Al-Kauniyah: Jurnal Biologi Vol. 17 No. 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i2.1.27417

Abstract

AbstrakTanaman krisan varietas Suciono merupakan tanaman hias yang potensial dikembangkan dan diminati oleh penduduk dunia, yang ditunjukkan dengan permintaan pasar terhadap tanaman krisan meningkat sepanjang tahun. Hal ini menyebabkan perbanyakan krisan secara in vitro sangat penting untuk dilakukan. Penelitian ini bertujuan untuk mengetahui pengaruh Indole Acetic Acid (IAA) dan Benzly Amino Purine (BAP) terhadap multiplikasi tunas krisan varietas Suciono. Desain penelitian ini menggunakan Rancangan Acak Lengkap dengan satu faktor yakni kombinasi hormon IAA dan BAP yang 9 kombinasi, yakni 0 mg/L +  1,5 mg/L; 0 mg/L + 2 mg/L; 0 mg/L + 2,5 mg/L; 0,5 mg/L + 1,5 mg/L; 0,5 mg/L + 2 mg/L; 0,5 mg/L + 2,5 mg/L; 1 mg/L + 1,5 mg/L; 1 mg/L + 2 mg/L; dan 1 mg/L + 2,5 mg/L dimana setiap kombinasi diulang sebanyak 3 kali. Variabel yang diamati meliputi analisis histologis kalus, waktu muncul tunas, jumlah tunas, tinggi tunas, dan jumlah daun. Hasil analisis ragam atau ANOVA menunjukkan bahwa kombinasi IAA 0 mg/L dan BAP 2 mg/L merupakan kombinasi konsentrasi terbaik untuk multiplikasi tunas. Penambahan BAP 2 mg/L mampu menghasilkan persentase muncul tunas. 100%, jumlah tunas 3 per-eksplan, tinggi tunas 7 cm, dan jumlah daun sebanyak 16 helai.AbstractThe variety Suciono of chrysanthemum is a potential ornamental plant that is in demand by the world's population, as indicated by the increasing market demand for chrysanthemum plants throughout the year. This  has led to the importance of in vitro propagation of chrysanthemum. The aim of the this study is to determine the effect of Indole Acetic Acid (IAA) and Benzly Amino Purine (BAP) on the multiplication of chrysanthemum shoots of the Suciono variety. The design of this study used nodus explants, treated with a combination of IAA hormones and BAP. It is make 9 combinations, and then the combinations of hormones is 0 mg/L + 1,5 mg/L; 0 mg/L + 2 mg/L; 0 mg/L + 2,5 mg/L; 0,5 mg/L + 1,5 mg/L; 0,5 mg/L + 2 mg/L; 0,5 mg/L + 2,5 mg/L; 1 mg/L + 1,5 mg/L; 1 mg/L + 2 mg/L; and 1 mg/L + 2,5 mg/L.  The observed variables included histological analysis of callus, time of shoot emergence,  number of shoots, shoots height, and number of leaves. The results of the analysis showed that the treatment with 0 mg/L IAA and 2 mg/L BAP was the best concentration for shoots multiplication. The addition of 2 mg/L BAP was able to produce a percentage of shoots emergence of 100%, 3 shoots per explant, shoots height of 7 cm and 16 leaves.
Co-Authors Agung Sih Kurnianto Ahmad Ilham Tanzil Aji, Seto Purnomo Amany, Alfiyyah Nur Ana, Daniar Alfi Arif Mohammad Siddiq Arif Mohammad Siddiq Ariyono, Alief Rizky Arum, Ayu Puspita Asshidiqie, Wahyu Pratama Bambang Sugiharto Budi Kriswanto, Budi Cahyono, Haris Denny Hermawan, Denny Dewi, Nilasari Dwi Setyati Dyah Ayu Savitri Euis Salbiah Fadhillah, Dewi Nur Fanata, Wahyu Indra Duwi Firdaus, Anggun Sausan Gustina, Reza Handayani, Etty Hari Purnomo Hari Setiyowati Hari Sulistyowati Hasanah, Ela Apriliyanti Ika Purnamasari Intan Kartika Setyawati Khamidah, Khusna Khowatini, Husnul Khozin, Mohammad Nur Luh Putu Suciati Magfiroh, Illia Seldon Meliala, Susan Barbara Patricia Sembiring Mochammad Wildan Jadmiko Mohammad Ali Mudhor Muhammad, Adibillah Muhtadi, Much. Misbah Nafiah, Nur Lailin Neliana, Intan Ria Nikmah, Nasilatun Nilasari Dewi Nisa, Aminatun Noer Novijanto Novitasari , Adin Nur Widodo Nurcharisma, Adinda Parawita Dewanti Parawita Dewanti Patricia SM, Susan Barbara Prasojo, Sri Irawan Laras Prayoga, Mohammad Candra Puji Rahayu Putri, Romadhona Destria Raharja, Ajung Gilang Putra Ramadhani, Rifki Fahrisal Ramdani, Faisal Tri Refa Firgiyanto Restanto, Didik Pudji Ristiyana, Suci Setiyono Setiyono Siti Rohimah Soviana, EIida Sri Andriani Su’udi, Mukhamad Sulistiyowati, Hari Syafina Pusparani Tri Handoyo TRI HANDOYO Tri Wahyu Saputra Ummi Sholikhah Utami, Sri Rejeki Wahyu Nurkholis Hadi Syahputra Yagus Wijayanto Yulianto, Roni Yusuf Rachmandika Zakiah, Ratu Ulfah