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Marhawati, Marhawati
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Journal : Journal La Lifesci

 1 
Optimization of PCR Analysis Based on Start Codon Targeted Markers (SCoT Markers) for Identification of Genetic Variation of Seaweed from Central Sulawesi Santoso, Tri Joko; Husni, Ali; Nugroho, Kristianto; Ya’la, Zakirah Raihani; Dewi, Triyani; Marhawati, Marhawati; Maemunah, Maemunah; Rosyida, Eka; Ndobe, Samliok
Journal La Lifesci Vol. 5 No. 1 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i1.1062

Abstract

Seaweed is a fishery commodity that has high economic value because it contains carrageenan which can be used in the pharmaceutical, food, cosmetics, and industrial fields. Central Sulawesi Province is the second largest seaweed producer in Indonesia after South Sulawesi Province. The genetic diversity of seaweed in Central Sulawesi has not been studied much. Therefore, molecular-based characterization activities to identify the genetic variation of seaweed in the province are urgently needed. The purpose of the study was to optimize PCR techniques based on targeted start codon markers (SCoT markers) to identify the genetic diversity of seaweed accessions from Central Sulawesi Province. The results showed that the parameters for seaweed PCR amplification have been optimized. Of the fifteen SCoT primers, 10 of them can work to amplify seaweed DNA collected from several regions in Central Sulawesi. This is shown by the production of PCR result amplicons. PCR results also indicate polymorphism from the seaweed samples tested, although some seaweed samples have not been successfully amplified. The parameters of the PCR technique still need to be improved, especially the concentration of DNA prints, so that valid PCR results will be obtained for use in identifying seaweed genetic diversity.
Co-Authors abu, ilham Achmad Noerkhaerin Putra Adiningsih S, Sri Hutami Adys, Himaya Praptani Aep Saefullah Agus Syam Ahmad Faqhruddin Abdur-Rabb Akram, Andi Muhammad Luthfi Alfatiha, Ainaya alfira, alfira Ali Husni Ampa, A. Tenri Amrullah, Andi Ananda, Alda Andi Tenri Ampa Andi Tenri Ampa Andika Isma Anna Lidiyawati Annisa, Andi Asmayanti ASMAYANTI, ASMAYANTI Asti Handayani, Andi Azizah, A Azrul Dermawansyah Darwipat Dewi Fatmarani Surianto Dinar, Muhammad Eka Rosyida Erwina Erwina, Erwina Grison Masiku, Arjuna Agung Imam Prawiranegara Gani Indriyani Irwandi Jumriani, Jumriani KRISTIANTO NUGROHO, KRISTIANTO Kurnia M. Miftach Fakhri Maemunah Maemunah Muh Zakkar Ruslan MUHAMMAD DINAR Muhammad Hasan Muhammad Hasan MUHAMMAD IHSAN Muhammad Ihsan Said Ahmad Muhammad Jufri Muhammad Rakib Multhazam, Riana MUSTARI Mustari Mustari Nudiana, Nudiana Nur Arisah Nur Halim NUR ISMI Nuraeni Nuraeni Nurhidayah, AM. Fatwa Nurhidayati Nurjannah Nurjannah Nurjannah Nurlinda Parawansa, Dirgah Agum Putra, Trimadi Jaya Raflianto, Raflianto Rahmad Risan Rahman, Nur Delima Novita Rahmatullah Rini Oktavera Said, Muh Ihsan Said, Muh. Ihsan Samliok Ndobe SAMSYU RIJAL Shadry Andriani Sitti Mawaddah Umar Sosiawati, Endang Sapta Hari Sri Budyartati Sri Wahyuni Sri Wahyuningsih Sry Astuti, Sry Sumiati Tahir Sutiharni Syam, Agus Syamsu Rijal Syamsul Rijal Tadampali, Andi Caezar To Thamrin Tahir Thamrin Tahir, Muhammad Ilyas TRI JOKO SANTOSO Triyani Dewi Tuti Supatminingsih Valentino Aris, Valentino Wahyu Hidayat Wahyuningsih , Sri Zakirah Raihani Ya’la