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All Journal Current Biochemistry JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Journal Of Nursing Practice Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Amerta Nutrition Jurnal Entomologi Indonesia Pekobis : Jurnal Pendidikan, Ekonomi, dan Bisnis SELL (Scope of English Language Teaching, Linguistics, and Literature) Journal Buletin Poltanesa EKONOMIKA45 Jurnal Multidisiplin Madani (MUDIMA) Journal of Innovation Research and Knowledge Jurnal Pelayanan dan Pengabdian Masyarakat Indonesia Innovation, Theory & Practice Management Journal Perigel: Jurnal Penyuluhan Masyarakat Indonesia Jurnal manajemen informasi kesehatan Kanigara Jurnal Pengabdian Masyarakat Jurnal Lentera Kesehatan Masyarakat (JLKM) Poltekita: Jurnal Ilmu Kesehatan Advances in Environmental Innovation (ANEVA) Jurnal Penelitian Hasil Hutan Journal of Social And Education Research Professional Evidence-based Research and Advances in Wellness and Treatment
Sulistiyani
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Journal : current biochemistry

 1 
Antioxidant Activity of Telang (Clitoria ternatea L.) Extract in Inhibiting Lipid Peroxidation Ukhradiya Magharaniq Safira Purwanto; Kamaratih Aprilia; Sulistiyani
Current Biochemistry Vol. 9 No. 1 (2022)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.9.1.3

Abstract

Butterfly pea (Clitoria ternatea L.) has been widely used as a healthy drink as well as traditional medicine. This study aimed to test the antioxidant activity of C. ternatea aqueous extract based on its ability to inhibit lipid peroxidation (in vitro) using different parts and extraction methods. Extraction was carried out on flowers, leaves, and roots. The leaves and roots were extracted by boiling technique. Flowers were extracted in 3 ways: boiling, maceration, and brewing. Antioxidant activity was determined based on phenolic content, flavonoid content, and antioxidant test using the TBA method. The highest phenolic and flavonoid levels were shown by the aqueous extract of C. ternatea leave of 57.51 mg GAE/g and 1.50 mg EK/g, respectively. The highest levels of flavonoid were from flower parts treated with boiling technique (0.88 mg EK/g), followed by brewed flowers and macerated flowers. The highest antioxidant activity was obtained from flowers that were boiled for 30 minutes with a percentage inhibition of 94%. Based on the method of extraction and part of the plant, all extracts of C. ternatea have similar antioxidant potential.
Combination of Pulai Leaf (Alstonia scholaris (L.) R. Br.) and Papaya Leaf (Carica papaya L.) Water Extract as HMG-CoA Reductase Inhibitor Sulistiyani; Andrianto, Dimas; Mariati, Sri
Current Biochemistry Vol. 11 No. 1 (2024)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.11.1.3

Abstract

Heart disease has become the leading cause of death in the world in the last 15 years. This disease has several primary risk factors, one of which is hypercholesterolemia. Cholesterol biosynthesis in the liver is influenced by the activity of the rate-limiting enzyme, HMG-CoA reductase. Traditional herbal medicine provides alternative treatment for hypercholesterolemia. Both pulai and papaya leaves were reported as HMG-CoA reductase inhibitors. There has been very limited study however, on the efficacy of a mixture of aqueous extracts of the two in inhibiting HMG-CoA reductase. This study aims to determine the activity of a mixture of water extracts of both pulai and papaya leaves. The activity of the enzyme HMG-CoA reductase was measured by Abcam® kit No. Ab204701 by spectrophotometric method at 334 nm. The results showed that the three extracts of the combination of pulai and papaya leaves had four times better inhibitory action than the single extract (p0.05). The results of the phytochemical literature study showed that the aqueous extract contained flavonoids, alkaloids, tannins, and saponins
Kandungan Nutrisi dan Daya Inhibisi α-glukosidase Ekstrak Daging Buah Salak Sidempuan (Salacca sumatrana) Safithri, Mega; Ritonga, Aprilita Putri Defan; Indariani, Susi; Dwicesaria, Maheswari Alfira; Sulistiyani; Hasim
Current Biochemistry Vol. 11 No. 1 (2024)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.11.1.1

Abstract

Penelitian terhadap pemanfaatan salak khususnya salak Sidempuan sebagai inhibitor α-glukosidase belum dilakukan. Penelitian ini bertujuan mengukur kadar proksimat (air, abu, protein kasar, lemak kasar, serat kasar, dan karbohidrat), menganalisis komponen fitokimia, kandungan total fenolik dan aktivitas penghambatan α-glukosidase pada ekstrak air dan etanol daging buah salak Sidempuan. Kadar total fenol diukur menggunakan metode Folin Ciocalteu. Aktivitas penghambatan α-glukosidase diukur menggunakan substrat pNPG. Kadar karbohidrat, air, abu, lemak kasar, protein kasar, dan serat kasar berturut-turut sebesar 86.35%, 6.57%, 3.60%, 1.44%, 1.24%, dan 0.82%. Kedua ekstrak mengandung senyawa flavonoid dan saponin. Ekstrak etanol 70% daging buah salak Sidempuan memiliki kandungan total fenol sebesar 10.6 mg GAE/g ekstrak, lebih tinggi dibandingkan ekstrak air sebesar 5.55 mg GAE/g esktrak. Ekstrak etanol daging buah salak Sidempuan memiliki konsentrasi inhibisi (IC50) sebesar 13.69 mg/L. Konsentrasi ini lebih kecil dibandingkan ekstrak air yaitu 4160.56 mg/L, namun masih lebih besar dibandingkan akarbosa yaitu 1.15x10-3 mg/L sebagai kontrol positif.
Analisis Profil Metabolit Sekunder Ekstrak Etanol Brokoli (Brassica oleracea var. italica) pada Pemupukan Biokonversi Maggot Black Soldier Fly Purwanto, Ukhradiya Magharaniq Safira; Sulistiyani; Azizah, Anisa Nur Wahida; Rosyidah, Rara Annisaur
Current Biochemistry Vol. 12 No. 2 (2025)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.12.2.3

Abstract

Brokoli (Brassica oleracea var. italica) merupakan sayuran fungsional yang diketahui mengandung berbagai senyawa bioaktif dengan potensi nutrisi dan farmakologis yang penting. Penelitian ini bertujuan untuk memprofilkan senyawa bioaktif ekstrak brokoli yang dibudidayakan dengan perlakuan pemupukan berbeda, yaitu kontrol, pupuk kandang, dan pupuk biokonversi maggot black soldier fly (BSF). Sampel brokoli diekstraksi menggunakan etanol 80%, kemudian dianalisis dengan LC-MS/MS untuk mengidentifikasi dan mengkuantifikasi komposisi metabolit. Hasil penelitian menunjukkan bahwa perlakuan pupuk biokonversi maggot menghasilkan pertumbuhan tanaman yang lebih baik, meliputi tinggi tanaman, jumlah daun, biomassa segar dan kering, serta persentase berbunga dibanding perlakuan lain. Selain itu, perlakuan ini juga menghasilkan metabolit yang lebih beragam dengan kelimpahan lebih tinggi, terutama golongan fenolik, terpenoid, piridazin, dan indol. Beberapa senyawa utama yang teridentifikasi antara lain Indole-3-Propionic Acid dan N(1),N(8)-Bis(Coumaroyl)Spermidine(1+), serta turunan klorofil seperti pyropheophorbide-a yang ditemukan dengan kelimpahan signifikan. Temuan ini menunjukkan bahwa pupuk biokonversi maggot BSF mampu meningkatkan pertumbuhan sekaligus profil metabolit sekunder brokoli, sehingga berpotensi sebagai strategi pemupukan ramah lingkungan untuk meningkatkan kualitas nutrisi dan fungsional tanaman ini. 
Co-Authors Abdjad Asih Nawangsih Abdul Azis Akbar Aditya Pratama Agata, Annisa agustin, anggi_14 Amsar Ari Tri Wanodyo Handayani Arini Hidayah, Arini Aris Mardiono Aris Mardiyono Azizah, Anisa Nur Wahida Caecilia Sri Haryanti Camilius Daramusseng, Andi Denok Julianingsih Dian Purnamasari DIMAS ANDRIANTO Dondin Sajuthi Dwicesaria, Maheswari Alfira Efendi, Tri Achmad Elyda Akhya Afida Misrohmasari Firdaus, Muhamad Fajar Fitriani, Janiyah Intan Gita Sugiarti Hasim Hikmah Indah, Dewi Rosaria Indariani, Susi Irma Herawati Suparto Kamaratih Aprilia Kiswaluyo Lalu Guntur Payasan Lamria Situmeang Mega Safithri Mei Rianita Sinaga Misrohmasari, Elyda Muchayatin Muhammad Auliya Rahman Mursid Raharjo Ni Luh Putu Maharani Eka Putri Nikie Astorina Yunita Dewanti Ninef, Violin Irene Novitasari, Ade Nur Rahman, Arif Nurhidayah, Sri Nurwinda Sari, Nova Pamase, Ulrico Adnan Parera, Magdalena Junu Payasan, Lalu Guntur Prihantini, Apsari Fajar Purwanto, Ukhradiya Magharaniq Safira Putri, Nur Aini Saura Rachmawati, Septi Nur Rahayu, Endah Sri Retno Murwani Ritonga, Aprilita Putri Defan Roedy Budirahardjo Rosaria Indah, Dewi Rosyidah, Rara Annisaur Samsir Sigiro, Ria Heni Silwana, Amalia Soenarmi, Fransisca R. Sri Mariati Subaidah Suhartono Sumarmi Suparjo Surartono Dwiatmoko Widayanti, Evi Yuniarto, Galuh Yusniar Hanani Darundiati Zuraida