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All Journal Buletin Peternakan METAMORFOSA Journal of Biological Sciences Biotropika Biology, Medicine, & Natural Product Chemistry Journal of Tropical Biodiversity and Biotechnology BIOMA : Jurnal Biologi Makassar Jurnal Biodjati Jurnal Penelitian Pendidikan IPA (JPPIPA) BIOEDUSCIENCE Jurnal Biologi Tropis Justek : Jurnal Sains Dan Teknologi Biosel: Biologi Science and Education Jurnal Pengabdian Magister Pendidikan IPA Biota BIO-EDU: Jurnal Pendidikan Biologi Jurnal Abdidas Koordinat : Jurnal Pembelajaran Matematika dan Sains Bioscientist : Jurnal Ilmiah Biologi Media Eksakta Journal Evaluation in Education (JEE) AGRI-TEK : Jurnal Penelitian Ilmu-ilmu Eksakta Interdisciplinary International Journal of Conservation and Culture Journal of Biology Science and Education
Manap Trianto
Departement Of Biology Education, Faculty Of Teacher Training And Education, Tadulako University
Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Burung Gosong Filipina (Megapodius cumingii) di Sulawesi Tengah: Variasi Haplotipe dan Hubungan Filogenetik Berdasarkan Gen EEF2 Novitasari; Budiarsa, I Made; Suleman, Samsurizal M.; Kundera, I Nengah; Jayanti, Zulaikhah Dwi; Trianto, Manap
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.17540

Abstract

This study aims to describe the variation in haplotype distribution and phylogenetic relationships of the Philippine scrub fowl based on the EEF2 gene. Eggshell samples were obtained from Tolitoli and Luwuk, Central Sulawesi. DNA isolation followed the gSYNCTM Extraction Kit protocol, and the amplification process was carried out in 38 cycles, including the pre-denaturation stage (95°C, 5 minutes), denaturation stage (95°C, 45 seconds), annealing stage (53°C, 40 seconds), elongation stage (72°C, 1 minute), and post-elongation stage (72°C, 10 minutes), followed by sequencing. Sequence editing was performed using GeneStudio, sequence alignment and phylogenetic tree reconstruction using MEGA 11, and haplotype network reconstruction using DnaSP and PopART. The results showed that the haplotype network based on the EEF2 gene formed three haplotypes, with the population from Tolitoli having a widespread haplotype, while the population from Luwuk had a specific haplotype. In addition, based on the results of phylogenetic tree reconstruction, it was found that the Philippine white-eye birds from Tolitoli and Luwuk were in the same clade but there was genetic variation between populations.
Analysis of Nutritional Composition and Antioxidant Activity of Yogurt Enriched with Butterfly Pea (Clitoria ternatea L.) Extract Windarsih, Yulia; Budiarsa, I Made; Trianto, Manap; Akram, Akram; Ashari, Abdul
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.831-841

Abstract

The growing demand for functional foods has encouraged the development of dairy products enriched with natural bioactive ingredients to improve nutritional value and health benefits. This study aimed to analyze the nutritional composition and antioxidant activity of yogurt enriched with butterfly pea (Clitoria ternatea L.) extract. Yogurt was prepared from fresh goat milk with varying concentrations of butterfly pea extract and evaluated for protein, fat, carbohydrate, moisture, ash, vitamin C contents, antioxidant activity, and sensory acceptance. Proximate analysis, UV–Vis spectrophotometry, and the DPPH method were used for chemical determinations, while a hedonic test assessed color, taste, aroma, and texture. Results showed that increasing butterfly pea extract significantly decreased protein and fat contents but increased vitamin C and antioxidant activity, with the highest values observed in yogurt containing 60% extract and 4% formulation (N60K2). Moisture and ash contents were not significantly affected. Sensory evaluation indicated that yogurt with 60% extract and 2% formulation (N60K1) was preferred regarding color, taste, aroma, and texture. Considering compliance with the Indonesian National Standard (SNI) and consumer acceptance, N60K1 was the optimal formulation. The findings suggest that butterfly pea extract can enhance the functional properties of yogurt, particularly its antioxidant potential, while maintaining acceptable sensory quality. This provides insights for developing functional dairy products with natural plant-based bioactives.
Celery Plant Growth Response Given Organic Fertilizer with Banana Peel and Coconut Water Alibasyah, Lestari MP; Ningsi, Ririn Apriani; Nurfadila A, Nurfadila A; Trianto, Manap
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7483

Abstract

Celery (Apium graveolens L.) is a vegetable that has many health benefits because it contains nutrients and antioxidants that makes it popular among the public, but its production is relatively low. One way to improve the quality and quantity of celery plants is to provide liquid organic fertilizer (LOF) of banana peel and coconut water which contains essential nutrients such as calcium, phosphorus, nitrogen, potassium, magnesium, sodium, sulfur, sugar, protein, and two hormones. namely cytokinins and auxins which are needed in the growth of the plant. The study aims to analyze the growth response of celery plants given liquid organic fertilizer (LOF) from banana peel and coconut water. The type of research is an experiment with a complete randomized design (CRD) consisting of 7 treatments, namely (P0 = Control, P1 = 10% banana peel, P2 = 15% banana peel, P3 = 20% banana peel, P4 = 10% coconut water, P5 = 15% coconut water, and P6 = 20% coconut water), each treatment is repeated 6 times with a total sample of 42. The observed variables were plant height, number of leaves, and number of tillers measured at 6 WAP, 8 WAP, 10 WAP, and 12 WAP. Data analysis used ANOVA followed by a 5% significant BNT test with the help of the SPPS 25 program. The results of the research showed that the best growth was at the age of 12 WAP with a concentration of 20% organic fertilizer and banana waste organic fertilizer was more effective than coconut water in increasing the response of celery plant growth.
Flavonoid and Alkaloid Content of Red Onion Skin Extract (Allium cepa L.) Windarsih, Yulia; Masrianih; Trianto, Manap
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8578

Abstract

Red onion skin (Allium cepa L.) is often discarded as waste, despite being rich in flavonoids and alkaloids, which possess antioxidant, anti-inflammatory, antimicrobial, and anticancer properties. This study aims to determine the flavonoid and alkaloid content of red onion skin extract using reflux and Soxhlet extraction methods with 96% ethanol as the solvent. The extraction was performed using reflux and Soxhlet techniques, followed by UV-Vis spectrophotometry to quantify flavonoid and alkaloid content. A quercetin standard curve was used to determine flavonoid levels, while a caffeine standard curve was applied for alkaloid content analysis. The Soxhlet method yielded a higher flavonoid content (108.21 mg/g or 10.82%) compared to the reflux method (105.55 mg/g or 10.55%). In contrast, the reflux method resulted in a higher alkaloid content (169.56 mg/g or 16.95%) than Soxhlet extraction (167.0 mg/g or 16.70%). These differences are attributed to the continuous solvent circulation in Soxhlet extraction, which enhances flavonoid solubility, while the lower temperature in the reflux method prevents alkaloid degradation. The findings suggest that Soxhlet extraction is more effective for flavonoid extraction, whereas reflux extraction is better for alkaloid extraction. Given their significant bioactive properties, red onion skin extracts have potential applications in the pharmaceutical, nutraceutical, and food industries. Future research should focus on optimizing extraction techniques and conducting toxicological and pharmacological studies to explore the therapeutic potential of red onion skin extract.
Molecular Identification of Lepidopteran Predators of Coccidae on Coffee Plants (Coffea canephora) in Sigi Regency Budiarsa, I Made; Trianto, Manap; Adnan, Bahana Aditya
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.983-992

Abstract

This study aimed to identify the species of Lepidopteran predators that prey on soft scale insects (Coccidae) found on coffee plants in Sigi Regency using molecular techniques. DNA was extracted using the modified CTAB method (Doyle & Doyle, 1990). PCR amplification targeted the COI gene using universal primers LCO1490 and HCO2198 with a GeneAmp PCR System 9700. Visualization was done using 1% agarose gel and UV-transilluminator. Sequencing was conducted externally. Data were analyzed using BioEdit 7.2.5, BLAST, BOLD Systems, and MEGA11 software. The DNA sequence of the predator sample showed 97.91% similarity to Autoba rubra based on GenBank and 97.59% in BOLD Systems. Phylogenetic analysis confirmed a close genetic relationship with A. rubra, distinct from Eublemma. Morphological similarities with Eublemma were misleading, highlighting the accuracy of molecular identification. This study is the first to confirm the identity of a Lepidopteran predator of coffee scale insects in Indonesia using molecular techniques, revealing its close relation to Autoba rubra. The results correct previous morphological misidentifications and contribute new data on predator diversity in biological control contexts.
Analisis Filogenetik Apis dorsata (Fabricius 1793) Bedasarkan Gen Chytochrome Oxidaxe I (COI) Delima, Nur; Masrianih, Masrianih; Trianto, Manap; Windarsih, Yulia; Buntu, Amalia; Dhafir, Fatmah
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14899

Abstract

Apis dorsata is a giant honeybee species distributed across South and Southeast Asia. Phylogenetic analysis is used to illustrate the evolutionary relationships of organisms through a phylogenetic tree. This study aims to describe the genetic relationships of A. dorsata based on the cytochrome oxidase I (COI) gene. Sampling was conducted using an exploratory method in various regions, followed by DNA isolation, DNA amplification, sequencing, and bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The results show that phylogenetic analysis using the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that clearly depicts evolutionary relationships. A. dorsata from Central Sulawesi is more closely related to other A. dorsata species within the same internal cluster, while other species are separated. These findings reveal a clear pattern of genetic relationships among A. dorsata populations.
Analisis Filogenetik Cryptic species Apis cerana Fabricius, 1793 Berdasarkan Gen 16S rRNA Puspitasari, Amira; Budiarsa, I Made; Ashari, Abdul; Dhafir, Fatmah; Windarsih, Yulia; Trianto, Manap
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14967

Abstract

Apis cerana is a member of the Insecta class grouped in the order Hymenoptera. Phylogenetic analysis is used to describe the kinship of living things through phylogeny trees. This study aims to describe the phylogenetic relationship of cryptic species in Apis cerana based on the 16S rRNA gene. Sampling use the cruising method in various regions, the DNA isolation, DNA amplification, sequencing, followed by bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The result showed that phylogenetic analysis with the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates clear evolutionary relationship. A. cerana from Central Sulawesi is more closely related to other A. cerana species in the same clade, while other species are separated. The result of this study show a clear pattern of kinship between A. cerana populations.
Analisis Filogenetik Lebah Madu Endemik Sulawesi Apis nigrocincta Smith, 1860 Berdasarkan Gen 16S rRNA Hidyanisa, Dhea; Budiarsa, I Made; Ashari, Abdul; Trianto, Manap; Nurdin, Musdalifah; Windarsih, Yulia
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14968

Abstract

Apis nigrocincta is an endemic honey bee that is only found in two regions in Indonesia, namely the Sangihe Islands and Sulawesi Islands. Phylogenetic analysis is used to describe the phylogenetic relationship of Sulawesi endemic honey bee A. nigrocincta based on the 16S rRNA gene. Sampling using the cruising method in various regions, then DNA isolation, DNA amplification, sequencing, and continued bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The results showed that phylogenetic analysis with the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates clear evolutionary relationships. A. nigrocincta from Central Sulawesi is more closely related to other A. nigrocincta species in the same clade, while other species are separated. The results of this study show a clear pattern of kinship between A. nigrocincta populations. 
Analisis Filogenetik Lebah Tukang Kayu Xylocopa appendiculata Smith, 1852 Berdasarkan Gen Cytochrome Oxidase 1 (CO1) Rahayu, Irma Septi; Budiarsa, I Made; Ashari, Abdul; Trianto, Manap; Masrianih, Masrianih; Rafiqa, Rafiqa
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14969

Abstract

Xylocopa appendiculata  is an insect from the order Hymenotera and has bright yellow morphological characteristics on the thorax, while the rest of the body is black. Phylogenetic analysis is used to describe the kinship of living things throughh phylogeny trees. This study aims to describe the phylogenetic relationship of carpenter bees Xylocopa appendiculata based on the cytochrome oxidase 1 (CO1). DNA isolation, DNA amplification, sequencing, followed by bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The results showed that phylogenetic analysis using Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates a clear evalutionary relationship X. appendiculata from Central Sulawesi is more closely related to other X. appendiculata species in the same clade. The results of this study show a clear kinship pattern between populations of X. Appendiculata.
Identifikasi Pakan Lebah Madu Endemik Sulawesi Apis nigrocincta (Smith, 1861) Hamsa, Hamsa; Masrianih, Masrianih; Trianto, Manap; Ashari, Abdul; Windarsih, Yulia; Nurdin, Musdalifah
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.15190

Abstract

The study aims to identify the main feed source for Apis nigrocincta through the analysis of polent morphology. The study uses explorative descriptive methods with qualitative and quantitative approaches. The focus of this study is the identification of feed source for endemic honeybees of Sulawesi, Apis nigrocincta through the analysis of polent morphology. Through the approach of polent morphology analysis, successfully identified 12 plant species from 9 families that could potentially be feed sources such as, Cucurbita maxima, Clematis viticella, Clematis montana, Pseuderanthemum tropurpureum, Cersicaria odorata, Solamun torvum, Solamun nigrum, Pine mercussii, Piper betle, Piper nigrum, and Galium aparine identification showed the presence of polyn diversity that supports the nutritional needs of bees, and emphasized the importance of local vegetation conservation to maintain the sustainability of the A. nigrocincta population. The results of the study showed that the polen has characteristics such as the polish unit, size, shape, shape on the P and E side, aperture type, as well as exin ornamentation. The polents collected by bees A. nigrocincta come from various types of plants, namely fruits, ornamental plants, vegetable plants, herb plants, and trees. This diversity reflects the flexibility of bees in choosing food sources based on availability in the surrounding environment. The results of the identification of the polymer samples showed that 12 species of plants that are the main feed source for A. nigrocincta bees are sourced from the polen. Each species has different flower characteristics, both in terms of shape and color. Polen diversity indicates that the environment around Jononunu Village has a rich ecosystem with different types of flowering plants.
Co-Authors Abd Rauf Abdul Hakim Laenggeng Achmad Ramadhan Achmad Ramadhan Adnan, Bahana Aditya Agung Dwi Kurniawan Ahmad Ramadhan Akram, Akram Alimudin, Sukmawati Amalia Buntu Amiruddin Kasim Anisa Anisa Aprilya, Dinda Ari Pratama Artha, Fandy Ashari, Abdul Asmara, Dudun Candra Asriani Hasanuddin Astija Bahana Aditya Adnan Baharuddin Hamzah Biung, Yelsan Kanan Burhanuddin, Alga Patricia Bustamin Bustamin Bustamin Bustamin Carolus Noprianto Carolus Noprianto Daratul Maulydiia Delima, Nur Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham, Dirham EFENDI Efendi Efendi Efendi Fajri Marisa Fajri Marisa Fajri Marisa Fajri Marisa Faried, Dirham Fariza, Ifat Fatmah Dhafir Febriawan, Aan Hamsa, Hamsa Hari Purwanto Hari Purwanto Hartina, Siti Hayyatun Mawaddah Herjayanti, Nur Herlangga Adiputra Hidyanisa, Dhea Hj. Musdalifah Nurdin I Ketut Suada I Made Budiarsa I Nengah Kundera Idham Cholik Ramadhan Isninar, Isnainar Ivan Permana Putra Kaini, Kaini Kaltisa, Kaltisa Kundera, I Negah Kusumaningati, Lilis Tri Laksono Trisnantoro Lembah, Rocky Reviko T. Lestari M.P Alibasyah M. Abimanyu Marisa, Fajri Masriani . Masrianih Moh Dahri Kisman Moh Dahri Kisman Moh Dahri Kisman Moh Dahri Kisman Moh. Dahri Kisman Mohammad Jamhari Mursito S. Bialangi Ni Gusti Ayu Galuh Candra Kirana Ni Putu Siswandari Ningsi, Ririn Apriani Noprianto, Carolus Novalina Serdiati Novi, Winda Cinthia NOVITASARI Nur Alam Nur Herjayanti Nur Herjayanti Nur Hikmah Nur'aini Nur'aini Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Trianto Nurfadila A, Nurfadila A Nurfadilah Rahma Nuryanti, Sitti Pratama, Rizaldy Pratama, Sandi Fransisco Puspitasari, Amira Putri, Regina Triana RAFIQA RAFIQA, RAFIQA Rahayu, Irma Septi Raya Agni Ritman Ishak Paudi Rizaldy Pratama Rocky Reviko T. Lembah Rocky RT Lembah Rosichon Ubaidillah Saliyem, Saliyem Samsurizal, M. Sulaeman Saridewi, Uut Sitti Nur Fadhilah Sri Hardianti sukmawati Sukmawati Sukmawati Sukmawati Sukmawati Sukmawati Suprianto Sutrisnawati Mardin Syech Zainal Tiktiek Kurniawati Trianto, Nuraini Tuty Arisuryanti Varsha Salsabillah Wahyudi Wahyudi Warsih, Eko Winarsih Winarsih Windarsih, Yulia Zasmita, Eka Zulaikhah Dwi Jayanti