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All Journal Buletin Peternakan METAMORFOSA Journal of Biological Sciences Biotropika Biology, Medicine, & Natural Product Chemistry Journal of Tropical Biodiversity and Biotechnology BIOMA : Jurnal Biologi Makassar Jurnal Biodjati BIOEDUSCIENCE Jurnal Biologi Tropis Justek : Jurnal Sains Dan Teknologi Biosel: Biologi Science and Education Jurnal Pengabdian Magister Pendidikan IPA Biota BIO-EDU: Jurnal Pendidikan Biologi Jurnal Abdidas Koordinat : Jurnal Pembelajaran Matematika dan Sains Bioscientist : Jurnal Ilmiah Biologi Media Eksakta AGRI-TEK : Jurnal Penelitian Ilmu-ilmu Eksakta Interdisciplinary International Journal of Conservation and Culture Journal of Biology Science and Education
Manap Trianto
Departement Of Biology Education, Faculty Of Teacher Training And Education, Tadulako University
Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Flavonoid and Alkaloid Content of Red Onion Skin Extract (Allium cepa L.) Windarsih, Yulia; Masrianih; Trianto, Manap
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8578

Abstract

Red onion skin (Allium cepa L.) is often discarded as waste, despite being rich in flavonoids and alkaloids, which possess antioxidant, anti-inflammatory, antimicrobial, and anticancer properties. This study aims to determine the flavonoid and alkaloid content of red onion skin extract using reflux and Soxhlet extraction methods with 96% ethanol as the solvent. The extraction was performed using reflux and Soxhlet techniques, followed by UV-Vis spectrophotometry to quantify flavonoid and alkaloid content. A quercetin standard curve was used to determine flavonoid levels, while a caffeine standard curve was applied for alkaloid content analysis. The Soxhlet method yielded a higher flavonoid content (108.21 mg/g or 10.82%) compared to the reflux method (105.55 mg/g or 10.55%). In contrast, the reflux method resulted in a higher alkaloid content (169.56 mg/g or 16.95%) than Soxhlet extraction (167.0 mg/g or 16.70%). These differences are attributed to the continuous solvent circulation in Soxhlet extraction, which enhances flavonoid solubility, while the lower temperature in the reflux method prevents alkaloid degradation. The findings suggest that Soxhlet extraction is more effective for flavonoid extraction, whereas reflux extraction is better for alkaloid extraction. Given their significant bioactive properties, red onion skin extracts have potential applications in the pharmaceutical, nutraceutical, and food industries. Future research should focus on optimizing extraction techniques and conducting toxicological and pharmacological studies to explore the therapeutic potential of red onion skin extract.
Molecular Identification of Lepidopteran Predators of Coccidae on Coffee Plants (Coffea canephora) in Sigi Regency Budiarsa, I Made; Trianto, Manap; Adnan, Bahana Aditya
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.983-992

Abstract

This study aimed to identify the species of Lepidopteran predators that prey on soft scale insects (Coccidae) found on coffee plants in Sigi Regency using molecular techniques. DNA was extracted using the modified CTAB method (Doyle & Doyle, 1990). PCR amplification targeted the COI gene using universal primers LCO1490 and HCO2198 with a GeneAmp PCR System 9700. Visualization was done using 1% agarose gel and UV-transilluminator. Sequencing was conducted externally. Data were analyzed using BioEdit 7.2.5, BLAST, BOLD Systems, and MEGA11 software. The DNA sequence of the predator sample showed 97.91% similarity to Autoba rubra based on GenBank and 97.59% in BOLD Systems. Phylogenetic analysis confirmed a close genetic relationship with A. rubra, distinct from Eublemma. Morphological similarities with Eublemma were misleading, highlighting the accuracy of molecular identification. This study is the first to confirm the identity of a Lepidopteran predator of coffee scale insects in Indonesia using molecular techniques, revealing its close relation to Autoba rubra. The results correct previous morphological misidentifications and contribute new data on predator diversity in biological control contexts.
Analisis Filogenetik Apis dorsata (Fabricius 1793) Bedasarkan Gen Chytochrome Oxidaxe I (COI) Delima, Nur; Masrianih, Masrianih; Trianto, Manap; Windarsih, Yulia; Buntu, Amalia; Dhafir, Fatmah
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14899

Abstract

Apis dorsata is a giant honeybee species distributed across South and Southeast Asia. Phylogenetic analysis is used to illustrate the evolutionary relationships of organisms through a phylogenetic tree. This study aims to describe the genetic relationships of A. dorsata based on the cytochrome oxidase I (COI) gene. Sampling was conducted using an exploratory method in various regions, followed by DNA isolation, DNA amplification, sequencing, and bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The results show that phylogenetic analysis using the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that clearly depicts evolutionary relationships. A. dorsata from Central Sulawesi is more closely related to other A. dorsata species within the same internal cluster, while other species are separated. These findings reveal a clear pattern of genetic relationships among A. dorsata populations.
Analisis Filogenetik Cryptic species Apis cerana Fabricius, 1793 Berdasarkan Gen 16S rRNA Puspitasari, Amira; Budiarsa, I Made; Ashari, Abdul; Dhafir, Fatmah; Windarsih, Yulia; Trianto, Manap
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14967

Abstract

Apis cerana is a member of the Insecta class grouped in the order Hymenoptera. Phylogenetic analysis is used to describe the kinship of living things through phylogeny trees. This study aims to describe the phylogenetic relationship of cryptic species in Apis cerana based on the 16S rRNA gene. Sampling use the cruising method in various regions, the DNA isolation, DNA amplification, sequencing, followed by bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The result showed that phylogenetic analysis with the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates clear evolutionary relationship. A. cerana from Central Sulawesi is more closely related to other A. cerana species in the same clade, while other species are separated. The result of this study show a clear pattern of kinship between A. cerana populations.
Analisis Filogenetik Lebah Madu Endemik Sulawesi Apis nigrocincta Smith, 1860 Berdasarkan Gen 16S rRNA Hidyanisa, Dhea; Budiarsa, I Made; Ashari, Abdul; Trianto, Manap; Nurdin, Musdalifah; Windarsih, Yulia
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14968

Abstract

Apis nigrocincta is an endemic honey bee that is only found in two regions in Indonesia, namely the Sangihe Islands and Sulawesi Islands. Phylogenetic analysis is used to describe the phylogenetic relationship of Sulawesi endemic honey bee A. nigrocincta based on the 16S rRNA gene. Sampling using the cruising method in various regions, then DNA isolation, DNA amplification, sequencing, and continued bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The results showed that phylogenetic analysis with the Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates clear evolutionary relationships. A. nigrocincta from Central Sulawesi is more closely related to other A. nigrocincta species in the same clade, while other species are separated. The results of this study show a clear pattern of kinship between A. nigrocincta populations. 
Analisis Filogenetik Lebah Tukang Kayu Xylocopa appendiculata Smith, 1852 Berdasarkan Gen Cytochrome Oxidase 1 (CO1) Rahayu, Irma Septi; Budiarsa, I Made; Ashari, Abdul; Trianto, Manap; Masrianih, Masrianih; Rafiqa, Rafiqa
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14969

Abstract

Xylocopa appendiculata  is an insect from the order Hymenotera and has bright yellow morphological characteristics on the thorax, while the rest of the body is black. Phylogenetic analysis is used to describe the kinship of living things throughh phylogeny trees. This study aims to describe the phylogenetic relationship of carpenter bees Xylocopa appendiculata based on the cytochrome oxidase 1 (CO1). DNA isolation, DNA amplification, sequencing, followed by bioinformatics analysis using GeneStudio, DNASTAR, MESQUITE, and MEGA 11. The results showed that phylogenetic analysis using Neighbor-Joining (NJ) and Maximum Likelihood (ML) methods produced a phylogenetic tree that illustrates a clear evalutionary relationship X. appendiculata from Central Sulawesi is more closely related to other X. appendiculata species in the same clade. The results of this study show a clear kinship pattern between populations of X. Appendiculata.
Identifikasi Pakan Lebah Madu Endemik Sulawesi Apis nigrocincta (Smith, 1861) Hamsa, Hamsa; Masrianih, Masrianih; Trianto, Manap; Ashari, Abdul; Windarsih, Yulia; Nurdin, Musdalifah
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.15190

Abstract

The study aims to identify the main feed source for Apis nigrocincta through the analysis of polent morphology. The study uses explorative descriptive methods with qualitative and quantitative approaches. The focus of this study is the identification of feed source for endemic honeybees of Sulawesi, Apis nigrocincta through the analysis of polent morphology. Through the approach of polent morphology analysis, successfully identified 12 plant species from 9 families that could potentially be feed sources such as, Cucurbita maxima, Clematis viticella, Clematis montana, Pseuderanthemum tropurpureum, Cersicaria odorata, Solamun torvum, Solamun nigrum, Pine mercussii, Piper betle, Piper nigrum, and Galium aparine identification showed the presence of polyn diversity that supports the nutritional needs of bees, and emphasized the importance of local vegetation conservation to maintain the sustainability of the A. nigrocincta population. The results of the study showed that the polen has characteristics such as the polish unit, size, shape, shape on the P and E side, aperture type, as well as exin ornamentation. The polents collected by bees A. nigrocincta come from various types of plants, namely fruits, ornamental plants, vegetable plants, herb plants, and trees. This diversity reflects the flexibility of bees in choosing food sources based on availability in the surrounding environment. The results of the identification of the polymer samples showed that 12 species of plants that are the main feed source for A. nigrocincta bees are sourced from the polen. Each species has different flower characteristics, both in terms of shape and color. Polen diversity indicates that the environment around Jononunu Village has a rich ecosystem with different types of flowering plants.
Identifikasi Jenis Tumbuhan Paku (Pteridophyta) di Jalur Pendakian Gunung Nokilalaki Desa Tongoa Kecamatan Palolo Kabupaten Sigi Asmara, Dudun Candra; Jamhari, Mohammad; Febriawan, Aan; Trianto, Manap; Astija, Astija; Nurdin, Musdalifah
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.15253

Abstract

The purpose of this study was to identify the types of ferns (Pteriodophyta) found on the Nokilalaki Mountain Climbing Trail, Tongoa Village, Palolo District, Sigi Regency. This type of research is a qualitative descriptive research using the exploration method and free collection sampling techniques. This study aims to describe existing phenomena. In it there are efforts to describe, record, and analyze the conditions that are happening factually. The results of the study conducted on the Mount Nokilalaki Climbing Trail, Tongoa Village, Palolo District, Sigi Regency obtained 26 types of ferns consisting of 4 classes, 6 orders, 14 families. Of the 26 types of ferns (Pteridophyta) found, namely Dryopteris filix-mas, Dryopteris marginalis, Rumohra adiantiformis, Neprolepis cordifolia, Athyrium macrocarpum, Asplenium nidus, Asplenium polyodon, Davallia trichomanoides, Microsorum fortune, Pteris biaurita, Nephrolepis biserrata, Nephrolepis sp, Microlepia speluncea, Microsorum pustulatum, Pyrrosia lanceolata, Pyrrosia philoselloides, Microsorum punctatum, Vittaria elongate, Angiopteris evecta, Cristella dentate, Selaginela wildewoii, Selaginela involvens, Selaginela sp, Selaginella apoda, Huperzia lucidula. Hymenophyllum sp.
Hubungan Kekerabatan Kupu- Kupu (Lepidoptera) Berdasarkan Karakter Morfologi di Desa Pombewe, Sulawesi Tengah Artha, Fandy; Dhafir, Fatmah; Ashari, Abdul; Trianto, Manap; Bustamin, Bustamin; Rafiqa, Rafiqa
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.15309

Abstract

This study aims to analyze the kinship of butterflies (Lepidoptera) based on morphological characters in Pombewe Village, Central Sulawesi. The research method used was the roaming method with free collection technique. Morphological characters observed included antenna shape, wing color and pattern, and body size. Kinship analysis was conducted using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) with the help of Paleontological Statistics software (PAST 4.03). The results identified 13 butterfly species from the Nymphalidae family, namely Euploea redtenbacheri, Euploea algea, Euploea hewitsonii, Danaus genutia leucoglene, Cyrestis strigata, Danaus genutia genutia, Euploea eunice, Vindula erota, Doleschallia polibete, Acraea terpsicore, Ideopsis juventa, Parantica cleona, and Cyrestis paulinus. The relationship dendrogram showed two main clusters with four relationship categories, namely very close relationship (Cyrestis strigata and Cyrestis paulinus, IS = 100%), close relationship (Euploea hewitsonii and Danaus genutia leucoglene, IS = 74.07%), not close relationship (Cyrestis strigata and Euploea eunice, IS = 32.25%).
Hubungan Kekerabatan Kepiting Biola (Uca spp.) di Desa Lalombi Kecamatan Banawa Selatan Kabupaten Donggala Berdasarkan Karakter Morfologi Biung, Yelsan Kanan; Budiarsa, I Made; Trianto, Manap; Kundera, I Nengah; Ashari, Abdul; Jayanti, Zulaikhah Dwi
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.16380

Abstract

This study aims to analyze the phylogenetic relationships among six species of fiddler crabs (Uca spp.) found in Lalombi Village, Banawa Selatan Subdistrict, Donggala Regency, based on morphological characters. A descriptive-exploratory approach was employed, with purposive sampling to select representative specimens. Eighteen morphometric characters were measured from three individuals of each species: U. triangularis, U. perplexa, U. dussumieri, U. annulipes, U. tetragonon, and U. bellator. Data were analyzed using Principal Component Analysis (PCA) and UPGMA clustering with the aid of PAST software version 4.03. The PCA results revealed that carapace length (CL), anterior carapace width (ACW), and posterior carapace width (PCW) contributed significantly to total morphological variation. Cluster analysis indicated a close phylogenetic relationship between U. tetragonon and U. triangularis, as well as between U. perplexa and U. bellator. U. annulipes appeared intermediate between the two groups, suggesting a moderate level of relatedness. In contrast, U. dussumieri formed a distinct branch, likely due to its markedly larger body size compared to the other species. These findings affirm that morphometric characters are valuable in elucidating phylogenetic relationships within the genus Uca and support taxonomy based on morphological traits.
Co-Authors Abd Rauf Abd. Rauf Abia, Hikma Achmad Ramadhan Achmad Ramadhan Adnan, Bahana Aditya Agung Dwi Kurniawan Ahmad Ramadhan Akram, Akram Alimudin, Sukmawati Amalia Buntu Amiruddin Kasim Anisa Anisa Aprilya, Dinda Ari Pratama Artha, Fandy Ashari, Abdul Asmara, Dudun Candra Astija Bahana Aditya Adnan Baharuddin Hamzah Biung, Yelsan Kanan Burhanuddin, Alga Patricia Bustamin Bustamin Bustamin Bustamin Bustamin Carolus Noprianto Carolus Noprianto Daratul Maulydiia Delima, Nur Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham Dirham, Dirham Efendi EFENDI Efendi Efendi Fajri Marisa Fajri Marisa Fajri Marisa Fajri Marisa Faried, Dirham Fariza, Ifat Fatma Dhafir Fatmah Dhafir Febriani, Vita Indri Febriawan, Aan Hamsa, Hamsa Hari Purwanto Hari Purwanto Hartina, Siti Hayyatun Mawaddah Herjayanti, Nur Herlangga Adiputra Hidyanisa, Dhea Hj. Musdalifah Nurdin I Ketut Suada I Made Budiarsa I Nengah Kundera Idham Cholik Ramadhan Isninar, Isnainar Ivan Permana Putra Kaini, Kaini Kaltisa, Kaltisa Khaerunnisa Kundera, I Negah Kusumaningati, Lilis Tri Laksono Trisnantoro Lembah, Rocky Reviko T. Lestari M.P Alibasyah M. Abimanyu Marisa, Fajri Masriani . Masrianih Moh Dahri Kisman Moh Dahri Kisman Moh Dahri Kisman Moh Dahri Kisman Moh. Dahri Kisman Mohammad Jamhari Mursito S. Bialangi Ni Gusti Ayu Galuh Candra Kirana Ni Putu Siswandari Ningsi, Ririn Apriani Noprianto, Carolus Novalina Serdiati Novi, Winda Cinthia NOVITASARI Nur Alam Nur Herjayanti Nur Herjayanti Nur Hikmah Nur'aini Nur'aini Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Nuraini Trianto Nurfadila A, Nurfadila A Nurfadilah Rahma Pratama, Rizaldy Pratama, Sandi Fransisco Puspitasari, Amira Putri, Regina Triana RAFIQA RAFIQA, RAFIQA Rahayu, Irma Septi Raya Agni Ritman Ishak Paudi Rizaldy Pratama Rocky Reviko T. Lembah Rocky RT Lembah Rosichon Ubaidillah Saliyem, Saliyem Samsurizal, M. Sulaeman Saridewi, Uut Sitti Nur Fadhilah Sri Hardianti Sukmawati sukmawati Sukmawati Sukmawati Sukmawati Sukmawati Suprianto Sutrisnawati Mardin Syech Zainal Tiktiek Kurniawati Trianto, Nuraini Tuty Arisuryanti Varsha Salsabillah Wahyudi Wahyudi Warsih, Eko Winarsih Winarsih Windarsih, Yulia Zasmita, Eka Zulaikhah Dwi Jayanti