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Afiff , Usamah
Divisi Mikrobiologi Medik, Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Library & Information Science Medicine & Pharmacology Public Health Veterinary

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Resistansi Escherichia coli asal feses sapi di wilayah Bogor terhadap antimikrob Rotinsulu, Dordia Anindita; Afiff, Usamah; Septiriyanti, Diyah
ARSHI Veterinary Letters Vol. 6 No. 4 (2022): ARSHI Veterinary Letters - November 2022
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.6.4.75-76

Abstract

Resistansi bakteri terhadap antimikrob telah menjadi permasalahan global. Pengujian resistansi antimikrob bakteri dari hewan penting dilakukan terutama dengan adanya resistansi antimikrob pada manusia yang diduga bersumber pada ternak. Tujuan penelitian ini adalah untuk menganalisis gambaran resistansi Escherichia coli (E. coli) yang diisolasi dari feses sapi terhadap berbagai antimikrob, yaitu aztreonam, basitrasin, sefpodoksim, enrofloksasin, fosfomisin, gentamisin, dan kloramfenikol. Uji resistansi antimikrob dilakukan menggunakan metode difusi cakram Kirby-Bauer. Hasil uji diinterpretasi menurut Clinical and Laboratory Standards Institute (CLSI). Isolat E. coli asal feses sapi yang diperiksa resistan terhadap aztreonam (40%), sefpodoksim (40%), fosfomisin (50%), basitrasin (100%), dan gentamisin (10%). Isolat E. coli sebanyak 30% memiliki kepekaan intermediat terhadap fosfomisin. Seluruh isolat sensitif terhadap kloramfenikol (100%), dan sebagian besar sensitif terhadap enrofloksasin (90%) dan gentamisin (90%). Isolat E. coli yang resistan terhadap aztreonam dan sefpodoksim diduga berfenotip extended spectrum β-lactamase (ESBL). Pengobatan infeksi E. coli pada sapi harus memperhatikan gambaran resistansi isolat terhadap antimikrob.
Prevalensi Penyakit oleh Parasit Darah Anjing di Klinik Hewan Sahabat, Melaka, Malaysia Ying, Megan Chan Zhi; Wijaya, Agus; Afiff, Usamah
ARSHI Veterinary Letters Vol. 7 No. 4 (2023): ARSHI Veterinary Letters - November 2023
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.7.4.71-72

Abstract

Penyakit yang disebabkan oleh parasit darah anjing (penyakit yang ditularkan melalui kutu), adalah suatu kondisi ketika anjing digigit oleh kutu yang membawa patogen. Tujuan dari penelitian ini adalah untuk menilai prevalensi penyakit yang disebabkan oleh parasit darah anjing (Anaplasmosis, Babesiosis, dan Ehrlichliosis) di Klinik Hewan Sahabat, Melaka, Malaysia dari Agustus 2020 hingga Juli 2022. Data sekunder dari 103 anjing yang terinfeksi diambil dari rekam medis. Anjing pada tahap anak anjing lebih sering didiagnosis yaitu sebesar 26,21%. Baik jantan maupun betina menunjukkan prevalensi yang hampir sama yaitu 52,43% dan 47,57%. Anjing kampung memiliki prevalensi yang relatif lebih tinggi dibandingkan dengan anjing ras, tetapi tidak ada kecenderungan ras. Kasus infeksi ganda lebih sering terjadi dibandingkan dengan kasus infeksi tunggal. Penyakit yang ditularkan melalui kutu anjing menunjukkan prevalensi yang lebih tinggi di antara musim pancaroba di Malaysia. Tanda-tanda klinis yang paling umum yang ditunjukkan oleh anjing adalah anoreksia, tidak aktif, dan diatesis pendarahan. Untuk mendukung diagnosa, anemia dan trombositopenia dapat diamati dari hasil hitung darah lengkap pasien, alat tes akan menunjukkan hasil positif ketika antibodi atau antigen terdeteksi dan parasit juga dapat diamati di bawah mikroskop dengan menggunakan apusan darah. Prevalensi penyakit yang ditularkan melalui kutu akan dipengaruhi oleh faktor intrinsik (usia, jenis kelamin) dan faktor ekstrinsik (cuaca).
Identification and antibiotic resistance patterns of Klebsiella sp. isolated from the feces of Bornean Orangutans (Pongo pygmaeus) at Taman Safari Indonesia, Bogor Purnamaningrum, Nora Dyah Ayu; Afiff, Usamah; Sunartatie, Titiek; Safika, Safika; Hidayat, Rahmat; Supratikno, Supratikno; Astuti, Yohana Tri
ARSHI Veterinary Letters Vol. 8 No. 2 (2024): ARSHI Veterinary Letters - May 2024
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.8.2.39-40

Abstract

Orangutan adalah satu-satunya kera besar yang masih bertahan di wilayah Asia Tenggara. Namun, populasi mereka di habitat liar terus menurun, dengan perkiraan menunjukkan penurunan sebesar 50% selama 60 tahun terakhir. Data mikrobiologis mengenai resistensi antibiotik Klebsiella sp. dalam feses Orangutan Kalimantan (Pongo pygmaeus) masih terbatas. Penelitian ini bertujuan untuk mengidentifikasi keberadaan Klebsiella sp. dan mengkaji pola resistensinya terhadap berbagai antibiotik dalam feses Orangutan Kalimantan. Penelitian ini melibatkan isolasi, identifikasi, dan pengujian resistensi antibiotik. Hasilnya menunjukkan bahwa tiga isolat (37,5%) positif untuk Klebsiella sp. Uji sensitivitas mengungkapkan dua pola resistensi yang berbeda di antara isolat Klebsiella sp. dari feses P. pygmaeus yang sehat di Taman Safari Indonesia, Bogor. Isolat-isolat ini menunjukkan resistensi terhadap antibiotik β-laktam tetapi tetap sensitif terhadap antibiotik golongan aminoglikosida, tetrasiklin, dan fluorokuinolon.
Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb.) on the Immune Response and Gene Expression of Nile Tilapia (Oreochromis niloticus) Infected with Aeromonas hydrophila Fauzi, Arini Resti; Yuhana, Munti; Widanarni, Widanarni; Setiawati, Mia; Afiff, Usamah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.44079

Abstract

Abstract Dayak onion (Eleutherine bulbosa (Mill.) Urb.) has the potential to be an immunostimulant to benefit fish health. This study aimed to evaluate the effectiveness of dietary supplementation with the powder and crude ethanol extracts of Dayak onion on the hematology and immune response parameters of Nile tilapia challenged with Aeromonas hydrophila. The research used a completely randomized design with six treatments and three replications. Nile tilapias were fed with Dayak onion (w/v) consisting of powders of 5% (P5), 10% (P10), and 15% (P15), while crude extract was 0.5% (E05) and given during rearing for 30 days. Positive control (C+) and negative control (C-) were used without Dayak onion. Nile tilapia in treatment C+, P5, P10, and P15 were injected with A. hydrophila 10⁶ CFU mL⁻1, while that in treatment C- was injected with phosphate buffered saline (PBS) intramuscularly. The results showed that the dietary with 15% powder and 0.5% crude extract significantly improved the hematology and immune response parameters compared to the control after the challenge against A. hydrophila (P<0.05). Dayak onion supplementation effectively increased the health status based on hematology and immune response parameters of Nile tilapia against A. hydrophila infection. Highlight Research Dayak onion has significant potential to improve nonspecific immunity in fish. Dayak onion improves hematology and immune response of Nile tilapia. Dayak onion can significantly regulate the immune system against A. hydrophila infection. Dayak onion significantly increases the production of the gene expression of IL-1β and TNF-α.
EFFECTIVENESS OF BIOFLOC, PROBIOTICS AND THE COMBINATIONS ON GROWTH, IMMUNE RESPONSES AND RESISTANCE OF VANNAMEI SHRIMP INFECTED WITH Vibrio parahaemolyticus Wicaksono, Baref Agung; Widanarni, Widanarni; Yuhana, Munti; Gustilatov, Muhamad; Afiff, Usamah
Indonesian Aquaculture Journal Vol 20, No 1 (2025): (June, 2025)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.20.1.2025.59-73

Abstract

Vibrio parahaemolyticus strain that produces PirA and PirB toxins is the main causative agent of Acute Hepatopancreatic Necrosis Disease (AHPND) in vannamei shrimp. This study aimed to evaluate the effect of biofloc application, probiotic Pseudoalteromonas piscicida 1Ub, and their combination on growth, immune response and resistance of vannamei shrimp infected with V. parahaemolyticus strain. This study used a completely randomized design consisting of biofloc-based system treatment with or without probiotic 1Ub and normal seawater as control. All treatment groups were challenged with V. parahaemolyticus AHPND strain at a cell density of 105 CFU mL”1 through immersion, while the negative control was reared without being pathogenic challenged. The shrimp used were in averaged body weight of 1.3 ± 0.002 g, reared for 21 days and fed five times a day at 06:00, 10:00, 14:00, 18:00, and 22:00 WIB. The results showed that the B+Pro combination challenge test treatment resulted the best growth performance (specific growth rate, absolute length gain and feed conversion ratio) (P<0.05) compared to other challenge test treatments. hrimp treated with B+Pro also showed a lower intestinal cell population of V. parahaemolyticus RfR, and significantly higher immune response values (P<0.05) than those of other challenge test treatments and K+. Furthermore, those parameters supported positive impact on final shrimp survival rates in the experiment. This study shows that the application of combination of biofloc and 1Ub probiotic bacteria can significantly protect and increase the resistance of vannamei shrimp to V. parahaemolyticus AHPND infection.
Co-Authors Abdul Samik Abdul Zahid Agus Wijaya Agus Wijaya Agustin Indrawati Alla Asmara Anggia Murni Wijiati Annisa Madyanti Geminastiti Parampasi Anriansyah Renggaman Ardilasunu Wicaksono Arini Resti Fauzi Asah Hilaliah Astuti, Yohana Tri Aulia Andi Mustika Beginer Subhan Chairani Ridha Maghfira Darna Andrian Ramadhan Dea Indriani Astuti Dedi Rahmat Setiadi Deni Noviana Denny Widaya Lukman Denny Widya Hikman Destri Prameswari, Alvira Dinamella Wahjuningrum Edi Sukmawinata Edi Sukmawinata Ekowati Handharyani Etih Sudarnika Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Faoziyatunnisa, Nurul Fauzi, Arini Resti Gustilatov, Muhamad Hardianti, Aprilia Hary Krettiawan Islami, Nurfara Ismahyuningsih, Revita Julie Ekasari Karen Lee koekoeh santoso Leng , Ang Jia Maria Luisa MNB Klobongona Men, Lydia Pow Kar Merlia Andriyani Mia Setiawati Michella Hoseana Wijaya MUHAMMAD AGIL Muhammad Arif Mulya MUNTI YUHANA Nabila Swarna Puspa Hermana Nauval Firdana, Chorrysa Nor Jannah, Nor Nugraha, Arifin Budiman Nuri Kamaliah, Syarifah Nursa Rima Putri Purnamaningrum, Nora Dyah Ayu Rahmat Hidayat Ridi Arif Risa Tiuria Risqika Akla Velayati Rizal Dwinto Rochman Ronald Tarigan Rotinsulu, Dordia Anindita Safika S, Safika Septiriyanti, Diyah sri murtini . Sri Nuryati Srihadi Agungpriyono Sudrajat, R Herman Suhendi, Adnan Rizal Sukenda . Suleman, Gabriella Augustine Supratikno, Supratikno Surya Agus Prihatno Tambun, Andreas Titiek Sunartatie Trioso Purnawarman Tsani Untsa, Agista Ulandari, Rafika Ulfatin Khoiriyah Herowati Vindriati, Zukhrufa Vista Wicaksono, Baref Agung WIDANARNI WIDANARNI Xin, Leaw Yi Ying, Megan Chan Zhi Yosua Kristian Adi Yusuf Ridwan