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Afiff , Usamah
Divisi Mikrobiologi Medik, Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Library & Information Science Medicine & Pharmacology Public Health Veterinary

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Dietary supplementation of Bacillus sp. NP5 and dayak onion simplicia powder Eleutherine bulbosa (Mill.) Urb. for the prevention of Aeromonas hydrophila in catfish Clarias sp. Sudrajat, R Herman; Yuhana, Munti; Widanarni, Widanarni; Julie Ekasari; Afiff, Usamah
Jurnal Akuakultur Indonesia Vol. 22 No. 2 (2023): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.22.2.134-146

Abstract

Aeromonas hydrophila is the main causative agent of ulcerative disease in catfish and causes considerable economic losses to Indonesian aquaculture. This study evaluates the prebiotic activity and the effect of feed supplementation of dayak onion simplicia powder (DOSP) on the immune response and survival of catfish infected with A. hydrophila. Five doses (0.20, 0.25, 0.30, 0.35, and 0.40 g/mL) of DOSP were tested in vitro to assess the prebiotic activity score. The results showed that a dose of 0.20 g/mL gave a significantly (P<0.05) higher probiotic stimulation value than other doses. In the in vivo test, the study used a completely randomized design with five treatments, namely simplicia (DOSP 20 g/kg), probiotic (PRO, Bacillus sp. NP5 108 CFU/mL, 1% v/w), combination (PRO+DOSP), and control (positive and negative). Fish were reared for 45 days and fed three times a day. On day 46, fish from all treatments, except negative control, were infected with an A. hydrophila dose of 106 CFU/mL injected intramuscularly. The results showed that the combination treatment (PRO+DOSP) gave better total erythrocytes, hemoglobin, hematocrit, total leukocytes and phagocytosis activity than probiotics, DOSP, and control. Administering the combination (PRO+DOSP) can reduce the total number of A. hydrophila lower than the probiotic, DOSP, and control treatments. In addition, the survival rate of catfish in the combined treatment (PRO+DOSP) was significantly (P<0.05) higher than probiotics, DOSP, and control. The results of this study can be a helpful reference and application for the early prevention of A. hydrophila infection. Keywords: aquaculture, Bacillus sp. NP5, dayak onion, probiotics, simplicia powder ABSTRAK Aeromonas hydrophila adalah penyebab utama penyakit bercak merah pada budidaya ikan lele dan menyebabkan kerugian ekonomi cukup besar pada akuakultur Indonesia. Penelitian ini bertujuan untuk mengevaluasi aktivitas prebiotik serbuk simplisia bawang dayak (SSBD) dan pengaruh penambahan kombinasi probiotik Bacillus sp. NP5 dan SSBD pada pakan terhadap respons imun dan kelangsungan hidup ikan lele yang diinfeksi A. hydrophila. Lima dosis (0,20, 0,25, 0,30, 0,35, dan 0,40 g/mL) SSBD diuji secara in vitro untuk menilai skor aktivitas prebiotik. Hasil penelitian menunjukkan bahwa dosis 0,20 g/mL memberikan nilai stimulasi probiotik signifikan (P<0,05) lebih tinggi dibandingkan dosis lainnya. Pada uji in vivo, penelitian menggunakan rancangan acak lengkap terdiri dari lima perlakuan, yaitu simplisia (SSBD, serbuk simplisia bawang dayak 20 g/kg), probiotik (PRO, Bacillus sp. NP5 108 CFU/mL, 1% (v/w), kombinasi (PRO+SSBD), dan kontrol (positif dan negatif). Ikan dipelihara selama 45 hari dan diberi pakan tiga kali sehari. Pada hari ke 46, ikan pada semua perlakuan, kecuali kontrol negatif, di infeksi A. hydrophila dosis 106 CFU/mL secara intramuskular. Hasil penelitian menunjukkan bahwa kombinasi (PRO+SSBD) memberikan total eritrosit, hemoglobin, hematokrit, total leukosit dan aktivitas fagositosis lebih baik dibandingkan probitoik, SSBD, dan kontrol. Kombinasi (PRO+SSBD) mampu menekan total A. hydrophila lebih rendah dibandingkan probiotik, SSBD, dan kontrol. Selain itu, tingkat kelangsungan hidup ikan lele pada perlakuan kombinasi (PRO+SSBD) signifikan (P<0,05) lebih tinggi dibandingkan probitoik, SSBD, dan kontrol. Hasil penelitian ini bisa menjadi referensi dan aplikasi yang efektif untuk pencegahan dini infeksi A. hydrophila. Kata kunci: akuakultur, Bacillus sp. NP5, bawang dayak, probiotik, serbuk simplisia
Evaluation of single and multispecies probiotic applications for the prevention of Motile Aeromonads Septicaemia in gourami, Osphronemus gourami Tsani Untsa, Agista; Widanarni, Widanarni; Sukenda, Sukenda; Afiff, Usamah; Yuhana, Munti
Jurnal Akuakultur Indonesia Vol. 23 No. 1 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.1.56-70

Abstract

The aim of this study was to evaluate single and multispecies probiotic applications for the prevention of Motile Aeromonads Septicaemia (MAS) disease in gourami, Osphronemus gourami. The experiment consisted of the in vitro inhibition test and the in vivo application of probiotics in gourami. The in vivo assay, consisted of five treatments and five replicates, namely: negative control (K-); positive control (K+); (B) fish fed with supplementation of 1% (v/w) probiotic Bacillus NP5 RifR cells and challenged with Aeromonas hydrophila RifR; (L) fish fed with supplemention of probiotic Lactobacillus plantarum CipR 1% (v/w); challenged with A. hydrophila RifR; (BL) fish fed with mixture supplementation of Bacillus NP5 RifR 0.5% (v/w)+L. plantarum CipR 0.5%; infected with A. hydrophila RifR. Fish (29.57 ± 1.00 g) were reared in a 45 L volume aquaria with a rearing density of 10 fish/m3 for 50 days, with feeding trial three times a day. The challenge test was conducted by intramuscular injection with pathogenic A. hydrophila RifR cells (106 CFU/mL) on day 41st. In vitro test results showed that single and multispecies probiotics significantly (P<0.05) inhibited A.hydrophila RifR. In vivo experiment showed that probiotic supplementation treatments improved the growth performance, and microbiota diversity in the gut. The immune responses, fish resistance to A. hydrophila RifR and gourami survival rate in all treatments of supplemented feed were significantly higher compared to the positive control. The best treatment, multispecies probiotics significantly (P<0.05) improved the survival of gourami 96.67% post infection with A. hydrophila RifR. Keywords: A. hydrophila, Bacillus NP5, L. plantarum, O. gourami, probiotic ABSTRAK Penelitian ini bertujuan untuk mengevaluasi aplikasi probiotik tunggal dan multispesies dalam pencegahan penyakit Motile Aeromonad Septicaemia (MAS) yang disebabkan oleh Aeromonas hydrophila pada ikan gurami (Osphronemus gourami). Penelitian terdiri atas uji penghambatan in vitro bakteri probiotik tunggal dan multispesies terhadap A. hydrophila RifR, dan uji in vivo aplikasi probiotik untuk gurami. Uji in vivo, terdiri dari lima perlakuan dan lima ulangan, yaitu: kontrol negatif (K-) kontrol positif (K+); (B) ikan dengan pakan suplementasi Bacillus NP5 RifR 1% dan diinfeksi A. hydrophila RifR; (L) pakan komersial dengan penambahan probiotik L. plantarum CipR 1% dan infeksi A. hydrophila RifR; (BL) ikan dengan suplementasi campuran Bacillus NP5 RifR 0.5% dan L. plantarum CipR 0.5% serta diinfeksi A. hydrophila RifR. Benih gurami (29.57 ± 1.00 g) dipelihara di dalam akuarium bervolume 45 L dengan kepadatan 10 ekor/m3 selama 50 hari, dengan pakan bersuplemen probiotik 3 kali sehari. Uji tantang dilakukan dengan menginjeksikan suspensi sel patogen A. hydrophila RifR (106 CFU/mL) secara intramuscular pada hari ke 41. Hasil uji in vitro menunjukkan bahwa probiotik tunggal dan multispesies dapat menghambat pertumbuhan sel A.hydrophila RifR di organ hati dan ginjal secara signifikan (P<0.05). Hasil uji in vivo menunjukkan perlakuan aplikasi probiotik tunggal dan multispesies mampu meningkatkan kinerja pertumbuhan, keragaman mikrobiota usus. Respons imunitas, resistansi terhadap A. hydrophila RifR dan kelangsungan hidup gurami di semua perlakuan pakan bersuplemen probiotik secara signifikan lebih tinggi dibandingkan kontrol positif. Perlakuan terbaik adalah probiotik multispesies meningkatkan kelangsungan hidup ikan sebesar 96.67% secara signifikan (P<0.05) pascainfeksi A. hydrophila RifR. Kata kunci: A. hydrophila, Bacillus sp. NP5, L. plantarum, O. goramy, probiotik
Application of probiotic microcapsules Bacillus cereus BR2 with different doses for the prevention of Aeromonas hydrophila NFC1 infection in catfish Clarias sp. Faoziyatunnisa, Nurul; Yuhana, Munti; Wahjuningrum, Dinamella; Nuryati, Sri; Afiff, Usamah
Jurnal Akuakultur Indonesia Vol. 23 No. 2 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.2.134-146

Abstract

This study aims to analyze the administration of microencapsulated probiotic Bacillus cereus BR2 through feed atdifferent doses on the survival, immune response, and digestive enzyme activity of catfish Clarias sp. infected with Aeromonas hydrophila NFC1. The research design consisted of five treatments and three replications: (K-) feeding without the supplementation of probiotics fish injected with Phosphate-buffered saline solution; (K+) feeding without the supplementation of probiotics, fish injected with A. hydrophila NFC1 cell suspension (106 CFU/mL); (P1) feeding with supplementation of B. cereus BR2 microcapsules at a dose of 0.5% (w/w); (P2) feeding with the supplementation of B. cereus BR2 microcapsules at a dose of 1% (w/w; (P3) feeding with the supplementation of B. cereus BR2 microcapsules at a dose of 2% (w/w). All treatments of P1, P2, and P3 were intramuscularly injected with A. hydrophila NFC1 cell suspension. Catfish in average body weight of 3.73 ± 0.22 g were reared in tanks filled with 30 liters of water, at a density of 15 fish per container. A feed supplementation trial was performed for 40 days with feeding times three times a day; and a 5% feeding rate reevaluated based on biomass. Fish rearing was continued after the challenge test with A. hydrophila NFC1 for 10 days. The results showed that the application of supplemented feed containing B. cereus BR2 probiotic microcapsules increased the survival rate, immune response, and digestive enzyme activities of catfish which was infected with A. hydrophila NFC1, 2% microcapsule supplementation demonstrated the best result. Keywords: Aeromonas hydrophila NFC1, Bacillus cereus BR2, catfish, microcapsule, probiotics ABSTRAK Penelitian ini bertujuan untuk menganalisis pemberian mikrokapsul probiotik Bacillus cereus BR2 melalui pakan dengan dosis berbeda terhadap sintasan, respons imun, dan aktivitas enzim pencernaan ikan lele Clarias sp. yang diinfeksi Aeromonas hydrophila NFC1. Rancangan penelitian yang digunakan terdiri dari lima perlakuan dan tiga ulangan, yaitu (K-) pemberian pakan tanpa suplementasi probiotik dan diinjeksi PBS; (K+) pemberian pakan tanpa suplementasi probiotik dan diinfeksi A. hydrophila NFC1; (P1) pemberian pakan dengan suplementasi mikrokapsul B. cereus BR2 dosis 0,5% (b/b); (P2) pemberian pakan dengan suplementasi mikrokapsul B. cereus BR2 dosis 1%; (P3) pemberian pakan dengan suplementasi mikrokapsul B. cereus BR2 dosis 2%, masing-masing diinfeksi A. hydrophila NFC1. Benih lele dengan ukuran bobot rata-rata sekitar 3.73 ± 0.22 g dipelihara di dalam akuarium bervolume 30 L dengan kepadatan 15 ekor per wadah selama 40 hari, dengan pemberian pakan bersuplemen sebanyak tiga kali sehari, dengan 5% pemberian pakan yang dievaluasi berdasarkan bobot biomassa. Pemeliharaan ikan dilanjutkan setelah uji tantang dengan A. hydrophila NFC1 (106 CFU/mL) yang dilakukan hingga 10 hari pasca injeksi. Hasil penelitian menunjukkan aplikasi mikrokapsul probiotik B. cereus BR2 melalui pakan mampu meningkatkan sintasan, respons imun, dan aktivitas enzim pencernaan ikan lele yang diinfeksi A. hydrophila NFC1, dengan suplementasi mikrokapsul 2% sebagai hasil terbaik. Kata kunci: Aeromonas hydrophila NFC1, Bacillus cereus BR2, ikan lele, mikrokapsul, probiotik
Growth, immune responses, and resistance of vannamei shrimp fed with Lactobacillus paracasei probiotic and paraprobiotic and infected with Vibrio parahaemolyticus Nuri Kamaliah, Syarifah; Yuhana, Munti; Widanarni, Widanarni; Setiawati, Mia; Afiff, Usamah
Jurnal Akuakultur Indonesia Vol. 24 No. 2 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.2.199-210

Abstract

Vannamei shrimp is one of the most economically valuable aquaculture commodities in Indonesia. One of the pathogenic bacteria that is often found in vannamei shrimp farming is Vibrio parahaemolyticus. This study aimed to analyze the effectivity of Lactobacillus paracasei probiotics and paraprobiotics through feed with different cell densities on growth, and immune responses infected with V. parahaemolyticus. Vannamei shrimp of size 0.63 ± 0.01 were reared in containers with a stocking density of 15 shrimp per container and supplemented feed for 30 days. The research design consisted of six treatments, each with three replicates, namely (K-) feeding without supplements and injected with phosphate-buffered saline (PBS), (K+) feeding without supplements and infected with V. parahaemolyticus (104 CFU/mL), (PRI) feeding with 1% (v/w) probiotic L. paracasei with cell density of 106 CFU/mL, (PRII) 1% (v/w) probiotic L. paracasei cell density 109 CFU/mL, (PAI) 1% (v/w) paraprobiotic L. paracasei cell density 106 CFU/mL, (PAII) 1% (v/w) paraprobiotic L. paracasei cell density 109 CFU/mL. All treatments, except K-, were infected with V. parahaemolyticus (104 CFU/mL). Vannamei shrimp rearing was continued post the challenge test with V. parahaemolyticus which was conducted up to six days post injection. The results showed that feeding both probiotic and paraprobiotic L. paracasei through feed has improved growth, immune response, protein fat retention, and digestive enzyme activity of vannamei shrimp better than those of control. As the recommendation for the disease control of V. parahaemolyticus is feed supplementation with 1% (v/w) probiotic L. paracasei with cell density of 109 CFU/mL. Keywords: Lactobacillus paracasei, paraprobiotic, probiotic, vannamei shrimp, Vibrio parahaemolyticus ABSTRAK Udang vaname merupakan salah satu komoditas akuakultur yang bernilai ekonomis tinggi di Indonesia. Salah satu bakteri patogen yang sering ditemukan dalam budidaya udang vaname ialah bakteri Vibrio parahaemolyticus. Penelitian ini bertujuan untuk menganalisis pemberian probiotik dan paraprobiotik Lactobacillus paracasei melalui pakan dengan kepadatan sel berbeda terhadap pertumbuhan, dan respons imunitas yang diinfeksi V. parahaemolyticus. Udang vaname dengan ukuran 0.63 ± 0.01 gr dipelihara di dalam kontainer dengan padat tebar 15 ekor per wadah dan pemberian pakan bersuplemen selama 30 hari. Rancangan penelitian terdiri dari enam perlakuan, tiga ulangan, yaitu (K-) pemberian pakan tanpa suplemen dan diinjeksi PBS, (K+) pemberian pakan tanpa suplemen dan diinfeksi V. parahaemolyticus (104 CFU/mL), (PRI) pemberian pakan dengan probiotik L. paracasei kepadatan sel 106 CFU/mL dosis 1% (v/w), (PRII) probiotik L. paracasei kepadatan sel 109 CFU/mL dosis 1% (v/w), (PAI) paraprobiotik L. paracasei kepadatan sel 106 CFU/mL dosis 1% (v/w), (PAII) paraprobiotik L. paracasei kepadatan sel 109 CFU/mL dosis 1% (v/w), dan masing-masing diinfeksi V. parahaemolyticus (104 CFU/mL). Pemeliharaan udang vaname dilanjutkan setelah uji tantang dengan V. parahaemolyticus yang dilakukan hingga enam hari pasca injeksi. Hasil penelitian menunjukkan bahwa pemberian probiotik maupun paraprobiotik L. paracasei melalui pakan telah meningkatkan pertumbuhan, respons imun, retensi lemak protein, dan aktivitas enzim pencernaan udang vaname lebih baik dibandingkan kontrol. Untuk pengendalian V. parahaemolyticus diperoleh hasil terbaik dengan aplikasi probiotik L. paracasei dosis 1% (v/w) dengan kepadatan sel 109 CFU/mL. Kata Kunci: Lactobacillus paracasei, paraprobiotik, probiotik, udang vaname, Vibrio parahaemolyticus
Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb.) on the Immune Response and Gene Expression of Nile Tilapia (Oreochromis niloticus) Infected with Aeromonas hydrophila Fauzi, Arini Resti; Yuhana, Munti; Widanarni, Widanarni; Setiawati, Mia; Afiff, Usamah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.44079

Abstract

Abstract Dayak onion (Eleutherine bulbosa (Mill.) Urb.) has the potential to be an immunostimulant to benefit fish health. This study aimed to evaluate the effectiveness of dietary supplementation with the powder and crude ethanol extracts of Dayak onion on the hematology and immune response parameters of Nile tilapia challenged with Aeromonas hydrophila. The research used a completely randomized design with six treatments and three replications. Nile tilapias were fed with Dayak onion (w/v) consisting of powders of 5% (P5), 10% (P10), and 15% (P15), while crude extract was 0.5% (E05) and given during rearing for 30 days. Positive control (C+) and negative control (C-) were used without Dayak onion. Nile tilapia in treatment C+, P5, P10, and P15 were injected with A. hydrophila 10⁶ CFU mL⁻1, while that in treatment C- was injected with phosphate buffered saline (PBS) intramuscularly. The results showed that the dietary with 15% powder and 0.5% crude extract significantly improved the hematology and immune response parameters compared to the control after the challenge against A. hydrophila (P<0.05). Dayak onion supplementation effectively increased the health status based on hematology and immune response parameters of Nile tilapia against A. hydrophila infection. Highlight Research Dayak onion has significant potential to improve nonspecific immunity in fish. Dayak onion improves hematology and immune response of Nile tilapia. Dayak onion can significantly regulate the immune system against A. hydrophila infection. Dayak onion significantly increases the production of the gene expression of IL-1β and TNF-α.
EFFECTIVENESS OF BIOFLOC, PROBIOTICS AND THE COMBINATIONS ON GROWTH, IMMUNE RESPONSES AND RESISTANCE OF VANNAMEI SHRIMP INFECTED WITH Vibrio parahaemolyticus Wicaksono, Baref Agung; Widanarni, Widanarni; Yuhana, Munti; Gustilatov, Muhamad; Afiff, Usamah
Indonesian Aquaculture Journal Vol 20, No 1 (2025): (June, 2025)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.20.1.2025.59-73

Abstract

Vibrio parahaemolyticus strain that produces PirA and PirB toxins is the main causative agent of Acute Hepatopancreatic Necrosis Disease (AHPND) in vannamei shrimp. This study aimed to evaluate the effect of biofloc application, probiotic Pseudoalteromonas piscicida 1Ub, and their combination on growth, immune response and resistance of vannamei shrimp infected with V. parahaemolyticus strain. This study used a completely randomized design consisting of biofloc-based system treatment with or without probiotic 1Ub and normal seawater as control. All treatment groups were challenged with V. parahaemolyticus AHPND strain at a cell density of 105 CFU mL”1 through immersion, while the negative control was reared without being pathogenic challenged. The shrimp used were in averaged body weight of 1.3 ± 0.002 g, reared for 21 days and fed five times a day at 06:00, 10:00, 14:00, 18:00, and 22:00 WIB. The results showed that the B+Pro combination challenge test treatment resulted the best growth performance (specific growth rate, absolute length gain and feed conversion ratio) (P<0.05) compared to other challenge test treatments. hrimp treated with B+Pro also showed a lower intestinal cell population of V. parahaemolyticus RfR, and significantly higher immune response values (P<0.05) than those of other challenge test treatments and K+. Furthermore, those parameters supported positive impact on final shrimp survival rates in the experiment. This study shows that the application of combination of biofloc and 1Ub probiotic bacteria can significantly protect and increase the resistance of vannamei shrimp to V. parahaemolyticus AHPND infection.
Antimicrobial susceptibility of coagulase-negative staphylococci isolated from red-tailed racers (Gonyosoma oxycephalum) Lydia Pow Kar Men; Usamah Afiff; Deni Noviana
ARSHI Veterinary Letters Vol. 6 No. 4 (2022): ARSHI Veterinary Letters - November 2022
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.6.4.71-72

Abstract

Antibiotic resistant coagulase-negative staphylococci (CoNS) have been reported around the world. The aim of this study was to determine the antimicrobial susceptibility of CoNS isolated from red–tailed racers. Samples were swabbed from the oral cavity of 5 wild caught red–tailed racers, and were identified with biochemical test using the Kirby–Bauer disc diffusion test interpreted by referring to Clinical and Laboratory Standards Institute. The results obtained 4 species of CoNS isolated from swab samples including S. sciuri, S. xylosus, S. lentus, and S. kloosii. The antibiotic resistance test of S. xylosus, S. sciuri, and S. lentus showed susceptibility to amoxicillin, gentamicin, erythromycin, bacitracin, vancomycin and oxacillin, but resistance towards penicillin G. S. sciuri isolated from snake number 1 was intermediate towards erythromycin. S. kloosii showed susceptibility towards amoxicillin, gentamicin, bacitracin, penicillin G, vancomycin, and oxacillin, but was resistant towards erythromycin
Resistansi Escherichia coli asal feses sapi di wilayah Bogor terhadap antimikrob Dordia Anindita Rotinsulu; Usamah Afiff; Diyah Septiriyanti
ARSHI Veterinary Letters Vol. 6 No. 4 (2022): ARSHI Veterinary Letters - November 2022
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.6.4.75-76

Abstract

Resistansi bakteri terhadap antimikrob telah menjadi permasalahan global. Pengujian resistansi antimikrob bakteri dari hewan penting dilakukan terutama dengan adanya resistansi antimikrob pada manusia yang diduga bersumber pada ternak. Tujuan penelitian ini adalah untuk menganalisis gambaran resistansi Escherichia coli (E. coli) yang diisolasi dari feses sapi terhadap berbagai antimikrob, yaitu aztreonam, basitrasin, sefpodoksim, enrofloksasin, fosfomisin, gentamisin, dan kloramfenikol. Uji resistansi antimikrob dilakukan menggunakan metode difusi cakram Kirby-Bauer. Hasil uji diinterpretasi menurut Clinical and Laboratory Standards Institute (CLSI). Isolat E. coli asal feses sapi yang diperiksa resistan terhadap aztreonam (40%), sefpodoksim (40%), fosfomisin (50%), basitrasin (100%), dan gentamisin (10%). Isolat E. coli sebanyak 30% memiliki kepekaan intermediat terhadap fosfomisin. Seluruh isolat sensitif terhadap kloramfenikol (100%), dan sebagian besar sensitif terhadap enrofloksasin (90%) dan gentamisin (90%). Isolat E. coli yang resistan terhadap aztreonam dan sefpodoksim diduga berfenotip extended spectrum β-lactamase (ESBL). Pengobatan infeksi E. coli pada sapi harus memperhatikan gambaran resistansi isolat terhadap antimikrob.
Resistensi Escherichia coli dari ulas kloaka burung hantu pemangsa ikan (Ketupa ketupu) terhadap beberapa antibiotik Revita Ismahyuningsih; Usamah Afiff; Srihadi Agungpriyono
ARSHI Veterinary Letters Vol. 7 No. 3 (2023): ARSHI Veterinary Letters - August 2023
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.7.3.59-60

Abstract

Resistensi Escherichia coli terhadap antibiotik telah menjadi permasalahan dalam dunia kesehatan, meskipun E. coli berperan sebagai flora normal, akan tetapi ada beberapa strain bersifat patogen. Tujuan dari penelitian ini adalah untuk mengetahui pola resistensi isolat E. coli dari swab kloaka buffy fish owl (Ketupa ketupu) dari penangkaran burung terhadap streptomisin, tetrasiklin, gentamisin, asam nalidiksat, ampisilin, dan doksisiklin dengan uji difusi cakram Kirby-Bauer. Zona hambat yang terbentuk, dibandingkan dengan Clinical Laboratory Standards Institute (CLSI) 2020. Hasil penelitian menunjukkan bahwa semua isolat telah mengalami resistensi terhadap antibiotik ampisilin, namun masih sensitif terhadap gentamisin. Dua isolat masih sensitif terhadap tetrasiklin, asam nalidiksat, doksisiklin, dan streptomisin. Satu isolat menunjukkan intermediet terhadap streptomisin dan menunjukkan resistensi terhadap tetrasiklin, asam nalidiksat dan doksisiklin.
Prevalensi Penyakit oleh Parasit Darah Anjing di Klinik Hewan Sahabat, Melaka, Malaysia Megan Chan Zhi Ying; Agus Wijaya; Usamah Afiff
ARSHI Veterinary Letters Vol. 7 No. 4 (2023): ARSHI Veterinary Letters - November 2023
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.7.4.71-72

Abstract

Penyakit yang disebabkan oleh parasit darah anjing (penyakit yang ditularkan melalui kutu), adalah suatu kondisi ketika anjing digigit oleh kutu yang membawa patogen. Tujuan dari penelitian ini adalah untuk menilai prevalensi penyakit yang disebabkan oleh parasit darah anjing (Anaplasmosis, Babesiosis, dan Ehrlichliosis) di Klinik Hewan Sahabat, Melaka, Malaysia dari Agustus 2020 hingga Juli 2022. Data sekunder dari 103 anjing yang terinfeksi diambil dari rekam medis. Anjing pada tahap anak anjing lebih sering didiagnosis yaitu sebesar 26,21%. Baik jantan maupun betina menunjukkan prevalensi yang hampir sama yaitu 52,43% dan 47,57%. Anjing kampung memiliki prevalensi yang relatif lebih tinggi dibandingkan dengan anjing ras, tetapi tidak ada kecenderungan ras. Kasus infeksi ganda lebih sering terjadi dibandingkan dengan kasus infeksi tunggal. Penyakit yang ditularkan melalui kutu anjing menunjukkan prevalensi yang lebih tinggi di antara musim pancaroba di Malaysia. Tanda-tanda klinis yang paling umum yang ditunjukkan oleh anjing adalah anoreksia, tidak aktif, dan diatesis pendarahan. Untuk mendukung diagnosa, anemia dan trombositopenia dapat diamati dari hasil hitung darah lengkap pasien, alat tes akan menunjukkan hasil positif ketika antibodi atau antigen terdeteksi dan parasit juga dapat diamati di bawah mikroskop dengan menggunakan apusan darah. Prevalensi penyakit yang ditularkan melalui kutu akan dipengaruhi oleh faktor intrinsik (usia, jenis kelamin) dan faktor ekstrinsik (cuaca).
Co-Authors Abdul Samik Abdul Zahid Agus Wijaya Agus Wijaya Agustin Indrawati Alla Asmara Ang Jia Leng Anggia Murni Wijiati Annisa Madyanti Geminastiti Parampasi Anriansyah Renggaman Ardilasunu Wicaksono Arini Resti Fauzi Asah Hilaliah Aulia Andi Mustika Beginer Subhan Chairani Ridha Maghfira Darna Andrian Ramadhan Dea Indriani Astuti Dedi Rahmat Setiadi Deni Noviana Denny Widaya Lukman Denny Widya Hikman Destri Prameswari, Alvira Dinamella Wahjuningrum Diyah Septiriyanti Edi Sukmawinata Edi Sukmawinata Ekowati Handharyani Etih Sudarnika Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Faoziyatunnisa, Nurul Fauzi, Arini Resti Gustilatov, Muhamad Hardianti, Aprilia Hary Krettiawan Islami, Nurfara Julie Ekasari Karen Lee koekoeh santoso Leaw Yi Xin Lydia Pow Kar Men Maria Luisa MNB Klobongona Megan Chan Zhi Ying Merlia Andriyani Mia Setiawati Michella Hoseana Wijaya MUHAMMAD AGIL Muhammad Arif Mulya MUNTI YUHANA Nabila Swarna Puspa Hermana Nauval Firdana, Chorrysa Nor Jannah, Nor Nora Dyah Ayu Purnamaningrum Nugraha, Arifin Budiman Nuri Kamaliah, Syarifah Nursa Rima Putri Rahmat Hidayat Rahmat Hidayat Revita Ismahyuningsih Ridi Arif Risa Tiuria Risqika Akla Velayati Rizal Dwinto Rochman Ronald Tarigan Rotinsulu, Dordia Anindita Safika S, Safika sri murtini . Sri Nuryati Srihadi Agungpriyono Sudrajat, R Herman Suhendi, Adnan Rizal Sukenda, Sukenda, Suleman, Gabriella Augustine Supratikno Supratikno Surya Agus Prihatno Tambun, Andreas Titiek Sunartatie Titiek Sunartatie Trioso Purnawarman Tsani Untsa, Agista Ulandari, Rafika Ulfatin Khoiriyah Herowati Vindriati, Zukhrufa Vista Wicaksono, Baref Agung WIDANARNI WIDANARNI Yohana Tri Astuti Yosua Kristian Adi Yusuf Ridwan