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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Teknologi Industri Pertanian E-jurnal Agro-Industri Indonesia Reaktor Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Agrointek Jurnal Penelitian Pascapanen Pertanian Jurnal Penelitian dan Pengembangan Pertanian Buletin Teknologi Pasca Panen Journal of Engineering and Management in Industrial System Indonesian Journal of Business and Entrepreneurship (IJBE) Journal of Engineering and Technological Sciences agriTECH Jurnal Sumberdaya Hayati Science and Technology Indonesia Jurnal Bioteknologi & Biosains Indonesia (JBBI) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology EDUFORTECH Journal of Tropical Crop Science Menara Perkebunan Journal of Applied Engineering and Technological Science (JAETS) Indonesian Journal of Electrical Engineering and Computer Science Jurnal Penelitian dan Pengembangan Pertanian International Journal of Mechanical Engineering Technologies and Applications (MECHTA) Jurnal Agroekoteknologi Terapan ABDI PUSTAKA: Jurnal Perpustakaan dan Kearsipan Proceedings of International Conference on Multidiciplinary Research Journal of Applied Agricultural Science and Technology Prosiding Seminar Nasional Universitas Serambi Mekkah PERTANIAN TROPIK Pharmaceutical Sciences and Research (PSR) Makara Journal of Science Makara Journal of Technology Jurnal Natural Journal of Sciencce Technology and Visual Culture JURNAL REKAYASA KIMIA & LINGKUNGAN
Titi Candra Sunarti
Departemen Teknologi Industri Pertanian Fakultas Teknologi Pertanian Institut Pertanian Bogor Jl. Lingkar Akademik, Kampus IPB Darmaga, Bogor 16680; Telp. (0251) 8621974
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Journal : Jurnal Penelitian Pascapanen Pertanian

 1 
Nanocellulose synthesis from pineapple fiber and its application as nanofiller in polyvinyl alcohol-based film Iriani, Evi Savitri; Wahyuningsih, Kendri; Sunarti, Titi Candra; Permana, Asep Wawan
Jurnal Pascapanen Pertanian Vol 12, No 1 (2015): Journal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

A variety of attempts have been made to reduce the dependence on petroleum raw materials based plastics which are limited supply and difficult to degrade, so it can cause the environment problems. Polyvinyl Alcohol/PVA, one of the water-soluble polymer, has good compatibility with fillers, i.e., nanocellulose addition, so it can resulted a composite films that are environmentally friendly and the mechanical properties close to conventional plastic. The objectives of research was to know the effect of pineapple nanocellulose fibers addition on the improvement of the mechanical properties of composite films based on polyvinyl alcohol. The method for manufactured of composite films using solution casting. A simple in a one-pot process was carried out by mixing of PVA solution in various concentration of pineapple nanocellulose fibers (10% - 50%) and glycerol with 2 levels (0% and 1%). Films observations were tensile strength, elongation, crystallinity, and morphology. The results shows that the addition of nanocellulose fibers 10-40% were effective in increasing tensile strength and elongation, but the higher addition (of up to 50%) resulted in decreased elongation. The addition of glycerol on the composite film tends to lower the tensile strength and elongation. This is supported by the XRD data showed that the addition of nanocelullose was also effective to improve the crystallinity properties of the films, but the properties of crystallinity decreased after adding glycerol. The best mechanical properties of composite film produced by the treatment on nanocellulose 40% addition and without glycerol.
Bioinsecticides Production by Bacillus thuringiensis subsp. aizawai using Agroindustrial by-Product in Solid Fermentation Kirana Sanggrami Sasmitaloka; Titi Candra Sunarti; Mulyorini Rahayuningsih
Jurnal Penelitian Pascapanen Pertanian Vol 13, No 1 (2016): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v13n1.2016.1-10

Abstract

Bacillus thuringiensis subsp. aizawai is one of well-known bioinsectiside resources, and utilized in organic farming for the replacement of chemical insecticide. The active compounds produced from fermentation process of B. thuringiensis subsp. aizawai are bacterial spores and protein crystal of δ-endotoxins, which are variously toxic to larvae of the Lepidoptera and Coleoptera. The use of bioinsecticides in Indonesia is still rarely because bioinsecticide marketed in Indonesia is still an import product so that price is relatively expensive. This problem can be overcome with producing bioinsecticide contain active B. thuringiensis using agroindustrial by product as a raw materials. This research compared the bioinsecticide production from several agroindustrial by product in solid fermentation of B. thuringiensis subsp. aizawai. This research aimed to find out the ability of B. thuringiensis subsp. aizawai using substrate combination of carbon source (cassava dregs, pulp of coffee, starch fractions of iles-iles, and sago dregs) and nitrogen source (tofu’s waste, peanut meal, palm kernel cake, and corn hominy) in bioinsecticides production using solid media cultivation. The research consisted of three stages, there were substrates characterization, microorganisms characterization, and bioinsecticides production. The main parameters to select the carbon and nitrogen sources are LC50 value and potential bioinsecticides products. The results showed that the best cultivation of B. thuringiensis subsp. aizawai contained in combination cassava dregs and palm kernel cake blends. This cultivation produced total cell counts 11.2 log CFU/g, viable spore counts 8.9 log CFU/g, LC50 0.04 μg/ ml and potential products 20000 IU/mg. Biopesticide produced can be used to kill Crocidolomia binotalis on cabbage. PRODUKSI BIOINSEKTISIDA OLEH Bacillus thuringiensis subs. aizawai PADA KULTIVASI MEDIA PADAT MENGGUNAKAN LIMBAH AGROINDUSTRI Bacillus thuringiensis subsp. aizawai merupakan salah satu bakteri yang digunakan untuk menghasilkan bioinsektisida dan digunakan pada pertanian organik untuk menggantikan pemakaian insektisida kimia. Komponen aktif yang diproduksi dari proses fermentasi B. thuringiensis subsp.aizawai adalah spora dan kristal protein δ-endotoksin, yang bersifat toksin terhadap larva Lepidoptera dan Coleoptera. Penggunaan bioinsektisida di Indonesia masih jarang karena biasanya berupa produk impor yang harganya mahal. Permasalahan ini dapat diselesaikan dengan memproduksi bioinsektisida menggunakan bahan aktif B. thuringiensis dan hasil samping agroindustri sebagai bahan baku. Penelitian ini bertujuan mengkaji kemampuan produksi B. thuringiensis subsp. aizawai pada beberapa jenis substrat sumber C (onggok, kulit kopi, fraksi pati iles-iles, dan ela sagu) dan sumber N (ampas tahu, bungkil kacang tanah, bungkil inti sawit, dan ampok jagung) dalam memproduksi bioinsektisida dari limbah agroindustri. Penelitian ini terdiri dari tiga tahap, yaitu karakterisasi substrat, karakterisasi isolat dan produksi bioinsektisida. Parameter utama untuk menetapkan sumber karbon dan nitrogen yang tepat adalah nilai LC50 dan potensi produk bioinsektisida. Hasil penelitian menunjukkan bahwa produksi bioinsektisida yang terbaik terdapat pada kultivasi B. thuringiensis subsp. aizawai menggunakan kombinasi onggok dan bungkil inti sawit. Pada kultivasi ini dihasilkan jumlah sel hidup 11.2 log CFU/g, jumlah spora 8.9 log CFU/g, nilai LC50 0.04 μg/ ml dan potensi bioinsektisida 20000 IU/mg. Bioinsektisida yang dihasilkan dapat digunakan untuk membasmi larva Croccidolomia binotalis pada tanaman kubis.
PRODUKSI ENZIM SELULASE OLEH AKTINOMISET MENGGUNAKAN FROND SAGU Sumarni Nompo; Anja Meryandini; Titi Candra Sunarti
Jurnal Penelitian Pascapanen Pertanian Vol 16, No 2 (2019): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v16n2.2019.80-89

Abstract

Fround Sagu adalah pucuk batang sagu yang masih dibungkus oleh pelepah dan tidak diamnfaatkan oleh industri pengolahan sagu. Fround sagu memiliki kandungan serat dengan kandungan selulosa yang tinggi serta berpotensi dijadikan bahan baku untuk produksi selulase. Enzim selulase diproduksi melalui kultivasi substrat cair frond sagu oleh Aktinomiset. Subtart berupa tepung sagu dan ampas frond sagu, diinokulasi oleh isolat HJ4 (3b) dan HJ5 (4b). Kedua isolat diremajakan dalam medium ISP-4 selama 5 hari, kemudian diinokulasikan ke dalam media tepung frond sagu dan ampas frond dan diinkubasi dalam shaker pada suhu runag selama 9 hari. Kedua isolat Aktinomiset mampu menghasilkan enzim selulase pada kedua substrat dan metode kultivasi. Isolat HJ4 (3b) dan HJ4 (5b) pada perlakuan kultivasi substrat padat ampas frond sagu menghasilkan aktivitas spesifik yaitu endoglukase (CMCase) tertinggi yaitu 0.314 U mg-1 dan 0.294 U mg-1 dan aktivitas spesifik enzim eksoglukanase (FPase) yaitu 0.269 U mg-1 dan 0.258 U mg-1, sedangkan pada perlakuan kultivasi substat padat menggunakan tepung frond sagu dihasilkan aktivitas spesifik endoglukanase masing-masing sebesar 0.258 U mg-1 dan 0.254 U mg-1 serta aktivitas spesifik eksoglukanase 0.205 U mg-1 dan 0.198 U mg-1. Production of Cellulase Enzyme by Actinomycet Using Sago FrondSago frond is the upper part of sago trunk which is still wrapped by leaflet, and is not used by the sago processing industry. Sago frond contains fiber with high cellulose content that could potentially be used by as raw material for cellulase production. Cellulase enzymes were produced through both solid-state and submerged cultivation of sago frond by Actinomicycetes. Two substrates, sago frond flour and pulp of sago fronds, were inoculated by isolate HJ4 (3b) and HJ4 (5b). Both isolates were rejuvenated in Sp-4 medium for 5 days, then were inoculated into substrate of frond flour and hampas, and were incubated in a shaker at room temperature for 9 days. Both Actinomycetes isolates were able to produce cellulase enzymes by using both substrates and cultivation methods. The isolates of HJ4 (3b) and HJ4 (5b) by using pulp and solid-state cultivation produced the highest endoglucanase (CMCase) specific activity of 0.294 U mg-1 and 0.276 U mg-1 and exoglucanase (FPase) substrate specific activity os 0.252 U mg-1 and 0.241 U mg-1, while in the solid-state cultivation and by using sago fronds flour resulted in specific endoglucanase activities which were 0.242 U mg-1 and 0.238 U mg-1 and exoglucanase specific activities 0.192 U mg-1 and 0.185 U mg-1, respectively.
SINTESIS NANOSELULOSA DARI SERAT NANAS DAN APLIKASINYA SEBAGAINANOFILLERPADA FILM BERBASIS POLIVINIL ALKOHOL Evi Savitri Iriani; Kendri Wahyuningsih; Titi Candra Sunarti; Asep Wawan Permana
Jurnal Penelitian Pascapanen Pertanian Vol 12, No 1 (2015): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v12n1.2015.11-19

Abstract

A variety of attempts have been made to reduce the dependence on petroleum raw materials based plastics which are limited supply the mechanical properties close to conventional plastic. The objectives of research was to know the effect of  pineapple nanocellulose effective in increasing tensile strength and elongation, but the higher addition (of up to 50%) resulted in decreased elongation. The addition and without glycerol
Co-Authors Adisalamun Adisalamun Adriana, Savira Astri Adwiyani, Pustika Agus Setiyono AHMAD JUNAEDI Aisyah, Nadira Akhmad Endang Zainal Hasan Anas Miftah Fauzi Andi Nurul Aulia Sari Andika Susantri Ani Suryani Ani Suryani Ani Suryani Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anja Meryandini Anuraga Jayanegara Aris Purwanto Asep Wawan Permana Asep Wawan Permana, Asep Wawan Asrianti Basri Asrianti Basri Ayu Rahayu SARASWATI Bambang Prasetya Basri, Asrianti Basri, Asrianti Besty Maranatha Birahy, Deford Cristy Bruce A. Welt, Bruce A. Cahyana, Purwa Tri Christina Winarti Christina Winarti Dede Heri Yuli Yanto Dedi Fardiaz Devi Ambarwaty Oktavia Dewi Diniaty Djumali Mangunwidjaja Djumali Mangunwidjaja Djumali Mangunwidjaja Djumali Mangunwidjaja Djumali Mangunwidjaja Dwi Setyaningsih Eka Putri EKA RURIANI EKA RURIANI Eka Ruriani Elisa Julianti Elly Rosyidah Endang Warsiki Endang Warsiki Erliza Noor Euis Hermiati Evi Savitri Iriani Evi Savitri Iriani, Evi Savitri Faqih Udin FARAH FAHMA Fauzan, RM Muhammad Nur Februadi Bastian Feri Kusnandar Ferry Mutia Fiora Helmi Frangky J. Paat Glisina Dwinoor Rembulan Hadi Karia Purwadaria, Hadi Karia Hari Eko Irianto Hartami Dewi Hartrisari Hardjomidjojo Hasrul Satria Hasrul Satria, Hasrul Ifah Munifah Ika Amalia Kartika Ilham Marvie Indah Yuliasih IRA ERDIANDINI, IRA Irmayanti Irmayanti Irmayanti Irmayanti, Irmayanti Irvan Anwar Iskandar Lubis Kendri Wahyuningsih Kendri Wahyuningsih, Kendri Khaswar Syamsu Kirana Sanggrami Sasmitaloka Kurniawan Yuniarto Kurniawan, Dede Yudo Laily, Noer Lala Nurmala Machfud Machfud Mala Nurilmala Manalu, Lamhot Maranatha, Besty Marimin , Matasik, Delfania Maya Melati Mohamad Syamsul Ma’arif Mulyorini Rahayuningsih MUTIA, FERY NAOMI, APRILIA Nastiti Siswi Indrasti Nida El Husna Niken Financia Gusmawati NISA RACHMANIA MUBARIK Nisfatin Shofiana Nur Richana Nuri Andarwulan Odi Andanu Ono Suparno Paramuji, Muji Pasaribu, Fatimah J Petrus Adam Pramana, Yanuar Sigit Purwanto, Yanuar Jarwadi Purwoko Purwoko RAMADHAN, ZULFA AULIA Ratna Ekawati Ratna Ekawati Ridwansyah Ridwansyah Rijanti Rahaju Maulani Rijanti Rahaju Maulani Rijanti Rahaju Maulani Rini Purnawati Rismawati . Roh santoso Ronny Soputan Rosyidah, Elly Ruka Yulia SAFITRI NURLAELA Salfauqi Nurman, Salfauqi Sanbein, Primus Sapta Raharja Setyo Purwanto Singgih Wibowo Sitti Rahbiah Akram SOFIYANTO, M. EDY Stanislaus Aditya Agung Sugiyanta Sukard Sumarni Nompo Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suprihatin Suryanegara, Lisman Suyono, Meisy Nawang Taufik Hidayat Tjahja Muhandri Tryanisa Ridla Amalia Utomo, Prasetyo Hadi Vestika Iskawati Wahidul Hijah Wahyu Widosari Wawan Hermawan Widiatmaka Widiatmaka Widosari, Wahyu WULAN, RAHAYU Yandra Arkeman Yaya Rukayadi Yuana Susmiati Yulia, Ruka YULIANA, META YULIN LESTARI Yulistika, Efri Yunus Triyonggo, Yunus Yusmiati Yusmiati Zahrani, Siti Mutia