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All Journal e-Journal Pustaka Kesehatan Current Biochemistry Program Kreativitas Mahasiswa - Penelitian Biology, Medicine, & Natural Product Chemistry Jurnal Perlindungan Tanaman Indonesia Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) BERITA BIOLOGI Biodidaktika : Jurnal Biologi dan Pembelajarannya Jurnal Pengabdian Pada Masyarakat Jurnal Biologi Tropis Gorontalo Agriculture Technology Journal Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Ilmiah Farmasi Farmasyifa Jurnal Lingkungan dan Sumberdaya Alam (JURNALIS) International Journal Mathla'ul Anwar of Halal Issues Biological Environment and Pollution Jurnal Pengabdian Pada Masyarakat Jurnal Ilmiah Kesehatan Delima Jurnal Ilmu Pertanian dan Perkebunan Jurnal Kesehatan Tujuh Belas (Jurkes TB)
Hadi Susilo
Universitas Mathla'ul Anwar
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Journal : BERITA BIOLOGI

 1 
ISOLASI GEN SITRAT SINTASE BAKTERI Pseudomonas aerugenosa PS2 DARI RIZOSFER POHON KRUING (Dipterocarpus sp.) UNTUK MODEL KONSTRUKSI METABOLISME SEL MIKROALGA BERKARBOHIDRAT RENDAH Susilaningsih, Dwi; Umoro, Asahedi; Ochieng, Fredrick Onyango; Widyaningrum, Dian Noverita; Susanti, Hani; Susilo, Hadi; Swastika, I Nengah; Widyastuti, Utut
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4410.754 KB) | DOI: 10.14203/beritabiologi.v18i2.2967

Abstract

Pseudomonas has the potential ability for production of citrate synthase synthesis. Pseudomonas aeruginosa could synthesize the enzyme of citrate synthase which is most likely compatible with microalgae cell. Pseudomonas aerugenosa can be found in the rhizosphere of Kruing (Dipterocarpus sp., Dipterocarpaceae). This bacteria is commonly used in agriculture purposes because it is able to synthesize organic acid such as citric acid. These organic acids are synthesized from a reaction between oxaloacetate and acetyl CoA, catalyzed by citrate synthase (CS) in the tricarboxylic acid cycle (TCA). Rhizosphere as microbial sources was obtained from Kruing (Dipterocarpus sp.), which was collected from ?Carita? Research Forest, Pandeglang, Banten, West Java. Citrate synthase gene-specific primers were designed based on citrate synthase gene sequences as depicted in Genbank. The isolation and amplification showed that citrate synthase can be detected and purified from Pseudomonas aeruginosa target and it consists of 1600 bp and encodes 509 amino acids. Based on BLAST (Basic Local Alignment Search Tool) analysis, CS genes that were successfully isolated had 92 % similarity with Pseudomonas aeruginosa type II citrate synthase. This CS gene is expected to be expressed in microalgae metabolism to divert the metabolism of carbohydrate formation into fatty acids. 
ISOLASI GEN SITRAT SINTASE BAKTERI Pseudomonas aerugenosa PS2 DARI RIZOSFER POHON KRUING (Dipterocarpus sp.) UNTUK MODEL KONSTRUKSI METABOLISME SEL MIKROALGA BERKARBOHIDRAT RENDAH Dwi Susilaningsih; Asahedi Umoro; Fredrick Onyango Ochieng; Dian Noverita Widyaningrum; Hani Susanti; Hadi Susilo; I Nengah Swastika; Utut Widyastuti
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.2967

Abstract

Pseudomonas has the potential ability for production of citrate synthase synthesis. Pseudomonas aeruginosa could synthesize the enzyme of citrate synthase which is most likely compatible with microalgae cell. Pseudomonas aerugenosa can be found in the rhizosphere of Kruing (Dipterocarpus sp., Dipterocarpaceae). This bacteria is commonly used in agriculture purposes because it is able to synthesize organic acid such as citric acid. These organic acids are synthesized from a reaction between oxaloacetate and acetyl CoA, catalyzed by citrate synthase (CS) in the tricarboxylic acid cycle (TCA). Rhizosphere as microbial sources was obtained from Kruing (Dipterocarpus sp.), which was collected from ‘Carita’ Research Forest, Pandeglang, Banten, West Java. Citrate synthase gene-specific primers were designed based on citrate synthase gene sequences as depicted in Genbank. The isolation and amplification showed that citrate synthase can be detected and purified from Pseudomonas aeruginosa target and it consists of 1600 bp and encodes 509 amino acids. Based on BLAST (Basic Local Alignment Search Tool) analysis, CS genes that were successfully isolated had 92 % similarity with Pseudomonas aeruginosa type II citrate synthase. This CS gene is expected to be expressed in microalgae metabolism to divert the metabolism of carbohydrate formation into fatty acids. 
Co-Authors , Triadiati A Suhaemi AA Sudharmawan, AA Abdilah, Nurullah Asep Abdul Hasyim Sodiq, Abdul Hasyim Agung Sugiarto Agus Setiawan Ai Widiyawati Aji Sukoco Ali Farkhan Tsani Andrianto Heri Wibowo Arif, Hamid Arini Khaerunnisa Asahedi Umoro Ayu Mira Yunita Candra Junaedi Cory Novi Dedeh Jubaedah Dhyneu Dwi Jayantie Dian Noverita Widyaningrum Dimas Danang Indriatmoko Dwi Marwati Juli Siswanti DWI SUSILANINGSIH Dwi Susilaningsih Eka Yulli Kartika Eneng Elda Ernawati Faizal Andri Fredrick Onyango Ochieng Gina Siti Nurhayati Hani Susanti Hani Susanti, Hani Herlina Siti Nurantika Hidayat, Deden Hudaya, Dadan Ahmad Hujaji, M. I Nengah Swastika Imat Maftuhah Indriati, Marlinda irdaus, Ahmad F irfan mujahidin Kartina Kartina Kiki Rizki Amelia Kurnia, Nia Marlina Lia Amelia Pertiwi M Nasrun Hakim M. Muslim Maritha, Vevi Marlinda Indriati Merdiana Indah Saputri Mu'jijah Mu'jijah Mu'jijah, Mu'jijah Mu’jijah, Mu’jijah Neli Nailul Wardah NISA RACHMANIA MUBARIK Nurafliyan Susanti, Ervi Nurmayulis Nuryati Nuryati Ochieng, Fredrick Onyango Rahmadanti, Aulia Rahmawati, Dania Ratna Fitry Yenny Renni Dwi Saputri Riki Rikardo Riki Rikardo Rizky, Robby Roihwan Roihwan Rosmawaty Peranginangin Roudotussaadah Roudotussaadah Rt Inu Rahayu S, Dwi Marwati Juli Sari, Ruly Nadian Sibawi Sibawi, Sibawi Siti Yumsinah Sri Setiyowati sudanto sudanto Suryandani, Hendy Suryanti Suryanti Susilawati, Susilawati Susiyanti, Susiyanti Suyamto Suyamto Suyamto Swastika Oktavia Swastika, I Nengah Syahbana, Mohammad Ana Tarso Rudiana, Tarso Taufich Hilman, Taufich Trisnawati, Desi Tuti Rostianti Maulani Umoro, Asahedi Umurohmi, Ulfah Usman Setiawan UTUT WIDYASTUTI UTUT WIDYASTUTI Widyaningrum, Dian Noverita Yelfi Yelfi Yuniarsih, Eko Yuningsih Yuningsih Yusransyah, Yusransyah