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All Journal HAYATI Journal of Biosciences Buletin Agrohorti Jurnal Biofisika Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Teknologi Industri Pertanian ACTA VETERINARIA INDONESIANA Jurnal Ilmu dan Teknologi Hasil Hutan Jurnal Pengolahan Hasil Perikanan Indonesia VALENSI Jurnal Veteriner International Journal of Renewable Energy Development JURNAL KIMIA SAINS DAN APLIKASI Jurnal Ilmu Dasar POSITRON Jurnal Penelitian Fisika dan Aplikasinya (JPFA) Jurnal Sains Materi Indonesia Jurnal Keteknikan Pertanian Jurnal Penelitian Hasil Hutan Buletin Penelitian Tanaman Rempah dan Obat Teknik dan Ilmu Komputer Jurnal Matematika & Sains Rekayasa Mesin JFA (Jurnal Fisika dan Aplikasinya) Jurnal Spektra Jurnal Agro JOURNAL ONLINE OF PHYSICS Omega: Jurnal Fisika dan Pendidikan Fisika Jurnal Fisika FLUX JMPM (Jurnal Material dan Proses Manufaktur) Science and Technology Indonesia Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Prosiding Seminar Nasional Teknoka Indonesian Journal of Chemistry Jurnal Purifikasi Jurnal Ilmu Fisika Jambura Physics Journal Jurnal Ilmu dan Teknologi Kayu Tropis JCPS (Journal of Current Pharmaceutical Sciences) JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Jurnal Fisika dan Terapannya Jurnal Penelitian Hasil Hutan Buletin Penelitian Tanaman Rempah dan Obat Wood Research Journal : Journal of Indonesian Wood Research Society Wood Research Journal : Journal of Indonesian Wood Research Society Makara Journal of Science Makara Journal of Technology Jurnal Ilmiah Sains Time in Physics: Journal for Theoretical, Instrumentation, Material-Molecular, and Education Physics Radial : Jurnal Ilmiah Sains Dan Rekayasa International Journal of Renewable Energy Development Wahana Fisika : Jurnal Fisika dan Terapannya Jurnal Sains Materi Indonesia Jurnal Penelitian Hasil Hutan
Akhiruddin Maddu
Departemen Fisika, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Dramaga, Jalan Agatis, Bogor 16680 Jawa Barat
Agriculture, Biological Sciences & Forestry Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Veterinary

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Journal : HAYATI Journal of Biosciences

 1 
Activity and Stability of the Alcohol Biosensor Using Acetobacter aceti Biofilm on Screen-Printed Carbon Electrode Dyah Iswantini; Fitriani Indahsari; Akhiruddin Maddu; Novik Nurhidayat; Henny Purwaningsih; Sri Sugiarti
HAYATI Journal of Biosciences Vol. 27 No. 1 (2020): January 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (865.056 KB) | DOI: 10.4308/hjb.27.1.24

Abstract

Most of the alcohol analytical methods are robust and instrumentally expensive. An alternative of ethanol biosensor based on selected biofilm forming Acetobacter aceti bacteria producing alcohol oxidase was constructed on a screen-printed carbon electrode. The enzyme specifically oxidizes the ethanol and generate electrical current that then electrochemically detected and measured by cyclic voltammetry method. A scanning electron microscopic analysis indicated that the biofilm was formed firmly in the electrode. This constructed biosensor reached its optimum at biofilm formed by bacteria of 1.33 × 1010 cells/ml, temperature of 27°C, and pH 7. The enzyme kinetic had KM and Vmax AOX values of 3.5 mm and 125 μA respectively. The biosensor had detection and quantization limit of 0.003 and 0.009%, respectively, and a sensitivity of 57.29 μA (%)-1. A linearity and relative deviation value were revealed at 0.993 and 1.95% respectively. The biosensor was relatively specific and had no interferences with methanol, sodium chloride and citric acid as the common interferences of ethanol compounds. Furthermore, the biosensor had been stably for at least 55 days. Therefore, this constructed biosensor should be developed into a prototype for a practical effective analysis.
Co-Authors - - Nurrahman . Sugianto Abdulloh Rifai Agus Ghautsun Niam Agus Sukarto Wismogroho Agus Sukarto Wismogroho, Agus Sukarto Ahmad Aminuddin Ahmad Sulaeman Ahmad, Faozan Almiahsari, Ashri Aminuddin Aminuddin Andhika Prima Prasetyo Anita Firmanti Aribah, Putri Auliya Ilmiawati Bagus Irawan Bagus MARTIANTO Baihaqi, Hans Bambang - Riyanto Bambang Herawan Bambang Herawan, Bambang Bambang Riyanto Bambang Sugeng Bonny Poernomo Wahyu Soekarno Budi Arifin Candra A. Basuki Charlena Cherly Firdarini Cherly Firdharini Dede Hermawan Deded Sarip Nawawi Deded Sarip Nawawi Deni Shidqi Khaerudini Dimas Ramadhan Putra Dimas Ramadhan Putra Dimyati , Arbi Djoko Pujiarto Dodyk Pranowo Dyah Iswantini Elbersen, Wolter Erliza Noor Esa Ghanim Fadhallah Esa Ghanim Fadhallah Farly Reynol Tumimomor Fayakun, Kun Firdarini, Cherly Firdharini, Cherly Fitri Viva Yuningsih, Aida Fitriani Indahsari Gareso, Paulus P. Gareso, Paulus P. Gustan Pari Gustan Pari Gustan Pari Gustan Pari Gustan Pari Gustan Pari Gustini Syahbirin Hamdani Zain Hamdani Zain Hamdani Zain Hamdani Zain Hans Baihaqi Hardhienata, Hendradi Harry Ramza Harry Ramza Henny Purwaningsih HERA MAHESHWARI Hera Maheswari, Hera Herry Suhardiyanto Horasdia Saragih Huda Shalahudin Darusman Husin Alatas Ida Ayu Putu Sri Widnyani ikhwan wirahadikesuma Ikhwan Wirahadikesuma Imelia Dewi Inda Setyawati, Inda Indah Puspita, Indah Ira Ukhtiningsih Ira Ukhtiningsih, Ira Irmansyah . Irmansyah Irmansyah Irmansyah Irmansyah Irmansyah Irmansyah Irzaman, Irzaman Ismail Budiman Ismail Budiman Isnaeni Isnaeni Isnaeni Isnaeni Isnaeni Isnaeni Isnaeni Jayadi Jayadi Jayadi Jayadi Jayadi Jayadi Jhav Sund Oktoricoento Johan D. Sirait Jufra Jufra Jumardin jumardin jumardin Kadapi, Muhamar Kiagus Dahlan koekoeh santoso Kokoeh Santoso Kudang Boro Seminar Kun Fayakun Kun Modjahidin Kun Modjahidin, Kun La Ode Muliadi LAKSMI AMBARSARI Liesbetini Haditjaroko Lina Karlinasari Luthfan Irfana M. Farouq Iksan Mahfuddin Zuhri Marga Asta Jaya Mulya Melinia Falestin Mersi Kurniati Mia Fitriana, And Moehamad Barmawi Mohamad Rahmad Suhartanto Mohammad Mujirudin Muhammad F Ikhsan Muhammad Ramdani Muhammad Rizki Mujirudin, Mohammad Mulya, Marga Asta Jaya Nafisah Nafisah Novik Nurhidayat Nur Adi Saputra Nur Adi Saputra Nurfina Yudasari Nurul Rostami Dewi Nurul Rostami Dewi Oktarina Heriyani Oktoricoento, Jhav Sund Paulus P Gareso, Paulus P Pepen Arifin Permono Adi Putro Pramudito, Sidikrubadi Purawiardi , Ibrahim Purwantiningsih Sugita Purwantiningsih Sugita Rahman, Mohammad Syuhaimi Ab Rahmani, Nabiila Rahmaniah Nalwi Rahmaniah Nalwi Ramdani, Muhammad Ramza, Harry Rasyida Ulfa Ratna Sari Dewi Riano, Riza Rini Kurniasih, Rini Rinto Paputungan Rosniyati Suwarda Rustami, Erus Safaruddin A Prasad Sahrudin Sahrudin Sahrudin, Sahrudin Sar Sardy Sar Sardy Sar Sardy Sari, Yessie Setyanto Tri Wahyudi Siti Nikmatin Sofian, Irmansyah Subyakto Subyakto SUBYAKTO SUBYAKTO Sugianto Arjo Sugianto, . Sugianto, . Sugianto, Sugianto Suhartanto, Muhammad Rahmad Sulaeman, Ahmad Sofyan Sulistioso Giat Sukaryo, Sulistioso Giat Sultan, Syamsu Supian, Latifah Sarah Supriyanto . Taqiyyah, Izzatut Tarcisius Rio Mardikanto Tarigan, Putri Andayati Br Tatang Hidayat Wahyu Bambang Widayatno Wahyu Bambang Widayatno, Wahyu Bambang Wasmen Manalu Wasrin Syafii Widaningrum Winarti , Christina wirahadikesuma, ikhwan Wirahadikesuma, Ikhwan Wirahadikesuma Yayan Firmansyah Yessie Sari Yessie Widya Sari Yogi Waldingga Hasnedi Yulia Irdawati Yulia Sani Yulia Sani, Yulia Zetria Zikri