Anja Meryandini
Department Of Biology, Faculty Of Mathematics And Natural Sciences, IPB University, IPB Darmaga Campus, Bogor 16680; Center For Biotechnology, International Research Institute, IPB University, IPB Darmaga Campus, Bogor 16680

Published : 119 Documents Claim Missing Document
Claim Missing Document
Check
Articles

PENINGKATAN KUALITAS BIJI KAKAO (Theobroma cacao L) MELALUI FERMENTASI MENGGUNAKAN Lactobacillus sp. dan Pichia kudriavzevii Meryandini, Anja; Basri, Asrianti; Sunarti, Titi Candra
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (848.014 KB) | DOI: 10.29122/jbbi.v6i1.3048

Abstract

The Improvement of Cacao Beans Quality through Fermentation by Using Lactobacillus sp. and Pichia kudriavzeviiABSTRACTIndonesia is one of the main cacao producers in the world. Indonesian cacao product is, however, relatively of low quality. Quality improvement of cacao beans is thus needed to increase added value of the product through such method as fermentation using bacteria and yeast. This study was conducted using four fermentation treatments, namely F1 (spontaneous fermentation without the addition of inoculum), F2 (addition of lactic acid bacteria inoculum), F3 (addition of yeast inoculum), F4 (addition of mixed lactic acid bacteria and yeast inoculum). The fermentation was carried out for 5 days. The parameters measured were the microbial cell number, pH, ethanol, total reducing sugar, and total acid concentration, as well as cacao seed quality. Results showed that, compared to the other treatments, the F4 treatment gave the best result, namely 83% of the cacao seeds being fermented, 2% non-fermented, 14% unfermented, 1% moldy, and 2% germinated. The liquid produced during the fermentation contained the highest reducing sugar of 123.38 mg·mL-1, the highest total acid of 24.42 mg·mL-1, and 3.57% ethanol.Keywords: cacao beans, fermentation, lactic acid bacteria, starter, yeast ABSTRAKIndonesia adalah salah satu penghasil kakao utama di dunia. Namun berdasarkan mutu, produk kakao Indonesia masih relatif tergolong rendah. Peningkatan kualitas biji kakao diperlukan untuk memberikan nilai tambah pada produk melalui metode seperti fermentasi menggunakan bakteri dan khamir. Penelitian ini dilakukan dengan empat perlakuan fermentasi yaitu F1 (fermentasi secara spontan tanpa penambahan inokulum), F2 (dengan penambahan inokulum bakteri asam laktat (BAL)), F3 (dengan penambahan inokulum khamir), F4 (dengan penambahan inokulum campuran bakteri asam laktat dan khamir). Fermentasi dilakukan selama 5 hari, dan parameter yang diukur selama fermentasi adalah jumlah mikroba, pH, kadar etanol, gula pereduksi, total asam serta kualitas biji. Hasil menunjukkan bahwa, dibandingkan perlakuan lainnya, perlakuan F4 memberikan hasil terbaik yaitu 83% biji terfermentasi, 2% tidak terfermentasi, 14% terfermentasi sebagian, 1% berjamur, dan 2% berkecambah. Cairan fermentasi tersebut mengandung gula reduksi yang paling tinggi 123,38 mg·mL-1, total asam tertinggi 24,42 mg·mL-1, dan kadar etanol mencapai 3,57%.Kata Kunci: bakteri asam laktat (BAL), biji kakao, fermentasi, khamir, starter
Characterization of Xylanase Streptomyces spp. SKK1-8 ANJA MERYANDINI; TRIO HENDARWIN; DEDEN SAPRUDIN; YULIN LESTARI
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.007 KB) | DOI: 10.4308/hjb.13.4.151

Abstract

Streptomyces spp. SKK1-8 producing xylanase was isolated from soil sample from Sukabumi West Java. The xylanase have an optimum condition at pH 6 and 50 0C. Addition of 5 mM Cu2+ decreased the xylanase activity up to about 77%, whereas not by other cations. The xylanase was stable at 3 0C for 48 hours, and the enzyme half lifetime was 1 hour 45 minute at 50 0C. This xylanase showed the highest activity on oatspelt xylan, and their molecular masses were estimated approximately 16.80, 15.21, and 13.86 kDa. HPLC analysis showed that xylosa and arabinosa were the main hydrolytic product of birchwood xylan. Key words: xilanase, Streptomyces spp., characterization, zymogram and SDS-PAGE, stability
Characterization of Xylanase from Streptomyces spp. Strain C1-3 ANJA MERYANDINI
HAYATI Journal of Biosciences Vol. 14 No. 3 (2007): September 2007
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (52.565 KB) | DOI: 10.4308/hjb.14.3.115

Abstract

Xylan is the major constituent of hemi cellulose. Several enzymes are needed to hydrolyse xylan completely, including xylanase. Currently, there is an increasing use of this enzyme. This study was carried out to characterize the xylanase from Streptomyces spp. strain C1-3. Results showed that the xylanase displayed its highest activity at pH 3 and 90 0C and was stable up to 10 hours at this conditions. Its activity increased after the addition of Cu2+, Fe2+, and Co2+ under concentration of 1 and 5 mM, respectively. The activity however, decreased after the addition of Mg2+, Ca2+ at 1 mM and Zn2+ at 5 mM. After a test with five kinds of xylan (i.e. from Birchwood, Beechwood, Arabinoxylan, Oat spelt and CMC), the xylanase of Streptomyces spp. C1-3 showed its preferences to Birchwood- and Arabino-xylan. The results showed that the xylanase of Streptomyces spp. C1-3 was characterized as a thermostable acid xylanase. Key words: xylanase, Streptomyces, stability, CMCase
Yeast Isolation for Bioethanol Production EKA RURIANI; TITI CANDRA SUNARTI; ANJA MERYANDINI
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (53.245 KB) | DOI: 10.4308/hjb.19.3.145

Abstract

We have isolated 12 yeast isolates from five different rotten fruits by using a yeast glucose chloramphenicol agar (YGCA) medium supplemented with tetracycline. From pre-screening assay, four isolates exhibited higher substrate (glucose-xylose) consumption efficiency in the reaction tube fermentation compared to Saccharomyces cerevisiae dan Saccharomyces ellipsoids as the reference strains. Based on the fermentation process in gooseneck flasks, we observed that two isolates (K and SB) showed high fermentation efficiency both in sole glucose and mixed glucose-xylose substrate. Moreover, isolates K and SB produced relatively identical level of ethanol concentration compared to the reference strains. Isolates H and MP could only produce high levels of ethanol in glucose fermentation, while only half of that amount of ethanol was detected in glucose-xylose fermentation. Isolate K and SB were identified as Pichia kudriavzeevii (100%) based on large sub unit (LSU) ribosomal DNA D1/D2 region.
Characterization of Xylanase activity produced by Paenibacillus sp. XJ18 from TNBD Jambi, Indonesia . KURRATAA’YUN; . YOPI; ANJA MERYANDINI
HAYATI Journal of Biosciences Vol. 22 No. 1 (2015): January 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1681.551 KB) | DOI: 10.4308/hjb.22.1.20

Abstract

Lignocellulose waste in nature is increasing due to the increasing activity of agroforestry. Up to 40% of lignocellulose biomass are consisted of xylan. Xylan complete breakdown requires the action of xylanase. Xylanase has been used to breakdown xylan into commercial product such as low calories sugar, prebiotic, and biofuel. Due to its wide application, several variation of xylanase characterization are needed. Our previous studies have collected Paenibacillus sp. XJ18 from TNBD forest, Jambi, Indonesia, to gain a unique enzyme characteristic. In this study the characteristic of crude xylanase from Paenibacillus sp. XJ18 was investigated. The highest activity of xylanase production was at 36 h. The xylanase showed activity in a broad range of pH (4.5-9.0). The highest activity showed at pH 5.0, 90 oC. Crude enzyme extract was unstable and had halftime at its pH and optimum temperature about 67 min. The xylanase activity was increased about 4.59 times after being concentrated by 70% acetone  (2.4578 U/mL). Based on TLC result, xylanase from Paenibacillus sp. XJ18 was predicted to produce xylobiose exclusively from extracted corncob xylan.
Enzymatic Hydrolysis of Copra Meal by Mannanase from Streptomyces sp. BF3.1 for The Production of Mannooligosaccharides . ARIANDI; . YOPI; ANJA MERYANDINI
HAYATI Journal of Biosciences Vol. 22 No. 2 (2015): April 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1826.954 KB) | DOI: 10.4308/hjb.22.2.79

Abstract

Copra meal contained high polysaccharide mannan. Mannanase Streptomyces sp. BF3.1 efficiently hydrolyzed copra meal to mannooligosaccharides. This research determined the optimum conditions of enzyme mannanase Streptomyces sp. BF3.1 to hydrolyze copra meal. The results of the hydrolysis products were analyzed concentrations of reducing sugars, total sugars and the degree of polymerization. In order to determine the type of product, mannooligosaccharides  were analyzed by thin layer chromatography and high performance liquid chromatography. The mannanase had an optimum condition at 70 °C and pH 6. Optimum conditions of hydrolysis was 10% copra meal concentration with incubation time of 5 h at 30 °C which able to produce a variety of mannooligosaccharides products. Under such conditions, the yield of reducing sugar was 3.83 mg/mL with polymerization degree of 4. Analysis of mannooligosaccharides by thin layer chromatography and high performance liquid chromatography revealed mannobiose, mannotriose, mannotetrose, mannopentose, and mannoheksose.
Effect of Probiotic Bacillus megaterium PTB 1.4 on the Population of Intestinal Microflora, Digestive Enzyme Activity and the Growth of Catfish (Clarias sp.) Wahyu Afrilasari; . Widanarni; Anja Meryandini
HAYATI Journal of Biosciences Vol. 23 No. 4 (2016): October 2016
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (544.994 KB) | DOI: 10.4308/hjb.23.4.168

Abstract

This study aimed to analyze the effect of Bacillus megaterium PTB 1.4 on the population of intestinal microflora, digestive enzyme activity, and the growth of catfish. Gnotobiotic and normal fish were used. Treatment using gnotobiotic was divided into gnoto (with feed and 100 μg/mL rifampicin) and gnotoplus (with feed, 100 μg/mL rifampicin, and 1% probiotic); whereas treatment using normal fish was divided into normalplus (with feed and 1% probiotic) and normal (only feed). The amount of bacteria on gastrointestinal tract was measured 30 days after treatments using the total plate count method. The results indicated no significant difference in bacterial growth between gnotobiotic and normal fish. The total amount of probiotic bacteria with normalplus treatment was significantly different with gnotoplus. The activity of protease and amylase enzymes, and specific growth rate in normalplus treatment were significantly higher (p < 0.05) than other treatments. Bacillus megaterium PTB 1.4 increased the activity of digestive enzymes and the growth of catfish.
Biofungicide Producing Bacteria: an In Vitro Inhibitor of Ganoderma boninense Ade Irma; Anja Meryandini; Bedah Rupaedah
HAYATI Journal of Biosciences Vol. 25 No. 4 (2018): October 2018
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (499.654 KB) | DOI: 10.4308/hjb.25.4.151

Abstract

Oil palm is widely known as one of vegetable oil sources and the main comodity in Indonesian agriculture because of the benefits in non-food and food industries. Ganoderma boninense attack results in considerable losses to agriculture. Chemical control creates a harmful effect on health and the environment. Biocontrol is required to take over the function of chemical control. This study aimed to select bacteria that produce bioactive compounds as biofungicide against G. boninense pathogenic fungi and identify bacteria producing biofungicide using molecular method. The stages of bacterial isolate selection were performed through the selected hemolysis and isolate tests in the antagonistic test. Bacteria were extracted using ethyl acetate and their extract activity were tested. Analysis of bioactive compounds was conducted using thin layer chromatography (TLC) and the identification was based on 16S rRNA gene. The result of bacterial pathogenic test was obtained from two selected bacterial isolates namely 11B LB and 11B MD. Both bacterial isolates showed antagonistic effects by forming an inhibitory zone against G. boninense growth with percentage of inhibitor of 81 and 75%. Activity test of bacterial extract showed that crude extract of bacterial isolate 11B MD had the highest inhibitor activity that is 88.34%. TLC analysis proved that the active extract of bacteria containing metabolite compounds had Rf value of 0.1, 0.28, and 0.38. Isolate bacteria 11B MD was identified as Pseudomonas aeruginosa.
Production and Characteristics of Yeast Dextranase from Soil Vestika Iskawati Wahidul Hijah; Titi Candra Sunarti; Anja Meryandini
HAYATI Journal of Biosciences Vol. 26 No. 1 (2019): January 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (543.647 KB) | DOI: 10.4308/hjb.26.1.26

Abstract

  The existence of dextran in sugar cane juice is a major problem in the sugar industry, causing substantial losses. Treatment of dextran through enzymatic hydrolysis using dextranase is highly recommended as the most suitable method at this time because this is more effective and more economical. This study investigated the production and characterization of dextranase from local isolate yeast to degrade dextran on sugar cane juice. The selected yeast was identified on the basis of molecular identification. Dextranase was produced from the culture with the best carbon and nitrogen sources then was characterized. Application of enzyme was also evaluated. As a selected isolate, F4 had the closest relationship with Pichia kudriavzevii. The highest production of dextranase was induced by the supplementation of glucose and combination of yeast extract and peptone. The enzyme had optimum working condition at pH 7, temperature at 30°C and it is more stable at 4°C of storage temperature. The cation Na+ played key role as co-factor while K+ and Ca2+ were detected as inhibitor of the enzyme. Dextranase from F4 isolate can hydrolyze dextran both in pure and in mixed dextran substrate, but with a lower hydrolysis rate.
Community of Soil Actinobacteria in PTPN VI Oil Palm Plantation Jambi (Sumatra, Indonesia) Based on Amplicon Sequencing of 16S rRNA Gene Mazidah Noer Inayah; Yulin Lestari; Anja Meryandini
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.389-398

Abstract

In Sumatra, Indonesia, increased oil palm production encourages land expansion for oil palm plantations. And soil Actinobacteria have a potential role in agriculture and plantations ecosystems. The use of fertilizer and herbicide affects soil microbial diversity, including Actinobacteria. This research analyzed and investigated the community composition and diversity of Actinobacteria in soils of oil palm plantations in Jambi Sumatra. Amplicon-based analysis of the 16S rRNA gene (V3-V4 hypervariable region) was used to amplify actinobacterial full-length 16S sequences. The V3-V4 actinobacterial specific 16S rRNA gene sequencing was done using Next-Generation Sequencing. This study confirmed that actinobacterial specific 16S rRNA gene primer could amplify the actinobacterial 16S rRNA gene. Frankiales dominated the community composition of soilborne Actinobacteria. The diversity and community composition of soilborne Actinobacteria were not significantly affected by the interaction between fertilization and weed treatments. Furthermore, the use of NPK fertilizer significantly affected the abundance of Kineosporiales, whose abundance increased with the increasing concentration of NPK fertilizer. The interaction between fertilization and weeding treatments in the oil palm plantations has no impact on soil Actinobacteria's community composition and diversity.
Co-Authors . Hamim . KURRATAA’YUN . YOPI . YOPI ., Yopi ., Yopi A, Gading Wilda A, Gading Wilda Abd. Rasyid Syamsuri Abdjad Asih Nawangsih Adawiah, Adilah Ade Andriani ADE ANDRIANI Ade Irma Ahmad Thontowi Aisyah, Nadira Alfred Michael ALINA AKHDIYA Alina Akhdiya AMOR TRESNA KARYAWATI Amor Tresna Karyawati, Amor Tresna Andika Susantri ANGGRAINI PUTRI UTAMI Anggreandari, Rizky Antonius Suwanto Anwar, Irvan Apon Zaenal Mustopa APRILIA NAOMI Ardana Kurniaji Ariandi Arina Amalia Putri Aris Tri Wahyudi Ariyanto, Yogy Satria Armita, Dea Asrianti Basri Atit Kanti Azizah Hikma Safitri Bambang Prasetya Bambang Prasetya Basri, Asrianti Basri, Asrianti Bedah Rupaedah Besty Maranatha CAHYA PRIHATNA Candra Sunarti , Titi Cocok Ana Maryani Berutu Cynthia Rizka Riani DAROJATUL ULYA Dede Heri Yuli Yanto Deden Saprudin Dedy Duryadi Solihin DEDY DURYADI SOLIHIN Deford Cristy Birahy DERI YURATMOKO DEWI APRI ASTUTI Dewi Asnita Dewi, Fitria Diana, Milsa Solva DIMAS ANDRIANTO DINI NURDIANI Dwi Ambarawati Dwi Andreas Santosa DWI SURYANTO DWI SURYANTO Dyah Iswantini Eka Putri EKA RURIANI Elly Rosyidah Engelhaupt, Martin Esti Utarti FAHRRUROZI FAHRRUROZI FAHRURROZI FAHRURROZI Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Fathiah, Muhammad Fadhil Fathin Hamida FATURRAHMAN FATURRAHMAN Faturrahman Faturrahman Ferry Mutia FERY MUTIA Fitri, Ainissya Fitria Dewi Glisina Dwinoor Rembulan GRISELDA HERMAN NATADIPUTRI Hairani, Atikah Hamim Hamim Hamtini - Hamtini Hanni Tsaaqifah Hari Eko Irianto Hartono, Faisal Diniamal Hasrul Satria Hasrul Satria Nur Hasrul Satria, Hasrul I Komang Gede Wiryawan Ici Dianita Ifah Munifah Ika Rahmatul Layly Iman Rusmana Inayah, Mazidah Noer IRA ERDIANDINI, IRA IRFAN ANWAR FAUZAN IS HELIANTI Isra Janatiningrum ISRA JANATININGRUM It Jamilah La Ode Wahidin LAKSMI AMBARSARI Lenni Fitri Lenni Fitri Lilis Nuraida Lily Nathalia Loli Natalia Lukman, Zulfiqar M. EDY SOFIYANTO Maggy T Suhartono Maggy T Suhartono Maggy Thenawidjaja Suhartono Maggy Thenawidjaja Suhartono Maggy Thenawidjaya Suhartono Mahsunah, Anis Herliyati Malikah Azizah Manguntungi, Baso Maranatha, Besty Mareistia Fassah, Dilla Maria Sugiharti Marini Wijayanti MARINI WIJAYANTI MARTIN ENGELHAUPT Mernawati, Mernawati Muhammad Nur Kholis, Muhammad Nur Muhammad Subhan Hamka MUHAMMAD ZAIRIN JUNIOR MUNTI YUHANA NANIK RAHMANI Nanik Rahmani Nanik Rahmani Naswandi Nur Natalia, Loli Nathalia, Lily NI WAYAN SRI AGUSTINI Niken Financia Gusmawati NISA RACHMANIA MUBARIK Nisfatin Shofiana NUNUK WIDHYASTUTI Nunuk Widhyastuti Nur Richana Nur, Hasrul Satria Nurfadhilla Rahmadhani Nurhasna, Aprilia Prima Agung Prihandono Puspitasari, Dian Japany RAHAYU WULAN Rahayu Wulan Rahayu Wulan RAMADHAN, ZULFA AULIA Rika Indri Astuti RIKA RAFFIUDIN Rismawati . Rizky Anggreandari RONI RIDWAN RONI RIDWAN Rosyidah, Elly RUSLI FIDRIYANTO SAFITRI NURLAELA Shanti Ratnakomala SHANTI RATNAKOMALA Sidiq, Muhammad Raffel SINTA AGUSTINA SINTA AGUSTINA SINTA AGUSTINA Sipriyadi Sipriyadi Sipriyadi Sitti Rahbiah Akram SRI ESTUNINGSIH Sri Estuningsih Sri Koerniati Sri Listiyowati Sri Suharti Stanislaus Aditya Agung SUHARSONO Sumarni Nompo Sunarti, Titi Suyono, Meisy Nawang Titi Candra Sunarti TRIO HENDARWIN TRIO HENDARWIN Trio Hendarwin Tryanisa Ridla Amalia Turnip, Enita Romasni Umi Fatmawati UMI FATMAWATI Vestika Iskawati Wahidul Hijah Wahyu Afrilasari Wahyu Widosari WENANG MAHARSIWI WIDANARNI WIDANARNI Widosari, Wahyu WILFADRI PUTRA JONESTI WULAN, RAHAYU Yantyati Widyastuti Yantyati Widyastuti YAYA RUKAYADI Yaya Rukayadi YAYA RUKAYADI Yopi - Yopi Yopi Yopi Yopi YOPI YOPI Yopi, YULIANA, META YULIN LESTARI Zahrani, Siti Mutia