Anja Meryandini
Department Of Biology, Faculty Of Mathematics And Natural Sciences, IPB University, IPB Darmaga Campus, Bogor 16680; Center For Biotechnology, International Research Institute, IPB University, IPB Darmaga Campus, Bogor 16680

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Isolation and Selection of Bacillus spp. as Candidate Probiotics for Use in Chickens Adawiah, Adilah; Wiryawan, Komang Gede; Meryandini, Anja
Makara Journal of Science Vol. 29, No. 2
Publisher : UI Scholars Hub

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Abstract

The growth rate of chickens can be improved by using probiotics as alternatives to antibiotics. Bacillus spp. as probiotics are particularly beneficial due to ability to form spores, which remain stable as commercial products over extended periods. This study aimed to isolate and select Bacillus spp. from Jasinga soil as probiotic candidates for chickens. A total of 15 isolates obtained of these, 12 were identified as Bacillus spp based on microscopic observations and screening test including ascertaining hemolysis, protease, amylase, and cellulase activities. Only six isolates exhibited gamma hemolysis and the ability to hydrolyze proteins, starch, and cellulose. Isolates BTJ1.1, 1.2, 1.4, and 2.3 poliferated in 0.3% bile salts. BTJ1.1, 1.2 and 2.3 also exhibited antimicrobial activity and sensitivity to amoxicillin, tetracycline, and rifampicin. BTJ1.1 and 2.3 were highly hydrophobic, a key probiotic trait. 16S rRNA gene sequence analysis revealed that BTJ1.1 and 2.3 were >99% similar to B. amyloliquefaciens strain B54 and B. subtilis strain ASI3, respectively, highlighting their potential as probiotics.
Viabilitas mikroba selulolitik pada media pod dan pulp kakao (Theobroma cacao L.) Nisfatin Shofiana; Titi Candra Sunarti; Anja Meryandini
Menara Perkebunan Vol. 91 No. 2 (2023): 91 (2), 2023
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v91i2.552

Abstract

Cocoa production in Indonesia is increasing from year to year. Cocoa production increased has an impact on increasing waste cocoa pod and pulp. Cocoa pod contains lignocellulose, while cocoa pulp contains many sugars. The composition of cocoa pod and pulp allows the cellulolytic microbe to grow. This study aims to perform microbial selection from fermented cocoa and sugar cane can grow in cocoa pod and pulp (Theobroma cacao). The selection method using the CMC 1%, pod 1%, pulp 1%, and pod 0,5%+pulp 0,5% media. Then, the analysis of reducing sugar using DNS method, while the total sugar analysis using phenol-H2SO4 method. Sugars of hydrolisis result were analyzed quantitatively using TLC. The results of microbial selection on CMC 1%, pod %, pulp 1%, and pod 0,5%+pulp 0,5% media obtained TBT 3.2 have the largest cellulolytic index on the pod 1% media. Isolates TBT 3.2 grown on pod 1% medium produce reducing sugar is 4,965 mg/mL at 30 hours sampling with highest total sugar at 6 hours sampling is 9,789 mg/mL. The types of sugars identified using the TLC are mannose, galactose, glucose, and selobiose.
Application of lactic acid bacteria to improve the food safety of sago starch Tryanisa Ridla Amalia; Titi Candra Sunarti; Anja Meryandini
Menara Perkebunan Vol. 92 No. 2 (2024): 92(2), 2024
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v92i2.579

Abstract

Sago starch production in local industries is still carried out traditionally and uses poor-quality water. This production causes sago starch to be fermented spontaneously, resulting in sour sago and possibly contamination by pathogenic bacteria. Lactic acid bacteria (LAB) can produce lactic acid and are suitable for use as a starter. Adding LAB as a starter in sago starch fermentation is expected to reduce the number of pathogenic bacterial growths, thereby increasing food safety in sago starch. This research aimed to obtain LAB and evaluate their use in sago starch fermentation to improve food safety. LAB selection was conducted by testing the LAB tolerance ability to low pH and the adaptability of the LAB growth in sago starch. This study was carried out using and without a LAB liquid starter. The water source during the fermentation originated from drinking water and the sago starch industrial factory. The fermentation was carried out for ten days at room temperature with an observation every two days. The results showed that fermented sago starch using drinking water did not harbor E. coli, Salmonella, or Shigella bacterial contamination. In contrast, sago starch fermented using water from the factory harbored these bacterial contaminations. Adding LAB IL1 isolate as a starter in fermentation showed the ability to reduce the number of pathogenic bacteria in sago starch.
Pemanfaatan Bakteri Selulolitik dan Xilanolitik yang Potensial untuk Dekomposisi Jerami Padi Nur, Hasrul Satria; Meryandini, Anja; Hamim, .
JOURNAL OF TROPICAL SOILS Vol. 14 No. 1: January 2009
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2009.v14i1.71-80

Abstract

There were 3 prospective isolates of cellulolytic bacteria resulted from the total of 31 isolates we found, i.e. C4-4, C5-1, and C11-1.  Four combinations of bacteria including C4-4 + Xilanolytic (A), C5-1 + Xilanolytic (B), C11-1 + Xilanolytic (C), 45I-3 + 234P-16 (D), and control (E, without bacteria) were applied as inoculant of rice straw decomposition.  In the incubation period the pH-H2O value of C4-4 + Xilanolytic (A) and C5-1 + Xilanolytic (B) was relatively stable.  The C/N ratio of all treatments decreased after 3 weeks of incubation.  The C/N ratio value of A, B, C, D and E treatments were 22.48, 23.43, 27.49, 26.82, and 29.53 respectively.  Decomposition rate all of combination treatments were faster than the control.  The content of macro-micro nutrient of A, B, C, and D treatments increased in the end of measurement, while the control didn’t.  The physical characteristic of substrate after incubation was better in A and B treatments that others.  The result indicated that the combination of C4-4 + Xilanolytic (A) and C5-1 + Xilanolytic (B) bacteria were the best combination for decomposition of rice straw. 
Antioxidant activity, total phenolic, flavonoid, and caffeine contents of robusta coffee (Coffea canephora) fermented with lactic acid bacteria Aisyah, Nadira; Sunarti, Titi Candra; Meryandini, Anja
Menara Perkebunan Vol. 93 No. 1 (2025): 93(1), 2025
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v93i1.604

Abstract

Robusta coffee is widely cultivated in Indonesia, but it struggles to dominate the global market due to its bitterness, slightly sour taste, and higher caffeine content. Coffee bean fermentation can be done to reduce undesirable characteristics in robusta coffee. This study aimed to evaluate the effects of lactic acid bacteria fermentation on the quality of coffee and its brewing characteristics, comparing it to spontaneous fermentation. The goal was to explore the potential of fermented coffee beans as functional beverages with health benefits. Three lactic acid bacteria (LAB) isolates were tested, and Lactiplantibacillus plantarum H 2.34 was identified as the most effective starter culture for coffee fermentation. After 12 hours of fermentation using coffee mucilage, LAB growth was significantly enhanced. A significant increase in flavonoid content was observed in coffee fermented with the starter culture. All brewed coffee samples demonstrated strong antioxidant activity, and LAB fermentation successfully reduced caffeine content by 4.85%. These results suggest that fermented robusta coffee could offer functional health benefits while improving its sensory profile.
EFEK PROBIOTIK Bacillus megaterium PTB 1.4 DAN Pediococcus pentosaceus E2211 TERHADAP REPONS IMUN DAN KELANGSUNGAN HIDUP IKAN LELE (Clarias sp.) SELAMA UJI TANTANG Aeromonas hydrophila Hamka, Muhammad Subhan; Meryandini, Anja; Widanarni, Widanarni; Kurniaji, Ardana
JFMR (Journal of Fisheries and Marine Research) Vol. 5 No. 3 (2021): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2021.005.03.9

Abstract

Penyakit bakterial pada kegiatan budidaya ikan sudah menjadi masalah yang sering dihadapi pembudidaya. Salah satu jenis bakteri yang menyebabkan penyakit pada ikan lele (Clarias sp.) adalah bakteri Aeromonas hydrophila. Tujuan penelitian ini untuk mengevaluasi efek pemberian probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 terhadap respons imun dan kelangsungan hidup ikan lele selama uji tantang dengan bakteri A. hyrophila. Probiotik yang digunakan adalah kombinasi 0,5% B. megaterium PTB 1.4 dan 0,5%  P. pentosaceus E2211. Pemberian probiotik dilakukan pada hari ke-0 hingga hari ke-30 melalui pakan. Uji tantang dilakukan pada hari ke-30 hingga hari ke-40. Hasil penelitian menunjukkan bahwa pemberian probiotik dapat berpengaruh terhadap respons imun dan kelangsungan hidup ikan lele dalam menekan infeksi A. hydrophila. Total eritrosit, hemoglobin, hematokrit, total leukosit, aktivitas fagositik dan aktivitas lisozim signifikan (P<0,05) teramati meningkat pada hari ke-35 dan menurun pada hari ke-40. Kelangsungan hidup ikan lele berhasil dipertahankan pada 87,62±4,83%. Total bakteri A. hydrophila meningkat pada hari ke-35 dan menurun pada hari ke-40. Pemberian probiotik B. megaterium PTB 1.4 dan P. pentosaceus E2211 pada dosis 0,5% dapat menekan pertumbuhan bakteri A. hydrophila dan meningkatkan status kesehatan ikan lele.
In silico characterization of adh1 gene encoding alcohol dehydrogenase 1 (ADH1) from non-conventional yeast, Wickerhamomyces and Pichia spp HARTONO, FAISAL DINIAMAL; MERYANDINI, ANJA; ASTUTI, RIKA INDRI
Jurnal Natural Volume 25 Number 3, October 2025
Publisher : Universitas Syiah Kuala

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Abstract

Wickerhamomyces anomalus and Pichia kudriavzevii have high potential to produce bioethanol under high stress condition, due to their stress-tolerant properties. To elucidate and develop an efficient and sustainable bioethanol production, characterization of ethanol fermentation reactions is highly substantial. Ethanol fermentation employs key enzyme ADH1 encoded by ADH1 gene, important for conversion of acetaldehyde to ethanol. However, structural studies about alcohol dehydrogenase1 from these genera of yeasts are limited. This study aimed to detect the alcohol dehydrogenase 1 gene from Pichia spp. Using computational-bioinformatics approaches. The adh1 gene was amplified by PCR, visualized by electrophoresis, and analysed for sequence homology by BlastN and BlastP. The enzyme structure was constructed by SWISS-MODEL and I-TASSER with validation by Ramachandran plot, QMEAN4, and Local Quality Estimate. The Similarity and homology analysis of ADH1 genes and their corresponding protein sequence of yeast isolates showed that the ADH1gene was successfully detected. Multiple sequence alignment (MSA) and phylogenetic tree revealed that W. anomalus BT1-BT6 has close evolutionary relationship with ADH1 from Saccharomyces cerevisiae sequence while P. kudriavzevii IP4 showed different pattern. The ADH 1 enzyme model, generated using the SWISS-MODEL web server, demonstrated the best stereochemical quality, with a Ramachandran plot value of 100% for W. anomalus BT1 and 99.3% for P. kudriavzevii IP4. Superimposition of 3D-predicted model of ADH1 from W. anomalus BT1 and P. kudriavzevii 1P4 showed an exact match with amino acid in Zn2+ binding sites, confirming the ADH1 metaloenzyme properties. These findings provide structural insights about ADH1 genes and protein properties which can be used further for the development of efficient and high productivity of bioethanol productions through genetic and protein engineering.
Producing Lactobacillus plantarum Dry Starter using Rice Bran Matrix Nurhasna, Aprilia; Meryandini, Anja; Candra Sunarti , Titi
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/10654

Abstract

Background: Lactic Acid Bacteria (LAB) starter is an instant LAB culture that has been prepared to initiate lactic acid production in the fermentation process. The manufacture of BAL starter requires alternative media as a substitute for MRS media which costs a lot when used for industrial production. To protect cells, an encapsulant matrix is needed which functions to provide insulation for bacterial cells against the drying process which can damage cells and a good media is needed and is capable of being an encapsulation matrix in the manufacture of dry LAB starter. Lactobacillus plantarum NHC6 bacteria is a lactic acid bacteria that has the potential to be developed. Method: making encapsulation matrix using rice bran. The bran media consisted of 10% (w/v) rice bran added with 5% (w/v) glucose and 1% (w/v) ammonium sulfate. As much as 10% (w/v) of L. plantarum NHC6 culture which was in the late log phase was inoculated in bran media. The liquid starter was then incubated again for 14 hours at 37°C. After that, the drying process was carried out using a spray dryer at an inlet temperature of 170°C. Result: The number of live cells in the bran starter decreased after the spray drying process. Starter storage at 28°C and 4°C has a significantly different effect. Conclusion: Rice bran matrix is capable of being an encapsulating agent and is able to protect L. plantarum NHC6 cells from high temperatures during the spray drying method in the manufacture of dry starter.
A CONTROL OF OXYGEN SUPPLY IN BIOCONVERSION OF SUGARCANE TRASH INTO XYLITOL BY Meyerozyma guilliermondii InaCCY65 Thontowi, Ahmad; Kanti, Atit; Listiyowati, Sri; Meryandini, Anja
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.7720

Abstract

Xylitol is a sugar alcohol used as a sugar substitute for several prevention of health cases such as dental diseases, diabetes, and other health problems. Bioconversion of xylose into xylitol needs an optimum oxygen supply for xylitol synthesis. This research aims to determine the effect of dissolved oxygen on hydrolysate fermentation from sugar cane leaf as a source of xylose to xylitol by Meyerozyma guilliermondii InaCCY65. Dissolved oxygen was varied in aeration and fermentation agitation using a 3L scale bioreactor. Analysis of cell growth for several kinetic parameters during fermentation, xylose reductase, and xylitol dehydrogenase activity. Bioconversion of sugarcane trash hydrolysate into xylitol by M. guilliermondii InaCCY65 is influenced by the oxygen transfer coefficient (kLa) and aeration conditions. The increase in kLa number showed increased cell growth, xylose consumption, xylitol production, and decreased InaCCY65 cells. The optimum conditions of kLa were obtained at 45/h with 39 g/L xylitol production under the aeration effect. Optimum aeration in the bioconversion of sugarcane trash (SCT) hydrolysate become xylitol by M. guilliermondii InaCCY65 is 1.0%; under these conditions, xylitol yield and xylitol productivity are 0.78 g/g and 1.20 g/Lh. The effect of oxygen in the bioconversion of xylose to xylitol also has an impact on xylose reductase (XR) and xylose dehydrogenase (XDH) activities of M. guiellermondii InaCCY65. The results showed that the dissolved oxygen concentration must be carefully controlled during xylitol bioconversion to obtain efficient xylitol.
PENGEMBANGAN GALUR Pichia kudriavzevii TOLERAN CEKAMAN OSMOTIK GLUKOSA MELALUI EVOLUSI LABORATORIUM ADAPTIF Sidiq, Muhammad Raffel; Astuti, Rika Indri; Meryandini, Anja
Berita Biologi Vol 24 No 2 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.9025

Abstract

Bioetanol merupakan salah satu komoditas energi terbarukan terbesar di dunia di samping biodiesel. Efisiensi proses fermentasi etanol dapat dilakukan melalui penggunaan khamir fermentatif yang toleran cekaman fermentasi di antaranya cekaman osmotik yang dapat terjadi akibat penggunaan konsentrasi glukosa tinggi sebagai substrat fermentasi. Penelitian ini bertujuan untuk mengkonstruksi khamir mutan Pichia kudriavzevii galur RWT yang toleran cekaman osmotik glukosa melalui teknik evolusi laboratorium adaptif. Khamir P. kudriavzevii toleran cekaman omostik glukosa 35% berhasil diperoleh dan diberi kode isolat P. kudriavzevii RM. Namun,  P. kudriavzevii RM tumbuh optimum pada kadar glukosa 20% dan tidak memiliki perbedaan morfologi dengan isolat tipe liarnya (RWT). Isolat khamir mutan memiliki fase log yang lebih lama dibandingkan isolat tipe liarnya (RWT) dan khamir etanologenik Saccharomyces cerevisiae. Hal ini mengindikasikan potensinya sebagai agen fermentasi bioetanol, karena bioetanol diproduksi pada fase pertumbuhan ini. Melalui pengamatan aktivitas mitokondria diketahui terjadi pergeseran sistem metabolisme dari respirasi aerobik menjadi fermentatif terlihat pada khamir P. kudriavzevii mutan ketika ditumbuhkan pada medium dengan kadar glukosa 20%. Penelitian lebih lanjut terkait produktivitas khamir mutan P. kudriavzevii mutan osmotoleran dalam memproduksi etanol perlu dilakukan untuk pemanfaatannya sebagai agen fermentasi.
Co-Authors . Hamim . KURRATAA’YUN . YOPI . YOPI ., Yopi ., Yopi A, Gading Wilda A, Gading Wilda Abd. Rasyid Syamsuri Abdjad Asih Nawangsih Adawiah, Adilah Ade Andriani ADE ANDRIANI Ade Irma Ahmad Thontowi Aisyah, Nadira Alfred Michael ALINA AKHDIYA Alina Akhdiya AMOR TRESNA KARYAWATI Amor Tresna Karyawati, Amor Tresna Andika Susantri ANGGRAINI PUTRI UTAMI Anggreandari, Rizky Antonius Suwanto Anwar, Irvan Apon Zaenal Mustopa APRILIA NAOMI Ardana Kurniaji Ariandi Arina Amalia Putri Aris Tri Wahyudi Ariyanto, Yogy Satria Armita, Dea Asrianti Basri Atit Kanti Azizah Hikma Safitri Bambang Prasetya Bambang Prasetya Basri, Asrianti Basri, Asrianti Bedah Rupaedah Besty Maranatha CAHYA PRIHATNA Candra Sunarti , Titi Cocok Ana Maryani Berutu Cynthia Rizka Riani DAROJATUL ULYA Dede Heri Yuli Yanto Deden Saprudin Dedy Duryadi Solihin DEDY DURYADI SOLIHIN Deford Cristy Birahy DERI YURATMOKO DEWI APRI ASTUTI Dewi Asnita Dewi, Fitria Diana, Milsa Solva DIMAS ANDRIANTO DINI NURDIANI Dwi Ambarawati Dwi Andreas Santosa DWI SURYANTO DWI SURYANTO Dyah Iswantini Eka Putri EKA RURIANI Elly Rosyidah Engelhaupt, Martin Esti Utarti FAHRRUROZI FAHRRUROZI Fahrurrozi Fahrurrozi FAHRURROZI FAHRURROZI Fahrurrozi Fahrurrozi Fathiah, Muhammad Fadhil Fathin Hamida Faturrahman Faturrahman FATURRAHMAN FATURRAHMAN Ferry Mutia FERY MUTIA Fitri, Ainissya Fitria Dewi Glisina Dwinoor Rembulan GRISELDA HERMAN NATADIPUTRI Hairani, Atikah Hamim Hamim Hamtini - Hamtini Hanni Tsaaqifah Hari Eko Irianto Hartono, Faisal Diniamal Hasrul Satria Hasrul Satria Nur Hasrul Satria, Hasrul I Komang Gede Wiryawan Ici Dianita Ifah Munifah Ika Rahmatul Layly Iman Rusmana Inayah, Mazidah Noer IRA ERDIANDINI, IRA IRFAN ANWAR FAUZAN IS HELIANTI Isra Janatiningrum ISRA JANATININGRUM It Jamilah La Ode Wahidin LAKSMI AMBARSARI Lenni Fitri Lenni Fitri Lilis Nuraida Lily Nathalia Loli Natalia Lukman, Zulfiqar M. EDY SOFIYANTO Maggy T Suhartono Maggy T Suhartono Maggy Thenawidjaja Suhartono Maggy Thenawidjaja Suhartono Maggy Thenawidjaya Suhartono Mahsunah, Anis Herliyati Malikah Azizah Manguntungi, Baso Maranatha, Besty Mareistia Fassah, Dilla Maria Sugiharti MARINI WIJAYANTI Marini Wijayanti MARTIN ENGELHAUPT Mernawati, Mernawati Muhammad Nur Kholis, Muhammad Nur Muhammad Subhan Hamka MUHAMMAD ZAIRIN JUNIOR MUNTI YUHANA NANIK RAHMANI Nanik Rahmani Nanik Rahmani Naswandi Nur Natalia, Loli Nathalia, Lily NI WAYAN SRI AGUSTINI Niken Financia Gusmawati NISA RACHMANIA MUBARIK Nisfatin Shofiana NUNUK WIDHYASTUTI Nunuk Widhyastuti Nur Richana Nur, Hasrul Satria Nurfadhilla Rahmadhani Nurhasna, Aprilia Prima Agung Prihandono Puspitasari, Dian Japany Rahayu Wulan RAHAYU WULAN Rahayu Wulan RAMADHAN, ZULFA AULIA Rika Indri Astuti RIKA RAFFIUDIN Rismawati . Rizky Anggreandari RONI RIDWAN RONI RIDWAN Rosyidah, Elly RUSLI FIDRIYANTO SAFITRI NURLAELA SHANTI RATNAKOMALA Shanti Ratnakomala Sidiq, Muhammad Raffel SINTA AGUSTINA SINTA AGUSTINA SINTA AGUSTINA Sipriyadi Sipriyadi Sipriyadi Sitti Rahbiah Akram SRI ESTUNINGSIH Sri Estuningsih Sri Koerniati Sri Listiyowati Sri Suharti Stanislaus Aditya Agung SUHARSONO Sumarni Nompo Sunarti, Titi Suyono, Meisy Nawang Titi Candra Sunarti TRIO HENDARWIN Trio Hendarwin TRIO HENDARWIN Tryanisa Ridla Amalia Turnip, Enita Romasni UMI FATMAWATI Umi Fatmawati Vestika Iskawati Wahidul Hijah Wahyu Afrilasari Wahyu Widosari WENANG MAHARSIWI WIDANARNI WIDANARNI Widosari, Wahyu WILFADRI PUTRA JONESTI WULAN, RAHAYU Yantyati Widyastuti Yantyati Widyastuti Yaya Rukayadi YAYA RUKAYADI YAYA RUKAYADI Yopi - Yopi Yopi YOPI YOPI Yopi Yopi Yopi, YULIANA, META YULIN LESTARI Zahrani, Siti Mutia