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In silico characterization of adh1 gene encoding alcohol dehydrogenase 1 (ADH1) from non-conventional yeast, Wickerhamomyces and Pichia spp HARTONO, FAISAL DINIAMAL; MERYANDINI, ANJA; ASTUTI, RIKA INDRI
Jurnal Natural Volume 25 Number 3, October 2025
Publisher : Universitas Syiah Kuala

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Abstract

Wickerhamomyces anomalus and Pichia kudriavzevii have high potential to produce bioethanol under high stress condition, due to their stress-tolerant properties. To elucidate and develop an efficient and sustainable bioethanol production, characterization of ethanol fermentation reactions is highly substantial. Ethanol fermentation employs key enzyme ADH1 encoded by ADH1 gene, important for conversion of acetaldehyde to ethanol. However, structural studies about alcohol dehydrogenase1 from these genera of yeasts are limited. This study aimed to detect the alcohol dehydrogenase 1 gene from Pichia spp. Using computational-bioinformatics approaches. The adh1 gene was amplified by PCR, visualized by electrophoresis, and analysed for sequence homology by BlastN and BlastP. The enzyme structure was constructed by SWISS-MODEL and I-TASSER with validation by Ramachandran plot, QMEAN4, and Local Quality Estimate. The Similarity and homology analysis of ADH1 genes and their corresponding protein sequence of yeast isolates showed that the ADH1gene was successfully detected. Multiple sequence alignment (MSA) and phylogenetic tree revealed that W. anomalus BT1-BT6 has close evolutionary relationship with ADH1 from Saccharomyces cerevisiae sequence while P. kudriavzevii IP4 showed different pattern. The ADH 1 enzyme model, generated using the SWISS-MODEL web server, demonstrated the best stereochemical quality, with a Ramachandran plot value of 100% for W. anomalus BT1 and 99.3% for P. kudriavzevii IP4. Superimposition of 3D-predicted model of ADH1 from W. anomalus BT1 and P. kudriavzevii 1P4 showed an exact match with amino acid in Zn2+ binding sites, confirming the ADH1 metaloenzyme properties. These findings provide structural insights about ADH1 genes and protein properties which can be used further for the development of efficient and high productivity of bioethanol productions through genetic and protein engineering.
Producing Lactobacillus plantarum Dry Starter using Rice Bran Matrix Nurhasna, Aprilia; Meryandini, Anja; Candra Sunarti , Titi
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/10654

Abstract

Background: Lactic Acid Bacteria (LAB) starter is an instant LAB culture that has been prepared to initiate lactic acid production in the fermentation process. The manufacture of BAL starter requires alternative media as a substitute for MRS media which costs a lot when used for industrial production. To protect cells, an encapsulant matrix is needed which functions to provide insulation for bacterial cells against the drying process which can damage cells and a good media is needed and is capable of being an encapsulation matrix in the manufacture of dry LAB starter. Lactobacillus plantarum NHC6 bacteria is a lactic acid bacteria that has the potential to be developed. Method: making encapsulation matrix using rice bran. The bran media consisted of 10% (w/v) rice bran added with 5% (w/v) glucose and 1% (w/v) ammonium sulfate. As much as 10% (w/v) of L. plantarum NHC6 culture which was in the late log phase was inoculated in bran media. The liquid starter was then incubated again for 14 hours at 37°C. After that, the drying process was carried out using a spray dryer at an inlet temperature of 170°C. Result: The number of live cells in the bran starter decreased after the spray drying process. Starter storage at 28°C and 4°C has a significantly different effect. Conclusion: Rice bran matrix is capable of being an encapsulating agent and is able to protect L. plantarum NHC6 cells from high temperatures during the spray drying method in the manufacture of dry starter.
USING STREPTOMYCES XYLANASE TO PRODUCE XYLOOLIGOSACHARIDE FROM CORNCOB MERYANDINI, ANJA; SUNARTI, TITI CANDRA; NAOMI, APRILIA; MUTIA, FERY
BIOTROPIA Vol. 15 No. 2 (2008): BIOTROPIA Vol. 15 No. 2 December 2008
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2008.15.2.71

Abstract

Streptomyces 234P-16 and SKK1-8 are xylanase-producing bacteria. Corncob xylan were extracted using acidified method. Crude enzymes (produced by centrifuging the culture) were used to hydrolyze xylan from 2 varieties of corncob. Crude extract activity was measured by using DNS (Dinitrosalisilic Acid) method. Xylanase from strain 234P-16 has the highest activity if cultivated in 1% Hawaii xylan, whereas strain SKK1-8 on 1.5% Bisma xylan. SKK1-8 xylanase can hydrolize corncob xylan (1% Hawaii or 1.5% Bisma xylan) within 4 hours and produce xylooligosacharide with polymerization degree of 4.76 and 6.37, respectively. Key words: Xylanase, Xylooligosacharide, Streptomyces.
SACCHARIFICATION OF CORNCOB USING CELLULOLYTIC BACTERIA FOR BIOETHANOL PRODUCTION SUNARTI, TITI CANDRA; MERYANDINI, ANJA; SOFIYANTO, M. EDY; RICHANA, NUR
BIOTROPIA Vol. 17 No. 2 (2010): BIOTROPIA Vol. 17 No. 2 December 2010
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8621.122 KB) | DOI: 10.11598/btb.2010.17.2.80

Abstract

The use of cellulose degrading enzyme (cellulases) for hydrolysis of lignocellulosic material is a part of bioethanol production process.  In this experiment,  delignified corncob,  its cellulose fraction and alpha cellulose were used as substrates to produce fermentable sugar by using three local isolates of celluloytic bacteria (C5-1, C4-4, C11-1 and Cmix ; mixed cultures of three isolates), and Saccharomyces cereviseae to produce ethanol. The results showed that all isolates of cellulolytic bacteria can grow on cellulose fraction better than on  delignified corncob, and alpha cellulose.  The highest hydrolytic activity produced from cellulose fraction was by isolate C4-4, which liberated 3.50 g/l of total sugar.  Ethanol can be produced by mixed culture of bacteria and yeast, but because of competitive growth, the fermentation only produced 0.39-0.47 g/l of ethanol. Keywords:  Celluloses, delignified corncob
Pectinase Production and Clarification Treatments of Apple (Malus Domestica) Juice Berutu, Cocok Ana Maryani; Fahrurrozi, Fahrurrozi; Meryandini, Anja
Annales Bogorienses Vol. 21 No. 2 (2017): Annales Bogorienses
Publisher : BRIN

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Abstract

Pectinases are a group of an enzyme that break down pectin, a polysaccharide that is found in plant cell walls. Today, the application of pectinolytic enzymes plays an important role in food technology for the maceration of fruits and vegetables, including for the extraction and clarification of juice. This research aimed to produce pectinase enzyme for clarifying of apple juice. A microbial culture was selected from cocoa bean fermentation samples and identified as Bacillus sp.. The highest enzyme activity was investigated after 48 hours of incubation. Citrus pectin as the carbon source and peptone as the nitrogen source was found as the best component for pectinase production. The optimum condition of pectinase activity was observed at pH 5, temperature 40 °C and the crude enzyme had the higher activity at one hour storage. Apple juice was treated with the enzyme at different concentrations (0%, 0.5%, 1%, 2%, 4%). Apple juice clarification was evaluated for its percent clarity and viscosity. The result showed that enzyme treatment at 4% in apple juice promoted juice clarification and decreased pH and viscosity. In conclusion, the quality of apple juice can be improved by enzymatic treatment using pectinase.
Optimization of Culture Conditions for Production of β-Mannanase by Strain Nonomuraea sp. ID06-379 using Submerged Substrate Fermentation Ratnakomala, Shanti; Yopi, Yopi; Suhartono, Maggy Thenawidjaja; Meryandini, Anja; Prasetya, Bambang
Annales Bogorienses Vol. 18 No. 2 (2014): Annales Bogorienses
Publisher : BRIN

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Abstract

The objective of this study was to investigate the effect of media compositions on the production of β-mannanase by Nonomuraea sp. ID06-379. The study was focused on the influence of carbon, nitrogen, phosphorus and detergents on β-mannanase synthesis through manipulating media compositions on production medium. The results indicated that for carbon sources, locus bean gum (0.745 ± 0.036 U/ml) showed maximum mannanase activity. Malt extract was the best nitrogen source for producing β-mannanase (1.075 ± 0.006 U/ml), (NH4)2HPO4 as phosphate source (1.733 ± 0.026 U/ml) and Tween 80 (1.145 ± 0.003 U/ml) as surfactants effect on increasing permeability of bacterial cell membrane, enhancing membrane transport and excretion of extracellular enzymes into the production media. The results showed that 1% malt extract, 0.5% locus bean gum and 0.05% (NH4)2HPO4 were good substances for nitrogen source, carbon source and phosphate respectively. The highest production of β-mannanase by Nonomuraea sp. ID06-379 (5.33 U/mg) was reached in the medium optimization (Vogel’s minimal medium) contained the following ingredients: 0.5% locus bean gum, 1% malt extract and 0.05% (NH4)2HPO4, under submerged fermentation with shaking at 120 rpm and 28C for 2 days incubation.
A CONTROL OF OXYGEN SUPPLY IN BIOCONVERSION OF SUGARCANE TRASH INTO XYLITOL BY Meyerozyma guilliermondii InaCCY65 Thontowi, Ahmad; Kanti, Atit; Listiyowati, Sri; Meryandini, Anja
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.7720

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Xylitol is a sugar alcohol used as a sugar substitute for several prevention of health cases such as dental diseases, diabetes, and other health problems. Bioconversion of xylose into xylitol needs an optimum oxygen supply for xylitol synthesis. This research aims to determine the effect of dissolved oxygen on hydrolysate fermentation from sugar cane leaf as a source of xylose to xylitol by Meyerozyma guilliermondii InaCCY65. Dissolved oxygen was varied in aeration and fermentation agitation using a 3L scale bioreactor. Analysis of cell growth for several kinetic parameters during fermentation, xylose reductase, and xylitol dehydrogenase activity. Bioconversion of sugarcane trash hydrolysate into xylitol by M. guilliermondii InaCCY65 is influenced by the oxygen transfer coefficient (kLa) and aeration conditions. The increase in kLa number showed increased cell growth, xylose consumption, xylitol production, and decreased InaCCY65 cells. The optimum conditions of kLa were obtained at 45/h with 39 g/L xylitol production under the aeration effect. Optimum aeration in the bioconversion of sugarcane trash (SCT) hydrolysate become xylitol by M. guilliermondii InaCCY65 is 1.0%; under these conditions, xylitol yield and xylitol productivity are 0.78 g/g and 1.20 g/Lh. The effect of oxygen in the bioconversion of xylose to xylitol also has an impact on xylose reductase (XR) and xylose dehydrogenase (XDH) activities of M. guiellermondii InaCCY65. The results showed that the dissolved oxygen concentration must be carefully controlled during xylitol bioconversion to obtain efficient xylitol.
PENGEMBANGAN GALUR Pichia kudriavzevii TOLERAN CEKAMAN OSMOTIK GLUKOSA MELALUI EVOLUSI LABORATORIUM ADAPTIF Sidiq, Muhammad Raffel; Astuti, Rika Indri; Meryandini, Anja
Berita Biologi Vol 24 No 2 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.9025

Abstract

Bioetanol merupakan salah satu komoditas energi terbarukan terbesar di dunia di samping biodiesel. Efisiensi proses fermentasi etanol dapat dilakukan melalui penggunaan khamir fermentatif yang toleran cekaman fermentasi di antaranya cekaman osmotik yang dapat terjadi akibat penggunaan konsentrasi glukosa tinggi sebagai substrat fermentasi. Penelitian ini bertujuan untuk mengkonstruksi khamir mutan Pichia kudriavzevii galur RWT yang toleran cekaman osmotik glukosa melalui teknik evolusi laboratorium adaptif. Khamir P. kudriavzevii toleran cekaman omostik glukosa 35% berhasil diperoleh dan diberi kode isolat P. kudriavzevii RM. Namun,  P. kudriavzevii RM tumbuh optimum pada kadar glukosa 20% dan tidak memiliki perbedaan morfologi dengan isolat tipe liarnya (RWT). Isolat khamir mutan memiliki fase log yang lebih lama dibandingkan isolat tipe liarnya (RWT) dan khamir etanologenik Saccharomyces cerevisiae. Hal ini mengindikasikan potensinya sebagai agen fermentasi bioetanol, karena bioetanol diproduksi pada fase pertumbuhan ini. Melalui pengamatan aktivitas mitokondria diketahui terjadi pergeseran sistem metabolisme dari respirasi aerobik menjadi fermentatif terlihat pada khamir P. kudriavzevii mutan ketika ditumbuhkan pada medium dengan kadar glukosa 20%. Penelitian lebih lanjut terkait produktivitas khamir mutan P. kudriavzevii mutan osmotoleran dalam memproduksi etanol perlu dilakukan untuk pemanfaatannya sebagai agen fermentasi.
Application of Lactic Acid Bacteria to Produce Bioactive Compounds from Tofu Waste Using Pineapple Bromelain Enzyme Lukman, Zulfiqar; Andrianto, Dimas; Meryandini, Anja
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.445-454

Abstract

Tofu is one of the main human foodstuffs and has become an alternative substitute for animal protein. Tofu contains relatively high protein, and active compounds, but the waste produced during the tofu processing process is very abundant, namely 3.5 million tons per year in Indonesia. The production of active compounds from tofu waste can use bromelain enzymes. The bromelain enzyme in this study was produced from the fermentation of pineapple leaf waste using Pediococcus pentosaceus E7. The production of active compounds from tofu waste has not been widely reported, so this study aimed to produce active compounds from tofu waste. This study began with bromelain production, measurement of bromelain activity, bromelain precipitation, peptide production from tofu waste, Liquid Chromatography-Mass Spectrometry (LC-MS) analysis, and antibacterial testing of active compounds derived from tofu waste degradation. The results showed that the peptide content of tofu liquid waste degraded using the bromelain enzyme was 41.01±0.06 mg/mL. The LCMS results showed that 18 bioactive compounds were contained in the degradation products of tofu liquid waste. The active compounds derived from the degradation of tofu waste are capable of inhibiting the growth of Salmonella Typhimurium ATCC 14028, yielding a clear zone index of 2.53±0.12.
CHARACTERIZATION OF STREPTOMYCES sp. 45I-3 XYLANASE Meryandini, Anja; Saprudin, Deden; Prihandono, Prima Agung; Akhdiya, Alina; Hendarwin, Trio
BIOTROPIA Vol. 14 No. 2 (2007): BIOTROPIA Vol. 14 No. 2 December 2007
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (626.57 KB) | DOI: 10.11598/btb.2007.14.2.16

Abstract

Streptomyces sp 45I-3 producing xylanase was isolated from Kalimantan soil sample. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase has an optimum condition at pH 5 and 50 oC.  Crude xylanase have half-life time of 2 hours 9 minute at 50 oC, whereas purified xylanase has half-life time of 12 hours. The molecular mass of purified xylanase was determined to be 43.2 and 39.2 kDa.
Co-Authors . Hamim . KURRATAA’YUN . YOPI . YOPI ., Yopi ., Yopi A, Gading Wilda A, Gading Wilda Abd. Rasyid Syamsuri Abdjad Asih Nawangsih Adawiah, Adilah Ade Andriani ADE ANDRIANI Ade Irma Ahmad Thontowi Aisyah, Nadira Alfred Michael ALINA AKHDIYA Amor Tresna Karyawati, Amor Tresna Andika Susantri Anggreandari, Rizky Antonius Suwanto Apon Zaenal Mustopa Ardana Kurniaji Ariandi Arina Amalia Putri Aris Tri Wahyudi Ariyanto, Yogy Satria Armita, Dea Asrianti Basri Atit Kanti Azizah Hikma Safitri Azizah, Malikah Bambang Prasetya Basri, Asrianti Basri, Asrianti Bedah Rupaedah Berutu, Cocok Ana Maryani Berutu, Cocok Ana Maryani Besty Maranatha Birahy, Deford Cristy Candra Sunarti , Titi Cocok Ana Maryani Berutu Cynthia Rizka Riani DAROJATUL ULYA Deden Saprudin DERI YURATMOKO DEWI APRI ASTUTI Dewi, Fitria DIMAS ANDRIANTO DINI NURDIANI Dwi Ambarawati Dyah Iswantini EKA RURIANI Elly Rosyidah Engelhaupt, Martin Esti Utarti Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Fathiah, Muhammad Fadhil Fathin Hamida Ferry Mutia Fitria Dewi Glisina Dwinoor Rembulan Hairani, Atikah Hamim Hamim Hamtini - Hamtini Hanni Tsaaqifah Hari Eko Irianto Hartono, Faisal Diniamal Hasrul Satria Hasrul Satria Nur Hasrul Satria, Hasrul I Komang Gede Wiryawan Ifah Munifah Iman Rusmana Inayah, Mazidah Noer IRA ERDIANDINI, IRA It Jamilah LAKSMI AMBARSARI Lenni Fitri Lenni Fitri Lilis Nuraida Lily Nathalia Loli Natalia Lukman, Zulfiqar Maggy T Suhartono Maggy Thenawidjaja Suhartono Maggy Thenawidjaya Suhartono Mahsunah, Anis Herliyati Manguntungi, Baso Maranatha, Besty Mareistia Fassah, Dilla Maria Sugiharti Marini Wijayanti Muhammad Nur Kholis, Muhammad Nur Muhammad Subhan Hamka MUNTI YUHANA MUTIA, FERY NANIK RAHMANI Nanik Rahmani Nanik Rahmani NAOMI, APRILIA Natalia, Loli Nathalia, Lily Niken Financia Gusmawati NISA RACHMANIA MUBARIK NUNUK WIDHYASTUTI Nunuk Widhyastuti Nur Richana Nur, Hasrul Satria Nurfadhilla Rahmadhani Nurhasna, Aprilia Prihandono, Prima Agung Puspitasari, Dian Japany Rahayu Wulan RAMADHAN, ZULFA AULIA Rika Indri Astuti Rizky Anggreandari Rosyidah, Elly SAFITRI NURLAELA SHANTI RATNAKOMALA Sidiq, Muhammad Raffel Sipriyadi Sitti Rahbiah Akram SOFIYANTO, M. EDY Sri Estuningsih Sri Koerniati Sri Listiyowati SUHARSONO Sumarni Nompo Sunarti, Titi Suyono, Meisy Nawang Titi Candra Sunarti TRIO HENDARWIN Turnip, Enita Romasni Umi Fatmawati Vestika Iskawati Wahidul Hijah Wahyu Afrilasari Wahyu Widosari WIDANARNI WIDANARNI Widosari, Wahyu WULAN, RAHAYU Yantyati Widyastuti Yaya Rukayadi Yopi - Yopi Yopi YOPI YOPI Yopi, YULIANA, META YULIN LESTARI Zahrani, Siti Mutia