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All Journal E-Jurnal Medika Udayana Bulletin of Electrical Engineering and Informatics Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Urology Jurnal Berkala Epidemiologi Jurnal Biosains Pascasarjana Jurnal NERS Indonesian Journal of Tropical and Infectious Disease Journal of Information Systems Engineering and Business Intelligence Journal of Tropical Biodiversity and Biotechnology Jurnal Kimia Riset The Indonesian Biomedical Journal Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Manajemen Kesehatan Yayasan RS.Dr. Soetomo Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Jurnal Samudra Ekonomi dan Bisnis INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Narra J Berkala Ilmiah Kimia Farmasi Hang Tuah Medical Journal International Journal of Economics (IJEC)

Aryati Aryati

Dengue Study Group, Institute Of Tropical Disease, Universitas Airlangga, Mulyorejo Street, Mulyorejo 60115, Surabaya, East Java, Indonesia Clinical Pathology Department, Faculty Of Medicine, Universitas Airlangga, Prof.Dr.Moestopo Street, Tambaksari

Author-ID : 234508

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

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Daya hambat kombinasi madu mangga (Mangifera indica)-susu probiotik terhadap pertumbuhan Eschericia coli ATCC 6538 dan Staphylococcus aureus ATCC 8739 Andyanita Hanif Hermawati; Aryati Aryati; Isnaeni Yudi Haryanto
Pharmaciana Vol 6, No 2 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Growth inhibitory activity of combination of mango honey-probiotic milk produced by Lactobacillus acidophilus (L.a) and Lactobacillus casei (L.c) against E. coli and S. aureus has been studied. The fermented milk was prepared by inoculating each probioticin milk at 43ÂºC and then incubated at room temperature for 24 hours. Diffusion agar technique by using nutrient agar media was used to assess the minimum inhibition concentration (MIC). Result of the research showed that pH of fermentation broth of the probiotic milk reached 5. Biomass obtained that calculated by Total Plate Count of L.a and L.c was 1.3 x 107 cfu/mL and 20.7 x 1013 cfu/mL respectively. Inhibition zone diameter ofthe mangohoney-L.c showed an optimum antimicrobial effect against tested bacteria at 1:9 ratio. The MIC value against E. coli and S. Aureus was 15.7 Â± 6,1 mm and 13.3 Â± 3.0 mm respectively. The activity of mango honey-L. aexhibite dan optimum antimicrobial effect against tested bacteria at 2:8.The MIC value againstE. coli and S. Aureuswas14,7 Â± 1,1mm and 13.3 Â± 1.3mm respectively.

Detection of Knockdown-Resistance Mutations (V1016G and F1534C) in Dengue Vector from Urban Park, Surabaya, Indonesia Shifa Fauziyah; Sri Subekti; Budi Utomo; Teguh Hari Sucipto; Hebert Adrianto; Aryati Aryati; Puspa Wardhani; Soegeng Soegijanto
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 3 (2021): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.65357

An urban park is potentially a source of vector-borne disease transmission due to it being a natural and artificial mosquito breeding habitats combined with people's continuous presence. Thus, this study aims to screen the occurrence of knockdown-resistance (kdr) mutant alleles (V1016G and F1534C) in mosquito populations collected from urban parks in Surabaya, Indonesia. Cross sectional study was conducted in July 2019. A total of 28 ovitraps were installed in seven urban parks, having four ovitraps installed in each park. In total, 1,662 eggs were collected, and only 187 emerged into adult mosquitoes, consisting of 97 Aedes (Stegomyia) aegypti and 90 Aedes (Stegomyia) albopictus. All-female adult mosquitoes (n=55) were tested using allele-specific polymerase chain reaction assay (AS-PCR) to detect voltage gated sodium channel (VGSC) gene mutations. This study found no mutations in Valine to Glysine mutation in point 1016 (V1016G) and Phenylalanine to Cysteine in point 1534 (F1534C) alleles in both two species. All of mosquito samples have wild type genotype of kdr alleles (V1016V and F1534F). Data were analysed using R Studio 1.4 Version by Genetics package. Results showed that the frequency of resistant alleles (G1016 and C1534) was zero, and the frequency of susceptible allele was 1 (V1016 and F1534). Insecticide bioassay could not be established due to the limited number of adult mosquitoes, so insecticide resistance status could not be determined. However, this study can be used as preliminary monitoring for the vector control program.

RNA Isolation of Dengue Virus Type 2 with Different Precipitation Solvents : Methanol, Chloroform, and 2-Isopropanol. Yovilianda Maulitiva Untoro; Teguh Hari Sucipto; Harsasi Setyawati; Siti Churrotin; Ilham Harlan Amarullah; Puspa Wardhani; Aryati Aryati; Shuhai Ueda; Soegeng Soegijanto
Jurnal Kimia Riset Vol. 3 No. 1 (2018): Juni
Publisher : Universitas Airlangga

Dengue virus distributed in tropical and subtropical regions in the world. DENV viruses are transmitted between humans primarily by Aedes aegypti and Aedes albopictus mosquitoes and are endemic in most areas in which the vectors occur. Four serotypes of dengue virus are DENV-1, DENV-2, DENV-3 and DENV-4. DENV-2 is comprised of six genotypes. Ethanol precipitation is a commonly used technique for concentrating and de-salting nucleic acids (DNA or RNA) preparations in aqueous solution. RNA isolation by combining Guanidinium thiocyanate and phenol reported has been reported. In this report, we investigated RNA isolation from DENV-2 using QIAamp Mini Kit with 2-Isopropanol, Methanol, Chloroform precipitation solvent. Electrophoregram showed DNA band as the result of RNA isolation with methanol and 2-isopropanol are produced quite well. Dna band of the of RNA isolation with chloroform solvent has the lowest intensity than methanol and 2-isopropanol. This study showed that methanol and 2-isopropanol can used as precipitation solvent for isolating RNA.

Precipitation Solvents for RNA Extraction of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, and Ultrapure H2O Rizqidhana Juliana Putri; Teguh Hari Sucipto; Harsasi Setyawati; Siti Churrotin; Ilham Harlan Amarullah; Puspa Wardhani; Aryati Aryati; Soegeng Soegijanto
Jurnal Kimia Riset Vol. 3 No. 2 (2018): Desember
Publisher : Universitas Airlangga

Dengue is a disease caused by a virus from the family Flaviviradae, carried by a female mosquito of Aedes aegypti species. Dengue fever is widespread in the tropic areas. It caused by rainfall, temperature and unplanned urbanization. According to the ministry of health , almost all provinces in Indonesia are endemic areas of dengue fever. In 2014, up to mid-December Dengue Hemorrhagic Fever (DHF) patients in 34 provinces in Indonesia are 71,668 people and 641. This figure is lower than the previous year, 2013 with 112,511 people and 871 deaths . This disease consists of four types of serotypes, namely DENV-1, DENV-2, DENV-3, and DENV-4. This disease can be identified using a variety of methods, one of the method is Reverse Transcription - Polymerase Chain Reaction (RT-PCR) method. This study aims to determine the ability of Dimethylformamide (DMF), Ethylenediamintetraacetic Acid (EDTA), and Ultrapure H2O as the substitute of Ethanol for precipitation in RNA extraction process. The sample used in this research obtained from Surabaya. RNA extraction itself can be done by using a special kit for RNA extraction. In Reverse Transcription - Polymerase Chain Reaction method, first RNA is extracted and then transcribed back (Reverse Transcription) which then form cDNA that later will be amplified by using PCR method. In this study used specific primers for dengue virus type 3 (DENV-3). The results of this study show that DMF, EDTA, and Ultrapure H2O can be used as the substitute of Ethanol for precipitation on RNA extraction. The result is evidenced by the formation of viral DNA bands on gel electrophoresis results.

Antibodies in Sera of Dengue Patients with Plasma Leakage Cross-Reacting with DENV Protein and Endothelial Protein Dewi Wulandari; Alida Roswita Harahap; Suhendro Suhendro; R. Tedjo Sasmono; Aryati Aryati; Herdiman Theodorus Pohan; Iris Rengganis; Saptawati Bardosono
The Indonesian Biomedical Journal Vol 14, No 1 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i1.1771

BACKGROUND: Dengue infection remains a major public health problem in Indonesia. Severe dengue associated with plasma leakage require hospitalization and potentially life threatening. However, the mechanism remains unclear, and the occurrence is unpredictable. The role of anti-endothelial antibody is predicted play an important role in the pathogenesis of plasma leakage, as severe dengue is more prevalent in secondary infection or post vaccinated individuals.METHODS: Serum samples from 127 single Dengue Virus (DENV) serotype infected subjects were obtained in day 2 of fever onset. Subjects were divided into plasma leakage and non-plasma leakage based on World Health Organization (WHO) criteria. Anti-endothelial antibody in patient sera were detected using western blot of Human Umbilical Vein Endothelial Cells (HUVEC). To confirm cross-reactivity, the sera was preabsorb with mix-DENV lysate.RESULTS: Three prominent bands were identified on western blot strips that inhibited by pre-absorption with DENV lysate. Plasma leakage patient expressed significantly more antibodies, with 51.7% of plasma leakage patients expressed at least two bands out of those three, compared to 18.5% of non-plasma leakage.CONCLUSION: Antibodies found in sera of dengue patients with plasma leakage cross-reacted with DENV proteins and endothelial proteins 37 kDa, 75 kDa, 120 kDa, and therefore may be involved in the pathogenesis of plasma leakage. Proteomic identification of those protein targets is needed and may be useful for vaccine studies and further development of predictor marker for plasma leakage in dengue.KEYWORDS: severe dengue, plasma leakage, cross-reactive, anti-endothelial antibody.

PROFILE OF DIABETIC KETOACIDOSIS PATIENTS AT REGIONAL PUBLIC HOSPITAL DR. SOETOMO IN 2017 Desak Gde Ushadi Bulan Dewata; Hermina Novida; Aryati Aryati
Jurnal Berkala Epidemiologi Vol. 8 No. 3 (2020): Jurnal Berkala Epidemiologi (Periodic Epidemiology Journal)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbe.V8I32020.301-309

Background: Diabetic ketoacidosis (DKA) is one of many life-threatening complications of diabetes mellitus (DM), which is characterized by severe hyperglycemia, metabolic acidosis and ketonemia. This complication can eventually lead to coma and death if not treated properly. Purpose: The aim of this study is to describe the profile of the DKA patients who were at Regional Public Hospital (RSUD) Dr. Soetomo in 2017. Methods: This study was a descriptive study that used the medical record data of DKA patients who were at RSUD Dr. Soetomo in 2017. The study’s total sample size was 63 patients. The study’s variables were gender, age, type of DM, severity of DKA, precipitating factors, main complaints, vital signs, random plasma glucose level, electrolyte level, and blood gas analysis. The frequency, mean, and standard deviation were analyzed. Results: Most of the 63 DKA patients were female (66.67%), aged from 50–59 years (38.10%). The DKA cases being handled by the Dr. Soetomo Hospital were overwhelmingly of type 2 DM patients (88.89%). More than half the patients (58.73%) experienced severe DKA. Altered states of consciousness (46.03%) and shortness of breath (26.98%) were the most common main complains made by the DKA patients. Almost all patients experienced infection (88.89%) as the precipitating factor; the most common infections were sepsis (92.86%), pneumonia (30.36%), and urinary tract infections (23.21%). Two thirds of the patients (66.67%) had a length of stay of 0–7 days. About 57.14% of the DKA patients died while undergoing treatment. Conclusion: The number of severe DKA patients and the mortality rate in our study were higher than in other studies. Females and the elderly were more prone to DKA, and the most common precipitating factor was infection.

Nilai Diagnostik Rapid Test TbAg dan MPT64 dengan Kultur Sebagai Gold Standard Muhammad Nazarudin; Jusak Nugraha; Aryati Aryati
Jurnal Biosains Pascasarjana Vol. 17 No. 3 (2015): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

AbstrakTuberkulosis (Tb) sampai kini masih dianggap sebagai salah satu penyakit berbahaya yang ada di muka bumi. Pada tahun 2012, diperkirakan 8,6 juta orang mengidap Tb dan 1,3 juta di antaranya meninggal dunia. Mycobacterium tuberculosis sebagai penyebab penyakit Tb, dapat dideteksi dengan pengecatan BTA untuk mewarnai bagian sitoplasma dari bakteri ini. Standar terbaik dalam pemeriksaan Tb adalah kultur bakteri, namun dinilai terlalu lama dalam memberikan hasil. Beberapa produsen mengembangkan alat yang mempermudah prosedur pemeriksaan, memberikan hasil yang cepat namun tetap berkualitas, diantaranya yang beredar di Indonesia adalah TbAg Rapid Test dan MPT64 Rapid Test. Metode penelitian ini adalah analitik observasional dengan rancangan cross sectional. Dilakukan terhadap 50 responden dengan 34 responden mengidap Tb paru. Data yang diuji nilai diagnostik, uji McNemar dan uji Kappa. Nilai sensitivitas, TbAg Rapid Test dengan sampel sputum 97.5% dan isolat 50%. Nilai sensitivitas MPT64 Rapid Test dengan sampel sputum 11.76% dan isolat 95%. Hasil BTA dan TbAg Rapid Test juga tidak menunjukkan perbedaan berarti sehingga dapat digunakan untuk pemeriksaan skrining dan MPT65 Rapid Test dapat gunakan untuk tes biokimia setelah kultur. Kata Kunci : Tuberkulosis, TbAg Rapid Test, MPT64 Rapid Test

The Relationship of Ns1 Antigen Profi Le and Days of Illness in Children with Dengue Virus Infection Dwiyanti Puspitasari; Saraswati Dewi; Aryati Aryati
Jurnal Ners Vol. 8 No. 1 (2013): April 2013
Publisher : Universitas Airlangga

Introduction: Dengue NS1 Antigen (NS1 Ag) detection or quantification has become a specific diagnostic tool for dengue virus infection, but has variable sensitivity. Previous research reported NS1 Ag level can be detected up to the 7th–10th day of fever, others stated the sensitivity was decreased after four days of fever. We performed this research to analyze the NS1 Ag profile and its relationship with the day of illness.Methods: This study was a prospective cohort study on 39 children hospitalized at Dr. Soetomo Hospital Surabaya since November 2009 to May 2010. Inclusion criteria were acute fever less than 72 hours, bleeding tendency denoted at least by positive tourniquet test, age between 1–14 years, and confirmed by positive dengue IgM/IgG on the 5th day of fever. We performed daily quantitative dengue NS1 Ag tests until defervescence day, and its relationship with the day of illness were analyzed.Results: NS1 Ag was positive in 19/39 samples. Secondary infection occurred in 14/19 of Dengue Fever and 17/20 of Dengue Hemorrhagic Fever cases. Positivity of NS1Ag was higher in primary (6/8 cases) than secondary infection (12/31 cases). Mean level of NS1 Ag was highest on the 2nd day, decreased afterwards and undetected on the 5th day of fever. There was significant relationship between NS1 Ag positivity (P=0.037, rs=-0.9) and level (P<0.001, rs=-1) with the day of illness.Conclusion: Dengue NS1 Ag positivity and level were highest during the acute phase of fever and decreased afterwards.The Relationship of Ns1 Antigen Profi Le and Days of Illness in Children with Dengue Virus Infection

Genetic Diversity of Plasmodium falciparum Glutamate Rich Protein in Patients Attending the Merauke Hospital in Papua Province, Indonesia Thomas Tandi Manu; Puspa Wardhani; Heny Arwati; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 27, No 2 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i2.1662

Malaria remains an important health problem in Indonesia with the highest transmission in Papua Province, an easternpart of this country. The genetic diversity of malaria parasites is the main problem in understanding several aspects ofmalaria infections and the dynamics of their transmission, which also play a role in the development of a vaccine.Plasmodium falciparum is the deadliest of the human malaria parasites. Plasmodium falciparum glutamate-rich protein(Pfglurp) is one of the many erythrocytic stages antigens currently under development for a vaccine. The Pfglurp gene hasbeen extensively used as a marker to investigate the genetic diversity, Multiplicity of Infection (MOI), the level of malariatransmission, immunity against malaria, as well as a discriminatory instrument to distinguish new from recrudescentinfections of the field parasite population. Thus, this genotyping study aimed to find out the genetic population ofP.falciparum at the Merauke District, Province of Papua, Indonesia. DNA samples were isolated from Dried Blood Spots(DBS) obtained from P.falciparum infected patients in the Regional Public Hospital of Merauke, Province of Papua, Indonesiaduring May 2019-July 2019. The isolated DNAs were then amplified for nested Polymerase Chain Reaction (PCR) prior toPfglurp genotyping. The glurp gene was identified in all 51 DBS samples of P.falciparum-infected patients, and 18 variants ofallele were found. Among them, 45.10% were found to bear multigenotype infections. The size of the dominant allele(12.5%) was 701-750 bp. The MOI was 1.58. The genetic population of P.falciparum in Merauke Hospital has contained ahigher percentage of multigenotypes compared with monogenotypes indicating the high transmission of malaria in thestudied area.

Correlation of Vitamin D and Calcium Levels in Children in New Diagnosed Epilepsy and Minimal 6 Months After Therapy Niluh Suwasanti; Aryati Aryati; Darto Saharso; Ferdy Royland Marpaung
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 1 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i1.1348

INTRODUCTION. Children with epilepsy should take long-term anti-epileptic drugs. Long-term use of anti-epileptic drugs can reduce vitamin D levels. Low vitamin D will lead to low blood calcium levels. This study aims to analyze the relationship between vitamin D and calcium levels in newly diagnosed epileptic children and ≥6 months after therapy. METHOD. These was an analytical observational study with cross sectional research design. The vitamin D examination instrument uses the ELFA method (enzyme linked fluorescent assay) with the Vidas instrument from bioMerieux. Samples were collected during June - August 2018 from Inpatient and Outpatient Clinics. The samples were divided into new diagnosis of epilepsy group and 6 months after therapy group. Each group was measured for vitamin D and serum calcium levels. The relationship between the two parameters were analyzed using T-Test independent. RESULTS. From the 19 new diagnosis of epilepsy, there were 57.9% low vitamin D and 10.5% low calcium levels. From the 20 subjects 6 months after therapy, 70% low vitamin D and 25% low calcium levels. There were a relationship between vitamin D and calcium levels in patients with newly diagnosed and ≥6 months after therapy. DISCUSSION. Low vitamin D and low calcium levels were found more in the anti-epileptic therapy group than the new diagnosis group of epilepsy. Low vitamin D levels can be caused by the use of long-term antiepileptic drugs that will affect serum calcium levels. CONCLUSION. This study showed a significant relationship between vitamin D and serum calcium levels in patients with newly diagnosed epilepsy and 6 months after therapy. Vitamin D and calsium serum examination should be done in every patient who gets long term therapy of antiepileptic drugs. KEY WORDS. Vitamin D, calcium, epilepsy, ELFA.

Co-Authors Agus Santosa Agus Sulistyono Agustin Iskandar Aksono HP., Eduardus Bimo Alida Roswita Harahap Anak Agung Wiradewi Lestari Andina Putri Aulia Andyanita Hanif Hermawati Anniwati, Leonita Aprilia, Andrea AR, M. Yazid Ariani, Grace Arifoel Hajat Bastiana Bastiana Budi Utomo Cavalier, Etienne Darto Saharso Desak Gde Ushadi Bulan Dewata Dewi Wulandari Djoko Santoso Doddy M. Soebadi Dominicus Husada Dwiyanti Puspitasari, Dwiyanti Eko Sulistijono Erawati Erawati Erni Juwita Nelwan, Erni Juwita Erwin Astha Triyono Fahimah Martak Ferdy Royland Marpaung Gondo Mastutik Handayani, Cut Fitri Harianto Notopuro Harsasi Setyawati Haryanto, Isnaeni Yudi Hebert Adrianto Heny Arwati Herdiman Theodorus Pohan Hermawati, Andayanita Hanif Hermina Novida, Hermina I A Putri Wirawati I Dewa Gede Ugrasena Ilham Harlan Amarullah Indah Susanti Iris Rengganis Isnaeni Isnaeni Yudi Haryanto Isnin Anang Marhana Jusak Nugraha Kris Cahyo Mulyatno, Kris Cahyo Kuntaman Kuntaman Kusmiati, Tutik Kusumastuti, Etty H. Laksita, Tetuka B. Lulut Kusumawati Lumban Toruan, Anggia Augustasia M. Andriady S. Nasution Maharani, Anisa Mardiyah, Nikmatul Margalin, Brilliant Marpaung, Ferdy Royland Masanori Kameoka, Masanori Masyeni, Sri Ma`ruf, Anwar Merylin Ranoko Mohammad Guritno Suryokusumo Mufasirin Muhammad Nazarudin Muhammad Rivai Mustika Amri Nabil Salim Ambar Nia Saurina Niluh Suwasanti Norwahyuni, Yuyun Nunki, Nastasya Nur Chamidah Nurdianto, Arif Rahman Patria Dewi, Pande Putu Ayu Perbowo, Primandono Pranidya, Nada Putri Purnomo, Windu Puspa Wardhani R Raharjo, Paulus R. Tedjo Sasmono Rahmawati, Lita Diah Retno Palupi Riries Rulaningtyas Rizaliansyah, Ferdian Rizqidhana Juliana Putri Rony, Zahara Tussoleha Royland Marpaung, Ferdy Rusli, Musofa Saptawati Bardosono Saraswati Dewi Sari, Arabella Vonia Sasmono, R. Tedjo Setianingsih, Yennie A. Shifa Fauziyah Shuhai Ueda Siti Churrotin, Siti Soegeng Soegijanto Sofro, Muchlis AU Sri Subekti Sri Sumarsih Suci Andriani Suhendro Suhendro SUKACITA TEHUPURING Sunari, I Gusti Agung Ayu Eka Putri Sunaryo Hardjowijoto Suprapto Maat Teguh Hari Sucipto, Teguh Hari Theresia Indah Budhy Sulisetyawati Thomas Tandi Manu Tjokroprawiro, Brahmana A. Tomohiro Kotaki, Tomohiro Trieva Verawaty Butarbutar Ueda, Shuhai Usman Hadi wahjoe djatisoesanto Wardhani, Puspa Wibrianto, Aswandi Widajati, Rahma Widodo J Pujiraharjo Yetti Hernaningsih Yohan, Benediktus Yovilianda Maulitiva Untoro Yulia Iriani, Yulia Yulia Nadar Indrasari Yuliasih

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